Establishment and characterization of two human breast carcinoma cell lines by spontaneous immortalization: Discordance between Estrogen, Progesterone and HER2/neu receptors of breast carcinoma tissues with derived cell lines

Background: Breast cancer is one of the most common cancers among women throughout the world. Therefore, established cell lines are widely used as in vitro experimental models in cancer research.Methods: Two continuous human breast cell lines, designated MBC1 and MBC2, were successfully established and characterized from invasive ductal breast carcinoma tissues of Malaysian patients. MBC1 and MBC2 have been characterized in terms of morphology analysis, population doubling time, clonogenic formation, wound healing assay, invasion assay, cell cycle, DNA profiling, fluorescence immunocytochemistry, Western blotting and karyotyping.Results: MBC1 and MBC2 exhibited adherent monolayer epithelial morphology at a passage number of 150. Receptor status of MBC1 and MBC2 show (ER+, PR+, HER2+) and (ER+, PR-, HER2+), respectively. These results are in discordance with histopathological studies of the tumoral tissues, which were triple negative and (ER-, PR-, HER2+) for MBC1 and MBC2, respectively. Both cell lines were capable of growing in soft agar culture, which suggests their metastatic potential. The MBC1 and MBC2 metaphase spreads showed an abnormal karyotype, including hyperdiploidy and complex rearrangements with modes of 52-58 chromosomes per cell.Conclusions: Loss or gain in secondary properties, deregulation and specific genetic changes possibly conferred receptor changes during the culturing of tumoral cells. Thus, we hypothesize that, among heterogenous tumoral cells, only a small minority of ER+/PR+/HER2+ and ER+/PR-/HER2+ cells with lower energy metabolism might survive and adjust easily to in vitro conditions. These cell lines will pave the way for new perspectives in genetic and biological investigations, drug resistance and chemotherapy studies, and would serve as prototype models in Malaysian breast carcinogenesis investigations. © 2012 Kamalidehghan et al.; licensee BioMed Central Ltd

High-frequency Oscillations in Small Magnetic Elements Observed with Sunrise/SuFI

We characterize waves in small magnetic elements and investigate their propagation in the lower solar atmosphere from observations at high spatial and temporal resolution. We use the wavelet transform to analyze oscillations of both horizontal displacement and intensity in magnetic bright points found in the 300 nm and the Ca II H 396.8 nm passbands of the filter imager on board the Sunrise balloon-borne solar observatory. Phase differences between the oscillations at the two atmospheric layers corresponding to the two passbands reveal upward propagating waves at high frequencies (up to 30 mHz). Weak signatures of standing as well as downward propagating waves are also obtained. Both compressible and incompressible (kink) waves are found in the small-scale magnetic features. The two types of waves have different, though overlapping, period distributions. Two independent estimates give a height difference of approximately 450+-100 km between the two atmospheric layers sampled by the employed spectral bands. This value, together with the determined short travel times of the transverse and longitudinal waves provide us with phase speeds of 29+-2 km/s and 31+-2 km/s, respectively. We speculate that these phase speeds may not reflect the true propagation speeds of the waves. Thus, effects such as the refraction of fast longitudinal waves may contribute to an overestimate of the phase speed.Comment: 14 pages, 7 figure

Migration of Ca II H bright points in the internetwork

The migration of magnetic bright point-like features (MBP) in the lower solar atmosphere reflects the dispersal of magnetic flux as well as the horizontal flows of the atmospheric layer they are embedded in. We analyse trajectories of the proper motion of intrinsically magnetic, isolated internetwork Ca II H MBPs (mean lifetime 461 +- 9 s) to obtain their diffusivity behaviour. We use seeing-free high spatial and temporal resolution image sequences of quiet-Sun, disc-centre observations obtained in the Ca II H 3968 {\AA} passband of the Sunrise Filter Imager (SuFI) onboard the Sunrise balloon-borne solar observatory. Small MBPs in the internetwork are automatically tracked. The trajectory of each MBP is then calculated and described by a diffusion index ({\gamma}) and a diffusion coefficient (D). We further explore the distribution of the diffusion indices with the help of a Monte Carlo simulation. We find {\gamma} = 1.69 +- 0.08 and D = 257 +- 32 km^2/s averaged over all MBPs. Trajectories of most MBPs are classified as super-diffusive, i.e., {\gamma} > 1, with the determined {\gamma} being to our knowledge the largest obtained so far. A direct correlation between D and time-scale ({\tau}) determined from trajectories of all MBPs is also obtained. We discuss a simple scenario to explain the diffusivity of the observed, relatively short-lived MBPs while they migrate within a small area in a supergranule (i.e., an internetwork area). We show that the scatter in the {\gamma} values obtained for individual MBPs is due to their limited lifetimes. The super-diffusive MBPs can be well-described as random walkers (due to granular evolution and intergranular turbu- lence) superposed on a large systematic (background) velocity, caused by granular, mesogranular and supergranular flows.Comment: 10 pages, 7 figures, 3 table

Inclinations of small quiet-Sun magnetic features based on a new geometric approach

High levels of horizontal magnetic flux have been reported in the quiet-Sun internetwork, often based on Stokes profile inversions. Here we introduce a new method for deducing the inclination of magnetic elements and use it to test magnetic field inclinations from inversions. We determine accurate positions of a set of small, bright magnetic elements in high spatial resolution images sampling different photospheric heights obtained by the Sunrise balloon-borne solar observatory. Together with estimates of the formation heights of the employed spectral bands, these provide us with the inclinations of the magnetic features. We also compute the magnetic inclination angle of the same magnetic features from the inversion of simultaneously recorded Stokes parameters. Our new, geometric method returns nearly vertical fields (average inclination of around 14 deg with a relatively narrow distribution having a standard deviation of 6 deg). In strong contrast to this, the traditionally used inversions give almost horizontal fields (average inclination of 75+-8 deg) for the same small magnetic features, whose linearly polarised Stokes profiles are adversely affected by noise. The almost vertical field of bright magnetic features from our geometric method is clearly incompatible with the nearly horizontal magnetic fields obtained from the inversions. This indicates that the amount of magnetic flux in horizontal fields deduced from inversions is overestimated in the presence of weak Stokes signals, in particular if Stokes Q and U are close to or under the noise level. By combining the proposed method with inversions we are not just improving the inclination, but also the field strength. This technique allows us to analyse features that are not reliably treated by inversions, thus greatly extending our capability to study the complete magnetic field of the quiet Sun.Comment: 12 pages, 9 figures, 1 table; Accepted for publication in Astronomy & Astrophysic

Outpatient antibiotic prescription trends in the United States: A national cohort study

OBJECTIVETo characterize trends in outpatient antibiotic prescriptions in the United StatesDESIGNRetrospective ecological and temporal trend study evaluating outpatient antibiotic prescriptions from 2013 to 2015SETTINGNational administrative claims data from a pharmacy benefits manager PARTICIPANTS. Prescription pharmacy beneficiaries from Express Scripts Holding CompanyMEASUREMENTSAnnual and seasonal percent change in antibiotic prescriptionsRESULTSApproximately 98 million outpatient antibiotic prescriptions were filled by 39 million insurance beneficiaries during the 3-year study period. The most commonly prescribed antibiotics were azithromycin, amoxicillin, amoxicillin/clavulanate, ciprofloxacin, and cephalexin. No significant changes in individual or overall annual antibiotic prescribing rates were found during the study period. Significant seasonal variation was observed, with antibiotics being 42% more likely to be prescribed during February than September (peak-to-trough ratio [PTTR], 1.42; 95% confidence interval [CI], 1.39–1.61). Similar seasonal trends were found for azithromycin (PTTR, 2.46; 95% CI, 2.44–3.47), amoxicillin (PTTR, 1.52; 95% CI, 1.42–1.89), and amoxicillin/clavulanate (PTTR, 1.78; 95% CI, 1.68–2.29).CONCLUSIONSThis study demonstrates that annual national outpatient antibiotic prescribing practices remained unchanged during our study period. Furthermore, seasonal peaks in antibiotics generally used to treat viral upper respiratory tract infections remained unchanged during cold and influenza season. These results suggest that inappropriate prescribing of antibiotics remains widespread, despite the concurrent release of several guideline-based best practices intended to reduce inappropriate antibiotic consumption; however, further research linking national outpatient antibiotic prescriptions to associated medical conditions is needed to confirm these findings.Infect Control Hosp Epidemiol 2018;39:584–589</jats:sec

Study of the magnetostructural transition in critical-element free Mn1−xNi1−xFe2xSi0.95Al0.05

publishedVersio

Polarised kink waves in magnetic elements: evidence for chromospheric helical waves

In recent years, new high spatial resolution observations of the Sun's atmosphere have revealed the presence of a plethora of small-scale magnetic elements down to the resolution limit of the current cohort of solar telescopes (~100–120 km on the solar photosphere). These small magnetic field concentrations, due to the granular buffeting, can support and guide several magnetohydrodynamic wave modes that would eventually contribute to the energy budget of the upper layers of the atmosphere. In this work, exploiting the high spatial and temporal resolution chromospheric data acquired with the Swedish 1 m Solar Telescope, and applying the empirical mode decomposition technique to the tracking of the solar magnetic features, we analyze the perturbations of the horizontal velocity vector of a set of chromospheric magnetic elements. We find observational evidence that suggests a phase relation between the two components of the velocity vector itself, resulting in its helical motion

Climate change impact on bioclimatic deficiency, using microLEIS DSS in Ahar soils, Iran

Regional impact studies of the future climate change effects are necessary because projected changes in meteorological variables differ from one region to another, and different climate systems can react in varied ways to the same changes. In this study, the effects of climate change on bioclimatic deficiency were compared in two cultivation methods (irrigated and rainfed) in a semi-arid region, Ahar (East Azarbaijan, IRAN). The agricultural land uses selected for evaluation were wheat (Triticum aestivum), alfalfa (Medicago sativa), sugar beet (Beta vulgaris), potato (Solanum tuberosum), and maize (Zea mays). In this way, Terraza model included in the land evaluation decision support system, called MicroLEIS DSS, was used. Terraza gives a quantitative prediction of a site bioclimatic deficiency. Soil morphological and analytical data were obtained from 44 sampling points based on a grid survey. Agro-climatic data, referred to temperature and precipitation, were collected from weather stations located in Ahar region, which benefits from more than 20 consecutive years of weather data. A future scenario of climate change was calculated according to the Intergovernmental Panel on Climate Change (IPCC) on regions of Asia under scenario A1FI (highest future emission) for 2080s. Although, increasing of precipitation being available by climate change in the future scenario, humidity index will be reduced because of high temperature. The results showed that climate change is likely to cause severe water stress in irrigated cultivation of alfalfa, sugar beet, potato, and maize, the use of irrigation methods being essential to maintain agricultural productivity. Although irrigation is indicated as very important in this regime of semi-arid agriculture, cultivation of rainfed wheat can be possible instead of the irrigated one. Also, it is revealed that climate perturbation effects on rainfed conditions are more serious than those on the irrigated conditions in the area.The authors wish to thank Tabriz University for funding this research work, a dissertation of Ph. D. program undertaken by Farzin Shahbazi. They also thank Consejo Superior de Investigaciones Científicas (CSIC), Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS), Sevilla, Spain for their sincere cooperation during the candidate’ s sabbatical studies.Peer Reviewe

Kinematics of Magnetic Bright Features in the Solar Photosphere

Convective flows are known as the prime means of transporting magnetic fields on the solar surface. Thus, small magnetic structures are good tracers of the turbulent flows. We study the migration and dispersal of magnetic bright features (MBFs) in intergranular areas observed at high spatial resolution with Sunrise/IMaX. We describe the flux dispersal of individual MBFs as a diffusion process whose parameters are computed for various areas in the quiet Sun and the vicinity of active regions from seeing-free data. We find that magnetic concentrations are best described as random walkers close to network areas (diffusion index, gamma=1.0), travelers with constant speeds over a supergranule (gamma=1.9-2.0), and decelerating movers in the vicinity of flux emergence and/or within active regions (gamma=1.4-1.5). The three types of regions host MBFs with mean diffusion coefficients of 130 km^2/s, 80-90 km^2/s, and 25-70 km^2/s, respectively. The MBFs in these three types of regions are found to display a distinct kinematic behavior at a confidence level in excess of 95%.Comment: 8 pages, 4 figure