35,320 research outputs found
A 128K-bit CCD buffer memory system
A prototype system was implemented to demonstrate that CCD's can be applied advantageously to the problem of low power digital storage and particularly to the problem of interfacing widely varying data rates. 8K-bit CCD shift register memories were used to construct a feasibility model 128K-bit buffer memory system. Peak power dissipation during a data transfer is less than 7 W., while idle power is approximately 5.4 W. The system features automatic data input synchronization with the recirculating CCD memory block start address. Descriptions are provided of both the buffer memory system and a custom tester that was used to exercise the memory. The testing procedures and testing results are discussed. Suggestions are provided for further development with regards to the utilization of advanced versions of CCD memory devices to both simplified and expanded memory system applications
Vitrification and determination of the crystallization time scales of the bulk-metallic-glass-forming liquid Zr58.5Nb2.8Cu15.6Ni12.8Al10.3
The crystallization kinetics of Zr58.5Nb2.8Cu15.6Ni12.8Al10.3 were studied in an electrostatic levitation (ESL) apparatus. The measured critical cooling rate is 1.75 K/s. Zr58.5Nb2.8Cu15.6Ni12.8Al10.3 is the first bulk-metallic-glass-forming liquid that does not contain beryllium to be vitrified by purely radiative cooling in the ESL. Furthermore, the sluggish crystallization kinetics enable the determination of the time-temperature-transformation (TTT) diagram between the liquidus and the glass transition temperatures. The shortest time to reach crystallization in an isothermal experiment; i.e., the nose of the TTT diagram is 32 s. The nose of the TTT diagram is at 900 K and positioned about 200 K below the liquidus temperature
Expression, purification, crystallization and preliminary crystallographic analysis of chitinase A from Vibrio carchariae
Peeling properties of lightlike signals in General Relativity
The peeling properties of a lightlike signal propagating through a general
Bondi-Sachs vacuum spacetime and leaving behind another Bondi-Sachs vacuum
space-time are studied. We demonstrate that in general the peeling behavior is
the conventional one which is associated with a radiating isolated system and
that it becomes unconventional if the asymptotically flat space-times on either
side of the history of the light-like signal tend to flatness at future null
infinity faster than the general Bondi-Sachs space-time. This latter situation
occurs if, for example, the space-times in question are static Bondi-Sachs
space- times.Comment: 14 pages, LaTeX2
Tracking TCRĂź sequence clonotype expansions during antiviral therapy using high-throughput sequencing of the hypervariable region
To maintain a persistent infection viruses such as hepatitis C virus (HCV) employ a range of mechanisms that subvert protective T cell responses. The suppression of antigen-specific T cell responses by HCV hinders efforts to profile T cell responses during chronic infection and antiviral therapy. Conventional methods of detecting antigen-specific T cells utilize either antigen stimulation (e.g., ELISpot, proliferation assays, cytokine production) or antigen-loaded tetramer staining. This limits the ability to profile T cell responses during chronic infection due to suppressed effector function and the requirement for prior knowledge of antigenic viral peptide sequences. Recently, high-throughput sequencing (HTS) technologies have been developed for the analysis of T cell repertoires. In the present study, we have assessed the feasibility of HTS of the TCRβ complementarity determining region (CDR)3 to track T cell expansions in an antigen-independent manner. Using sequential blood samples from HCV-infected individuals undergoing antiviral therapy, we were able to measure the population frequencies of >35,000 TCRβ sequence clonotypes in each individual over the course of 12 weeks. TRBV/TRBJ gene segment usage varied markedly between individuals but remained relatively constant within individuals across the course of therapy. Despite this stable TRBV/TRBJ gene segment usage, a number of TCRβ sequence clonotypes showed dramatic changes in read frequency. These changes could not be linked to therapy outcomes in the present study; however, the TCRβ CDR3 sequences with the largest fold changes did include sequences with identical TRBV/TRBJ gene segment usage and high junction region homology to previously published CDR3 sequences from HCV-specific T cells targeting the HLA-B*0801-restricted 1395HSKKKCDEL1403 and HLA-A*0101-restricted 1435ATDALMTGY1443 epitopes. The pipeline developed in this proof of concept study provides a platform for the design of future experiments to accurately address the question of whether T cell responses contribute to SVR upon antiviral therapy. This pipeline represents a novel technique to analyze T cell dynamics in situations where conventional antigen-dependent methods are limited due to suppression of T cell functions and highly diverse antigenic sequences
Evaluation of be-38 percent al alloy final report, 27 jun. 1964 - 28 feb. 1965
Mechanical properties, microstructural features, and general metallurgical quality of beryllium- aluminum allo
The Gravitational Hamiltonian in the Presence of Non-Orthogonal Boundaries
This paper generalizes earlier work on Hamiltonian boundary terms by omitting
the requirement that the spacelike hypersurfaces intersect the
timelike boundary orthogonally. The expressions for the action and
Hamiltonian are calculated and the required subtraction of a background
contribution is discussed. The new features of a Hamiltonian formulation with
non-orthogonal boundaries are then illustrated in two examples.Comment: 23 pages, 1 figure, LaTeX. The action is altered to include a corner
term which results in a different value for the non-orthogonal term. An
additional appendix with Euclidean results is included. To appear in Class.
Quant. Gra
- …