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Suspect screening of maternal serum to identify new environmental chemical biomonitoring targets using liquid chromatography-quadrupole time-of-flight mass spectrometry.
The use and advantages of high-resolution mass spectrometry (MS) as a discovery tool for environmental chemical monitoring has been demonstrated for environmental samples but not for biological samples. We developed a method using liquid chromatography-quadrupole time-of-flight MS (LC-QTOF/MS) for discovery of previously unmeasured environmental chemicals in human serum. Using non-targeted data acquisition (full scan MS analysis) we were able to screen for environmental organic acids (EOAs) in 20 serum samples from second trimester pregnant women. We define EOAs as environmental organic compounds with at least one dissociable proton which are utilized in commerce. EOAs include environmental phenols, phthalate metabolites, perfluorinated compounds, phenolic metabolites of polybrominated diphenyl ethers and polychlorinated biphenyls, and acidic pesticides and/or predicted acidic pesticide metabolites. Our validated method used solid phase extraction, reversed-phase chromatography in a C18 column with gradient elution, electrospray ionization in negative polarity and automated tandem MS (MS/MS) data acquisition to maximize true positive rates. We identified "suspect EOAs" using Agilent MassHunter Qualitative Analysis software, to match chemical formulas generated from each sample run with molecular formulas in our unique database of 693 EOAs assembled from multiple environmental literature sources. We found potential matches for 282 (41%) of the EOAs in our database. Sixty-five of these suspect EOAs were detected in at least 75% of the samples; only 19 of these compounds are currently biomonitored in National Health and Nutrition Examination Survey. We confirmed two of three suspect EOAs by LC-QTOF/MS using a targeted method developed through LC-MS/MS, reporting the first confirmation of benzophenone-1 and bisphenol S in pregnant women's sera. Our suspect screening workflow provides an approach to comprehensively scan environmental chemical exposures in humans. This can provide a better source of exposure information to help improve exposure and risk evaluation of industrial chemicals
Spin exchange in quantum rings and wires in the Wigner-crystal limit
We present a controlled method for computing the exchange coupling in
strongly correlated one-dimensional electron systems. It is based on the
asymptotically exact relation between the exchange constant and the
pair-correlation function of spinless electrons. Explicit results are obtained
for thin quantum rings with realistic Coulomb interactions, by calculating this
function via a many-body instanton approach.Comment: 7 pages, 2 figures. Changes in the text and figures to improve
readability; added reference
Phylogeny and New Intrageneric Classification of Allium (Alliaceae) Based on Nuclear Ribosomal DNA ITS Sequences
The internal transcribed spacer region (ITS) of nuclear ribosomal DNA was sequenced from 195 representative species of Allium, two species of Nothoscordum, and one species each of lpheion, Dichelostemma, and Tulbaghia. Within the Allium species the lengths of the ITS regions were in a range from 612 to 661 base pairs and pairwise genetic distances reached up to 46%. The ITS data supported the inclusion of Nectaroscordum, Caloscordum, and Milula into Allium. Subgenera Rhizirideum and Allium, as well as sects. Reticulatobulbosa and Oreiprason were non-monophyletic taxa. Based on the phylogenetic relations, a new classification of genus Allium consisting of 15 monophyletic subgenera is presented. Sections Microscordum, Anguinum, Porphyroprason, Vvedenskya, Butomissa, Cyathophora, and Reticulatobulbosa are raised to subgeneric rank. Sections Austromontana N. Friesen, Eduardia N. Friesen, Mediasia F. O. Khassanov, S. C. Yengalycheva et N. Friesen, Nigrimontana N. Friesen, Falcatifolia N. Friesen, and Condensatum N. Friesen are newly described. Series Daghestanica, Pallasia, and Scabriscapa, as well as subsects. Eremoprasum, Longivaginata, and Sikkimensia are raised to sectional rank. A taxonomic conspectus of Allium at sectional level is given
Learning from the early adopters: developing the digital practitioner
This paper explores how Sharpe and Beetham’s Digital Literacies Framework which was derived to model students’ digital literacies, can be applied to lecturers’ digital literacy practices. Data from a small-scale phenomenological study of higher education lecturers who used Web 2.0 in their teaching and learning practices are used to examine if this pyramid model represents their motivations for adopting technology-enhanced learning in their pedagogic practices. The paper argues that whilst Sharpe and Beetham’s model has utility in many regards, these lecturers were mainly motivated by the desire to achieve their pedagogic goals rather than by a desire to become a digital practitioner
VLA Survey of Dense Gas in Extended Green Objects: Prevalence of 25 GHz Methanol Masers
We present resolution Very Large Array (VLA) observations of four
CHOH - 25~GHz transitions (=3, 5, 8, 10) along with 1.3~cm
continuum toward 20 regions of active massive star formation containing
Extended Green Objects (EGOs), 14 of which we have previously studied with the
VLA in the Class~I 44~GHz and Class~II 6.7~GHz maser lines (Cyganowski et al.
2009). Sixteen regions are detected in at least one 25~GHz line (=5), with
13 of 16 exhibiting maser emission. In total, we report 34 new sites of
CHOH maser emission and ten new sites of thermal CHOH emission,
significantly increasing the number of 25~GHz Class I CHOH masers observed
at high angular resolution. We identify probable or likely maser counterparts
at 44~GHz for all 15 of the 25~GHz masers for which we have complementary data,
providing further evidence that these masers trace similar physical conditions
despite uncorrelated flux densities. The sites of thermal and maser emission of
CHOH are both predominantly associated with the 4.5 m emission from
the EGO, and the presence of thermal CHOH emission is accompanied by 1.3~cm
continuum emission in 9 out of 10 cases. Of the 19 regions that exhibit 1.3~cm
continuum emission, it is associated with the EGO in 16 cases (out of a total
of 20 sites), 13 of which are new detections at 1.3~cm. Twelve of the 1.3~cm
continuum sources are associated with 6.7~GHz maser emission and likely trace
deeply-embedded massive protostars
Second Harmonic Coherent Driving of a Spin Qubit in a Si/SiGe Quantum Dot
We demonstrate coherent driving of a single electron spin using second
harmonic excitation in a Si/SiGe quantum dot. Our estimates suggest that the
anharmonic dot confining potential combined with a gradient in the transverse
magnetic field dominates the second harmonic response. As expected, the Rabi
frequency depends quadratically on the driving amplitude and the periodicity
with respect to the phase of the drive is twice that of the fundamental
harmonic. The maximum Rabi frequency observed for the second harmonic is just a
factor of two lower than that achieved for the first harmonic when driving at
the same power. Combined with the lower demands on microwave circuitry when
operating at half the qubit frequency, these observations indicate that second
harmonic driving can be a useful technique for future quantum computation
architectures.Comment: 9 pages, 9 figure
Identifying single electron charge sensor events using wavelet edge detection
The operation of solid-state qubits often relies on single-shot readout using
a nanoelectronic charge sensor, and the detection of events in a noisy sensor
signal is crucial for high fidelity readout of such qubits. The most common
detection scheme, comparing the signal to a threshold value, is accurate at low
noise levels but is not robust to low-frequency noise and signal drift. We
describe an alternative method for identifying charge sensor events using
wavelet edge detection. The technique is convenient to use and we show that,
with realistic signals and a single tunable parameter, wavelet detection can
outperform thresholding and is significantly more tolerant to 1/f and
low-frequency noise.Comment: 11 pages, 4 figure
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