Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.

Neural cell adhesion molecule (N-CAM) is a membrane glycoprotein expressed on neural and muscle tissues that is involved in homotypic adhesive interactions. We have demonstrated that N-CAM also is expressed on hematopoietic cells, and is recognized by the anti-Leu-19 mAb. Leu-19 is preferentially expressed on NK cells and T lymphocytes that mediate MHC-unrestricted cytotoxicity, but is also present on some myeloid leukemia cell lines. On NK cells, T cells, the KG1a.5 hematopoietic cell line, and a neuroblastoma cell line, Leu-19 is a approximately 140-kD polypeptide with N-linked carbohydrates and abundant sialic acid residues. Sequential immunoprecipitation and peptide mapping demonstrated that the Leu-19 and N-CAM molecules expressed on leukocyte and neuroblastoma cell lines are similar structures. These findings suggest that the Leu-19 antigen on leukocytes may be involved in cell adhesion, analogous to the function on N-CAM on neural cells

Protostellar clusters in intermediate-mass (IM) star forming regions

The transition between the low density groups of T Tauri stars and the high density clusters around massive stars occurs in the intermediate-mass (IM) range (M$_*$$\sim$2--8 M$_\odot$). High spatial resolution studies of IM young stellar objects (YSO) can provide important clues to understand the clustering in massive star forming regions. Aims: Our aim is to search for clustering in IM Class 0 protostars. The high spatial resolution and sensitivity provided by the new A configuration of the Plateau de Bure Interferometer (PdBI) allow us to study the clustering in these nearby objects. Methods: We have imaged three IM Class 0 protostars (Serpens-FIRS 1, IC 1396 N, CB 3) in the continuum at 3.3 and 1.3mm using the PdBI. The sources have been selected with different luminosity to investigate the dependence of the clustering process on the luminosity of the source. Results: Only one millimeter (mm) source is detected towards the low luminosity source Serpens--FIRS 1. Towards CB 3 and IC1396 N, we detect two compact sources separated by $\sim$0.05 pc. The 1.3mm image of IC 1396 N, which provides the highest spatial resolution, reveal that one of these cores is splitted in, at least, three individual sources.Comment: 4 pages, 3 figures, accepted for publication in Astronomy and Astrophysics Letters (Special Feature IRAM/PdB

A UV-to-MIR monitoring of DR Tau: exploring how water vapor in the planet formation region of the disk is affected by stellar accretion variability

Young stars are known to show variability due to non-steady mass accretion rate from their circumstellar disks. Accretion flares can produce strong energetic irradiation and heating that may affect the disk in the planet formation region, close to the central star. During an extreme accretion outburst in the young star EX Lupi, the prototype of EXor variables, remarkable changes in molecular gas emission from $\sim1$ AU in the disk have recently been observed (Banzatti et al. 2012). Here, we focus on water vapor and explore how it is affected by variable accretion luminosity in T Tauri stars. We monitored a young highly variable solar-mass star, DR Tau, using simultaneously two high/medium-resolution ESO-VLT spectrographs: VISIR at 12.4 $\mu$m to observe water lines from the disk, and X-shooter covering from 0.3 to 2.5 $\mu$m to constrain the stellar accretion. Three epochs spanning timescales from several days to several weeks were obtained. Accretion luminosity was estimated to change within a factor $\sim2$, and no change in water emission was detected at a significant level. In comparison to EX Lupi and EXor outbursts, DR Tau suggests that the less long-lived and weaker variability phenomena typical of T Tauri stars may leave water at planet-forming radii in the disk mostly unaffected. We propose that these systems may provide evidence for two processes that act over different timescales: UV photochemistry in the disk atmosphere (faster) and heating of the disk deeper layers (slower).Comment: 8 pages, 7 figures, accepted for publication in The Astrophysical Journa

Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase.

Intracellular pathways leading from membrane receptor engagement to apoptotic cell death are still poorly characterized. We investigated the intracellular signaling generated after cross-linking of CD95 (Fas/Apo-1 antigen), a broadly expressed cell surface receptor whose engagement results in triggering of cellular apoptotic programs. DX2, a new functional anti-CD95 monoclonal antibody was produced by immunizing mice with human CD95-transfected L cells. Crosslinking of CD95 with DX2 resulted in the activation of a sphingomyelinase (SMase) in promyelocytic U937 cells, as well as in other human tumor cell lines and in CD95-transfected murine cells, as demonstrated by induction of in vivo sphingomyelin (SM) hydrolysis and generation of ceramide. Direct in vitro measurement of enzymatic activity within CD95-stimulated U937 cell extracts, using labeled SM vesicles as substrates, showed strong SMase activity, which required pH 5.0 for optimal substrate hydrolysis. Finally, all CD95-sensitive cell lines tested could be induced to undergo apoptosis after exposure to cell-permeant C2-ceramide. These data indicate that CD95 cross-linking induces SM breakdown and ceramide production through an acidic SMase, thus providing the first information regarding early signal generation from CD95, and may be relevant in defining the biochemical nature of intracellular messengers leading to apoptotic cell death

First results from a VLBA proper motion survey of H2O masers in low-mass YSOs: the Serpens core and RNO15-FIR

This article reports first results of a long-term observational program aimed to study the earliest evolution of jet/disk systems in low-mass YSOs by means of VLBI observations of the 22.2 GHz water masers. We report here data for the cluster of low-mass YSOs in the Serpens molecular core and for the single object RNO~15-FIR. Towards Serpens SMM1, the most luminous sub-mm source of the Serpens cluster, the water maser emission comes from two small (< 5 AU in size) clusters of features separated by ~25 AU, having line of sight velocities strongly red-shifted (by more than 10 km/s) with respect to the LSR velocity of the molecular cloud. The two maser clusters are oriented on the sky along a direction that is approximately perpendicular to the axis of the radio continuum jet observed with the VLA towards SMM1. The spatial and velocity distribution of the maser features lead us to favor the interpretation that the maser emission is excited by interaction of the receding lobe of the jet with dense gas in the accretion disk surrounding the YSO in SMM1. Towards RNO~15-FIR, the few detected maser features have both positions and (absolute) velocities aligned along a direction that is parallel to the axis of the molecular outflow observed on much larger angular scales. In this case the maser emission likely emerges from dense, shocked molecular clumps displaced along the axis of the jet emerging from the YSO. The protostar in Serpens SMM1 is more massive than the one in RNO~15-FIR. We discuss the case where a high mass ejection rate can generate jets sufficiently powerful to sweep away from their course the densest portions of circumstellar gas. In this case, the excitation conditions for water masers might preferably occur at the interface between the jet and the accretion disk, rather than along the jet axis.Comment: 18 pages (postscript format); 9 figures; to be published into Astronomy & Astrophysics, Main Journa

Hot and dense water in the inner 25 AU of SVS13-A

In the context of the ASAI (Astrochemical Surveys At IRAM) project, we carried out an unbiased spectral survey in the millimeter window towards the well known low-mass Class I source SVS13-A. The high sensitivity reached (3-12 mK) allowed us to detect at least 6 HDO broad (FWHM ~ 4-5 km/s) emission lines with upper level energies up to Eu = 837 K. A non-LTE LVG analysis implies the presence of very hot (150-260 K) and dense (> 3 10^7 cm-3) gas inside a small radius ($\sim$ 25 AU) around the star, supporting, for the first time, the occurrence of a hot corino around a Class I protostar. The temperature is higher than expected for water molecules are sublimated from the icy dust mantles (~ 100 K). Although we cannot exclude we are observig the effects of shocks and/or winds at such small scales, this could imply that the observed HDO emission is tracing the water abundance jump expected at temperatures ~ 220-250 K, when the activation barrier of the gas phase reactions leading to the formation of water can be overcome. We derive X(HDO) ~ 3 10-6, and a H2O deuteration > 1.5 10-2, suggesting that water deuteration does not decrease as the protostar evolves from the Class 0 to the Class I stage.Comment: MNRAS Letter

A Keplerian gaseous disk around the B0 star R Mon

We present high-angular resolution observations of the circumstellar disk around the massive Herbig Be star R Mon (M~8 Msun) in the continuum at 2.7mm and 1.3mm and the CO 1->0 and 2->1 rotational lines. Based on the new 1.3mm continuum image we estimate a disk mass (gas+dust) of 0.007 Msun and an outer radius of <150 AU. Our CO images are consistent with the existence of a Keplerian rotating gaseous disk around this star. Up to our knowledge, this is the most clear evidence for the existence of Keplerian disks around massive stars reported thus far. The mass and physical characteristics of this disk are similar to thoseof the more evolved T Tauri stars and indicate a shorter timescale for the evolution and dispersal of circumstellar disks around massive stars which lose most of their mass before the star becomes visible.Comment: 5 page

The kinematics of molecular clumps surrounding hot cores in G29.96-0.02 and G31.41+0.31

We present high angular resolution interferometric observations of the 3 and 1.3mm continuum emission, and HCO+(1-0) and SiO(2-1)v=0 lines, obtained with the Owens Valley Radio Observatory millimeter-wave array, toward two hot cores (HCs) associated with two well known ultracompact (UC) HII regions: G29.96-0.02 and G31.41+0.31. These HCs are believed to host young forming massive stars which have been suggested to be surrounded by massive rotating accretion disks. The aim of these new observations is to study the structure and kinematics of the molecular clumps surrounding the HCs and nearby UCHII regions at moderately high angular resolution. Our observations reveal that the clumps within which the HCs and UCHII regions are embedded have a complex kinematical structure. The total mass of the clumps is estimated to be in the range 1000-3000 Msun, consistent with previous findings. Our observations also show compelling evidence that the clump in G29.96-0.02 is contracting onto the HC position, suggesting that the accretion process onto the massive young stellar object embedded in the HC is still ongoing. In these objects the kinematical structure that we observe is also compatible with the presence of a massive rotating disk within the HC, even though we cannot prove this suggestion with our data. The case of G31.41+0.31 is more complicated, and our data, although consistent with the presence of an inner disk and an infalling envelope around it, do not have the required spatial resolution to resolve the different structures.Comment: 13 pages, 15 figs, A&A in pres