Scaling-Up: A Fifth Year of Restoring Oyster Reefs in Great Bay Estuary, NH 2013 Annual Program Report

The eastern oyster (Crassostrea virginica) in New Hampshire’s Great Bay Estuary has declined in the past decades, with local populations reduced due primarily to disease, excessive siltation, and past over-harvest. The loss of filtering oysters results in diminished ecological benefits for water quality, nitrogen control, and other services that healthy oyster populations provide. In support of management objectives to restore oyster populations, The Nature Conservancy (TNC) and the University of New Hampshire (UNH) have combined for a fifth consecutive year of scaled-up methods to rebuild oyster reefs and populations. Since 2009, we have “planted” seasoned shell, primarily surf-clam and oyster mix, on channel bottom as a hard substrate foundation to recruit spawn from nearby native populations. Constructed areas are amended to supplement recruitment with laboratory-raised and volunteer-grown “spat-on-shell” from remotely set larvae. Following four consecutive years of experience and adaptation, 2013 was a year of unprecedented effort and conservation outcomes. We successfully constructed and seeded five new acres of reef adjacent to native oysters in the Piscataqua River in Dover (1.5 acres) and in the Lamprey River in Newmarket (3.5 acres). Notably, we employed a new shell deployment method to achieve large-scale reef construction. Restoration efforts were greatly enhanced by excellent remote set success and outstanding natural recruitment, resulting in over 2M oysters. In addition, community engagement through the volunteer Oyster Conservationist program reached another all-time high with fifty families producing our largest oyster stock ever for restoration. Over the past five years, our efforts have added over 13 acres and 3M oysters to the ecosystem, increasing native Great Bay Estuary oyster populations by about 10%

What Missouri Thinks: An Examination of Public Attitudes

Political observers often refer to Missouri as a “bellwether” state, suggesting that the political and social attitudes of Missourians tend to reflect those of the rest of the United States. (Robertson, 2004). To better understand the opinions of Missourians on a set of important political, economic, and social issues, researchers at the Harry S Truman School of Public Affairs examined results from the 2008 Cooperative Congressional Election Survey (CCES). This nationally-representative public opinion survey included a sample of 817 Missouri residents. In this report, we explore the attitudes of Missourians to examine how they differ from the rest of the nation and how they vary within the state. Given the demographic patterns of Missouri, we are particularly interested in potential differences in attitudes between residents living in urban and rural areas (see Appendix A for additional discussion.) Overall, we find that Missouri residents shared similar attitudes on major economic, social and military issues with the rest of the nation, but we also find that rural and urban Missourians held distinctly different opinions on these same issues. These differences in opinion are likely driven by factors such as partisan identification, political ideology, education, and other socioeconomic attributes.Includes bibliographical reference

Novel Colicin F-Y of Yersinia frederiksenii Inhibits Pathogenic Yersinia Strains via YiuR-Mediated Reception, TonB Import, and Cell Membrane Pore Formation

A novel colicin type, designated colicin F-Y, was found to be encoded and produced by the strain Yersinia frederiksenii Y27601. Colicin F-Y was active against both pathogenic and nonpathogenic strains of the genus Yersinia. Plasmid YF27601 (5,574 bp) of Y. frederiksenii Y27601 was completely sequenced. The colicin F-Y activity gene (cfyA) and the colicin F-Y immunity gene (cfyI) were identified. The deduced amino acid sequence of colicin F-Y was very similar in its C-terminal pore-forming domain to colicin Ib (69% identity in the last 178 amino acid residues), indicating pore forming as its lethal mode of action. Transposon mutagenesis of the colicin F-Y-susceptible strain Yersinia kristensenii Y276 revealed the yiuR gene (ykris001_4440), which encodes the YiuR outer membrane protein with unknown function, as the colicin F-Y receptor molecule. Introduction of the yiuR gene into the colicin F-Y-resistant strain Y. kristensenii Y104 restored its susceptibility to colicin F-Y. In contrast, the colicin F-Y-resistant strain Escherichia coli TOP10F' acquired susceptibility to colicin F-Y only when both the yiuR and tonB genes from Y. kristensenii Y276 were introduced. Similarities between colicins F-Y and Ib, similarities between the Cir and YiuR receptors, and the detected partial cross-immunity of colicin F-Y and colicin Ib producers suggest a common evolutionary origin of the colicin F-Y-YiuR and colicin Ib-Cir systems

Concerted loop motion triggers induced fit of FepA to ferric enterobactin

Spectroscopic analyses of fluorophore-labeled Escherichia coli FepA described dynamic actions of its surface loops during binding and transport of ferric enterobactin (FeEnt). When FeEnt bound to fluoresceinated FepA, in living cells or outer membrane fragments, quenching of fluorophore emissions reflected conformational motion of the external vestibular loops. We reacted Cys sulfhydryls in seven surface loops (L2, L3, L4, L5, L7 L8, and L11) with fluorophore maleimides. The target residues had different accessibilities, and the labeled loops themselves showed variable extents of quenching and rates of motion during ligand binding. The vestibular loops closed around FeEnt in about a second, in the order L3 > L11 > L7 > L2 > L5 > L8 > L4. This sequence suggested that the loops bind the metal complex like the fingers of two hands closing on an object, by individually adsorbing to the iron chelate. Fluorescence from L3 followed a biphasic exponential decay as FeEnt bound, but fluorescence from all the other loops followed single exponential decay processes. After binding, the restoration of fluorescence intensity (from any of the labeled loops) mirrored cellular uptake that depleted FeEnt from solution. Fluorescence microscopic images also showed FeEnt transport, and demonstrated that ferric siderophore uptake uniformly occurs throughout outer membrane, including at the poles of the cells, despite the fact that TonB, its inner membrane transport partner, was not detectable at the poles

Vegetative Ecological Characteristics of Restored Reed (Phragmites australis) Wetlands in the Yellow River Delta, China

In this study, we compared ecological characteristics of wetland vegetation in a series of restoration projects that were carried out in the wetlands of Yellow River Delta. The investigated characteristics include plant composition structure, species diversity and community similarity in three kinds of Phragmites australis wetlands, i.e. restored P. australis wetlands (R1, R2, R3 and R4: restored in 2002, 2005, 2007 and 2009, respectively), natural P. australis wetland (N) and degraded P. australis wetland (D) to assess the process of wetlands restoration. The coverage of the R1 was 99%, which was similar to natural wetland. Among all studied wetlands, the highest and lowest stem density was observed in R1 and R2, respectively, Plant height and stem diameter show the same trend as N > R2 > R1 > R3 > D > R4. Species diversity of restored P. australis wetlands became closed to natural wetland. Both species richness and Shannon–Wiener index had similar tendency: increased first and then decreased with restored time. The highest species richness and species diversity were observed in R2, while the lowest values of those parameters were found in natural P. australis wetland. Similarity indexes between restored wetlands and natural wetland increased with the restoration time, but they were still less than 50%. The results indicate that the vegetation of P. australis wetlands has experienced a great improvement after several years’ restoration, and it is feasible to restored degraded P. australis wetlands by pouring fresh water into those wetlands in the Yellow River Delta. However, it is notable that costal degraded P. australis wetland in this region may take years to decades to reach the status of natural wetland

Management of Hazardous Waste and Contaminated Land

Regulation of hazardous waste and cleanup of contaminated sites are two major components of modern public policy for environmental protection. We review the literature on these related areas, with emphasis on empirical analyses. Researchers have identified many behavioral responses to regulation of hazardous waste, including changes in the location of economic activity. However, the drivers behind compliance with these costly regulations remain a puzzle, as most research suggests a limited role for conventional enforcement. Increasingly sophisticated research examines the benefits of cleanup of contaminated sites, yet controversy remains about whether the benefits of cleanup in the U.S. exceed its costs. Finally, research focusing on the imposition of legal liability for damages from hazardous waste finds advantages and disadvantages of the U.S. reliance on legal liability to pay for cleanup, as opposed to the government-financed approaches more common in Europe

Conservative Protestantism and skepticism of scientists studying climate change

Politicians who proclaim both their skepticism about global warming and their conservative religious credentials leave the impression that conservative Protestants may be more skeptical about scientists' claims regarding global warming than others. The history of the relationship between conservative Protestantism and science on issues such as evolution also suggests that there may be increased skepticism. Analyzing the 2006 and 2010 General Social Survey, we find no evidence that conservative Protestantism leads respondents to have less belief in the conclusiveness of climate scientists' claims. However, a second type of skepticism of climate scientists is an unwillingness to follow scientists' public policy recommendations. We find that conservative Protestantism does lead to being less likely to want environmental scientists to influence the public policy debate about what to do about climate change. Existing sociological research on the relationship between religion and science suggests that this stance is due to a long-standing social/moral competition between conservative Protestantism and science. © 2013 Springer Science+Business Media Dordrecht