881 research outputs found
Performance Comparison of Symmetric and Offset Reflector Antennas Adaptively Illuminated by Novel Triple Mode Feedhorn
Parabolic symmetric and offset reflector antennas adaptively illuminated using a novel triple-mode feedhorn (TE11+TM01+TE21) with different mode combinations and impedance and radiation performances are presented. The combination of the radiating modes in a feedhorn with proper amplitude and fixed phase values helps in electronically pointing the main beam of the radiating patterns such as that obtained in a beam-steering antenna with limited beam-scan range. This type of radiation performance virtually creates a displaced phase center location for the feedhorn, which, consequently, adaptively illuminates the reflector antenna surface. Impedance-matching bandwidths are preserved for both reflector antennas similar to the case of feedhorn alone. The copolarization gain and peak cross-polarization levels are far better with the offset reflector antenna than the symmetric reflector antenna. Such reflector antennas find applications in ground moving target indicator (GMTI) and space based radars. The investigation results are solely computed using FEKO full-wave analysis tool
EVALUATION OF PHYTASE PRODUCTION BY HYPOCREA LIXII SURT01 IN SUBMERGED AND SOLID-STATE FERMENTATIONS
Objective: Phytases have important applications in human and animal nutrition because they hydrolyze the phytate present in legumes, cereal grains and oil seeds to release inorganic phosphate. Supplementation of phosphate to the poultry causes a serious problem of eutrophication. This can be reduced by incorporating phytase in poultry feed. Present study explains extracellular phytase production by SmF and SSF from a fungal strain Hypocrea lixii SURT01.
Methods: Extracellular phytase production by Hypocrea lixii SURT01 was evaluated in media containing various refined carbon sources (Fructose, Sucrose, Maltose and lactose in concentration ranging from 1.5% to 7.5%) along with standard medium under submerged fermentation (SmF). At the same time, phytase production was studied under Solid State Fermentation (SSF) with four different substrate such as barley, green gram, bengal gram and black gram.
Results: In SmF out of different carbon sources in various concentrations, 6% sucrose showed maximum enzyme production (245U/ml). In SSF, barley showed highest phytase yield (1638 Units/ml) on 5th day of incubation.
Conclusion: Evaluation of Solid state fermentation showed enhanced phytase production when compared to Submerged Fermentation
Intra-operative peri-articular cocktail injection in inflammatory arthritis patients undergoing total knee arthroplasty
Pain following TKA is often severe in most patients. The purpose of this case series was to assess the efficiency of intra-operative peri-articular cocktail injection in management of pain following total knee arthroplasty. This case series involves 16 patients with inflammatory arthritis of knee undergoing total knee arthroplasty (TKA). All patients had received peri-articular cocktail of drugs before the implantation of prosthesis with cement. In our study, there was significant improvement of Knee Clinical Score and Knee Functional Score following TKA. The mean KSS score was 37.5 (range: 31-44) improved to 92.5 (range, 86-99) and the functional score improved from 25.5 (range, 18-33) to 76 (range, 72- 80) at 6 months and 93 (range: 90-96) at 12 months. Intraoperative peri-articular injection with 20 ml of 0.5% ropivacaine, 1 ml of ketorolac, 1ml of clonidine and 0.5ml noradrenaline diluted in 20 ml of saline is effective in reducing immediate post-operative pain and thereby improving the overall functional outcome
Antibacterial property of neem nanoemulsion against Vibrio anguillarium infection in Asian sea bass (Lates calcarifer)
1222-1226Fish vibriosis is among the most common diseases that is caused by a bacteria belonging to the genus Vibrio (Vibrio anguillarium). It causes considerable economic loss in the commercial cultivation of Asian sea bass (Lates calcarifer). The resistance of microorganisms to antibiotics has resulted in a growing need for developing a new antibacterial therapy that is effective in aquaculture. The aim of this study is to develop neem nanoemulsion with antibacterial activity against V. anguillarium to identify a possible alternative to the commonly used antibiotics in aquaculture. Neem nanoemulsion was prepared and the effectiveness was studied both in vitro and in vivo (agar well diffusion assay and artificial infection). Injection and immersion challenge of neem nanoemulsion formulated the fish less susceptible to V. anguillarium infection. The results confirmed the potential use of neem nanoemulsion as a source of antibacterial compounds or as a health-promoting medicine for fish culture
Increased pump acceptance bandwidth in spontaneous parametric downconversion process using Bragg reflection waveguides
In this paper we show that by suitably tailoring the dispersion
characteristics of a Bragg reflection waveguide (BRW) mode, it is possible to
achieve efficient photon pair generation over a large pump bandwidth while
maintaining narrow signal bandwidth. The structure proposed consists of a high
index core BRW with a periodically poled GaN core and periodically stratified
cladding made up of alternate layers of and
. Such photon-pair generators should find applications in
realizing compact and stable sources for quantum information processing.Comment: 6 pages, 5 figure
In vivo evaluation of PhiC31 recombinase activity using a self-excision cassette
Gene targeting allows precise tailoring of the mouse genome such that desired modifications can be introduced under precise temporal and spatial control. This can be achieved through the use of site-specific recombinases, which mediate deletion or inversion of genomic DNA flanked by recombinase-specific recognition sites, coupled with gene targeting to introduce the recombinase recognition sites at the desired genomic locations within the mouse genome. The introduction of multiple modifications at the same locus often requires use of multiple recombination systems. The most commonly used recombination system is Cre/lox. We here evaluated in vivo the ability of PhiC31 phage integrase to induce a genomic deletion in mouse. We engineered a self-excision cassette, modeled after one previously designed for Cre, containing a positive selection marker and PhiC31 driven by a testis-specific promoter, all flanked by PhiC31 specific attP/B sites. We found in vivo PhiC31 mediated self-excision in 38% of transmitted alleles, although 18% of these showed evidence of imprecise deletion. Furthermore, in the 69% of un-recombined cassettes, sequence analysis revealed that PhiC31 mediated an intra-molecular deletion of the attB site preventing any subsequent recombination. This study demonstrates that PhiC31 can be used to automatically remove Neo, in the male chimera germline, although it is not as efficient or as accurate as Cre
The nucleus of the living zygote of Saccharomyces carlsbergensis
1. The zygotes ofSaccharomyces carlsbergensis retain the shape assumed at the time of their origin by the fusion of two spores even after they have produced a few buds. 2. The nucleus is visible in the zygotes on the 7th day after the introduction of spores into fresh wort. A nuclear membrane delimits the nucleus from the cytoplasm and encloses formed structures. The structure of the nucleus is identical in a zygote and its bud. 3. Iodine-formaldehyde-acetic gives a life-like preservation of cell structures. After removal of the cytoplasmic basophilia by acid hydrolysis, staining with hæmatoxylin gives pictures of nuclear details comparable to that of the living nucleus. The Feulgen stained area is only a portion of the resting nucleus
Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans
Few mating-regulated genes have been characterized in Candida albicans. C. albicans FIG1 (CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1 was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca(2+) uptake in mating-competent opaque cells. Uptake was reduced substantially in the fig1Δ null mutant. CaFig1 is therefore involved in Ca(2+) influx and localizes to membranes that are destined to undergo fusion during mating
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