Cromatosis de los bovinos o vaca morada en Colombia (II parte).

Se resumen la reproducción experimental de cromatosis de clima cálido en bovinos, utilizando la planta mamei-tomatillo, y la reproducción de la cromatosis de clima frío en conejos con el mamei de tierra fría. Se presentan los análisis físico-químicos practicados a los pigmentos, las hipótesis sobre la patogénesis de la entidad, las medidas preventivas y las recomendaciones que deben ser tenidas en cuenta en las regiones donde se ha diagnosticado la enfermedad. A partir de tejidos y orina de animales afectados por cromatosis de clima cálido, se aisló un pigmento y por ensayo de ignición se obtuvo un residuo permanente de reacción alcalina. De la planta mamei-tomatillo se aisló un alcaloide que al exponerse a la luz del sol y al aire, se torna violáceo, por lo cual se cree pueda ser el responsable de producir la cromatosis. Con la planta mamei de tierra fría no se ha adelantado ningún estudio fitoquímico, como tampoco con tejidos y orina de animales afectados ni en experimentación. No existe posibilidad de que la cromatosis sea predisposición de tipo genético, ya que se presentan diferentes razas, edades y sexos, siendo siempre el común denominador la presencia de Bunchosia pseudonitida o Bunchosia armeniaca en las fincas problema. No existe tratamiento que por sí mismo prevenga o cure la enfermedadGanado de lehce-Ganadería lech

Analysis of colorectal cancers in British Bangladeshi identifies early onset, frequent mucinous histotype and a high prevalence of RBFOX1 deletion

PMCID: PMC3544714This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Non-cell-autonomous regulation of interneuron specification mediated by extracellular vesicles

Disruption in neurogenesis and neuronal migration can influence the assembly of cortical circuits, affecting the excitatory-inhibitory balance and resulting in neurodevelopmental and neuropsychiatric disorders. Using ventral cerebral organoids and dorsoventral cerebral assembloids with mutations in the extracellular matrix gene LGALS3BP, we show that extracellular vesicles released into the extracellular environment regulate the molecular differentiation of neurons, resulting in alterations in migratory dynamics. To investigate how extracellular vesicles affect neuronal specification and migration dynamics, we collected extracellular vesicles from ventral cerebral organoids carrying a mutation in LGALS3BP, previously identified in individuals with cortical malformations and neuropsychiatric disorders. These results revealed differences in protein composition and changes in dorsoventral patterning. Proteins associated with cell fate decision, neuronal migration, and extracellular matrix composition were altered in mutant extracellular vesicles. Moreover, we show that treatment with extracellular vesicles changes the transcriptomic profile in neural progenitor cells. Our results indicate that neuronal molecular differentiation can be influenced by extracellular vesicles

Point‑of‑care ultrasound in cardiorespiratory arrest (POCUS‑CA): narrative review article.

The POCUS-CA (Point-of-care ultrasound in cardiac arrest) is a diagnostic tool in the Intensive Care Unit and Emergency Department setting. The literature indicates that in the patient in a cardiorespiratory arrest it can provide information of the etiology of the arrest in patients with non-defibrillable rhythms, assess the quality of compressions during cardiopulmonary resuscitation (CPR), and define prognosis of survival according to specific findings and, thus, assist the clinician in decision-making during resuscitation. This narrative review of the literature aims to expose the usefulness of ultrasound in the setting of cardiorespiratory arrest as a tool that allows making a rapid diagnosis and making decisions about reversible causes of this entity. More studies are needed to support the evidence to make ultrasound part of the resuscitation algorithms. Teamwork during cardiopulmonary resuscitation and the inclusion of ultrasound in a multidisciplinary approach is important to achieve a favorable clinical outcomepost-print2391 K

Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III gene

Background: Nested PCRs based on the Plasmodium 18s-rRNA gene have been extensively used for human malaria diagnosis. However, they are not practical when large quantities of samples need to be processed, further there have been challenges in the performance and when interpreting results, especially when submicroscopic infections are analysed. Here the use of "direct PCR" was investigated with the aim of improving diagnosis in the malaria elimination era.\ud \ud Methods: The performance of the Plasmodium cytochrome oxidase III gene (COX-III) based novel malaria detection strategies (direct nested PCR and direct single PCR) were compared using a 18s-rRNA direct nested PCR as a reference tool. Evaluations were based on sensitivity, specificity and the ability to detect mixed infections using control blood spot samples and field collected blood samples with final species diagnosis confirmation by sequencing.\ud \ud Results: The COX-III direct PCR (limit of detection: 0.6–2 parasites/μL) was more sensitive than the 18s-rRNA direct nested PCR (limit of detection: 2–10 parasites/μL). The COX-III direct PCR identified all 21 positive controls (no mixed infections detected) while the 18s-rRNA direct nested PCR identified 18/21 (including four mixed infections). Different concentrations of simulated mixed infections (Plasmodium vivax and Plasmodium falciparum) suggest that the COX-III direct PCR detects only the predominant species. When the 18s-rRNA direct nested PCR was used to detect Plasmodium in field collected bloods spots (n = 3833), there was discrepancy in the results from the genus PCR (16 % positive) and the species-specific PCR (5 % positive). Further, a large portion of a subset of these positive samples (93 % for genus and 60 % for P. vivax), did not align with Plasmodium sequences. In contrast, the COX-III direct PCR clearly identified (single bands confirmed with sequencing) 2 % positive Plasmodium samples including P. vivax, P. falciparum, Plasmodium malariae and Plasmodium ovale wallikeri.\ud \ud Conclusions: The COX-III single direct PCR is an alternative method for accurate detection of Plasmodium microscopic and submicroscopic infections in humans, especially when a large number of samples require screening. This PCR does not require DNA isolation, is sensitive, quick, produces confident/clear results, identifies all the Plasmodium species infecting humans, and is cost-effective.\u

Ajedrez en grid con accesibilidad para todos.

Investigación Reflexiva de Grid Accesible para el Doctorado en Ingeniería del Software con la Universidad Pontificia de Salamanca sede Madrid, basado en la demanda de personas discapacitadas que requieren acceder a la información. Según La Organización Mundial de la Salud, más de 400 millones personas en el mundo son discapacitadas. Motivo de sensibilización para que los diseñadores de Sistemas de última generación como los Sistemas en Grid sean accesibles para todos, donde el Ajedrez, disciplina deportiva sirve como modelo para presentar los grandes beneficios de los más importantes conceptos Grid evitando volver los desarrollos en Sistemas mudos, sordos e inaccesibles

Ontologías, websemántica, metadatos para ver con los oídos, oir con los ojos, hablar para todos.

Investigación Reflexiva de Web Semántica para el Doctorado en Ingeniería del Software con la UPSAM, basándose en la demanda de personas discapacitadas que requieren acceder a la Web. Los diseñadores de ontologías en la Web Semántica deben construir páginas universales, evitando volver a ésta uda, sorda, inaccesible. Investigando definiciones formales sobre: Sistema de Información, Calidad, Estándares de WAI, WCAG, W3C, directrices para WEB 1,0 y WEB 2.0, investigaciones de SIDAR y normas legales sobre diseño de sistemas accesibles. Se observa la carencia en la Ingeniería WEB de: sensibilidad, conocimiento de directrices, legislaciones precisas sobre diseños con Calidad y accesibilidad