The Immune Response to Herpes Simplex Virus Type 1 Infection in Susceptible Mice is a Major Cause of CNS Pathology Resulting in Fatal Encephalitis

This study was undertaken to investigate possible immune mechanisms in fatal HSV-1 encephalitis (HSE) after HSV-1 corneal inoculation. Susceptible 129S6 (129) but not resistant C57BL/6 (B6) mice developed intense focal inflammatory brainstem lesions of primarily F4/80+ macrophages and Gr-1+ neutrophils detectable by MRI as early as day 6 post infection (PI). Depletion of macrophages and neutrophils significantly enhanced survival of infected 129 mice. Immunodeficient B6 (IL-7R-/-Kitw41/w41) mice lacking adaptive cells (B6-E mice) transplanted with 129 bone marrow showed significantly accelerated fatal HSE compared to B6-E mice transplanted with B6 marrow or control non-transplanted B6-E mice. In contrast, there was no difference in ocular viral shedding in B6-E mice transplanted with 129 bone marrow or B6 bone marrow. Acyclovir treatment of 129 mice beginning day 4 PI (24 h after HSV-1 first reaches the brain stem) reduced nervous system viral titers to undetectable levels but did not alter brainstem inflammation or mortality. We conclude that fatal HSE in 129 mice results from widespread damage in the brainstem caused by destructive inflammatory responses initiated early in infection by massive infiltration of innate cells

Inclusion of additional studies yields different conclusions: Comment on Sedikides, Gaertner, & Vevea (2005), Journal of Personality and Social Psychology

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75100/1/j.1467-839X.2007.00211.x.pd

Controle do ácaro da falsa ferrugem (Phyllocoptruta oleivora Ashm., 1879) por um juvenóide e outros defensivos agrícolas

In order to control the citrus rust mite on orange-trees a field experiment was carried out in Limeira, State of São Paulo, Brazil, an important citrus producing area. Treatments correspondend to a single application on 01/10/89 of: A) check; B) juvenoid flucycloxuron, 40 cm³; C) bromopropilate 65 cm³; D) quinomethionate + sulphur 200 cm³; E) quinomethionate + sulphur 250 cm³; F) fonnetanate 22,5 g; G) formetanate 31,5 g. The quantities are of commercial formulations in 100 liters of water. Each tree received 10 liters of the mixtures. Avaliations were made before spraying and 6, 20, 36, 50 and 68 days after the application. The most effective results in the last three avaliations were obtained with treatments F, G and C. Treatments D and E were effective only for 6 and 20 days and the juvenoid for 20, 36 and 50 days after spraying.Vários defensivos agrícolas, incluindo um juvenóide, foram aplicados contra o acaro da falsa ferrugem em pomar localizado em Limeira,SP, uma das principais áreas citrícolas do Brasil. Os tratamentos constaram de uma única aplicação, feita em 01/10/89. Tratamentos: A) testemunha; B) flucicloxurom (juvenóide) 40 cm³; C) bromopropilato 65 cm³; D) quinometionato + enxofre 200 cm³; E) quinometionato + enxofre 250 cm³; F) formetanato22,5 g; G) formetanato 31,5 g. As quantidades referem-se aos produtos comerciais para 100 litros de água. Cada laranjeira recebeu 10 litros de calda. Espalhante-adesivo: "Extravon" (25 cm³/100 litros). O delineamento estatístico feito foi de blocos ao acaso, com 4 repetições. Foram feitas 6 avaliações: 1 prévia e 5 após 06, 20, 36, 50 e 68 dias da aplicação. F, G e C foram os melhores tratamentos. D e E foram eficientes somente aos 06 e 20 dias e o juvenóide, aos 20, 36 e 50 dias

Ensaio de combate ao ácaro da leprose de citros Brevipalpus phoenicis (Geijskes, 1939) com novo juvenóide e outros acaricidas

A field test was carried out on adult orange trees sprayed with flucycloxuron, propargite and bromopropilate to check their efficiency in controlling leprosis mite. Treatments used were: A) check; B) flucycloxuron, 10g; C) flucycloxuron, 15g; D) flucycloxuron, 20g; E) flucycloxuron, 30g; F) propargite, 72g; G) bromopropilate, 37,5g. Quantities indicated are grams of active ingredients per 100 liters of water. Control evaluations were made 2 days before and 7, 20, 34 and 50 days after spraying. Treatments of propargite and bromopropilate turned out to be the most efficient. Treatments B, C, D, E were not efficient at all.Foi montado um ensaio visando conhecer a eficiência do flucicloxurom (juvenóide constituído de benzoil-fenil-uréia substituída), do propargite e do bromopropilato, no combate ao acaro da leprose (Brevipalpus phoenicis Geijskes, 1939). Os produtos foram empregados nas seguintes dosagens: A) testemunha; B) flucicloxurom, 10g; C) flucicloxurom, 15g; D) flucicloxurom, 20g; E) flucicloxurom, 30g; F) propargite, 72g; G) bromopropilato, 37,5g (tratamento padrão), sendo os valores supracitados quantidade de ingrediente ativo por 100 litros de calda. Foram aplicados 6 litros de calda por laranjeira, com pulverizador motorizado costal. Foram feitas 5 avaliações do combate: uma prévia (02 dias antes da pulverização) e quatro outras (07, 20, 34 e 50 dias pós-aplicação). A partir dos resultados obtidos, conclui-se que os tratamentos mais eficientes foram o propargite e o bromopropilato

Ácaro da leprose Bvevipalpus phoenicis (Geijskes, 1939): combate experimental em laranjeiras

In order to control the mite of leprosis B. phoeniois a field test was carried out in Piracicaba, State of São Paulo, Brazil, with the following treatments and active ingredients per 100 liters of water: A) check; B) clofentezine, 9.45g; C) clofentezine, 9.45g + multimethyl alkenols, 0.4g; D) quinomethionate, 25.0g; E) quinomethionate, 37.5g; F) pyrethroid RU-1000, 1.76g; G) pyrethroid RU-1000; 2.0g; H) dicofol, 37.0g (standard treatment). Each orange tree was sprayed with 6 liters of the product. Six evaluations were made: one 3 days before spraying and 5 post-treatment (5, 14, 18, 26 and 35 days after spraying. The best treatments were B, F, G and H.Com o objetivo de combater o ácaro da leprose foi montado um campo experimental com os seguintes tratamentos: A) testemunha; B) clofentezina, 9,45g; C) clofentezina, 9,45g + alquenóis multimetílicos, 0,4g; D) quinometionato, 25,Og; E) quinometionato, 37,5g; F) piretróide RU-1.000, l,76g; G) piretróide RU-1.0Q0, 2,0g; H) dicofol, 37,0g (tratamento padrão). As quantidades citadas são de ingredientes ativos em 1000 litros de calda (Tabela 1). Cada laranjeira foi pulverizada com 6 litros de calda, aplicada com pulverizador costal motorizado. Foram feitas 6 avaliações: a prévia (03 dias antes da pulverização) e 5 pos-aplicaçao (05, 14, 18, 26 e 35 dias após a aplicação). Pela análise dos resultados obtidos verifica-se que os tratamentos B, F, G e H. foram os mais eficientes. Os resultados da redução real podem ser observados na Tabela 2

Ácaro branco do algodão Polyphagotarsonemus latus (Banks, 1904) (Acari: Tarsonemidae) e ensaio de combate químico

Chemical control of the tropical mite was evaluated by means of a field test conducted in Tiete, State of São Paulo, Brazil. Seven treatments with four replicates each were applied to plots of 150m². Treatments and their respective active ingredients were as follows: A) check; B) triazophos, 300g; C) triazophos, 150g + pheromones (multimethyl alkenols), 4.77g; D) propargite, 1080g; E) abamectin, 6g + pheromones (multimethyl alkenols), 6.67g; F) profenophos, 400g; G) sulfur, 3000g. Best results were obtained with treatment B (triazophos), followed by G (sulfur) and the two mixtures with pheromones (C and E) (triazophos plus multimethyl alkenols and abamectin plus multimethyl alkenols) (tables 2, 3 and 4). Predador mites were considered.Com o intuito de avaliar o desempenho de produtos químicos no combate ao "ácaro branco" Polyphagotarsonemus latus (Banks, 1904) em algodoeiro, instalou-se o presente trabalho. Os 7 tratamentos, com 4 repetições, foram os seguintes: A) testemunha; B) triazofós, 300g; C) triazofós, 150g + feromônios (alquenóis multimetílicos), 4,77g; D) propargite, 1,080g; E) abamectina, 6g + feromônios (alquenóis multimetílicos), 6,67g; F) profenofós, 400g; G) enxofre, 3.000g. As quantidades acima são de ingredientes ativos por hectare. Os melhores resultados foram conseguidos pelo triazofós, seguido pelo enxofre e as duas misturas com feromônios

Pulverização experimental de defensivos químicos no combate ao ácaro da falsa ferrugem Phyllocoptruta oleivora (Ashm., 1879) (Acari: Eriophyidae) em citros

In order to control the citrus rust mite, adult orange-trees were submmited to the following treatments: A) check; B) pyrethroid S-604, 102g; C) S-604, 153g; D) S-604, 204g; E) juvenoid S-599, 22g; F) S-599, 44g; G) bromopropilate, 530g (standard treatment). Each tree was sprayed with 7.0 liters of liquid (water plus pesticide). Four evaluations were made: two days before spraying and 05, 19 and 40 days after the spraying. All treatments were efficient up to forty days after spraying. In the last evaluation the mite population had decreased to almost none.A fim de combater experimentalmente o ácaro da falsa ferrugem, pulverizaram-se laranjeiras adultas com os seguintes tratamentos e quantidades de ingrediente ativo por hectare: A) testemunha (nada aplicado); B) piretróide S-604 (102g); C) S-604(153g); D) S-604 (204g); E) juvenóide S-599 (22g); F) S-599 (44g); G) bromopropilato (530g) (tratamento padrão). Cada laranjeira recebeu 7,0 litros de calda em cobertura, aplicada com pulverizador costal motorizado. Realizaram-se 4 avaliações: a inicial (ou prévia) (02 dias antes da pulverização) e 3 pós-tratamento (05, 19 e 40 dias após a aplicação). Não foram possíveis novas avaliações devido à alta redução populacional do ácaro. Todos os tratamentos foram eficientes até 40 dias da pulverização. A Tabela 2 apresenta a relação de mortalidade real ou eficiência (%), a diferentes intervalos

EFEMP1 suppresses malignant glioma growth and exerts its action within the tumor extracellular compartment

<p>Abstract</p> <p>Purpose</p> <p>There are conflicting reports regarding the function of EFEMP1 in different cancer types. In this study, we sought to evaluate the role of EFEMP1 in malignant glioma biology.</p> <p>Experimental Design</p> <p>Real-time qRT-PCR was used to quantify <it>EFEMP1 </it>expression in 95 glioblastoma multiforme (GBM). Human high-grade glioma cell lines and primary cultures were engineered to express ectopic EFEMP1, a small hairpin RNA of EFEMP1, or treated with exogenous recombinant EFEMP1 protein. Following treatment, growth was assayed both <it>in vitro </it>and <it>in vivo </it>(subcutaneous (s.c.) and intracranial (i.c.) xenograft model systems).</p> <p>Results</p> <p>Cox regression revealed that EFEMP1 is a favorable prognostic marker for patients with GBM. Over-expression of EFEMP1 eliminated tumor development and suppressed angiogenesis, cell proliferation, and VEGFA expression, while the converse was true with knock-down of endogenous EFEMP1 expression. The EFEMP1 suppression of tumor onset time was nearly restored by ectopic VEGFA expression; however, overall tumor growth rate remained suppressed. This suggested that inhibition of angiogenesis was only partly responsible for EFEMP1's impact on glioma development. In glioma cells that were treated by exogenous EFEMP1 protein or over-expressed endogenous EFEMP1, the EGFR level was reduced and AKT signaling activity attenuated. Mixing of EFEMP1 protein with cells prior to s.c. and i.c. implantations or injection of the protein around the established s.c. xenografts, both significantly suppressed tumorigenicity.</p> <p>Conclusions</p> <p>Overall, our data reveals that EEFEMP1 suppresses glioma growth <it>in vivo</it>, both by modulating the tumor extracellular microenvironment and by altering critical intracellular oncogenic signaling pathways.</p