2,869 research outputs found
Stem cells and fluid flow drive cyst formation in an invertebrate excretory organ.
Cystic kidney diseases (CKDs) affect millions of people worldwide. The defining pathological features are fluid-filled cysts developing from nephric tubules due to defective flow sensing, cell proliferation and differentiation. The underlying molecular mechanisms, however, remain poorly understood, and the derived excretory systems of established invertebrate models (Caenorhabditis elegans and Drosophila melanogaster) are unsuitable to model CKDs. Systematic structure/function comparisons revealed that the combination of ultrafiltration and flow-associated filtrate modification that is central to CKD etiology is remarkably conserved between the planarian excretory system and the vertebrate nephron. Consistently, both RNA-mediated genetic interference (RNAi) of planarian orthologues of human CKD genes and inhibition of tubule flow led to tubular cystogenesis that share many features with vertebrate CKDs, suggesting deep mechanistic conservation. Our results demonstrate a common evolutionary origin of animal excretory systems and establish planarians as a novel and experimentally accessible invertebrate model for the study of human kidney pathologies
A feasibility study of signed consent for the collection of patient identifiable information for a national paediatric clinical audit database
Objectives: To investigate the feasibility of obtaining signed consent
for submission of patient identifiable data to a national clinical
audit database and to identify factors influencing the consent process
and its success.
Design: Feasibility study.
Setting: Seven paediatric intensive care units in England.
Participants: Parents/guardians of patients, or patients aged 12-16
years old, approached consecutively over three months for signed
consent for submission of patient identifiable data to the national
clinical audit database the Paediatric Intensive Care Audit Network
(PICANet).
Main outcome measures: The numbers and proportions of admissions for
which signed consent was given, refused, or not obtained (form not
returned or form partially completed but not signed), by age, sex,
level of deprivation, ethnicity (South Asian or not), paediatric index
of mortality score, length of hospital stay (days in paediatric
intensive care).
Results: One unit did not start and one did not fully implement the
protocol, so analysis excluded these two units. Consent was obtained
for 182 of 422 admissions (43%) (range by unit 9% to 84%). Most
(101/182; 55%) consents were taken by staff nurses. One refusal (0.2%)
was received. Consent rates were significantly better for children who
were more severely ill on admission and for hospital stays of six days
or more, and significantly poorer for children aged 10-14 years. Long
hospital stays and children aged 10-14 years remained significant in a
stepwise regression model of the factors that were significant in the
univariate model.
Conclusion: Systematically obtaining individual signed consent for
sharing patient identifiable information with an externally located
clinical audit database is difficult. Obtaining such consent is
unlikely to be successful unless additional resources are specifically
allocated to training, staff time, and administrative support
A Note on the Slim Accretion Disk Model
We show that when the gravitational force is correctly calculated in dealing
with the vertical hydrostatic equilibrium of black hole accretion disks, the
relationship that is valid for geometrically thin disks, i.e., constant, where is the sound speed, is the Keplerian
angular velocity, and is the half-thickness of the disk, does not hold for
slim disks. More importantly, by adopting the correct vertical gravitational
force in studies of thermal equilibrium solutions, we find that there exists a
maximally possible accretion rate for each radius in the outer region of
optically thick accretion flows, so that only the inner region of these flows
can possibly take the form of slim disks, and strong outflows from the outer
region are required to reduce the accretion rate in order for slim disks to be
realized.Comment: 14 pages, 5 figures, accepted by Ap
Numerical Models of Viscous Accretion Flows Near Black Holes
We report on a numerical study of viscous fluid accretion onto a black hole.
The flow is axisymmetric and uses a pseudo-Newtonian potential to model
relativistic effects near the event horizon. The numerical method is a variant
of the ZEUS code. As a test of our numerical scheme, we are able to reproduce
results from earlier, similar work by Igumenshchev and Abramowicz and Stone et
al. We consider models in which mass is injected onto the grid as well as
models in which an initial equilibrium torus is accreted. In each model we
measure three ``eigenvalues'' of the flow: the accretion rate of mass, angular
momentum, and energy. We find that the eigenvalues are sensitive to r_{in}, the
location of the inner radial boundary. Only when the flow is always supersonic
on the inner boundary are the eigenvalues insensitive to small changes in
r_{in}. We also report on the sensitivity of the results to other numerical
parameters.Comment: 14 pages, 4 figures, 2 tables, to appear in v573 n2 pt1 ApJ July 10,
200
A PC parallel port button box provides millisecond response time accuracy under Linux
For psychologists, it is sometimes necessary to measure people's reaction times to the nearest millisecond. This article describes how to use the PC parallel port to receive signals from a button box to achieve millisecond response time accuracy. The workings of the parallel port, the corresponding port addresses, and a simple Linux program for controlling the port are described. A test of the speed and reliability of button box signal detection is reported. If the reader is moderately familiar with Linux, this article should provide sufficient instruction for him or her to build and test his or her own parallel port button box. This article also describes how the parallel port could be used to control an external apparatus
Comparison of 35 and 50 {\mu}m thin HPK UFSD after neutron irradiation up to 6*10^15 neq/cm^2
We report results from the testing of 35 {\mu}m thick Ultra-Fast Silicon
Detectors (UFSD produced by Hamamatsu Photonics (HPK), Japan and the comparison
of these new results to data reported before on 50 {\mu}m thick UFSD produced
by HPK. The 35 {\mu}m thick sensors were irradiated with neutrons to fluences
of 0, 1*10^14, 1*10^15, 3*10^15, 6*10^15 neq/cm^2. The sensors were tested
pre-irradiation and post-irradiation with minimum ionizing particles (MIPs)
from a 90Sr \b{eta}-source. The leakage current, capacitance, internal gain and
the timing resolution were measured as a function of bias voltage at -20C and
-27C. The timing resolution was extracted from the time difference with a
second calibrated UFSD in coincidence, using the constant fraction method for
both. Within the fluence range measured, the advantage of the 35 {\mu}m thick
UFSD in timing accuracy, bias voltage and power can be established.Comment: 9 pages, 9 figures, HSTD11 Okinawa. arXiv admin note: text overlap
with arXiv:1707.0496
The rarity of terrestrial gamma-ray flashes
We report on the first search for Terrestrial Gamma-ray Flashes (TGFs) from altitudes where they are thought to be produced. The Airborne Detector for Energetic Lightning Emissions (ADELE), an array of gamma-ray detectors, was flown near the tops of Florida thunderstorms in August/September 2009. The plane passed within 10 km horizontal distance of 1213 lightning discharges and only once detected a TGF. If these discharges had produced TGFs of the same intensity as those seen from space, every one should have been seen by ADELE. Separate and significant nondetections are established for intracloud lightning, negative cloud-to-ground lightning, and narrow bipolar events. We conclude that TGFs are not a primary triggering mechanism for lightning. We estimate the TGF-to-flash ratio to be on the order of 10^(â2) to 10^(â3) and show that TGF intensities cannot follow the well-known power-law distribution seen in earthquakes and solar flares, due to our limits on the presence of faint events
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Epigenetic memory in induced pluripotent stem cells.
Somatic cell nuclear transfer and transcription-factor-based reprogramming revert adult cells to an embryonic state, and yield pluripotent stem cells that can generate all tissues. Through different mechanisms and kinetics, these two reprogramming methods reset genomic methylation, an epigenetic modification of DNA that influences gene expression, leading us to hypothesize that the resulting pluripotent stem cells might have different properties. Here we observe that low-passage induced pluripotent stem cells (iPSCs) derived by factor-based reprogramming of adult murine tissues harbour residual DNA methylation signatures characteristic of their somatic tissue of origin, which favours their differentiation along lineages related to the donor cell, while restricting alternative cell fates. Such an 'epigenetic memory' of the donor tissue could be reset by differentiation and serial reprogramming, or by treatment of iPSCs with chromatin-modifying drugs. In contrast, the differentiation and methylation of nuclear-transfer-derived pluripotent stem cells were more similar to classical embryonic stem cells than were iPSCs. Our data indicate that nuclear transfer is more effective at establishing the ground state of pluripotency than factor-based reprogramming, which can leave an epigenetic memory of the tissue of origin that may influence efforts at directed differentiation for applications in disease modelling or treatment
Legacy and Novel Per- and Polyfluoroalkyl Substances in Juvenile Seabirds from the U.S. Atlantic Coast
Per- and polyfluoroalkyl substances (PFAS) are anthropogenic, globally distributed chemicals. Legacy PFAS, including perfluorooctane sulfonate (PFOS), have been regularly detected in marine fauna but little is known about their current levels or the presence of novel PFAS in seabirds. We measured 36 emerging and legacy PFAS in livers from 31 juvenile seabirds from Massachusetts Bay, Narragansett Bay, and the Cape Fear River Estuary (CFRE), United States. PFOS was the major legacy perfluoroalkyl acid present, making up 58% of concentrations observed across all habitats (range: 11â280 ng/g). Novel PFAS were confirmed in chicks hatched downstream of a fluoropolymer production site in the CFRE: a perfluorinated ether sulfonic acid (Nafion byproduct 2; range: 1â110 ng/g) and two perfluorinated ether carboxylic acids (PFO4DA and PFO5DoDA; PFO5DoDA range: 5â30 ng/g). PFOS was inversely associated with phospholipid content in livers from CFRE and Massachusetts Bay individuals, while ÎŽ 13C, an indicator of marine versus terrestrial foraging, was positively correlated with some long-chain PFAS in CFRE chick livers. There is also an indication that seabird phospholipid dynamics are negatively impacted by PFAS, which should be further explored given the importance of lipids for seabirds
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