Adjustable viscoelasticity allows for efficient collective cell migration

Cell migration is essential for a wide range of biological processes such as embryo morphogenesis, wound healing, regeneration, and also in pathological conditions, such as cancer. In such contexts, cells are required to migrate as individual entities or as highly coordinated collectives, both of which requiring cells to respond to molecular and mechanical cues from their environment. However, whilst the function of chemical cues in cell migration is comparatively well understood, the role of tissue mechanics on cell migration is just starting to be studied. Recent studies suggest that the dynamic tuning of the viscoelasticity within a migratory cluster of cells, and the adequate elastic properties of its surrounding tissues, are essential to allow efficient collective cell migration in vivo. In this review we focus on the role of viscoelasticity in the control of collective cell migration in various cellular systems, mentioning briefly some aspects of single cell migration. We aim to provide details on how viscoelasticity of collectively migrating groups of cells and their surroundings is adjusted to ensure correct morphogenesis, wound healing, and metastasis. Finally, we attempt to show that environmental viscoelasticity triggers molecular changes within migrating clusters and that these new molecular setups modify clusters' viscoelasticity, ultimately allowing them to migrate across the challenging geometries of their microenvironment

Michael Abercrombie: contact inhibition of locomotion and more

Michael Abercrombie is regarded as one of the principal pioneers of cell biology. Although Abercrombie began his career as an experimental embryologist, working on the avian organizer with C. H. Waddington, questions on how cells in culture migrate and interact dominated his career. Whilst studying the social behaviour of chick heart embryonic fibroblasts, Abercrombie identified a phenomenon whereby colliding cells collapse their protrusions towards the cell-cell contact upon a collision, preventing their continued migration. The cells then form protrusions away from the contact and, space permitting, migrate away from each other. This behaviour is now referred to as ‘contact inhibition of locomotion’ and has been identified within embryology as the driving force behind the directional migration of the neural crest and the dispersion patterning of haemocytes and Cajal-Retzius neurons. Furthermore, its loss between collisions of cancer cells and healthy cells is associated with metastasis. In this review we begin with an overview of Abercrombie’s life and highlight some of his key publications. We then discuss Abercrombie’s discovery of contact inhibition of locomotion, the roles which cell-cell adhesions, cell-matrix adhesions and the cytoskeleton play in facilitating this phenomenon, and the importance of contact inhibition of locomotion within the living organism

Circumstellar Disk Evolution: Constraining Theories of Planet Formation

Observations of circumstellar disks around stars as a function of stellar properties such as mass, metallicity, multiplicity, and age, provide constraints on theories concerning the formation and evolution of planetary systems. Utilizing ground- and space-based data from the far-UV to the millimeter, astronomners can assess the amount, composition, and location of circumstellar gas and dust as a function of time. We review primarily results from the Spitzer Space Telescope, with reference to other ground- and space-based observations. Comparing these results with those from exoplanet search techniques, theoretical models, as well as the inferred history of our solar system, helps us to assess whether planetary systems like our own, and the potential for life that they represent, are common or rare in the Milky Way galaxy.Comment: To appear in IAU Symposium No. 258, Eds. E. Mamajek, D.R. Soderblom, and R.F.G. Wys

Model Bond albedos of extrasolar giant planets

The atmospheres of extrasolar giant planets are modeled with various effective temperatures and gravities, with and without clouds. Bond albedos are computed by calculating the ratio of the flux reflected by a planet (integrated over wavelength) to the total stellar flux incident on the planet. This quantity is useful for estimating the effective temperature and evolution of a planet. We find it is sensitive to the stellar type of the primary. For a 5 M_Jup planet the Bond albedo varies from 0.4 to 0.3 to 0.06 as the primary star varies from A5V to G2V to M2V in spectral type. It is relatively insensitive to the effective temperature and gravity for cloud--free planets. Water clouds increase the reflectivity of the planet in the red, which increases the Bond albedo. The Bond albedo increases by an order of magnitude for a 13 M_Jup planet with an M2V primary when water clouds are present. Silicate clouds, on the other hand, can either increase or decrease the Bond albedo, depending on whether there are many small grains (the former) or few large grains (the latter).Comment: 6 pages, 9 figures, uses egs.cls and epsfig.sty, submitted to Physics and Chemistry of the Earth (proceedings of the April 1998 EGS meeting in Nice, France

Exoplanet HD 209458b : Evaporation strengthened

Following re-analysis of Hubble Space Telescope observations of primary transits of the extrasolar planet HD209458b at Lyman-alpha, Ben-Jaffel (2007, BJ007) claims that no sign of evaporation is observed. Here we show that, in fact, this new analysis is consistent with the one of Vidal-Madjar et al. (2003, VM003) and supports the detection of evaporation. The apparent disagreement is mainly due to the disparate wavelength ranges that are used to derive the transit absorption depth. VM003 derives a (15+/-4)% absorption depth during transit over the core of the stellar Lyman-alpha line (from -130 km/s to +100 km/s), and this result agrees with the (8.9+/-2.1)% absorption depth reported by BJ007 from a slightly expanded dataset but over a larger wavelength range (+/-200 km/s). These measurements agree also with the (5+/-2)% absorption reported by Vidal-Madjar et al. (2004) over the whole Lyman-alpha line from independent, lower-resolution data. We show that stellar Lyman-alpha variability is unlikely to significantly affect those detections. The HI atoms must necessarily have velocities above the escape velocities and/or be outside the Roche lobe, given the lobe shape and orientation. Absorption by HI in HD209458b's atmosphere has thus been detected with different datasets, and now with independent analyses. All these results strengthen the concept of evaporating hot-Jupiters, as well as the modelization of this phenomenon.Comment: To be published in ApJ

Experimental Evidence for Quantum Interference and Vibrationally Induced Decoherence in Single-Molecule Junctions

We analyze quantum interference and decoherence effects in single-molecule junctions both experimentally and theoretically by means of the mechanically controlled break junction technique and density-functional theory. We consider the case where interference is provided by overlapping quasi-degenerate states. Decoherence mechanisms arising from the electronic-vibrational coupling strongly affect the electrical current flowing through a single-molecule contact and can be controlled by temperature variation. Our findings underline the all-important relevance of vibrations for understanding charge transport through molecular junctions.Comment: 5 pages, 4 figure

Directional cell movements downstream of Gbx2 and Otx2 control the assembly of sensory placodes

Cranial placodes contribute to sensory structures including the inner ear, the lens and olfactory epithelium and the neurons of the cranial sensory ganglia. At neurula stages, placode precursors are interspersed in the ectoderm surrounding the anterior neural plate before segregating into distinct placodes by as yet unknown mechanisms. Here, we perform live imaging to follow placode progenitors as they aggregate to form the lens and otic placodes. We find that while placode progenitors move with the same speed as their non-placodal neighbours, they exhibit increased persistence and directionality and these properties are required to assemble morphological placodes. Furthermore, we demonstrate that these factors are components of the transcriptional networks that coordinate placode cell behaviour including their directional movements. Together with previous work, our results support a dual role for Otx and Gbx transcription factors in both the early patterning of the neural plate border and the later segregation of its derivatives into distinct placodes

Precision radial velocities of double-lined spectroscopic binaries with an iodine absorption cell

A spectroscopic technique employing an iodine absorption cell (I_2) to superimpose a reference spectrum onto a stellar spectrum is currently the most widely adopted approach to obtain precision radial velocities of solar-type stars. It has been used to detect ~80 extrasolar planets out of ~130 know. Yet in its original version, it only allows us to measure precise radial velocities of single stars. In this paper, we present a novel method employing an I_2 absorption cell that enables us to accurately determine radial velocities of both components of double-lined binaries. Our preliminary results based on the data from the Keck I telescope and HIRES spectrograph demonstrate that 20-30 m/s radial velocity precision can be routinely obtained for "early" type binaries (F3-F8). For later type binaries, the precision reaches ~10 m/s. We discuss applications of the technique to stellar astronomy and searches for extrasolar planets in binary systems. In particular, we combine the interferometric data collected with the Palomar Testbed Interferometer with our preliminary precision velocities of the spectroscopic double-lined binary HD 4676 to demonstrate that with such a combination one can routinely obtain masses of the binary components accurate at least at the level of 1.0%.Comment: Accepted for publication in The Astrophysical Journa