973 research outputs found
Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry
P pili are multisubunit fibers essential for the attachment of uropathogenic Escherichia coli to the kidney. These fibers are formed by the noncovalent assembly of six different homologous subunit types in an array that is strictly defined in terms of both the number and order of each subunit type. Assembly occurs through a mechanism termed âdonor-strand exchange (DSE)â in which an N-terminal extension (Nte) of one subunit donates a ÎČ-strand to an adjacent subunit, completing its Ig fold. Despite structural determination of the different subunits, the mechanism determining specificity of subunit ordering in pilus assembly remained unclear. Here, we have used noncovalent mass spectrometry to monitor DSE between all 30 possible pairs of P pilus subunits and their Ntes. We demonstrate a striking correlation between the natural order of subunits in pili and their ability to undergo DSE in vitro. The results reveal insights into the molecular mechanism by which subunit ordering during the assembly of this complex is achieved
Space Charge Study of the Jefferson Lab Magnetized Electron Beam
Magnetized electron cooling could result in high luminosity at the proposed Jefferson Lab Electron-Ion Collider (JLEIC). In order to increase the cooling efficiency, a bunched electron beam with high bunch charge and high repetition rate is required. We generated magnetized electron beams with high bunch charge using a new compact DC high voltage photo-gun biased at -300 kV with alkali-antimonide photocathode and a commercial ultrafast laser. This contribution explores how magnetization affects space charge dominated beams as a function of magnetic field strength, gun high voltage, laser pulse width, and laser spot size
Simulation Study of the Magnetized Electron Beam
Electron cooling of the ion beam plays an important role in electron ion colliders to obtain the required high luminosity. This cooling efficiency can be enhanced by using a magnetized electron beam, where the cooling process occurs inside a solenoid field. This paper compares the predictions of ASTRA and GPT simulations to measurements made using a DC high voltage photogun producing magnetized electron beam, related to beam size and rotation angles as a function of the photogun magnetizing solenoid and other parameters
Secondary structure of Ac-Ala-LysH polyalanine peptides (=5,10,15) in vacuo: Helical or not?
The polyalanine-based peptide series Ac-Ala_n-LysH+ (n=5-20) is a prime
example that a secondary structure motif which is well-known from the solution
phase (here: helices) can be formed in vacuo. We here revisit this conclusion
for n=5,10,15, using density-functional theory (van der Waals corrected
generalized gradient approximation), and gas-phase infrared vibrational
spectroscopy. For the longer molecules (n=10,15) \alpha-helical models provide
good qualitative agreement (theory vs. experiment) already in the harmonic
approximation. For n=5, the lowest energy conformer is not a simple helix, but
competes closely with \alpha-helical motifs at 300K. Close agreement between
infrared spectra from experiment and ab initio molecular dynamics (including
anharmonic effects) supports our findings.Comment: 4 pages, 4 figures, Submitted to JPC Letter
Evaluating Glucose Control With a Novel Composite Continuous Glucose Monitoring Index.
OBJECTIVE: The objective was to describe a novel composite continuous glucose monitoring index (COGI) and to evaluate its utility, in adults with type 1 diabetes, during hybrid closed-loop (HCL) therapy and multiple daily injections (MDI) therapy combined with real-time continuous glucose monitoring (CGM). METHODS: COGI consists of three key components of glucose control as assessed by CGM: Time in range (TIR), time below range (TBR), and glucose variability (GV) (weighted by 50%, 35% and 15%). COGI ranges from 0 to 100, where 1% increase of time 7.5-10%, had significantly higher COGI during 12 weeks of HCL compared to sensor-augmented pump therapy, mean (SD), 60.3 (8.6) versus 69.5 (6.9), P 7.5% to 9.9%, use of real-time CGM led to improved COGI, 49.8 (14.2) versus 58.2 (9.1), P < .0001. In MDI users with impaired awareness of hypoglycemia, use of real-time CGM led to improved COGI, 53.4 (12.2) versus 66.7 (11.1), P < .001. CONCLUSIONS: COGI summarizes three key aspects of CGM data into a concise metric that could be utilized to evaluate the quality of glucose control and to demonstrate the incremental benefit of a wide range of treatment modalities
Production of highly-polarized positrons using polarized electrons at MeV energies
The Polarized Electrons for Polarized Positrons experiment at the injector of
the Continuous Electron Beam Accelerator Facility has demonstrated for the
first time the efficient transfer of polarization from electrons to positrons
produced by the polarized bremsstrahlung radiation induced by a polarized
electron beam in a high- target. Positron polarization up to 82\% have been
measured for an initial electron beam momentum of 8.19~MeV/, limited only by
the electron beam polarization. This technique extends polarized positron
capabilities from GeV to MeV electron beams, and opens access to polarized
positron beam physics to a wide community.Comment: 5 pages, 4 figure
Simulation Study of the Emittance Measurements in Magnetized Electron Beam
Electron cooling of the ion beam is key to obtaining the required high luminosity of proposed electron-ion colliders. For the Jefferson Lab Electron Ion Collider, the expected luminosity of 10³⎠ă cmăâ»ÂČ sâ»Âč will be achieved through so-called âmagnetized electron coolingâ, where the cooling process occurs inside a solenoid field, which will be part of the collider ring and facilitated using a circulator ring and Energy Recovery Linac (ERL). As an initial step, we generated magnetized electron beam using a new compact DC high voltage photogun biased at -300 kV employing an alkali-antimonide photocathode. This contribution presents the characterization of the magnetized electron beam (emittance variations with the magnetic field strength for different laser spot sizes) and a comparison to GPT simulations
Precision Electron-Beam Polarimetry using Compton Scattering at 1 GeV
We report on the highest precision yet achieved in the measurement of the
polarization of a low energy, (1 GeV), electron beam, accomplished
using a new polarimeter based on electron-photon scattering, in Hall~C at
Jefferson Lab. A number of technical innovations were necessary, including a
novel method for precise control of the laser polarization in a cavity and a
novel diamond micro-strip detector which was able to capture most of the
spectrum of scattered electrons. The data analysis technique exploited track
finding, the high granularity of the detector and its large acceptance. The
polarization of the A, ~GeV electron beam was measured with a
statistical precision of ~1\% per hour and a systematic uncertainty of
0.59\%. This exceeds the level of precision required by the \qweak experiment,
a measurement of the vector weak charge of the proton. Proposed future
low-energy experiments require polarization uncertainty ~0.4\%, and this
result represents an important demonstration of that possibility. This
measurement is also the first use of diamond detectors for particle tracking in
an experiment.Comment: 9 pages, 7 figures, published in PR
Variability of Insulin Requirements Over 12 Weeks of Closed-Loop Insulin Delivery in Adults With Type 1 Diabetes.
OBJECTIVE: To quantify variability of insulin requirements during closed-loop insulin delivery. RESEARCH DESIGN AND METHODS: We retrospectively analyzed overnight, daytime, and total daily insulin amounts delivered during a multicenter closed-loop trial involving 32 adults with type 1 diabetes. Participants applied hybrid day-and-night closed-loop insulin delivery under free-living home conditions over 12 weeks. The coefficient of variation was adopted to measure variability of insulin requirements in individual subjects. RESULTS: Data were analyzed from 1,918 nights, 1,883 daytime periods and 1,564 total days characterized by closed-loop use over 85% of time. Variability of overnight insulin requirements (mean [SD] coefficient of variation 31% [4]) was nearly twice as high as variability of total daily requirements (17% [3], P < 0.001) and was also higher than variability of daytime insulin requirements (22% [4], P < 0.001). CONCLUSIONS: Overnight insulin requirements were significantly more variable than daytime and total daily amounts. This may explain why some people with type 1 diabetes report frustrating variability in morning glycemia.Seventh Framework Programme of the European Union (ICT FP7- 247138). Additional support for the Artificial Pancreas work by JDRF, National Institute for Health Research Cambridge Biomedical Research Centre and Wellcome Strategic Award (100574/Z/12/Z). Abbott Diabetes Care supplied discounted continuous glucose monitoring devices, sensors, and communication protocol to facilitate real-time connectivity.
We acknowledge support by the staff at the Addenbrookeâs Wellcome Trust Clinical Research Facility.
Jasdip Mangat and John Lum (Jaeb Center) supported development and validation of the closed-loop system. Josephine Hayes (University of Cambridge) provided administrative support. Karen Whitehead (University of Cambridge) provided laboratory support. We acknowledge support by the staff at Profil Institut; Krisztina Schmitz-Grozs provided support as a research physician, Martina Haase supported the study as an insulin pump expert, and Maren Luebkert, Kirstin Kuschma and Elke Przetak provided administrative, coordinating and documentation support.This is the author accepted manuscript. The final version is available from the American Diabetes Association via http://dx.doi.org/10.2337/dc15-262
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