Phage therapy in veterinary medicine

To overcome the obstacle of antimicrobial resistance, researchers are investigating the use of phage therapy as an alternative and/or supplementation to antibiotics to treat and prevent infections both in humans and in animals. In the first part of this review, we describe the unique biological characteristics of bacteriophages and the crucial aspects influencing the success of phage therapy. However, despite their efficacy and safety, there is still no specific legislation that regulates their use. In the second part of this review, we describe the comprehensive research done in the past and recent years to address the use of phage therapy for the treatment and prevention of bacterial disease affecting domestic animals as an alternative to antibiotic treatments. While in farm animals, phage therapy efficacy perspectives have been widely studied in vitro and in vivo, especially for zoonoses and diseases linked to economic losses (such as mastitis), in pets, studies are still few and rather recent

The First Record of a Pre-Columbian Domestic Dog (Canis lupus familiaris) in Brazil

Archaeological excavations of the PSG-07 earthen mound at Pontal da Barra in Rio Grande do Sul, southern Brazil have revealed the earliest known evidence for the presence of domestic dog (Canis lupus familiaris) in Brazil. This is the first reported pre-Columbian example in the country. Analysis of morphology, morphometry, and dental enamel laminae identified a left maxillary molar 1, left maxillary molar 2 and attached fragments of the maxilla of C.l.familiaris. A direct radiocarbon date on a fragment of the maxilla provided an age range between 1701 and 1526cal BP (2σ). This is within the range of other dates for the site, which indicate intermittent occupation between 2024 and 1027cal BP (2σ). Data from carbon isotope analysis indicates a potential marine diet. However, nitrogen isotope analysis values are lower than expected for a marine diet. The sparse records of pre-Columbian C. l.familiaris in the region emphasise the importance of the present work.Fil: Guedes Milheira, R.. Universidade Federal de Pelotas; BrasilFil: Loponte, Daniel Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; ArgentinaFil: García Esponda, C.. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Acosta, Alejandro Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; ArgentinaFil: Ulguim, P.. Teesside Univeristy; Reino Unid

The First Record of a Pre-Columbian Domestic Dog (<i>Canis lupus familiaris</i>) in Brazil

Archaeological excavations of the PSG-07 earthen mound at Pontal da Barra in Rio Grande do Sul, southern Brazil have revealed the earliest known evidence for the presence of domestic dog (Canis lupus familiaris) in Brazil. This is the first reported pre-Columbian example in the country. Analysis of morphology, morphometry, and dental enamel laminae identified a left maxillary molar 1, left maxillary molar 2 and attached fragments of the maxilla of C.l.familiaris. A direct radiocarbon date on a fragment of the maxilla provided an age range between 1701 and 1526cal BP (2σ). This is within the range of other dates for the site, which indicate intermittent occupation between 2024 and 1027cal BP (2σ). Data from carbon isotope analysis indicates a potential marine diet. However, nitrogen isotope analysis values are lower than expected for a marine diet. The sparse records of pre-Columbian C. l.familiaris in the region emphasise the importance of the present work.Facultad de Ciencias Naturales y Muse

The First Record of a Pre-Columbian Domestic Dog (<i>Canis lupus familiaris</i>) in Brazil

Archaeological excavations of the PSG-07 earthen mound at Pontal da Barra in Rio Grande do Sul, southern Brazil have revealed the earliest known evidence for the presence of domestic dog (Canis lupus familiaris) in Brazil. This is the first reported pre-Columbian example in the country. Analysis of morphology, morphometry, and dental enamel laminae identified a left maxillary molar 1, left maxillary molar 2 and attached fragments of the maxilla of C.l.familiaris. A direct radiocarbon date on a fragment of the maxilla provided an age range between 1701 and 1526cal BP (2σ). This is within the range of other dates for the site, which indicate intermittent occupation between 2024 and 1027cal BP (2σ). Data from carbon isotope analysis indicates a potential marine diet. However, nitrogen isotope analysis values are lower than expected for a marine diet. The sparse records of pre-Columbian C. l.familiaris in the region emphasise the importance of the present work.Facultad de Ciencias Naturales y Muse

Synergistic roles of climate warming and human occupation in Patagonian megafaunal extinctions during the Last Deglaciation.

The causes of Late Pleistocene megafaunal extinctions (60,000 to 11,650 years ago, hereafter 60 to 11.65 ka) remain contentious, with major phases coinciding with both human arrival and climate change around the world. The Americas provide a unique opportunity to disentangle these factors as human colonization took place over a narrow time frame (~15 to 14.6 ka) but during contrasting temperature trends across each continent. Unfortunately, limited data sets in South America have so far precluded detailed comparison. We analyze genetic and radiocarbon data from 89 and 71 Patagonian megafaunal bones, respectively, more than doubling the high-quality Pleistocene megafaunal radiocarbon data sets from the region. We identify a narrow megafaunal extinction phase 12,280 ± 110 years ago, some 1 to 3 thousand years after initial human presence in the area. Although humans arrived immediately prior to a cold phase, the Antarctic Cold Reversal stadial, megafaunal extinctions did not occur until the stadial finished and the subsequent warming phase commenced some 1 to 3 thousand years later. The increased resolution provided by the Patagonian material reveals that the sequence of climate and extinction events in North and South America were temporally inverted, but in both cases, megafaunal extinctions did not occur until human presence and climate warming coincided. Overall, metapopulation processes involving subpopulation connectivity on a continental scale appear to have been critical for megafaunal species survival of both climate change and human impacts

Dynamic phosphorylation of Histone Deacetylase 1 by Aurora kinases during mitosis regulates zebrafish embryos development

Histone deacetylases (HDACs) catalyze the removal of acetyl molecules from histone and nonhistone substrates playing important roles in chromatin remodeling and control of gene expression. Class I HDAC1 is a critical regulator of cell cycle progression, cellular proliferation and differentiation during development; it is also regulated by many post-translational modifications (PTMs). Herein we characterize a new mitosis-specific phosphorylation of HDAC1 driven by Aurora kinases A and B. We show that this phosphorylation affects HDAC1 enzymatic activity and it is critical for the maintenance of a proper proliferative and developmental plan in a complex organism. Notably, we find that Aurora-dependent phosphorylation of HDAC1 regulates histone acetylation by modulating the expression of genes directly involved in the developing zebrafish central nervous system. Our data represent a step towards the comprehension of HDAC1 regulation by its PTM code, with important implications in unravelling its roles both in physiology and pathology

Synergistic roles of climate warming and human occupation in Patagonian megafaunal extinctions during the Last Deglaciation

The causes of Late Pleistocene megafaunal extinctions (60,000 to 11,650 years ago, hereafter 60 to 11.65 ka) remain contentious, with major phases coinciding with both human arrival and climate change around the world. The Americas provide a unique opportunity to disentangle these factors as human colonization took place over a narrow time frame (~15 to 14.6 ka) but during contrasting temperature trends across each continent. Unfortunately, limited data sets in South America have so far precluded detailed comparison. We analyze genetic and radiocarbon data from 89 and 71 Patagonian megafaunal bones, respectively, more than doubling the high-quality Pleistocene megafaunal radiocarbon data sets from the region. We identify a narrow megafaunal extinction phase 12,280 ± 110 years ago, some 1 to 3 thousand years after initial human presence in the area. Although humans arrived immediately prior to a cold phase, the Antarctic Cold Reversal stadial, megafaunal extinctions did not occur until the stadial finished and the subsequent warming phase commenced some 1 to 3 thousand years later. The increased resolution provided by the Patagonian material reveals that the sequence of climate and extinction events in North and South America were temporally inverted, but in both cases, megafaunal extinctions did not occur until human presence and climate warming coincided. Overall, metapopulation processes involving subpopulation connectivity on a continental scale appear to have been critical for megafaunal species survival of both climate change and human impacts.Jessica L. Metcalf, Chris Turney, Ross Barnett, Fabiana Martin, Sarah C. Bray, Julia T. Vilstrup, Ludovic Orlando, Rodolfo Salas-Gismondi, Daniel Loponte, Matías Medina, Mariana De Nigris, Teresa Civalero, Pablo Marcelo Fernández, Alejandra Gasco, Victor Duran, Kevin L. Seymour, Clara Otaola, Adolfo Gil, Rafael Paunero, Francisco J. Prevosti, Corey J. A. Bradshaw, Jane C. Wheeler, Luis Borrero, Jeremy J. Austin, Alan Coope