Relationships between exercise and three components of mental well-being in corporate employees

Objectives: The main purpose of the present study was to examine the relationships between exercise participation and three components of mental well-being (physical self, work-related, and global) in a sample of corporate employees. As a subsidiary and exploratory question, we also examined whether these well-being components are more strongly related to structured exercise participation scores compared to total levels of physical activity. Design: Cross-sectional survey. Method: The participants were 312 employees from an information technology company (nZ204 males and nZ108 females). Structural equation modelling was used to examine links between exercise participation and the three well-being components within a hierarchical framework, featuring global well-being constructs at the apex and specific elements of well-being at lower levels. Results: Support was found for the a priori model in that there were direct paths from exercise to physical self and enthusiasm at work. Furthermore, there were indirect paths between exercise and global well-being components through measures of the physical self and enthusiasm at work. The results of an alternative model using physical activity as opposed to exercise were generally similar. Conclusion: The support found for the exercise and well-being model indicates that exercise is associated directly and indirectly with high well-being in various facets of employees’ lives. Thus, this study extends previous research that has examined associations between exercise and isolated indicators of employee well-being. Finally, the results pertaining to physical activity suggest that workplace exercise promotion programmes should incorporate and promote lifestyle physical activity

Balancing life: perceptions and practices of health among young adult Yup'ik women

Thesis (M.A.) University of Alaska Fairbanks, 2014.Ten years ago CANHR (Center for Alaska Native Health Research) asked Yup'ik men and women of all ages how they would define health and wellness; that is, what it means to be well and happy. The answers were largely centered on living a subsistence lifestyle, eating subsistence foods and respecting natural spirits and lands. Today a new generation of young Yup'ik women has emerged. A generation that has grown up in villages and cities with storebought food available next to subsistence food, TV, and Internet. In this study young adult Yup'ik women's perceptions of health and their use of dancing as a practice of health are investigated. This study looks at how this new generation of young adult Yup'ik women understand health. Young adult Yup'ik women's perceptions and practices of health, such as dancing, are examined to determine what these women consider important to stay healthy and how the notion of health itself can be understood. Ideas of what it means to the subjects comprising the study population to be healthy are crucial to understand before conducting any kind of health research. How people interpret, navigate and understand the very notion of health must be uncovered in order to work with them on any and all health issues. As such the notion of health cannot and should not be conceptionalized as the mere presence or absence of disease but includes instead a wide network of social, spiritual, physical, mental, and emotional factors. Consequently, this study approaches health from a holistic perspective implementing a wide network of factors in the investigation of young, adult, Yup'ik women's perceptions and practices of health.Chapter 1. Introduction -- 1.1. Motivation for and significance of studying Yup'ik women's health -- 1.2. Epistemological considerations -- 1.3. Defining central research terms -- 1.3.1. Young adult -- 1.3.2. Yup'ik -- 1.3.3. Women -- 1.3.4. Research Partners -- 1.3.5. Health -- 1.3.6. Traditional/contemporary (dichotomies) -- Chapter 2. Methodology -- 2.1. Research settings: the field -- 2.2. The process of establishing contact -- 2.3. Interviews -- 2.4. Participant observation -- 2.5. Ethical considerations -- 2.6. My Yup'ik research partners -- 2.6.1. Hannah -- 2.6.2. Julia -- 2.6.3. Sarah -- 2.6.4. Bonita -- 2.6.5. Ruth -- 2.6.6. William -- 2.6.7. Theresa Arevgaq John -- 2.6.8. Inu-Yupiaq dance group -- 2.7. Perceptions and practices -- 2.8. Data analysis -- 2.9. Health in the Arctic: major topics and where this study fits in -- 2.10. Yup'ik people: culture, health and change -- 2.11. Urban Yup'ik -- Chapter 3. Analysis I: Perceptions and practices of health -- 3.1. Being balanced -- 3.2. Yup'ik cultural identity -- 3.3. Exercise -- 3.4. Food -- 3.5. Stress -- 3.6. Substance use -- 3.7. Spirituality -- 3.8. Community -- 3.9. Health: developing the notion -- Chapter 4. Analysis II: Dancing as a Practice of Health -- 4.1. Dancing as a way to maintain balance -- 4.2. Yup'ik cultural identity in dancing -- 4.3. Dance as exercise -- 4.4. Food in and around dancing -- 4.5. Dancing as stress relief -- 4.6. Dancing as a break from substance use -- 4.7. Spirituality in dancing -- 4.8. Community of dancers -- 4.9. The notion of health: dancing -- Chapter 5. Conclusion -- Bibliography -- Appendices

Integrated presentation of ecological risk from multiple stressors

Current environmental risk assessments (ERA) do not account explicitly for ecological factors (e.g. species composition, temperature or food availability) and multiple stressors. Assessing mixtures of chemical and ecological stressors is needed as well as accounting for variability in environmental conditions and uncertainty of data and models. Here we propose a novel probabilistic ERA framework to overcome these limitations, which focusses on visualising assessment outcomes by construct-ing and interpreting prevalence plots as a quantitative prediction of risk. Key components include environmental scenarios that integrate exposure and ecology, and ecological modelling of relevant endpoints to assess the effect of a combination of stressors. Our illustrative results demonstrate the importance of regional differences in environmental conditions and the confounding interactions of stressors. Using this framework and prevalence plots provides a risk-based approach that combines risk assessment and risk management in a meaningful way and presents a truly mechanistic alternative to the threshold approach. Even whilst research continues to improve the underlying models and data, regulators and decision makers can already use the framework and prevalence plots. The integration of multiple stressors, environmental conditions and variability makes ERA more relevant and realistic

Painting the Palace of Apries I: ancient binding media and coatings of the reliefs from the Palace of Apries, Lower Egypt

This study gives an account of the organic components (binders and coatings) found in the polychromy of some fragmented architectural reliefs from the Palace of Apries in Memphis, Egypt (26th Dynasty, ca. 589-568 BCE). A column capital and five relief fragments from the collections of the Ny Carlsberg Glyptotek in Copenhagen were chosen for examination, selected because of their well-preserved polychromy. Samples from the fragments were first investigated using Fourier transform infrared (FTIR) spectroscopy to screen for the presence of organic materials and to identify the chemical family to which these materials belong (proteinaceous, polysaccharides or lipid). Only the samples showing the potential presence of organic binder residues were further investigated using gas chromatography with mass spectrometry detection (GC-MS) targeting the analysis towards the detection and identification of compounds belonging to the chemical families identified by FTIR. The detection of polysaccharides in the paint layers on the capital and on two of the fragments indicates the use of plant gums as binding media. The interpretation of the sugar profiles was not straightforward so botanical classification was only possible for one fragment where the results of analysis seem to point to gum arabic. The sample from the same fragment was found to contain animal glue and a second protein material (possibly egg). While the presence of animal glue is probably ascribable to the binder used for the ground layer, the second protein indicates that either the paint layer was bound in a mixture of different binding materials or that the paint layer, bound in a plant gum, was then coated with a proteinaceous material. The surface of two of the investigated samples was partially covered by translucent waxy materials that were identified as a synthetic wax (applied during old conservation treatments) and as beeswax, respectively. It is possible that the beeswax is of ancient origin, selectively applied on yellow areas in order to create a certain glossiness or highlight specific elements

Germline PTEN mutations are rare and highly penetrant

Cowden syndrome (multiple hamartoma syndrome, MIM 158350) is an early onset syndrome characterized by multiple hamartomas in the skin, mucous membranes, breast, thyroid and endometrium. Patients with Cowden syndrome have increased risk of breast cancer, thyroid cancer and endometrial cancer. In 1997 germline mutations in PTEN were demonstrated to cause Cowden syndrome. We report the results of diagnostic and predictive testing in all families with Cowden syndrome or suspected Cowden syndrome registered at the Norwegian cancer family clinics. PTEN mutations were found in all six families meeting the clinical criteria for Cowden syndrome, in none of the two families assumed to have Cowden syndrome but not fulfilling the criteria, and in none of the eight families selected in our computerized medical files to have a combination of breast and thyroid cancers. Age-related penetrances for the various neoplasms are given. All families but one were small and de novo mutations were found

Stress, Physical Activity, and Resilience Resources: Tests of Direct and Moderation Effects in Young Adults

Stress is an important consideration for understanding why individuals take part in limited or no physical activity (PA). The effects of stress on PA do not hold for everyone, so examinations of possible moderators that protect individuals from the harmful effects of stress are required. Aligned with a resilience framework, individual resources (e.g., hope and self-efficacy) may buffer the maladaptive effects of stress, such that people who have access to these resources in greater quantity may be more "resilient" to the deleterious effects of stress on PA. This study was designed to test this expectation. In total, 140 Australian undergraduate students (70.7% female, Mage = 21.68 ± 4.88) completed a multisection survey and provided a sample for hair cortisol concentration analysis using immunoassays. Main effects demonstrated primarily small and nonsignificant associations between perceived stress and hair cortisol concentration with different intensities of PA. Similar findings were observed between individual-level resilience resources and PA intensities, with the exception of hope (i.e., positive association with vigorous PA and negative association with sitting), self-efficacy (i.e., positive association with vigorous PA), and resilience (i.e., positive association with walking). Although certain individual-level resilience resources were perceived as beneficial for PA and sedentary time, the moderating role of resilience resources was not supported by the findings. The direct and moderating effects between stress, PA, and resilience resources require further testing using longitudinal designs in which stressful periods occur naturally (e.g., exams for students) or are experimentally manipulated

"Step by Step". A feasibility study of a lunchtime walking intervention designed to increase walking, improve mental well-being and work performance in sedentary employees: Rationale and study design

BACKGROUND: Following an extensive recruitment campaign, a 16-week lunchtime intervention to increase walking was implemented with insufficiently physically active University employees to examine programme feasibility and the effects of the programme in increasing walking behaviour, and in improving well-being and work performance. METHODS/DESIGN: A feasibility study in which participants were randomised to an immediate treatment or a delayed treatment control (to start at 10 weeks) group. For the first ten weeks of the intervention, participants took part in three facilitator-led group walks per week each of thirty minutes duration and were challenged to accumulate another sixty minutes of walking during the weekends. In the second phase of the intervention, the organised group walks ceased to be offered and participants were encouraged to self-organise their walks. Motivational principles were employed using contemporary motivational theory. Outcome measures (including self-reported walking, step counts, cardiovascular fitness, general and work-related well-being and work performance) were assessed at baseline, at the end of the 16-week intervention and (for some) four months after the end of the intervention. Process and outcome assessments were also taken throughout, and following, the intervention. DISCUSSION: The results of the intervention will determine the feasibility of implementing a lunchtime walking programme to increase walking behaviour, well-being and performance in sedentary employees. If successful, there is scope to implement definitive trials across a range of worksites with the aim of improving both employee and organisational health

Comparison of RNAi efficiency mediated by tetracycline-responsive H1 and U6 promoter variants in mammalian cell lines

Conditional expression of short hairpin RNAs (shRNAs) to knock down target genes is a powerful tool to study gene function. The most common inducible expression systems are based on tetracycline-regulated RNA polymerase III promoters. During the last years, several tetracycline-inducible U6 and H1 promoter variants have been reported in different experimental settings showing variable efficiencies. In this study, we compare the most common variants of these promoters in several mammalian cell lines. For all cell lines tested, we find that several inducible U6 and H1 promoters containing single tetracycline operator (tetO) sequences show high-transcriptional background in the non-induced state. Promoter variants containing two tetO sequences show tight suppression of transcription in the non-induced state, and high tet responsiveness and high gene knockdown efficiency upon induction in all cell lines tested. We report a variant of the H1 promoter containing two O2-type tetO sequences flanking the TATA box that shows little transcriptional background in the non-induced state and up to 90% target knockdown when the inducer molecule (dox–doxycycline) is added. This inducible system for RNAi-based gene silencing is a good candidate for use both in basic research on gene function and for potential therapeutic applications

Novel snakebite therapeutics must be tested in appropriate rescue models to robustly assess their preclinical efficacy

In the field of antivenom research, development, and manufacture, it is often advised to follow the World Health Organization’s (WHO) guidelines for the production, control, and regulation of snake antivenom immunoglobulins, which recommend the use of preincubation assays to assess the efficacy of snakebite therapeutics. In these assays, venom and antivenom are mixed and incubated prior to in vivo administration to rodents, which allows for a standardizable comparison of antivenoms with similar characteristics. However, these assays are not necessarily sufficient for therapeutics with significantly different pharmacological properties than antibody-based antivenoms, such as small molecule inhibitors, nanoparticles, and other modalities. To ensure that the in vivo therapeutic utility of completely novel toxin-neutralizing molecules with no history of use in envenoming therapy and variable pharmacokinetics is properly evaluated, such molecules must also be tested in preclinical rescue assays, where rodents are first challenged with appropriate doses of venoms or toxins, followed by the administration of neutralizing modalities after an appropriate time delay to better mimic the real-life scenarios faced by human snakebite victims. Such an approach takes the venom (or toxin) toxicokinetics, the drug pharmacokinetics, and the drug pharmacodynamics into consideration. If new modalities are only assessed in preincubation assays and not subjected to evaluation in rescue assays, the publication of neutralization data may unintentionally misrepresent the actual therapeutic efficacy and suitability of the modality being tested, and thus potentially misguide strategic decision making in the research and development of novel therapies for snakebite envenomingVillum Foundation/[Project 00025302]//DinamarcaBioPorto Diagnostics A/S/[]//DinamarcaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP