1,630 research outputs found

    Textbook of Melanoma

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    Field water dynamics in integrated systems in the Brazilian.

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    Agroforestry systems were established as a viable option for Brazilian farmers in recent decades. Shading is expected to affect the system?s microclimate and thus it is likely to alter water fluxes to the atmosphere via evapotranspiration. In this study we measured the evapotranspiration (ET) with micro-lysimeters in four different treatments as a proxy for different land use systems at EMBRAPA Beef Cattle, the Brazilian Agricultural Research Corporation, located in Campo Grande-MS, Brasil. The four treatments are: Integrated systems with rows of Eucalypt (Eucalyptus urograndis) trees (ICLF), integrated systems without Eucalypt trees (ICL), continuous pasture (CP) and native Cerrado (Savannah) vegetation. In the ICLF and ICL plots Bracchiaria brizantha was planted and in the CP plots Brachiaria decumbens. To measure the evapotranspiration lysimeters (diameter of 10 cm and a depth of 20 cm) were inserted into the ground and weighed daily during a period of 8 weeks. Within the ICLF systems, measurements were conducted in three different distances to the tree rows. In the treatments without trees the lysimeters were distributed randomly. Results were linked to data from the microclimate, i.e. wind speed, air humidity, and global radiation

    Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis

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    BACKGROUND: Epigenetic mechanisms regulate gene expression patterns affecting cell function and differentiation. In this report, we examine the role of histone acetylation in gene expression regulation in mouse embryonic stem cells employing transcriptomic and epigenetic analysis. RESULTS: Embryonic stem cells treated with the histone deacetylase inhibitor Trichostatin A (TSA), undergo morphological and gene expression changes indicative of differentiation. Gene profiling utilizing Affymetrix microarrays revealed the suppression of important pluripotency factors, including Nanog, a master regulator of stem cell identity, and the activation of differentiation-related genes. Transcriptional and epigenetic changes induced after 6-12 hours of TSA treatment mimic those that appear during embryoid body differentiation. We show here that the early steps of stem cell differentiation are marked by the enhancement of bulk activatory histone modifications. At the individual gene level, we found that transcriptional reprogramming triggered by histone deacetylase inhibition correlates with rapid changes in activating K4 trimethylation and repressive K27 trimethylation of histone H3. The establishment of H3K27 trimethylation is required for stable gene suppression whereas in its absence, genes can be reactivated upon TSA removal. CONCLUSION: Our data suggest that inhibition of histone deacetylases accelerates the early events of differentiation by regulating the expression of pluripotency- and differentiation-associated genes in an opposite manner. This analysis provides information about genes that are important for embryonic stem cell function and the epigenetic mechanisms that regulate their expression

    Factorial Design Experiment to Analyze the Response of a Luminescent Photoelastic Coating

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