A Complex Interaction Between Reduced Reelin Expression and Prenatal Organophosphate Exposure Alters Neuronal Cell Morphology.

Genetic and environmental factors are both likely to contribute to neurodevelopmental disorders including schizophrenia, autism spectrum disorders, and major depressive disorders. Prior studies from our laboratory and others have demonstrated that the combinatorial effect of two factors-reduced expression of reelin protein and prenatal exposure to the organophosphate pesticide chlorpyrifos oxon-gives rise to acute biochemical effects and to morphological and behavioral phenotypes in adolescent and young adult mice. In the current study, we examine the consequences of these factors on reelin protein expression and neuronal cell morphology in adult mice. While the cell populations that express reelin in the adult brain appear unchanged in location and distribution, the levels of full length and cleaved reelin protein show persistent reductions following prenatal exposure to chlorpyrifos oxon. Cell positioning and organization in the hippocampus and cerebellum are largely normal in animals with either reduced reelin expression or prenatal exposure to chlorpyrifos oxon, but cellular complexity and dendritic spine organization is altered, with a skewed distribution of immature dendritic spines in adult animals. Paradoxically, combinatorial exposure to both factors appears to generate a rescue of the dendritic spine phenotypes, similar to the mitigation of behavioral and morphological changes observed in our prior study. Together, our observations support an interaction between reelin expression and chlorpyrifos oxon exposure that is not simply additive, suggesting a complex interplay between genetic and environmental factors in regulating brain morphology

Design and implementation of a cost-aware and smart oyster mushroom cultivation system

Mushrooms are a nutritious food source, which can play a crucial role in providing affordable sources of proteins, vitamins and minerals for people worldwide, but their cultivation requires extensive training and considerable relevant expertise in order to fine-tune multiple environmental parameters. Internally displaced people in the Northern regions of Syria rely on very small-scale traditional oyster mushroom production, which cannot meet their local demand. Many international and local non-governmental organizations (NGOs) working for Syrian refugees, work on mushroom cultivation projects. They have reported significant difficulties and challenges in mushroom cultivation amongst the targeted beneficiaries. Therefore, the two main questions driving this research are: (1) How can organic mushroom cultivation be promoted using a robust and affordable intelligent mushroom farming system? (2) How can organic mushroom farming practices be simplified to support internally displaced and refugee Syrians? This research evaluates the process of automating mushroom cultivation by designing and implementing a smart oyster (Pleurotus ostreatus) mushroom farming system to remotely monitor and manage environmental parameters, such as temperature, humidity, air quality and illumination, inside the farm. Furthermore, ready and dedicated user-friendly web interfaces were also implemented to enable farmers to remotely monitor and manage their farms through the Internet. As a result, a dependable and cost-effective intelligent oyster mushroom cultivation system was designed and implemented in this work. The system includes remote monitoring and management via user-friendly interfaces. This simplifies mushroom cultivation for not only refugees and displaced communities, but also for mushroom farmers in low-income countries. This work can contribute to the eradication of poverty and hunger, in line with the United Nations Sustainable Development Goals one and two

Imaging-guided chest biopsies: techniques and clinical results

Background This article aims to comprehensively describe indications, contraindications, technical aspects, diagnostic accuracy and complications of percutaneous lung biopsy. Methods Imaging-guided biopsy currently represents one of the predominant methods for obtaining tissue specimens in patients with lung nodules; in many cases treatment protocols are based on histological information; thus, biopsy is frequently performed, when technically feasible, or in case other techniques (such as bronchoscopy with lavage) are inconclusive. Results Although a coaxial system is suitable in any case, two categories of needles can be used: fine-needle aspiration biopsy (FNAB) and core-needle biopsy (CNB), with the latter demonstrated to have a slightly higher overall sensitivity, specificity and accuracy. Conclusion Percutaneous lung biopsy is a safe procedure even though a few complications are possible: pneumothorax, pulmonary haemorrhage and haemoptysis are common complications, while air embolism and seeding are rare, but potentially fatal complications

Transplantation of canine olfactory ensheathing cells producing chondroitinase ABC promotes chondroitin sulphate proteoglycan digestion and axonal sprouting following spinal cord injury

Olfactory ensheathing cell (OEC) transplantation is a promising strategy for treating spinal cord injury (SCI), as has been demonstrated in experimental SCI models and naturally occurring SCI in dogs. However, the presence of chondroitin sulphate proteoglycans within the extracellular matrix of the glial scar can inhibit efficient axonal repair and limit the therapeutic potential of OECs. Here we have used lentiviral vectors to genetically modify canine OECs to continuously deliver mammalian chondroitinase ABC at the lesion site in order to degrade the inhibitory chondroitin sulphate proteoglycans in a rodent model of spinal cord injury. We demonstrate that these chondroitinase producing canine OECs survived at 4 weeks following transplantation into the spinal cord lesion and effectively digested chondroitin sulphate proteoglycans at the site of injury. There was evidence of sprouting within the corticospinal tract rostral to the lesion and an increase in the number of corticospinal axons caudal to the lesion, suggestive of axonal regeneration. Our results indicate that delivery of the chondroitinase enzyme can be achieved with the genetically modified OECs to increase axon growth following SCI. The combination of these two promising approaches is a potential strategy for promoting neural regeneration following SCI in veterinary practice and human patients

Human Astrocytes Exhibit Tumor Microenvironment-, Age-, and Sex-Related Transcriptomic Signatures

: Astrocytes are critical for the development and function of synapses. There are notable species differences between human astrocytes and commonly used animal models. Yet, it is unclear whether astrocytic genes involved in synaptic function are stable or exhibit dynamic changes associated with disease states and age in humans, which is a barrier in understanding human astrocyte biology and its potential involvement in neurological diseases. To better understand the properties of human astrocytes, we acutely purified astrocytes from the cerebral cortices of over 40 humans across various ages, sexes, and disease states. We performed RNA sequencing to generate transcriptomic profiles of these astrocytes and identified genes associated with these biological variables. We found that human astrocytes in tumor-surrounding regions downregulate genes involved in synaptic function and sensing of signals in the microenvironment, suggesting involvement of peri-tumor astrocytes in tumor-associated neural circuit dysfunction. In aging, we also found downregulation of synaptic regulators and upregulation of markers of cytokine signaling, while in maturation we identified changes in ionic transport with implications for calcium signaling. In addition, we identified subtle sexual dimorphism in human cortical astrocytes, which has implications for observed sex differences across many neurological disorders. Overall, genes involved in synaptic function exhibit dynamic changes in the peritumor microenvironment and aging. This data provides powerful new insights into human astrocyte biology in several biologically relevant states, that will aid in generating novel testable hypotheses about homeostatic and reactive astrocytes in humans.SIGNIFICANCE STATEMENTAstrocytes are an abundant class of cells playing integral roles at synapses. Astrocyte dysfunction is implicated in a variety of human neurological diseases. Yet our knowledge of astrocytes is largely based on mouse studies. Direct knowledge of human astrocyte biology remains limited. Here, we present transcriptomic profiles of human cortical astrocytes, and we identified molecular differences associated with age, sex, and disease state. We found that peritumor and aging astrocytes downregulate genes involved in astrocyte-synapse interactions. These data provide necessary insight into human astrocyte biology that will improve our understanding of human disease

Challenges for mobile remote access to desktop and web resources

Mobile remote access to personal computers and/or Web resources (i.e. Web contents and services) has the potential to enable important changes to the work practices of both businesses and individuals. There have been a number of attempts from both academic researchers as well as the industrial sector to develop mobile systems that can remotely access workstations or Web resources; however, a global acceptance of these systems has not been achieved yet. In this paper we review a decade of progress in the mobile remote access research area. We also highlight some of the open research issues and challenges that still exist. Copyright 2006 ACM

Mechanisms of olfactory ensheathing cell-enhanced neurite outgrowth and axon regeneration after spinal cord injury

Olfactory ensheathing cells (OECs) provide a pro-regenerative environment for the axons of olfactory receptor neurons and therefore are a promising candidate for cell transplantation therapy following spinal cord injury. We previously showed that OEC transplantation supports axon regeneration and functional re-connectivity following complete spinal cord injury, yet lack of an OEC-specific marker limited our ability to determine how they promoted these beneficial effects. Using both in vitro and in vivo models, we investigated the mechanisms by which OECs mediate axon regeneration. OECs enhance neurite outgrowth of postnatal cortical neurons in a scar-like culture model. We provide strong evidence that direct OEC-neurite alignment is critical to enhance neurite outgrowth in scar-like astrocyte and meningeal fibroblast inhibitory environments. We also tested eGFP-OECs from transgenic rats and showed that they facilitate neurite outgrowth in vitro. Then in a short-term study, we analyzed OEC survival, migration, and distribution within the lesion site of complete spinal cord transected rats. We found that rats transplanted with OECs preserve and associate with axons and neurons in the lesion core, reduce the presence of inhibitory CSPGs and myelin debris, and reduce secondary tissue damage due to microglial and macrophage activation and infiltration post-injury. Collectively, these data support a neuroprotective and proregenerative role of OECs through the modulation of glial scar formation following a complete spinal cord transection