518 research outputs found
X-ray Spectroscopy of Candidate Ultracompact X-ray Binaries
We present high-resolution spectroscopy of the neutron star/low-mass X-ray
binaries (LMXBs) 4U 1850-087 and 4U 0513-40 as part of our continuing study of
known and candidate ultracompact binaries. The LMXB 4U 1850-087 is one of four
systems in which we had previously inferred an unusual Ne/O ratio in the
absorption along the line of sight, most likely from material local to the
binaries. However, our recent Chandra X-ray Observatory LETGS spectrum of 4U
1850-087 finds a Ne/O ratio by number of 0.22+/-0.05, smaller than previously
measured and consistent with the expected interstellar value. We propose that
variations in the Ne/O ratio due to source variability, as previously observed
in these sources, can explain the difference between the low- and
high-resolution spectral results for 4U 1850-087. Our XMM-Newton RGS
observation of 4U 0513-40 also shows no unusual abundance ratios in the
absorption along the line of sight. We also present spectral results from a
third candidate ultracompact binary, 4U 1822-000, whose spectrum is well fit by
an absorbed power-law + blackbody model with absorption consistent with the
expected interstellar value. Finally, we present the non-detection of a fourth
candidate ultracompact binary, 4U 1905+000, with an upper limit on the source
luminosity of < 1 x 10^{32} erg s^{-1}. Using archival data, we show that the
source has entered an extended quiescent state.Comment: 8 pages, 3 figures, accepted for publication to the Astrophysical
Journa
Intracellular sodium changes in cancer cells using a microcavity array-based bioreactor system and sodium triple-quantum mr signal
The sodium triple-quantum (TQ) magnetic resonance (MR) signal created by interactions of sodium ions with macromolecules has been demonstrated to be a valuable biomarker for cell viability. The aim of this study was to monitor a cellular response using the sodium TQ signal during inhibition of Na/K-ATPase in living cancer cells (HepG2). The cells were dynamically investigated after exposure to 1 mM ouabain or K-free medium for 60 min using an MR-compatible bioreactor system. An improved TQ time proportional phase incrementation (TQTPPI) pulse sequence with almost four times TQ signal-to-noise ratio (SNR) gain allowed for conducting experiments with 12–14 × 10 cells using a 9.4 T MR scanner. During cell intervention experiments, the sodium TQ signal increased to 138.9 ± 4.1% and 183.4 ± 8.9% for 1 mM ouabain (n = 3) and K-free medium (n = 3), respectively. During reperfusion with normal medium, the sodium TQ signal further increased to 169.2 ± 5.3% for the ouabain experiment, while it recovered to 128.5 ± 6.8% for the K-free experiment. These sodium TQ signal increases agree with an influx of sodium ions during Na/K-ATPase inhibition and hence a reduced cell viability. The improved TQ signal detection combined with this MR-compatible bioreactor system provides a capability to investigate the cellular response of a variety of cells using the sodium TQ MR signal
Mass Spectrometry Imaging Disclosed Spatial Distribution of Defense-Related Metabolites in Triticum spp
Fusarium Head Blight is the most common fungal disease that strongly affects Triticum spp., reducing crop yield and leading to the accumulation of toxic metabolites. Several studies have investigated the plant metabolic response to counteract mycotoxins accumulation. However, information on the precise location where the defense mechanism is taking place is scarce. Therefore, this study aimed to investigate the specific tissue distribution of defense metabolites in two Triticum species and use this information to postulate on the metabolites’ functional role, unlocking the “location-to-function” paradigm. To address this challenge, transversal cross-sections were obtained from the middle of the grains. They were analyzed using an atmospheric-pressure (AP) SMALDI MSI source (AP-SMALDI5 AF, TransMIT GmbH, Giessen, Germany) coupled to a Q Exactive HF (Thermo Fisher Scientific GmbH, Bremen, Germany) orbital trapping mass spectrometer. Our result revealed the capability of (AP)-SMALDI MSI instrumentation to finely investigate the spatial distribution of wheat defense metabolites, such as hydroxycinnamic acid amides, oxylipins, linoleic and α-linoleic acids, galactolipids, and glycerolipids
4U2206+54 - an Unusual High Mass X-ray Binary with a 9.6 Day Orbital Period but No Strong Pulsations
Rossi X-ray Timing Explorer All-Sky Monitor observations of the X-ray source
4U2206+54, previously proposed to be a Be star system, show the X-ray flux to
be modulated with a period of approximately 9.6 days. If the modulation is due
to orbital variability then this would be one of the shortest orbital periods
known for a Be star X-ray source. However, the X-ray luminosity is relatively
modest whereas a high luminosity would be predicted if the system contains a
neutron star accreting from the denser inner regions of a Be star envelope.
Although a 392s pulse period was previously reported from EXOSAT observations,
a reexamination of the EXOSAT light curves does not show this or any other
periodicity. An analysis of archival RXTE Proportional Counter Array
observations also fails to show any X-ray pulsations. We consider possible
models that may explain the properties of this source including a neutron star
with accretion halted at the magnetosphere and an accreting white dwarf.Comment: Accepted for publication in the Astrophysical Journa
A Comparison of Some Fuzzy Relation-based Linguistic Preference Models for Multiple-Factor Project Assessment
Some approaches to the use of linguistic-preference models based on fuzzy relations in the context of multiple factor project assessment are considered. Projects are characterized in terms of linguistic expressions of 'performance' with respect to factors or impacts and the 'importance' of those factors and impacts. Some variations of methods by Wilhelm and Parsaei (1991) and Eldukair and Ayyub (1992) are considered with some possible analogous methods. A simple illustrative, hypothetical example is developed to compare methods in the context of a proposed bridge river crossing in the city of Brisbane, Queensland, Australia, assessed against six factors: (1) cost, (2) lifespan, (3) usage, (4) aesthetics, (5) construction time, and (6) environmental impact
X-ray Variability in the Young Massive Triple theta2 Ori A
Massive stars rarely show intrinsic X-ray variability. The only O-stars
credited to be intrinsically variable are theta1 Ori C due to effects from
magnetic confinement of its wind, and theta2 Ori A suspected of similar
activity. Early Chandra observations have shown that the most massive star
system in the Orion Trapezium Cluster, theta2 Ori A, shows rapid variability on
time scales of hours. We determine X-ray fluxes and find that the star shows
very strong variability over the last 5 years. We observed a second large X-ray
outburst in November 2004 with the high resolution transmission grating
spectrometer on-board Chandra. In the low state X-ray emissivities indicate
temperatures well above 25 MK. In the high state we find an extended emissivity
distribution with high emissivities in the range from 3 MK to over 100 MK. The
outburst event in stellar terms is one of the most powerful ever observed and
the most energetic one in the ONC with a lower total energy limit of 1.5x10^37
ergs. The line diagnostics show that under the assumption that the line
emitting regions in the low states are as close as within 1 -- 2 stellar radii
from the O-star's photosphere, whereas the hard states suggest a distance of 3
-- 5 stellar radii. The two outbursts are very close to the periastron passage
of the stars. We argue that the high X-ray states are possibly the result of
reconnection events from magnetic interactions of the primary and secondary
stars of the spectroscopic binary. Effects from wind collisions seem unlikely
for this system. The low state emissivity and R-ratios strengthen the
predicament that the X-ray emission is enhanced by magnetic confinement of the
primary wind. We also detect Fe fluorescence indicative of the existence of
substantial amounts of neutral Fe in the vicinity of the X-ray emission.Comment: 11 pages, 8 figures, accepted for publication in The Astrophysical
Main Journa
Resonance ionization spectroscopy of thorium isotopes - towards a laser spectroscopic identification of the low-lying 7.6 eV isomer of Th-229
In-source resonance ionization spectroscopy was used to identify an efficient
and selective three step excitation/ionization scheme of thorium, suitable for
titanium:sapphire (Ti:sa) lasers. The measurements were carried out in
preparation of laser spectroscopic investigations for an identification of the
low-lying Th-229m isomer predicted at 7.6 +- 0.5 eV above the nuclear ground
state. Using a sample of Th-232, a multitude of optical transitions leading to
over 20 previously unknown intermediate states of even parity as well as
numerous high-lying odd parity auto-ionizing states were identified. Level
energies were determined with an accuracy of 0.06 cm-1 for intermediate and
0.15 cm-1 for auto-ionizing states. Using different excitation pathways an
assignment of total angular momenta for several energy levels was possible. One
particularly efficient ionization scheme of thorium, exhibiting saturation in
all three optical transitions, was studied in detail. For all three levels in
this scheme, the isotope shifts of the isotopes Th-228, Th-229, and Th-230
relative to Th-232 were measured. An overall efficiency including ionization,
transport and detection of 0.6 was determined, which was predominantly limited
by the transmission of the mass spectrometer ion optics
The cellular heat shock response monitored by chemical exchange saturation transfer MRI
CEST-MRI of the rNOE signal has been demonstrated in vitro to be closely linked to the protein conformational state. As the detectability of denaturation and aggregation processes on a physiologically relevant scale in living organisms has yet to be verified, the aim of this study was to perform heat-shock experiments with living cells to monitor the cellular heat-shock response of the rNOE CEST signal. Cancer cells (HepG2) were dynamically investigated after a mild, non-lethal heat-shock of 42 °C for 20 min using an MR-compatible bioreactor system at 9.4 T. Reliable and fast high-resolution CEST imaging was realized by a relaxation-compensated 2-point contrast metric. After the heat-shock, a substantial decrease of the rNOE CEST signal by 8.0 ± 0.4% followed by a steady signal recovery within a time of 99.1 ± 1.3 min was observed in two independent trials. This continuous signal recovery is in coherence with chaperone-induced refolding of heat-shock induced protein aggregates. We demonstrated that protein denaturation processes influence the CEST-MRI signal on a physiologically relevant scale. Thus, the protein folding state is, along with concentration changes, a relevant physiological parameter for the interpretation of CEST signal changes in diseases that are associated with pathological changes in protein expression, like cancer and neurodegenerative diseases
Regular and chaotic vibration in a piezoelectric energy harvester
We examine regular and chaotic responses of a vibrational energy harvester composed of a vertical beam and a tip mass. The beam is excited horizontally by a harmonic inertial force while mechanical vibrational energy is converted to electrical power through a piezoelectric patch. The mechanical resonator can be described by single or double well potentials depending on the gravity force from the tip mass. By changing the tip mass we examine bifurcations from single well oscillations, to regular and chaotic vibrations between the potential wells. The appearance of chaotic responses in the energy harvesting system is illustrated by the bifurcation diagram, the corresponding Fourier spectra, the phase portraits, and is confirmed by the 0–1 test. The appearance of chaotic vibrations reduces the level of harvested energy
A Cellular Automata Model for Citrus Variagated Chlorosis
A cellular automata model is proposed to analyze the progress of Citrus
Variegated Chlorosis epidemics in S\~ao Paulo oranges plantation. In this model
epidemiological and environmental features, such as motility of sharpshooter
vectors which perform L\'evy flights, hydric and nutritional level of plant
stress and seasonal climatic effects, are included. The observed epidemics data
were quantitatively reproduced by the proposed model varying the parameters
controlling vectors motility, plant stress and initial population of diseased
plants.Comment: 10 pages, 10 figures, Scheduled tentatively for the issue of: 01Nov0
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