Plant methods:putting the spotlight on technological innovation in the plant sciences.

Plant Methods is a new journal for plant biologists, specialising in the rapid publication of peer-reviewed articles with a focus on technological innovation in the plant sciences. The aim of Plant Methods is to stimulate the development and adoption of new and improved techniques and research tools in plant biology. We hope to promote more consistent standards in the plant sciences, and make readily accessible laboratory and computer-based research tools available to the whole community. This will be achieved by publishing Research articles, Methodology papers and Reviews using the BioMed Central Open Access publishing model. The journal is supported by a prestigious editorial board, whose members all recognise the importance of technological innovation as a driver for basic science

Microrheological Characterization of Collagen Systems: From Molecular Solutions to Fibrillar Gels

Collagen is the most abundant protein in the extracellular matrix (ECM), where its structural organization conveys mechanical information to cells. Using optical-tweezers-based microrheology, we investigated mechanical properties both of collagen molecules at a range of concentrations in acidic solution where fibrils cannot form and of gels of collagen fibrils formed at neutral pH, as well as the development of microscale mechanical heterogeneity during the self-assembly process. The frequency scaling of the complex shear modulus even at frequencies of ~10 kHz was not able to resolve the flexibility of collagen molecules in acidic solution. In these solutions, molecular interactions cause significant transient elasticity, as we observed for 5 mg/ml solutions at frequencies above ~200 Hz. We found the viscoelasticity of solutions of collagen molecules to be spatially homogeneous, in sharp contrast to the heterogeneity of self-assembled fibrillar collagen systems, whose elasticity varied by more than an order of magnitude and in power-law behavior at different locations within the sample. By probing changes in the complex shear modulus over 100-minute timescales as collagen self-assembled into fibrils, we conclude that microscale heterogeneity appears during early phases of fibrillar growth and continues to develop further during this growth phase. Experiments in which growing fibrils dislodge microspheres from an optical trap suggest that fibril growth is a force-generating process. These data contribute to understanding how heterogeneities develop during self-assembly, which in turn can help synthesis of new materials for cellular engineering

The worker branch in Yorkshire as a way of organising Polish migrants: exploring the process of carving out diasporic spaces within the trade union structure

While post-2004 Polish labour migration to the UK was underpinned by diasporic spaces instrumental in facilitating social and labour market adjustments, the institutions of the host society such as trade unions also sought to establish links with migrants. The analysis of interactions between UK unions and EU migrants focused on organising strategies and specific provisions such as English language learning. However, the discussion tended to ignore the impacts of diasporic influences, from ethnicity and native languages of migrants to the outcomes of migrant worker organising. Drawing on ethnographic and qualitative data, this paper discusses how Polishness, in its ethnic, historic and linguistic manifestations, has affected the internal dynamics of a migrant worker organisation created by a major UK trade union. The explicit acknowledgement of diasporic particularities of post-2004 Polish migrants not only enabled labour organising activities but also shaped the migrant worker organisation from within. The strength of diasporic influences on one hand and the chosen form of union organising on the other created conditions for the development of diasporic spaces within the institution of the host society

Effects of Finite and Discrete Sampling and Blur on Microrheology Experiments

The frequency-dependent viscous and elastic properties of fluids can be determined from measurements of the thermal fluctuations of a micron-sized particle trapped by optical tweezers. Finite bandwidth and other instrument limitations lead to systematic errors in measurement of the fluctuations. In this work, we numerically represented power spectra of bead position measurements as if collected by two different measurement devices: a quadrant photodiode, which measures the deflection of the trapping laser; and a high-speed camera, which images the trapped bead directly. We explored the effects of aliasing, camera blur, sampling frequency, and measurement time. By comparing the power spectrum, complex response function, and the complex shear modulus with the ideal values, we found that the viscous and elastic properties inferred from the data are affected by the instrument limitations of each device. We discuss how these systematic effects might affect experimental results from microrheology measurements and suggest approaches to reduce discrepancies

Ethical difficulties in clinical practice : experiences of European doctors

Background: Ethics support services are growing in Europe to help doctors in dealing with ethical difficulties. Currently, insufficient attention has been focused on the experiences of doctors who have faced ethical difficulties in these countries to provide an evidence base for the development of these services. Methods: A survey instrument was adapted to explore the types of ethical dilemma faced by European doctors, how they ranked the difficulty of these dilemmas, their satisfaction with the resolution of a recent ethically difficult case and the types of help they would consider useful. The questionnaire was translated and given to general internists in Norway, Switzerland, Italy and the UK. Results: Survey respondents (n = 656, response rate 43%) ranged in age from 28 to 82 years, and averaged 25 years in practice. Only a minority (17.6%) reported having access to ethics consultation in individual cases. The ethical difficulties most often reported as being encountered were uncertain or impaired decisionmaking capacity (94.8%), disagreement among caregivers (81.2%) and limitation of treatment at the end of life (79.3%). The frequency of most ethical difficulties varied among countries, as did the type of issue considered most difficult. The types of help most often identified as potentially useful were professional reassurance about the decision being correct (47.5%), someone capable of providing specific advice (41.1%), help in weighing outcomes (36%) and clarification of the issues (35.9%). Few of the types of help expected to be useful varied among countries. Conclusion: Cultural differences may indeed influence how doctors perceive ethical difficulties. The type of help needed, however, did not vary markedly. The general structure of ethics support services would not have to be radically altered to suit cultural variations among the surveyed countries

Impact of Optimally Minimizing Delay Times on Safety at Signalized Intersections in Urban Areas, Case Study: The City of Virginia Beach

Optimally minimizing delay times at signalized intersections can significantly improve both traffic flow and safety. However, most traffic flow optimizing tools do not measure the effect on safety. This study uses nonlinear programming (NLP) algorithms to optimally minimize delay times and employs both Safety performance functions (SPFs) and empirical Bayes (EB) before-after methodology to measure the impact on safety presented as a Crash Modification Factor (CMF). A crash modification factor (CMF) is a multiplicative factor used by transportation practitioners to compute the expected number of crashes at specific study site(s) after a countermeasure has been proposed or is implemented. Using 2013 traffic data from seventeen signalized intersections located in Virginia Beach, the results show that optimally minimizing intersection delay times can result in a safety improvement of approximately 26.46% that is a CMF of 0.735. This result is not conclusive, but the significance of the findings shows the need for further investigations and potential inclusion in the future editions of the Highway Safety Manual (HSM)