Bio-Photonic Sensing Cells over transparent substrates for anti-gestrinone antibodies biosensing

[EN] In a previous work we introduced the term Bio-Photonic Sensing Cells (BICELLs), referred to periodic networks of nano-pillar suitable for biosensing when are vertically interrogated. In this article, we demonstrate the biosensing capabilities of a type of micrometric size BICELLs made of SU-8 nano-pillars fabricated over transparent substrates. We verify the biochips functionality comparing the theoretical simulations with the experimental results when are optically interrogated in transmission. We also demonstrate a sensitivity enhancement by reducing the pitch among nano-pillars from 800 to 700. nm. Thus, the Limit of Detection achievable in these types of BICELLs is in the order of 64. pg/mL for 700. nm in pitch among nano-pillars in comparison with 292. pg/mL for 800. nm in pitch when are interrogated by Fourier Transform Visible and Infrared Spectrometry. The experiments exhibited a good reproducibility with a relative standard deviation of 0.29% measured within 8 days for a specific concentration. Finally, BICELLs functionality was tested in real conditions with unpurified rabbit serum for detecting anti-gestrinone antibodies, demonstrating the high performance of this type of BICELLs to detect specific antibodies having immobilized the suitable bioreceptors onto the sensing surface. © 2011 Elsevier B.V.This work is done within the support of the Spanish Ministry of Science and Innovation under project BIOPSIA (REF: TEC2008-06574). The authors thanks the Comunidad de Madrid and Universidad Politecncia de Madrid (Project BIO-VERSATIL, Ref CCG10-UPM/SEM-5096), Generalitat Valenciana (project ACOMP/2010/009 and PROMETEO 2010/008) and Dr Eva Brun for providing the rabbit serum containing polyclonal antibodies for gestrinone studies. F.J.O. is grateful to the Generalitat Valenciana for the postdoctoral grant included in the VALi + d 2010 Programme for Postdoctoral Researchers.Sanza, F.; Holgado, M.; Ortega Higueruelo, FJ.; Casquel Del Campo, R.; López-Romero, D.; Bañuls Polo, MJ.; Laguna, MF.... (2011). Bio-Photonic Sensing Cells over transparent substrates for anti-gestrinone antibodies biosensing. Biosensors and Bioelectronics. 26:4842-4847. https://doi.org/10.1016/j.bios.2011.06.010S484248472

Localized surface plasmon resonance nanochips with molecularly imprinted polymer coating for explosives sensing

Sensor elements based on localized surface plasmon resonance phenomenon in arrays of Au nanostructures on glass substrates (nanochips) with molecularly imprinted acrylamide copolymer coating have been proposed for explosives analogues sensing in liquid and vapor phase. Nanochips exhibited detection limits of 1 pM in aqueous solution and 0.1 ppm in gaseous state against 4-nitrophenol. Vapor phase sensing of 4-nitrotoluene, 1-nitronaphthalene and 5-nitroisoquinoline using the developed 4-nitrophenol-imprinted plasmonic nanochips demonstrated partially selective response with time to signal saturation starting from 2 minutes.</jats:p

Surface plasmon resonance spectroscopy and electrochemistry study of 4-nitro-1,2-phenylenediamine: A switchable redox polymer with nitro functional groups

10.1021/la053014xLangmuir2283929-3935LANG