1,288 research outputs found
An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes.
Mass spectrometry (MS) has become a crucial technique for the analysis of protein complexes. Native MS has traditionally examined protein subunit arrangements, while proteomics MS has focused on sequence identification. These two techniques are usually performed separately without taking advantage of the synergies between them. Here we describe the development of an integrated native MS and top-down proteomics method using Fourier-transform ion cyclotron resonance (FTICR) to analyse macromolecular protein complexes in a single experiment. We address previous concerns of employing FTICR MS to measure large macromolecular complexes by demonstrating the detection of complexes up to 1.8 MDa, and we demonstrate the efficacy of this technique for direct acquirement of sequence to higher-order structural information with several large complexes. We then summarize the unique functionalities of different activation/dissociation techniques. The platform expands the ability of MS to integrate proteomics and structural biology to provide insights into protein structure, function and regulation
The change of Fermi surface topology in Bi2Sr2CaCu2O8 with doping
We report the observation of a change in Fermi surface topology of
Bi2Sr2CaCu2O8 with doping. By collecting high statistics ARPES data from
moderately and highly overdoped samples and dividing the data by the Fermi
function, we answer a long standing question about the Fermi surface shape of
Bi2Sr2CaCu2O8 close to the (pi,0) point. For moderately overdoped samples
(Tc=80K) we find that both the bonding and antibonding sheets of the Fermi
surface are hole-like. However for a doping level corresponding to Tc=55K we
find that the antibonding sheet becomes electron-like. This change does not
directly affect the critical temperature and therefore the superconductivity.
However, since similar observations of the change of the topology of the Fermi
surface were observed in LSCO and Bi2Sr2Cu2O6, it appears to be a generic
feature of hole-doped superconductors. Because of bilayer splitting, though,
this doping value is considerably lower than that for the single layer
materials, which again argues that it is unrelated to Tc
Self-energy of a nodal fermion in a d-wave superconductor
We re-consider the self-energy of a nodal (Dirac) fermion in a 2D d-wave
superconductor. A conventional belief is that Im \Sigma (\omega, T) \sim max
(\omega^3, T^3). We show that \Sigma (\omega, k, T) for k along the nodal
direction is actually a complex function of \omega, T, and the deviation from
the mass shell. In particular, the second-order self-energy diverges at a
finite T when either \omega or k-k_F vanish. We show that the full summation of
infinite diagrammatic series recovers a finite result for \Sigma, but the full
ARPES spectral function is non-monotonic and has a kink whose location compared
to the mass shell differs qualitatively for spin-and charge-mediated
interactions.Comment: 4pp 3 eps figure
Evolution of the Fermi surface with carrier concentration in Bi_2Sr_2CaCu_2O_{8+\delta}
We show, by use of angle-resolved photoemission spectroscopy, that underdoped
Bi_2Sr_2CaCu_2O_{8+\delta} appears to have a large Fermi surface centered at
(\pi,\pi), even for samples with a T_c as low as 15 K. No clear evidence of a
Fermi surface pocket around (\pi/2,\pi/2) has been found. These conclusions are
based on a determination of the minimum gap locus in the pseudogap regime T_c <
T < T^*, which is found to coincide with the locus of gapless excitations in
momentum space (Fermi surface) determined above T^*. These results suggest that
the pseudogap is more likely of precursor pairing rather than magnetic origin.Comment: 4 pages, revtex, 4 postscript color figure
Destroying coherence in high temperature superconductors with current flow
The loss of single-particle coherence going from the superconducting state to
the normal state in underdoped cuprates is a dramatic effect that has yet to be
understood. Here, we address this issue by performing angle resolved
photoemission spectroscopy (ARPES) measurements in the presence of a transport
current. We find that the loss of coherence is associated with the development
of an onset in the resistance, in that well before the midpoint of the
transition is reached, the sharp peaks in the ARPES spectra are completely
suppressed. Since the resistance onset is a signature of phase fluctuations,
this implies that the loss of single-particle coherence is connected with the
loss of long-range phase coherence.Comment: 7 pages, 7 figure
Extending the generalized Chaplygin gas model by using geometrothermodynamics
We use the formalism of geometrothermodynamics (GTD) to derive fundamental
thermodynamic equations that are used to construct general relativistic
cosmological models. In particular, we show that the simplest possible
fundamental equation, which corresponds in GTD to a system with no internal
thermodynamic interaction, describes the different fluids of the standard model
of cosmology. In addition, a particular fundamental equation with internal
thermodynamic interaction is shown to generate a new cosmological model that
correctly describes the dark sector of the Universe and contains as a special
case the generalized Chaplygin gas model.Comment: 18 pages, 7 figures. Section added: Basics aspects of
geometrothermodynamic
The coherent {\it d}-wave superconducting gap in underdoped LaSrCuO as studied by angle-resolved photoemission
We present angle-resolved photoemission spectroscopy (ARPES) data on
moderately underdoped LaSrCuO at temperatures below and
above the superconducting transition temperature. Unlike previous studies of
this material, we observe sharp spectral peaks along the entire underlying
Fermi surface in the superconducting state. These peaks trace out an energy gap
that follows a simple {\it d}-wave form, with a maximum superconducting gap of
14 meV. Our results are consistent with a single gap picture for the cuprates.
Furthermore our data on the even more underdoped sample
LaSrCuO also show sharp spectral peaks, even at the
antinode, with a maximum superconducting gap of 26 meV.Comment: Accepted by Phys. Rev. Let
Hole Pockets in the Doped 2D Hubbard Model
The electronic momentum distribution of the two
dimensional Hubbard model is studied for different values of the coupling , electronic density , and temperature, using
quantum Monte Carlo techniques. A detailed analysis of the data on
clusters shows that features consistent with hole pockets at momenta appear as the system is doped away
from half-filling. Our results are consistent with recent experimental data for
the cuprates discussed by Aebi et al. (Phys. Rev. Lett. {\bf 72}, 2757 (1994)).
In the range of couplings studied, the depth of the pockets is maximum at , and it increases with decreasing temperature.
The apparent absence of hole pockets in previous numerical studies of this
model is explained.Comment: 11 pages, 4 postscript figures appended, RevTeX (version 3.0
- …