49 research outputs found
Local Ferroelectricity in SrTiO_3 Thin Films
The temperature-dependent polarization of SrTiO_3 thin films is investigated
using confocal scanning optical microscopy. A homogeneous out-of-plane and
inhomogeneous in-plane ferroelectric phase are identified from images of the
linear electrooptic response. Both hysteretic and non-hysteretic behavior are
observed under a dc bias field. Unlike classical transitions in bulk
ferroelectrics, local ferroelectricity is observed at temperatures far above
the dielectric permittivity maximum. The results demonstrate the utility of
local probe experiments in understanding inhomogeneous ferroelectrics.Comment: 8 pages, 3 figures, accepted for publication in PR
Study of the impact of inhibitor on paraffin crystallization to rheological properties of crude oil
A monoclonal antibody raised against bacterially expressed MPV17 sequences shows peroxisomal, endosomal and lysosomal localisation in U2OS cells
Recessive mutations in the MPV17 gene cause mitochondrial DNA depletion syndrome, a fatal infantile genetic liver disease in humans. Loss of function in mice leads to glomerulosclerosis and sensineural deafness accompanied with mitochondrial DNA depletion. Mutations in the yeast homolog Sym1, and in the zebra fish homolog tra cause interesting, but not obviously related phenotypes, although the human gene can complement the yeast Sym1 mutation. The MPV17 protein is a hydrophobic membrane protein of 176 amino acids and unknown function. Initially localised in murine peroxisomes, it was later reported to be a mitochondrial inner membrane protein in humans and in yeast. To resolve this contradiction we tested two new mouse monoclonal antibodies directed against the human MPV17 protein in Western blots and immunohistochemistry on human U2OS cells. One of these monoclonal antibodies showed specific reactivity to a protein of 20 kD absent in MPV17 negative mouse cells. Immunofluorescence studies revealed colocalisation with peroxisomal, endosomal and lysosomal markers, but not with mitochondria. This data reveal a novel connection between a possible peroxisomal/endosomal/lysosomal function and mitochondrial DNA depletion