Effect of plant-growth-promoting rhizobacteria inoculation on plant growth, productivity and economics of Basmati rice

A field experiment was conducted in a wet season (Kharif) to study the effects of plant growth-promoting rhizobacteria(PGPR) inoculation on agronomic traits and productivity of Basmati rice (cv. ‘Pusa Basmati 1401’) in a randomized block with twelve treatments. We evaluated one bacterial (Providencia sp. PW5) and one cyanobacterial strain (Anabaena sp. CR3), and also a Multani mitti (Fuller’s earth)-based blue-green algal biofertilizer (a composite culture of Anabaena, Nostoc, Tolypothrix and Aulosira sp.). Plant growth, yield attributes, yield and net return of rice were significantly improved due to the rhizobacterial inoculation. The highest responses were recorded from combined inocula of bacteria and cyanobacteria together with compost.Keywords: Bacterial inoculants; BGA; cyanobacterial inoculants, PGPR; yield attributes

Influence of organic nutrient sources and moisture management on productivity, biofortification and soil health in pearl millet (Pennisetum glaucum) + clusterbean (Cyamopsis tetragonaloba) intercropping system of semi-arid India

A field experimentation was carried out at New Delhi, India for three years (2010-12) under rainfed semi-arid conditions to assess the effect of three organic nutrient sources and two moisture management practices on productivity, biofortification and soil health under pearl millet [Pennisetum glaucum (L.) R. Br. emend Stuntz] + clusterbean [Cyamopsis tetragonaloba (L.) Taub] intercropping system (PCIS). In current study, the pearl millet grain yield under sole pearl millet was at par with PCIS while its stover yield reduced by 5%. Under PCIS, inclusion of clusterbean led to significant reduction in clusterbean yield compared to its sole stand, however, PCIS system productivity was more over their sole crop stands, indicating that additive series of PCIS may bring more net economic gains over their sole stands in semi-arid ecology of India. The coarse cereal-legume interactions under PCIS have also led to improved protein content in pearl millet (11.2%) over its sole stand (10.7%). Zinc (Zn) and iron (Fe) deficiency is a common feature in semi-arid India and the crops grown as well. Study revealed that Zn and Fe content in pearl millet were increased by 6.5 and 1.6%, respectively, by the inclusion of clusterbean in PCIS. Plant growth, yield attributes and yield of pearl millet and clusterbean crops showed an improvement under flat sowing + organic mulching (FS+OM) over ridge and furrow sowing (RFS) but the differences were non-significant. Among organic nutrient sources, leaf compost (LC) @ 10 t/ha and FYM @ 10 t/ha produced significantly more system productivity in terms of pearl millet-equivalent-yield (PEY) than 100% RDF and control. Protein content both in pearl millet and clusterbean, and Zn and Fe biofortification in pearl millet grains was higher under FYM treated plots closely followed by LC, and leaf + cowdung mixture compost. Application of organics especially FYM @ 10 t/ha also resulted in improved soil health in terms of bulk density, soil organic carbon and NPK status after three years experimentation

Analysis of farmers' communication network and factors of knowledge regarding agro meteorological parameters

Understanding communication networks bear significant importance for undertaking interventions regarding local information needs. It becomes more important if the issues of sensitivity like climate change and its effect on agriculture has to be dealt. The agricultural sector needs accurate, reliable and timely weather and climate information for daily tactical decisions and long-term planning for crop management. They can potentially alter selection and management of crops based on new information obtained within a few weeks or days of planting. The information systems integrating agricultural educators, researchers, extensionists and farmers need to be introduced for agriculture sector for which sufficient data base regarding the gap between the possessed knowledge and the available knowledge and the information source utilization pattern need to be generated. The present study to find out the factors of the knowledge of farmers regarding climate and weather in relation to crop management and the analysis of farmers' communication network was undertaken in Adilabad district (highly vulnerable to climate change effects) of Telangana state of India. A knowledge test specifically designed was administered to 120 respondents and was found that majority of the respondents had moderate level of knowledge. The socio-economic and psycho-personal variables like education, occupation, family type, land holding, social participation, economic motivation, innovativeness, scientific orientation and risk orientation showed positive and significant relationship with knowledge level and in regression analysis education, scientific orientation were positive and had significant contribution to the knowledge level of respondents. Television, radio, agriculture officer and progressive farmers were categorized into strong group of information sources usefulness. In order to support farm level decisions and minimize the loses in adverse climatic and weather conditions farmers' understanding about interaction of climate and agro-ecosystem need to be bridged through inclusion of farmers' communication networ

Moisture management and zinc fortification impacts on economics, quality and nutrient uptake of pearlmillet (Pennisetum glaucum) under rainfed conditions

A field study was conducted during kharif seasons of 2012 and 2013 at the research farm of ICAR-Indian Agricultural Research Institute, New Delhi to find out the effect of moisture management and zinc fortification on economics, quality and nutrient uptake of pearlmillet (Pennisetum glaucum L.) in pearlmillet-chickpea cropping system under rainfed conditions. Sowing of pearlmillet on flat bed with 5.0 tonnes/ha crop residue fetched significantly higher net returns of ` 25408/ha with production efficiency of ` 279.2/ha/day and protein content in grain during first year and B:C ratio during both the years as compared to flat bed without crop residue. However, in terms of net returns (` 30578/ha), production efficiency (` 397.1/ha/day) and protein content during second year and zinc content in both grain and stover and total uptake of N, P, K, Zn, Fe, Mn and Cu during both the years of study, flat bed with 5.0 tonnes/ha crop residue proved to be significantly superior over flat bed without crop residue as well as flat bed with 2.5 tonnes/ha crop residue and performed in statistically similar way to narrow bed and furrow with 2.5 tonnes/ha crop residue. Under zinc fortification treatments direct application of 2.5 and 5.0 kg Zn/ha being at par with each other and recorded significantly higher net returns, B:C ratio, production efficiency, protein content and total uptake of P, K and Cu during both the years and total uptake of Fe and Mn during second year over control. Content of Zn in both grain and stover and total uptake of N and Zn during both the years of study were improved significantly with increasing levels of zinc fortification up to 5.0 kg Zn/ha. Residual effect of zinc fortification was observed during second and showed significant response up to 5.0 kg Zn/ha on net returns, B:C ratio, production efficiency, Zn content in both grain and stover and total uptake of N, K and Zn and up to 2.5 kg Zn/ha on protein content and total uptake of P, Fe, Mn and Cu by pearlmillet

Influence of plant growth-promoting rhizobacteria inoculation on nutrient availability, soil microbial properties and defence enzymes in rice (Oryza sativa) crop

Soil organic carbon, available N, P, K, Zn, Fe and Cu in soil at crop harvest stage significantly increased due to the integrated inoculation of PGPR and Multani mitti based BGA with application of compost and chemical N fertilizer (80 and 120 kg/ha) over N control. Status of SOC and available N, P, K, Fe, Zn and Cu in soil at crop maturity stage was highest at 2/3N+BI+CI+C@ 5.0 tonnes/ha. Soil chlorophyll, dehydrogenase and ARA activity were significantly higher at 2/3N+BI+CI+C@ 5.0 tonnes/ha compared to other treatments. Plant enzymatic activity of POD and PPO at 60 DAT also was significantly higher in 2/3N+BI+CI+Compost@ 5.0 tonnes/ha. Application of 2/ 3N with inoculation of bacterial and cyanobacterial PGPR along with 5.0 tonnes/ha compost not only improved nutrient availability in soil but also enhanced soil microbial, plant enzymatic activity and crop yield

Formation of ER-lumenal intermediates during export of Plasmodium proteins containing transmembrane-like hydrophobic sequences

During the blood stage of a malaria infection, malaria parasites export both soluble and membrane proteins into the erythrocytes in which they reside. Exported proteins are trafficked via the parasite endoplasmic reticulum and secretory pathway, before being exported across the parasitophorous vacuole membrane into the erythrocyte. Transport across the parasitophorous vacuole membrane requires protein unfolding, and in the case of membrane proteins, extraction from the parasite plasma membrane. We show that trafficking of the exported Plasmodium protein, Pf332, differs from that of canonical eukaryotic soluble-secreted and transmembrane proteins. Pf332 is initially ER-targeted by an internal hydrophobic sequence that unlike a signal peptide, is not proteolytically removed, and unlike a transmembrane segment, does not span the ER membrane. Rather, both termini of the hydrophobic sequence enter the ER-lumen and the ER-lumenal species is a productive intermediate for protein export. Furthermore, we show in intact cells, that two other exported membrane proteins, SBP1 and MAHRP2, assume a lumenal topology within the parasite secretory pathway. Although the addition of a C-terminal ER-retention sequence, recognised by the lumenal domain of the KDEL receptor, does not completely block export of SBP1 and MAHRP2, it does enhance their retention in the parasite ER. This indicates that a sub-population of each protein adopts an ER-lumenal state that is an intermediate in the export process. Overall, this suggests that although many exported proteins traverse the parasite secretory pathway as typical soluble or membrane proteins, some exported proteins that are ER-targeted by a transmembrane segment-like, internal, non-cleaved hydrophobic segment, do not integrate into the ER membrane, and form an ER-lumenal species that is a productive export intermediate. This represents a novel means, not seen in typical membrane proteins found in model systems, by which exported transmembrane-like proteins can be targeted and trafficked within the lumen of the secretory pathway

Empowering Students’ Learning Achievement Through Project-Based Learning As Perceived By Electrical Instructors And Students

Purposes of this research were to find out factors empowering electrical students‘ learning achievement through Project-Based Learning (PBL) as perceived by instructors‘ and students‘ opinions. The sample chosen for this study were 247 electrical power instructors at vocational education institutes and 161 electrical students in the 3 rd and 4th year who were studying in the 1st semester of academic year 2006 at Electrical Education Department, Faculty of Industrial Education and Technology, King Mongkut‘s University of Technology Thonburi by using simple random sampling. The instrument used for data collection was 7 rating scales questionnaire. The reliability of the instrument calculated by Cronbach Alpha Coefficient was 0.8185 and 0.9839, respectively. The data were analysed by using mean ( ), Standard Deviation (S.D.) and Analysis of Factors by Principal Component Analysis technique: PCA, orthogonal rotation axis by Varimax Method. The results of the study on factors empowering electrical students‘ learning achievement through Project-Based Learning (PBL) were as follows: both instructors and students agreed on Interesting/Attention(0.799 and 0.885, respectively) while other factors such as Planning(0.722), Sharing Ideas(0.582), Thinking(0.576), Facilitating (0.547), Constructionism (0.540), Scientific Process (0.525), Multiple Intelligence (0.479), and Goal Setting(0.453) were perceived by instructors, and students‘ opinions were on Advising/Guiding(0.863), Thinking(0.661), Goal Setting (0.634), Multiple Intelligence(0.553), Scientific Process(0.528), Assisting(0.524), and Sharing Ideas (0.492), if not more so

Factors Influencing Performance of Cholangioscopy-Guided Lithotripsy Including Available Different Technologies: A Prospective Multicenter Study with 94 Patients

Background: Peroral cholangioscopy (POC)-guided lithotripsy is an effective treatment for difficult biliary stones. A clear definition of factors associated with the efficacy of POC-guided lithotripsy in one session and the performance of electrohydraulic lithotripsy (EHL) and laser lithotripsy (LL) have not clearly emerged. Methods: This was a non-randomized prospective multicenter study of all consecutive patients who underwent POC lithotripsy (using EHL and/or LL) for difficult biliary stones. The primary endpoint of the study was the number of sessions needed to achieve complete ductal clearance and the factors associated with this outcome. Secondary endpoints included the evaluated efficacies of LL and EHL. Results: Ninety-four patients underwent 113 procedures of EHL or LL. Complete ductal clearance was obtained in 93/94 patients (98.94%). In total, 80/94 patients (85.11%) achieved stone clearance in a single session. In the multivariate analysis, stone size was independently associated with the need for multiple sessions to achieve complete ductal clearance (odds ratio = 1.146, 95% confidence interval: 1.055-1.244; p = 0.001). Using ROC curves and the Youden index, 22 mm was found to be the optimal cutoff for stone size (95% confidence interval: 15.71-28.28; p < 0.001). The majority of the patients (62.8%) underwent LL in the first session. Six patients failed the first session with EHL after using two probes and therefore were crossed over to LL, obtaining ductal clearance in a single additional session with a single LL fiber. EHL was significantly associated with a larger number of probes (2.0 vs. 1.02) to achieve ductal clearance (p < 0.01). The mean procedural time was significantly longer for EHL than for LL [72.1 (SD 16.3 min) versus 51.1 (SD 10.5 min)] (p < 0.01). Conclusions: POC is highly effective for difficult biliary stones. Most patients achieved complete ductal clearance in one session, which was significantly more likely for stones < 22 mm. EHL was significantly associated with the need for more probes and a longer procedural time to achieve ductal clearance.info:eu-repo/semantics/publishedVersio

Valuing health states: is the MACBETH approach useful for valuing EQ-5D-3L health states?

Background Quality Adjusted Life Years (QALYs) are a key outcome measure widely used within health technology assessment and health service research studies. QALYs combine quantity and quality of life, with quality of life calculations relying on the value of distinct health states. Such health states’ values capture the preferences of a population and have been typically built through numerical elicitation methods. Evidence points to these value scores being influenced by methods in use and individuals reporting cognitive difficulties in eliciting their preferences. Evidence from other areas has further suggested that individuals may prefer using distinct elicitation techniques and that this preference can be influenced by their numeracy. In this study we explore the use of the MACBETH (Measuring Attractiveness by a Categorical Based Evaluation Technique) non-numerical preference elicitation approach for health states’ evaluation. Methods A new protocol for preference elicitation based on MACBETH (only requiring qualitative judgments) was developed and tested within a web survey format. A sample of the Portuguese general population (n=243) valued 25 EQ-5D-3L health states with the MACBETH protocol and with a variant of the time trade-off (TTO) protocol, for comparison purposes and for understanding respondents’ preference for distinct protocols and differences in inconsistent evaluations. Respondents answered to a short numeracy test, and basic socio-economic information collected. Results Results show that the mean values derived from MACBETH and the TTO variant are strongly correlated; however, there are substantial differences for several health states’ values. Large and similar numbers of logical inconsistencies were found in respondents’ answers with both methods. Participants with higher levels of numeracy according to the test preferred expressing value judgments with MACBETH, while participants with lower levels were mostly indifferent to both methods. Higher correlations between MACBETH and TTO variant evaluations were observed for individuals with higher numeracy. Conclusion Results suggest that it is worth researching the use of non-numerical preference elicitation methods. Numeracy tests more appropriate for preference elicitation when no explicit considerations of uncertainty are made need to be explored and used. Further behavioural research is needed to fully understand the potential for using these methods in distinct settings (e.g. in different evaluation contexts and in face-to-face and non-face-to-face environments), as well as to explore the effect of literacy on assessments and on respondents’ preferences.UID/MULTI/4066/2016info:eu-repo/semantics/publishedVersio

Microbes with higher metabolic independence are enriched in human gut microbiomes under stress

A wide variety of human diseases are associated with loss of microbial diversity in the human gut, inspiring a great interest in the diagnostic or therapeutic potential of the microbiota. However, the ecological forces that drive diversity reduction in disease states remain unclear, rendering it difficult to ascertain the role of the microbiota in disease emergence or severity. One hypothesis to explain this phenomenon is that microbial diversity is diminished as disease states select for microbial populations that are more fit to survive environmental stress caused by inflammation or other host factors. Here, we tested this hypothesis on a large scale, by developing a software framework to quantify the enrichment of microbial metabolisms in complex metagenomes as a function of microbial diversity. We applied this framework to over 400 gut metagenomes from individuals who are healthy or diagnosed with inflammatory bowel disease (IBD). We found that high metabolic independence (HMI) is a distinguishing characteristic of microbial communities associated with individuals diagnosed with IBD. A classifier we trained using the normalized copy numbers of 33 HMI-associated metabolic modules not only distinguished states of health versus IBD, but also tracked the recovery of the gut microbiome following antibiotic treatment, suggesting that HMI is a hallmark of microbial communities in stressed gut environments