A proposito dell\u2019inchiesta della S. Congregazione dell\u2019Indice dei libri proibiti di fine \u2018500

Il paper \u2013 diviso in tre parti \u2013 muove dai risultati della \u201cRicerca sull\u2019inchiesta della Congregazione dell\u2019Indice\u201d che ha preso in esame i codici Vaticani Latini che conservano circa 9500 liste di titoli di libri posseduti dai religiosi italiani alla fine del XVI secolo. Roberto Rusconi sviluppa una panoramica introduttiva sulle biblioteche degli ordini religiosi alla fi ne del Cinquecento. Giovanna Granata sfrutta le molteplici potenzialit\ue0 informative dell\u2019Inchiesta, sia in termini di conoscenza di tali biblioteche, sia rispetto alle modalit\ue0 di circolazione delle diverse edizioni. Rosa Marisa Borraccini prende invece in esame un caso di studio come quello del 'Confessionario' di Girolamo da Palermo per dimostrare l\u2019importanza che un testo oggi quasi del tutto sconosciuto aveva all\u2019epoca della sua produzione. This paper \u2013 divided into three sections \u2013 expands upon the work undertaken in \u201cRicerca sull\u2019inchiesta della Congregazione dell\u2019Indice\u201d, which has examined the Codici Vaticani Latini, a collection which conserves around 9.500 lists of volumes owned by Italian monks and clerics at the end of the sixteenth century. Roberto Rusconi offers an introductory overview of the libraries of religious orders at the end of the sixteenth century. Giovanna Granata analyses the findings of the Inchiesta in terms of actual library holdings and in terms of the circulation of different editions. Rosa Marisa Borraccini examines the case study of the \u201cConfessionario\u201d of Girolamo da Palermo to demonstrate the importance certain texts, although now forgotten and largely ignored, had at the time of their production and circulation

Upgrading Monocytes Therapy for Critical Limb Ischemia Patient Treatment: Pre-Clinical and GMP-Validation Aspects

Advanced cell therapy medicinal products (ATMP) are at the forefront of a new range of biopharmaceuticals. The use of ATMP has evolved and increased in the last decades, representing a new approach to treating diseases that are not effectively managed with conventional treatments. The standard worldwide recognized for drug production is the Good Manufacturing Practices (GMP), widely used in the pharma production of synthesized drugs but applying also to ATMP. GMP guidelines are worldwide recognized standards to manufacture medicinal products to guarantee high quality, safety, and efficacy. In this report, we describe the pre-clinical and the GMP upgrade of peripheral blood mononuclear cell (PBMC) preparation, starting from peripheral blood and ending up with a GMP-grade clinical product ready to be used in patients with critical limb ischemia (CLI). We also evaluated production in hypoxic conditions to increase PBMC functional activity and angiogenic potential. Furthermore, we extensively analyzed the storage and transport conditions of the final product as required by the regulatory body for ATMPs. Altogether, results suggest that the whole manufacturing process can be performed for clinical application. Peripheral blood collected by a physician should be transported at room temperature, and PBMCs should be isolated in a clean room within 8 h of venipuncture. Frozen cells can be stored in nitrogen vapors and thawed for up to 12 months. PBMCs resuspended in 5% human albumin solution should be stored and transported at 4 degrees C before injection in patients within 24 h to thawing. Hypoxic conditioning of PBMCs should be implemented for clinical application, as it showed a significant enhancement of PBMC functional activity, in particular with increased adhesion, migration, and oxidative stress resistance. We demonstrated the feasibility and the quality of a GMP-enriched suspension of monocytes as an ATMP, tested in a clean room facility for all aspects related to production in respect of all the GMP criteria that allow its use as an ATMP. We think that these results could ease the way to the clinical application of ATMPs

Molecular identification of Contracaecum rudolphii A and B (Nematoda: Anisakidae) from cormorants collected in a freshwater ecosystem of the pre-alpine area in Northern Italy

Contracaecum rudolphii (s.l.) is a complex of sibling species with different genetic structure and ecological preference. This study reports the presence of specimens of Contracaecum rudolphii (s.l.) from sedentary and wintering cormorants (Phalacrocorax carbo sinensis) from the pre-mountain area of the Alps in Northern Italy, an important crossroads for most of the bird migration routes. A total of 48 specimens of cormorants collected from two adjacent freshwater habitats were analysed and C. rudolphii nematodes were retrieved in 100% of the examined specimens. A subsamples of 115 C. rudolphii individuals were genetically characterized and found to belong to the sibling species C. rudolphii B (n = 90) and C. rudolphii A (n = 25). C. rudolphii B were retrieved from both locations and included adults as well as larvae, while only adults of C. rudolphii A were detected, and in just one location. As expected for a freshwater environment, C. rudolphii B constitutes the largest sibling fraction, indicating that this likely is the endemic species, while cormorants originating from the breeding brackish lagoons and marine coastal environments of central and northern Europe could have brought C. rudolphii A from their breeding sites or migration stopovers

Foot Bone in Vivo: Its Center of Mass and Centroid of Shape

This paper studies foot bone geometrical shape and its mass distribution and establishes an assessment method of bone strength. Using spiral CT scanning, with an accuracy of sub-millimeter, we analyze the data of 384 pieces of foot bones in vivo and investigate the relationship between the bone's external shape and internal structure. This analysis is explored on the bases of the bone's center of mass and its centroid of shape. We observe the phenomenon of superposition of center of mass and centroid of shape fairly precisely, indicating a possible appearance of biomechanical organism. We investigate two aspects of the geometrical shape, (i) distance between compact bone's centroid of shape and that of the bone and (ii) the mean radius of the same density bone issue relative to the bone's centroid of shape. These quantities are used to interpret the influence of different physical exercises imposed on bone strength, thereby contributing to an alternate assessment technique to bone strength.Comment: 9 pages, 4 figure

Asthma Symptoms, Lung Function, and Markers of Oxidative Stress and Inflammation in Children Exposed to Oil Refinery Pollution

none12mixedF. Rusconi; CATELAN, DOLORES; G. Accetta; M. Peluso; R. Pistelli; F. Barbone; E. Di Felice; A. Munnia; P. Murgia; L. Paladini; A. Serci; BIGGERI, ANNIBALEF., Rusconi; Catelan, Dolores; G., Accetta; M., Peluso; R., Pistelli; F., Barbone; E., Di Felice; A., Munnia; P., Murgia; L., Paladini; A., Serci; Biggeri, Annibal