Inducible Protective Processes in Animal Systems .5. Clastogenic Adaptation Triggered by Low-Dose of Methyl Methanesulfonate (Mms) in Poecilocerus-Pictus

The monofunctional alkylating agent methyl methanesulfonate (MMS) was used to study the induction of inducible protective processes (clastogenic adaptation) in meiotic cells of the grasshopper, Poecilocerus pictus. After a low (conditioning) dose of 0.012 M and challenging with a high (challenging) dose of 0.048 M MMS after 2 h time-lag, the incidence of chromosomal anomalies in metaphase I, II and anaphase I and II stages was found to be significantly less (P < 0.05) compared to that of the additive effect of both the conditioning and the challenging doses. The data suggest that the low dose of MMS rendered the meiotic cells more resistant to the clastogenic effects of a subsequent high dose of MMS employed. These results lend further support to the earlier report from the same laboratory where a low dose of ethyl methanesulfonate (EMS) was found to render protection against a high dose of the same clastogen in meiotic cells of P. pictus. Furthermore, it is clear from the results that conditioning by the methylating (MMS) agent in cells is less effective in comparison with that of the ethylating (EMS) agent

Home Ownership, Savings, and Mobility Over The Life Cycle

In a Bewley model with endogenous price volatility, home ownership and mobility across locations and jobs, we assess the contribution of financial constraints, housing illiquidities and house price risk to home ownership over the life cycle. The model can explain the rise in home ownership and fall in mobility over the life cycle. While some households rent due to borrowing constraints in the mortgage market, factors that effect propensities to save and move, such as risky house values and transactions costs, are more important determinants of the ownership rate

Impact of chromophores on colour appearance in a computational skin model

Early diagnosis of skin cancer offers the patient more favorable treatment options. Color fidelity of skin images is a major concern for dermatologists as adoption of digital dermatoscopes is increasing rapidly. Accurate color depiction of the lesion and surrounding skin are vital in diagnostic evaluation of a lesion. We previously introduced VCT-Derma, a pipeline for dermatological Virtual Clinical Trials (VCTs) including detailed and flexible models of human skin and lesions, which represent the patient in the entire dermatoscopy-based diagnostic process. However, those initial models of skin and lesions did not properly account for tissue colors. Our new skin model accounts for tissue color appearance by incorporating chromophores (e.g., melanin, blood) into the tissue model, and simulating the optical properties of the various skin layers. The physical properties of the skin and lesion were selected from clinically plausible values. The model and simulated dermatoscope images were created in open modelling software, assuming a linear camera model. We have assumed ambient white lighting, with a 6mm distance to the camera. Our model of color appearance was characterised by comparing the brightness of the lesion to its depth. The brightness of the lesion is compared through the variability of the mean gray values of a cropped region around the lesion. We compare two skin models, one without extensive chromophore content and one with. Our preliminary evaluation of increasing chromophore content shows promise based on the results presented here. Further refinement and validation of the model is ongoing

Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes

Human peripheral blood lymphocytes stimulated in vitro for 6 hr were exposed to a low (conditioning) dose of ethyl methanesulfonate (EMS; 1.5 x 10(-4) M) or methyl methanesulfonate (MMS; 1.5 x 10(-5) M). After 6 hr, the cells were treated with a high (challenging) concentration of the same agent (1.5 x 10(-3) M EMS or 1.5 x 10(-4) M MMS). The cells that received both conditioning and challenging doses became less sensitive to the induction of sister chromatid exchanges (SCEs) than those which did not receive the pretreatment with EMS or MMS. They responded with lower frequencies of SCEs. This suggests that conditioning dose of EMS or MMS has offered the lymphocytes to have decreased SCEs. This led to the realization that pre-exposure of lymphocytes to low dose can cause the induction of repair activity. This is a clear indication of the existence of adaptive response induced by alkylating agents whether it is ethylating or methylating in human lymphocytes in vitro

Effect of sintering and coldbonding techniques onlightweight aggregates fromindustrial waste – a review

Concrete is a building material which consist major portion of hard inorganic materials called aggregates such as crushed stone aggregate. Due to continuous usage of natural resources within a short length of time these get depleted. Production of concreteby using waste materials from industries is important in this era. The production of light weight aggregate from waste material is done by pellatization and hardened by sintering or cold bonding techniques. Depending upon the hardening methods the properties of concrete is also varied. From previous research works it can be concluded that it is possible to produce structural concrete using sintered fly ash aggregate which are spherical in shape having specific gravity varying from 1.33 to 2.35. According to the literature, the specific gravity of these aggregates was 16 to 46 percent lower than that of standard of weight aggregates, and they might be employed as aggregates in structural concrete production depending on the constituent qualities and the production environment. Cold bonded aggregate concretewith compressive strength 48% lesser compared to normal concrete mix, it exceeds the value of 17 MPa that has been fixed as minimum criteria for concrete to be used as a structural material. This paper mainly focuses on review of artificial light weight aggregate from cold bonding and sintering methods and how these methods affect the concrete properties. From different studies, it is concluded that Cold bonding is an energy saving method and Sintered sludge pellets have a stronger aggregate strength, larger porosity, and lower aggregate density than cold bonded aggregates, exhibiting qualities that are superior to those required of building aggregates. Sustainable development is indeed achievable in this developing era and lightweight aggregate in construction sector is key to achieve this

A review on the effects of artificial light weight aggregate in concrete

The disposal problem of industrial by-products like fly ash, heavy metal sludge, sewage sludge etc. are increasing day by day. To use by-products in large volume the applications like embankment fill or aggregate replacement material should be considered for sustainable development. This study is focused on properties of artificial light weight aggregate on concrete and the effect of cold bonded light weight aggregate on concrete through partial and complete replacement of coarse aggregates. Artificial Lightweight aggregate can be produced by nodulizing the by-product for example fly ash in a pelletizer with a proportionate quantity of water, cement and further hardened by cold bonding or sintering. Due to the impact of earth quake forces all over the world, the need for light weight structural design is increasing presently, as it reduces mass of the structure. The concrete produced is light weight in nature and has added the benefit of reducing overall cost, especially in transportation and placing etc. it has its own advantages like reduced dead load, and thus economic structures, high sound absorption and good fire resistance. Keywords—Artificial light weight aggregate, cold bonding, Fly ash, Fly ash aggregate, Pelletization, and Sintering

Clade C HIV-1 isolates circulating in Southern Africa exhibit a greater frequency of dicysteine motif-containing Tat variants than those in Southeast Asia and cause increased neurovirulence

Background: HIV-1 Clade C (Subtype C; HIV-1C) is responsible for greater than 50% of infections worldwide. Unlike clade B HIV-1 (Subtype B; HIV-1B), which is known to cause HIV associated dementia (HAD) in approximately 15% to 30% of the infected individuals, HIV-1C has been linked with lower prevalence of HAD (0 to 6%) in India and Ethiopia. However, recent studies report a higher prevalence of HAD in South Africa, Zambia and Botswana, where HIV-1C infections predominate. Therefore, we examined whether Southern African HIV-1C is genetically distinct and investigated its neurovirulence. HIV-1 Tat protein is a viral determinant of neurocognitive dysfunction. Therefore, we focused our study on the variations seen in tat gene and its contribution to HIV associated neuropathogenesis. Results: A phylogenetic analysis of tat sequences of Southern African (South Africa and Zambia) HIV isolates with those from the geographically distant Southeast Asian (India and Bangladesh) isolates revealed that Southern African tat sequences are distinct from Southeast Asian isolates. The proportion of HIV − 1C variants with an intact dicysteine motif in Tat protein (C30C31) was significantly higher in the Southern African countries compared to Southeast Asia and broadly paralleled the high incidence of HAD in these countries. Neuropathogenic potential of a Southern African HIV-1C isolate (from Zambia; HIV-1C1084i), a HIV-1C isolate (HIV-1IndieC1) from Southeast Asia and a HIV-1B isolate (HIV-1ADA) from the US were tested using in vitro assays to measure neurovirulence and a SCID mouse HIV encephalitis model to measure cognitive deficits. In vitro assays revealed that the Southern African isolate, HIV-1C1084i exhibited increased monocyte chemotaxis and greater neurotoxicity compared to Southeast Asian HIV-1C. In neurocognitive tests, SCID mice injected with MDM infected with Southern African HIV-1C1084i showed greater cognitive dysfunction similar to HIV-1B but much higher than those exposed to Southeast Asian HIV − 1C. Conclusions: We report here, for the first time, that HIV-1C from Southern African countries is genetically distinct from Southeast Asian HIV-1C and that it exhibits a high frequency of variants with dicysteine motif in a key neurotoxic HIV protein, Tat. Our results indicate that Tat dicysteine motif determines neurovirulence. If confirmed in population studies, it may be possible to predict neurocognitive outcomes of individuals infected with HIV-1C by genotyping Tat

Dioxin Exposure Blocks Lactation Through a Direct Effect on Mammary Epithelial Cells Mediated by the Aryl Hydrocarbon Receptor Repressor

In mammals, lactation is a rich source of nutrients and antibodies for newborn animals. However, millions of mothers each year experience an inability to breastfeed. Exposure to several environmental toxicants, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), has been strongly implicated in impaired mammary differentiation and lactation. TCDD and related polyhalogenated aromatic hydrocarbons are widespread industrial pollutants that activate the aryl hydrocarbon receptor (AHR). Despite many epidemiological and animal studies, the molecular mechanism through which AHR signaling blocks lactation remains unclear. We employed in vitro models of mammary differentiation to recapitulate lactogenesis in the presence of toxicants. We demonstrate AHR agonists directly block milk production in isolated mammary epithelial cells. Moreover, we define a novel role for the aryl hydrocarbon receptor repressor (AHRR) in mediating this response. Our mechanistic studies suggest AHRR is sufficient to block transcription of the milk gene β-casein. Since TCDD is a prevalent environmental pollutant that affects women worldwide, our results have important public health implications for newborn nutrition

Negative phenotypic and genetic associations between copulation duration and longevity in male seed beetles

Reproduction can be costly and is predicted to trade-off against other characters. However, while these trade-offs are well documented for females, there has been less focus on aspects of male reproduction. Furthermore, those studies that have looked at males typically only investigate phenotypic associations, with the underlying genetics often ignored. Here, we report on phenotypic and genetic trade-offs in male reproductive effort in the seed beetle, Callosobruchus maculatus. We find that the duration of a male's first copulation is negatively associated with subsequent male survival, phenotypically and genetically. Our results are consistent with life-history theory and suggest that like females, males trade-off reproductive effort against longevity