Stationary waves and slowly moving features in the night upper clouds of Venus

At the cloud top level of Venus (65-70 km altitude) the atmosphere rotates 60 times faster than the underlying surface, a phenomenon known as superrotation. Whereas on Venus's dayside the cloud top motions are well determined and Venus general circulation models predict a mean zonal flow at the upper clouds similar on both day and nightside, the nightside circulation remains poorly studied except for the polar region. Here we report global measurements of the nightside circulation at the upper cloud level. We tracked individual features in thermal emission images at 3.8 and 5.0 $\mathrm{\mu m}$ obtained between 2006 and 2008 by the Visible and Infrared Thermal Imaging Spectrometer (VIRTIS-M) onboard Venus Express and in 2015 by ground-based measurements with the Medium-Resolution 0.8-5.5 Micron Spectrograph and Imager (SpeX) at the National Aeronautics and Space Administration Infrared Telescope Facility (NASA/IRTF). The zonal motions range from -110 to -60 m s$^{-1}$, consistent with those found for the dayside but with larger dispersion. Slow motions (-50 to -20 m s$^{-1}$) were also found and remain unexplained. In addition, abundant stationary wave patterns with zonal speeds from -10 to +10 m s$^{-1}$ dominate the night upper clouds and concentrate over the regions of higher surface elevation.Comment: 15 pages, 4 figures, 6 supplementary figure

Active Membrane Fluctuations Studied by Micropipet Aspiration

We present a detailed analysis of the micropipet experiments recently reported in J-B. Manneville et al., Phys. Rev. Lett. 82, 4356--4359 (1999), including a derivation of the expected behaviour of the membrane tension as a function of the areal strain in the case of an active membrane, i.e., containing a nonequilibrium noise source. We give a general expression, which takes into account the effect of active centers both directly on the membrane, and on the embedding fluid dynamics, keeping track of the coupling between the density of active centers and the membrane curvature. The data of the micropipet experiments are well reproduced by the new expressions. In particular, we show that a natural choice of the parameters quantifying the strength of the active noise explains both the large amplitude of the observed effects and its remarkable insensitivity to the active-center density in the investigated range. [Submitted to Phys Rev E, 22 March 2001]Comment: 14 pages, 5 encapsulated Postscript figure

Cross calibration between Hayabusa2/ONC-T and OSIRIS-REx/MapCam for comparative analyses between asteroids Ryugu and Bennu

Proximity observations of (162173) Ryugu by the telescopic Optical Navigation Camera onboard Hayabusa2 and (101955) Bennu by MapCam onboard Origins, Spectral Interpretation, Resource Identification, and Security-Regolith Explorer found opposite spectral trends of space weathering on these carbonaceous asteroids. Whether the space weathering trends on these asteroids evolved from the same starting spectra would place an important constraint for understanding their relation. However, systematic error between data obtained by the two imagers needed to be reduced for accurate comparison. To resolve this problem, we cross calibrated albedo and color data using the Moon as the common standard. We show that the cross-calibrated reflectance can be obtained by upscaling the pre-cross-calibrated reflectance of Bennu by 12 +/- 2% at v-band, reducing the systematic errors down to 2%. The cross-calibrated data show that Bennu is brighter by 16 +/- 2% at v-band and bluer in spectral slope by 0.19 +/- 0.05 (/um) than Ryugu. The spectra of fresh craters on Ryugu and Bennu before cross calibration appeared to follow two parallel trend lines with offset, but they converged to a single trend after cross calibration. Such a post-cross-calibration perspective raise the possibility that Ryugu and Bennu evolved from materials with similar visible spectra but evolved in diverging directions by space weathering. The divergent evolution can be caused by the difference in space weathering dose/process and/or composition of the starting material. Thus, comparing the composition of samples returned from Ryugu and Bennu may change the way we interpret the spectral variation of C-complex asteroids

Protein Conformational Changes in the Bacteriorhodopsin Photocycle: Comparison of Findings from Electron and X-Ray Crystallographic Analyses

Light-driven conformational changes in the membrane protein bacteriorhodopsin have been studied extensively using X-ray and electron crystallography, resulting in the deposition of >30 sets of coordinates describing structural changes at various stages of proton transport. Using projection difference Fourier maps, we show that coordinates reported by different groups for the same photocycle intermediates vary considerably in the extent and nature of conformational changes. The different structures reported for the same intermediate cannot be reconciled in terms of differing extents of change on a single conformational trajectory. New measurements of image phases obtained by cryo-electron microscopy of the D96G/F171C/F219L triple mutant provide independent validation for the description of the large protein conformational change derived at 3.2 Å resolution by electron crystallography of 2D crystals, but do not support atomic models for light-driven conformational changes derived using X-ray crystallography of 3D crystals. Our findings suggest that independent determination of phase information from 2D crystals can be an important tool for testing the accuracy of atomic models for membrane protein conformational changes

Dietary t10,c12-CLA but not c9,t11 CLA Reduces Adipocyte Size in the Absence of Changes in the Adipose Renin–Angiotensin System in fa/fa Zucker Rats

In obesity, increased activity of the local renin–angiotensin system (RAS) and enlarged adipocytes with altered adipokine production are linked to the development of obesity-related health problems and cardiovascular disease. Mixtures of conjugated linoleic acid (CLA) isomers have been shown to reduce adipocyte size and alter the production of adipokines. The objective of this study was to investigate the effects of feeding individual CLA isomers on adipocyte size and adipokines associated with the local adipose RAS. Male fa/fa Zucker rats received either (a) control, (b) cis(c)9,trans(t)11-CLA, or (c) t10,c12-CLA diet for 8 weeks. The t10,c12-CLA isomer reduced adipocyte size and increased cell number in epididymal adipose tissue. RT-PCR and Western blot analysis revealed that neither CLA isomer altered mRNA or protein levels of angiotensinogen or AngII receptors in adipose tissue. Likewise, levels of the pro-inflammatory cytokines TNF-α and IL-6 or the anti-inflammatory cytokine IL-10 were unchanged in adipose tissue. Similarly, neither CLA isomer had any effect on phosphorylation nor DNA binding of NF-κB. Our results suggest that although the t10,c12-CLA isomer had beneficial effects on reducing adipocyte size in obese rats, this did not translate into changes in the local adipose RAS or associated adipokines

Force-Velocity Measurements of a Few Growing Actin Filaments

The authors propose a new mechanism for actin-based force generation based on results using chains of actin-grafted magnetic colloids

Cucurbitacin I Inhibits Cell Motility by Indirectly Interfering with Actin Dynamics

Cucurbitacins are plant natural products that inhibit activation of the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway by an unknown mechanism. They are also known to cause changes in the organization of the actin cytoskeleton. actin depolymerization experiments, cucurbitacin I had no effect on the rate of actin filament disassembly at the nanomolar concentrations that inhibit cell migration. At elevated concentrations, the depolymerization rate was also unaffected, although there was a delay in the initiation of depolymerization. Therefore, cucurbitacin I targets some factor involved in cellular actin dynamics other than actin itself. Two candidate proteins that play roles in actin depolymerization are the actin-severing proteins cofilin and gelsolin. Cucurbitacin I possesses electrophilic reactivity that may lead to chemical modification of its target protein, as suggested by structure-activity relationship data. However, mass spectrometry revealed no evidence for modification of purified cofilin or gelsolin by cucurbitacin I.Cucurbitacin I results in accumulation of actin filaments in cells by a unique indirect mechanism. Furthermore, the proximal target of cucurbitacin I relevant to cell migration is unlikely to be the same one involved in activation of the JAK2/STAT3 pathway

An artificial impact on the asteroid (162173) Ryugu formed a crater in the gravity-dominated regime

The Hayabusa2 spacecraft investigated the small asteroid Ryugu, which has a rubble-pile structure. We describe an impact experiment on Ryugu using Hayabusa2’s Small Carry-on Impactor. The impact produced an artificial crater with a diameter >10 meters, which has a semicircular shape, an elevated rim, and a central pit. Images of the impact and resulting ejecta were recorded by the Deployable CAMera 3 for >8 minutes, showing the growth of an ejecta curtain (the outer edge of the ejecta) and deposition of ejecta onto the surface. The ejecta curtain was asymmetric and heterogeneous and it never fully detached from the surface. The crater formed in the gravity-dominated regime; in other words, crater growth was limited by gravity not surface strength. We discuss implications for Ryugu’s surface age

Two Distinct Mechanisms for Actin Capping Protein Regulation—Steric and Allosteric Inhibition

A crystallographic study reveals the structural basis for regulation by two different inhibitors of the actin capping protein, a critical factor controlling actin-driven cell motility