Glucocorticoid-regulated localization of cell surface glycoproteins in rat hepatoma cells is mediated within the Golgi complex.

Glucocorticoid hormones regulate the post-translational maturation and sorting of cell surface and extracellular mouse mammary tumor virus (MMTV) glycoproteins in M1.54 cells, a stably infected rat hepatoma cell line. Exposure to monensin significantly reduced the proteolytic maturation and externalization of viral glycoproteins resulting in a stable cellular accumulation of a single 70,000-Mr glycosylated polyprotein (designated gp70). Cell surface- and intracellular-specific immunoprecipitations of monensin-treated cells revealed that gp70 can be localized to the cell surface only in the presence of 1 microM dexamethasone, while in uninduced cells gp70 is irreversibly sequestered in an intracellular compartment. Analysis of oligosaccharide processing kinetics demonstrated that gp70 acquired resistance to endoglycosidase H with a half-time of 65 min in the presence or absence of hormone. In contrast, gp70 was inefficiently galactosylated after a 60-min lag in uninduced cells while rapidly acquiring this carbohydrate modification in the presence of dexamethasone. Furthermore, in the absence or presence of monensin, MMTV glycoproteins failed to be galactosylated in hormone-induced CR4 cells, a complement-selected sorting variant defective in the glucocorticoid-regulated compartmentalization of viral glycoproteins to the cell surface. Since dexamethasone had no apparent global effects on organelle morphology or production of total cell surface-galactosylated species, we conclude that glucocorticoids induce the localization of cell surface MMTV glycoproteins by regulating a highly selective step within the Golgi apparatus after the acquisition of endoglycosidase H-resistant oligosaccharide side chains but before or at the site of galactose attachment

Gravitational Settling of ^{22}Ne in Liquid White Dwarf Interiors--Cooling and Seismological Effects

We assess the impact of the trace element ^{22}Ne on the cooling and seismology of a liquid C/O white dwarf (WD). Due to this elements' neutron excess, it sinks towards the interior as the liquid WD cools. The subsequent gravitational energy released slows the cooling of the WD by 0.25--1.6 Gyrs by the time it has completely crystallized, depending on the WD mass and the adopted sedimentation rate. The effects will make massive WDs or those in metal rich clusters (such as NGC 6791) appear younger than their true age. Our diffusion calculations show that the ^{22}Ne mass fraction in the crystallized core actually increases outwards. The stability of this configuration has not yet been determined. In the liquid state, the settled ^{22}Ne enhances the internal buoyancy of the interior and changes the periods of the high radial order g-modes by approximately 1%. Though a small adjustment, this level of change far exceeds the accuracy of the period measurements. A full assessment and comparison of mode frequencies for specific WDs should help constrain the still uncertain ^{22}Ne diffusion coefficient for the liquid interior.Comment: 26 pages (11 text pages with 15 figures); to appear in The Astrophysical Journa

Gravitational settling of 22Ne and white dwarf evolution

We study the effects of the sedimentation of the trace element 22Ne in the cooling of white dwarfs. In contrast with previous studies, which adopted a simplified treatment of the effects of 22Ne sedimentation, this is done self-consistently for the first time, using an up-to-date stellar evolutionary code in which the diffusion equation is coupled with the full set of equations of stellar evolution. Due the large neutron excess of 22Ne, this isotope rapidly sediments in the interior of the white dwarf. Although we explore a wide range of parameters, we find that using the most reasonable assumptions concerning the diffusion coefficient and the physical state of the white dwarf interior the delay introduced by the ensuing chemical differentation is minor for a typical 0.6 Msun white dwarf. For more massive white dwarfs, say M_Wd about 1.0 Msun, the delay turns out to be considerably larger. These results are in qualitatively good accord with those obtained in previous studies, but we find that the magnitude of the delay introduced by 22Ne sedimentation was underestimated by a factor of about 2. We also perform a preliminary study of the impact of 22Ne sedimentation on the white dwarf luminosity function. Finally, we hypothesize as well on the possibility of detecting the sedimentation of 22Ne using pulsating white dwarfs in the appropriate effective temperature range with accurately determined rates of change of the observed periods.Comment: To apper in The Astrophysical Journa

Abdominal obesity in adolescents: Development of age-specific waist circumference cut-offs linked to adult IDF criteria

Objectives: The purpose of this study was to develop age- and sex-specific waist cir-cumference (WC) cut-off points, linked to older adolescent and adult criteria forabdominal obesity, to be applied to children in the clinical setting.Methods: A total of 16,788 adolescents aged 10 to 16 years were assessed for WC.Smoothed age and sex-specific WC curves were obtained using Cole’s LMS method.Results: Percentiles that corresponded to the International Diabetes Federation (IDF)recommendations used for older adolescents and adults (161 years old) were the97thpercentile for boys and the 87thpercentile for girls. Using these cutoffs, a total of368 boys and 1138 girls were categorized as abdominally obese, in contrast to 1654boys and 987 girls that were identified using the current IDF pediatric criteria (90thpercentile).Conclusions: We provide age- and sex-specific cut-off points that can be used toidentify abdominal obesity in adolescents. The present findings provide a tool thatcan be used in the clinical setting for the early detection and prevention of adult obe-sity. Population-specific cutoffs may be required for pediatric ages to diagnosechildren at risk

Axisymmetric smoothed particle hydrodynamics with self-gravity

The axisymmetric form of the hydrodynamic equations within the smoothed particle hydrodynamics (SPH) formalism is presented and checked using idealized scenarios taken from astrophysics (free fall collapse, implosion and further pulsation of a sun-like star), gas dynamics (wall heating problem, collision of two streams of gas) and inertial confinement fusion (ICF, -ablative implosion of a small capsule-). New material concerning the standard SPH formalism is given. That includes the numerical handling of those mass points which move close to the singularity axis, more accurate expressions for the artificial viscosity and the heat conduction term and an easy way to incorporate self-gravity in the simulations. The algorithm developed to compute gravity does not rely in any sort of grid, leading to a numerical scheme totally compatible with the lagrangian nature of the SPH equations.Comment: 17 pages, 10 figures, 1 Table. Accepted for publication in MNRA

Swope Supernova Survey 2017a (SSS17a), the Optical Counterpart to a Gravitational Wave Source

On 2017 August 17, the Laser Interferometer Gravitational-wave Observatory (LIGO) and the Virgo interferometer detected gravitational waves emanating from a binary neutron star merger, GW170817. Nearly simultaneously, the Fermi and INTEGRAL telescopes detected a gamma-ray transient, GRB 170817A. 10.9 hours after the gravitational wave trigger, we discovered a transient and fading optical source, Swope Supernova Survey 2017a (SSS17a), coincident with GW170817. SSS17a is located in NGC 4993, an S0 galaxy at a distance of 40 megaparsecs. The precise location of GW170817 provides an opportunity to probe the nature of these cataclysmic events by combining electromagnetic and gravitational-wave observations.Comment: 25 pages, 10 figures, 2 tables, published today in Scienc

Constraining deflagration models of Type Ia supernovae through intermediate-mass elements

The physical structure of a nuclear flame is a basic ingredient of the theory of Type Ia supernovae (SNIa). Assuming an exponential density reduction with several characteristic times we have followed the evolution of a planar nuclear flame in an expanding background from an initial density 6.6 10^7 g/cm3 down to 2 10^6 g/cm3. The total amount of synthesized intermediate-mass elements (IME), from silicon to calcium, was monitored during the calculation. We have made use of the computed mass fractions, X_IME, of these elements to give an estimation of the total amount of IME synthesized during the deflagration of a massive white dwarf. Using X_IME and adopting the usual hypothesis that turbulence decouples the effective burning velocity from the laminar flame speed, so that the relevant flame speed is actually the turbulent speed on the integral length-scale, we have built a simple geometrical approach to model the region where IME are thought to be produced. It turns out that a healthy production of IME involves the combination of not too short expansion times, t_c > 0.2 s, and high turbulent intensities. According to our results it could be difficult to produce much more than 0.2 solar masses of intermediate-mass elements within the deflagrative paradigma. The calculations also suggest that the mass of IME scales with the mass of Fe-peak elements, making it difficult to conciliate energetic explosions with low ejected nickel masses, as in the well observed SN1991bg or in SN1998de. Thus a large production of Si-peak elements, especially in combination with a low or a moderate production of iron, could be better addressed by either the delayed detonation route in standard Chandrasekhar-mass models or, perhaps, by the off-center helium detonation in the sub Chandrasekhar-mass scenario.Comment: 9 pages, 5 figures, 2 table

Photonic crystal optical waveguides for on-chip Bose-Einstein condensates

We propose an on-chip optical waveguide for Bose-Einstein condensates based on the evanescent light fields created by surface states of a photonic crystal. It is shown that the modal properties of these surface states can be tailored to confine the condensate at distances from the chip surface significantly longer that those that can be reached by using conventional index-contrast guidance. We numerically demonstrate that by index-guiding the surface states through two parallel waveguides, the atomic cloud can be confined in a two-dimensional trap at about 1$\mu$m above the structure using a power of 0.1mW.Comment: 5 pages, 4 figure

The Old Host-Galaxy Environment of SSS17a, the First Electromagnetic Counterpart to a Gravitational Wave Source

We present an analysis of the host-galaxy environment of Swope Supernova Survey 2017a (SSS17a), the discovery of an electromagnetic counterpart to a gravitational wave source, GW170817. SSS17a occurred 1.9 kpc (in projection; 10.2") from the nucleus of NGC 4993, an S0 galaxy at a distance of 40 Mpc. We present a Hubble Space Telescope (HST) pre-trigger image of NGC 4993, Magellan optical spectroscopy of the nucleus of NGC 4993 and the location of SSS17a, and broad-band UV through IR photometry of NGC 4993. The spectrum and broad-band spectral-energy distribution indicate that NGC 4993 has a stellar mass of log (M/M_solar) = 10.49^{+0.08}_{-0.20} and star formation rate of 0.003 M_solar/yr, and the progenitor system of SSS17a likely had an age of >2.8 Gyr. There is no counterpart at the position of SSS17a in the HST pre-trigger image, indicating that the progenitor system had an absolute magnitude M_V > -5.8 mag. We detect dust lanes extending out to almost the position of SSS17a and >100 likely globular clusters associated with NGC 4993. The offset of SSS17a is similar to many short gamma-ray burst offsets, and its progenitor system was likely bound to NGC 4993. The environment of SSS17a is consistent with an old progenitor system such as a binary neutron star system.Comment: ApJL in pres