3,649 research outputs found
Fractionation of Hydrogen Isotopes by Sulfate- and Nitrate-Reducing Bacteria.
Hydrogen atoms from water and food are incorporated into biomass during cellular metabolism and biosynthesis, fractionating the isotopes of hydrogen-protium and deuterium-that are recorded in biomolecules. While these fractionations are often relatively constant in plants, large variations in the magnitude of fractionation are observed for many heterotrophic microbes utilizing different central metabolic pathways. The correlation between metabolism and lipid δ(2)H provides a potential basis for reconstructing environmental and ecological parameters, but the calibration dataset has thus far been limited mainly to aerobes. Here we report on the hydrogen isotopic fractionations of lipids produced by nitrate-respiring and sulfate-reducing bacteria. We observe only small differences in fractionation between oxygen- and nitrate-respiring growth conditions, with a typical pattern of variation between substrates that is broadly consistent with previously described trends. In contrast, fractionation by sulfate-reducing bacteria does not vary significantly between different substrates, even when autotrophic and heterotrophic growth conditions are compared. This result is in marked contrast to previously published observations and has significant implications for the interpretation of environmental hydrogen isotope data. We evaluate these trends in light of metabolic gene content of each strain, growth rate, and potential flux and reservoir-size effects of cellular hydrogen, but find no single variable that can account for the differences between nitrate- and sulfate-respiring bacteria. The emerging picture of bacterial hydrogen isotope fractionation is therefore more complex than the simple correspondence between δ(2)H and metabolic pathway previously understood from aerobes. Despite the complexity, the large signals and rich variability of observed lipid δ(2)H suggest much potential as an environmental recorder of metabolism
Apollo LM and CSM S-band antenna tracking studies. CSM-HGA interchangeability study error analysis of the monopulse comparators
Analysis of comparators used in CSM high gain antenna tracking syste
Kink stability, propagation, and length scale competition in the periodically modulated sine-Gordon equation
We have examined the dynamical behavior of the kink solutions of the
one-dimensional sine-Gordon equation in the presence of a spatially periodic
parametric perturbation. Our study clarifies and extends the currently
available knowledge on this and related nonlinear problems in four directions.
First, we present the results of a numerical simulation program which are not
compatible with the existence of a radiative threshold, predicted by earlier
calculations. Second, we carry out a perturbative calculation which helps
interpret those previous predictions, enabling us to understand in depth our
numerical results. Third, we apply the collective coordinate formalism to this
system and demonstrate numerically that it accurately reproduces the observed
kink dynamics. Fourth, we report on a novel occurrence of length scale
competition in this system and show how it can be understood by means of linear
stability analysis. Finally, we conclude by summarizing the general physical
framework that arises from our study.Comment: 19 pages, REVTeX 3.0, 24 figures available from A S o
The foreclosure crisis in 2008: predatory lending or household overreaching?
At least early in the financial crisis, the high rate of foreclosures seemed to be due more to households' overreaching than to predatory lending. A disproportionate number of those being foreclosed on were well-educated, well-off and relatively young people.Foreclosure ; Mortgage loans ; Housing - Finance
Lengthscales and Cooperativity in DNA Bubble Formation
It appears that thermally activated DNA bubbles of different sizes play
central roles in important genetic processes. Here we show that the probability
for the formation of such bubbles is regulated by the number of soft AT pairs
in specific regions with lengths which at physiological temperatures are of the
order of (but not equal to) the size of the bubble. The analysis is based on
the Peyrard- Bishop-Dauxois model, whose equilibrium statistical properties
have been accurately calculated here with a transfer integral approach
Ecologically and geologically relevant isotope signatures of C, N, and S: okenone producing purple sulfur bacteria part I
Purple sulfur bacteria (PSB) are known to couple the carbon, nitrogen, and sulfur cycling in euxinic environments. This is the first study with multiple strains and species of okenone-producing PSB to examine the carbon (C), nitrogen (N), and sulfur (S) metabolisms and isotopic signatures in controlled laboratory conditions, investigating what isotopic fractionations might be recorded in modern environments and the geologic record. PSB play an integral role in the ecology of euxinic environments and produce the unique molecular fossil okenane, derived from the diagenetic alteration of the carotenoid pigment okenone. Cultures of Marichromatium purpuratum 1591 (Mpurp1591) were observed to have carbon isotope fractionations (13_ε_biomass – CO_2), via RuBisCO, ranging from −16.1 to −23.2‰ during exponential and stationary phases of growth. Cultures of Thiocapsa marina 5653 (Tmar5653) and Mpurp1591 had a nitrogen isotope fractionation (15_ε_biomass – NH4) of −15‰, via glutamate dehydrogenase, measured and recorded for the first time in PSB. The δ^(34)S_VCDT values and amount of stored elemental sulfur for Mpurp1591 cells grown autotrophically and photoheterotrophically were dependent upon their carbon metabolic pathways. We show that PSB may contribute to the isotopic enrichments observed in modern and ancient anoxic basins. In a photoheterotrophic culture of Mpurp1591 that switched to autotrophy once the organic substrate was consumed, there were bulk biomass δ13C values that span a broader range than recorded across the Late Devonian, Permian–Triassic, Triassic–Jurassic, and OAE2 mass extinction boundaries. This finding stresses the complexities in interpreting and assigning δ13C values to bulk organic matter preserved in the geologic record
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