961 research outputs found
The genetics of ray pattern variation in Caenorhabditis briggsae
BACKGROUND: How does intraspecific variation relate to macroevolutionary change in morphology? This question can be addressed in species in which derived characters are present but not fixed. In rhabditid nematodes, the arrangement of the nine bilateral pairs of peripheral sense organs (rays) in tails of males is often the most highly divergent character between species. The development of ray pattern involves inputs from hometic gene expression patterns, TGFÎČ signalling, Wnt signalling, and other genetic pathways. In Caenorhabditis briggsae, strain-specific variation in ray pattern has provided an entrĂ©e into the evolution of ray pattern. Some strains were fixed for a derived pattern. Other strains were more plastic and exhibited derived and ancestral patterns at equal frequencies. RESULTS: Recombinant inbred lines (RILs) constructed from crosses between the variant C. briggsae AF16 and HK104 strains exhibited a wide range of phenotypes including some that were more extreme than either parental strain. Transgressive segregation was significantly associated with allelic variation in the C. briggsae homolog of abdominal B, Cb-egl-5. At least two genes that affected different elements of ray pattern, ray position and ray fusion, were linked to a second gene, mip-1. Consistent with this, the segregation of ray position and ray fusion phenotypes were only partially correlated in the RILs. CONCLUSIONS: The evolution of ray pattern has involved allelic variation at multiple loci. Some of these loci impact the specification of ray identities and simultaneously affect multiple ray pattern elements. Others impact individual characters and are not constrained by covariance with other ray pattern elements. Among the genetic pathways that may be involved in ray pattern evolution is specification of anteroposterior positional information by homeotic genes
Observations of stem water storage in trees of opposing hydraulic strategies
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/116368/1/ecs2201569165.pd
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Histologic and Morphometric Evaluation of Testes of Feral Tom Kittens and Cats
Background: Even with numerous successful Trap-Neuter-Release programs, feral cat populations continue to grow. Our laboratory is interested in determining if an underlying biological cause exists for the exuberant reproductive success in this once domestic subspecies. An earlier age for developing reproductive capacity (onset of spermatogenesis in males) may be one factor. For domestic toms, puberty is reported to occur around 8 months of age. Previous work by our laboratory has shown that normal morphologic sperm are present in vas deferens secretions of feral toms before 6 months of age. Therefore, our hypothesis was that in feral toms the onset of spermatogenesis occurred before 6 months of age. The study objective was to histologically evaluate testes from weanling (2 months of age) through adulthood (24 months of age) to determine when the onset of spermatogenesis occurs in feral toms.
Methods: Feral toms were presented for castration at a local Humane Society during August-October 2014. Age was determined by records provided from feral cat colony managers and confirmed with dental eruption patterns. The age groups were: 2-2.5 months (weanling; n=6), 3-4 months (juvenile; n=6), 5-6 months (pubertal; n=6), and 12-24 months (adult; n=6). General anesthesia was induced and a routine open castration was performed. Both testicles from each cat were hemi-sectioned, formalin-fixed, paraffin-embedded, cut into sections (6 ”m), and stained with hematoxylin and eosin. The slides were evaluated by a single observer (EB) blinded to age group using bright field microscopy (200X). Evidence of spermatogenesis was determined on the basis of presence of spermatozoa in the seminiferous tubule lumen. In addition, perpendicular diameters measured from 5 tubules for each testis were averaged and mean ± SD was determined for each age group. Tubular diameter was compared using a Studentâs t test where p < 0.05 was defined as significant. The presence of spermatozoa in the lumen was compared using a Chi-square test.
Results: Evidence of spermatogenesis in weanling, juvenile, pubertal, and adult toms was 0%, 17%, 67%, and 100%, respectively (p < 0.05 between successive age groups). The seminiferous tubular diameter was significantly larger in each successive age group (weanlings 88.10 ± 10.88 ”m; juveniles 109.8 ± 8.89 ”m; pubertal 142.2 ± 16.89 ”m; adult 237.90 ± 52.45 ”m).
Discussion: Juvenile feral toms in the current study had significantly wider tubular diameter than previously reported for domestic toms under 5 months of age (86 ”m), which supports our hypothesis that spermatogenesis is occurring at an earlier age in ferals. However, the time of year the previous measurements in domestics was not reported. If these measurements were made outside of the breeding season, this may explain why the tubular diameters were smaller. Future studies are planned to determine if folliculogenesis occurs earlier in queens as well
Investigation of the feasibility to use Zeeman-effect background correction for the graphite furnace determination of phosphorus using high-resolution continuum source atomic absorption spectrometry as a diagnostic tool
The determination of phosphorus by graphite furnace atomic absorption spectrometry at the non-resonance line at 213.6 nm, and the capability of Zeeman-effect background correction (Z-BC) to deal with the fine-structured background absorption due to the PO molecule have been investigated in the presence of selected chemical modifiers. Two line source atomic absorption spectrometers, one with a longitudinally heated and the other with a transversely heated graphite tube atomizer have been used in this study, as well as two prototype high-resolution continuum source atomic absorption spectrometers, one of which had a longitudinally arranged magnet at the furnace. It has been found that Z-BC is capable correcting very well the background caused by the PO molecule, and also that of the NO molecule, which has been encountered when the Pd + Ca mixed modifier was used. Both spectra exhibited some Zeeman splitting, which, however, did not cause any artifacts or correction errors. The practical significance of this study is to confirm that accurate results can be obtained for the determination of phosphorus using Z-BC.
The best sensitivity with a characteristic mass of m(0) = 11 ng P has been obtained with the pure Pd modifier, which also caused the lowest background level. The characteristic mass obtained with the mixed Pd + Ca modifier depended on the equipment used and was between m(0) = 9 ng P and m(0) = 15 ng P, and the background signal was higher. The major problem of Z-BC remains the relatively restricted linear working range
Active Disk Building in a local HI-Massive LIRG: The Synergy between Gas, Dust, and Star Formation
HIZOA J0836-43 is the most HI-massive (M_HI = 7.5x10^10 Msun) galaxy detected
in the HIPASS volume and lies optically hidden behind the Milky Way. Markedly
different from other extreme HI disks in the local universe, it is a luminous
infrared galaxy (LIRG) with an actively star forming disk (>50 kpc), central to
its ~ 130 kpc gas disk, with a total star formation rate (SFR) of ~20.5 Msun
yr^{-1}. Spitzer spectroscopy reveals an unusual combination of powerful
polycyclic aromatic hydrocarbon (PAH) emission coupled to a relatively weak
warm dust continuum, suggesting photodissociation region (PDR)-dominated
emission. Compared to a typical LIRG with similar total infrared luminosity
(L_TIR=10^11 Lsun), the PAHs in HIZOA J0836-43 are more than twice as strong,
whereas the warm dust continuum (lambda > 20micron) is best fit by a star
forming galaxy with L_TIR=10^10 Lsun. Mopra CO observations suggest an extended
molecular gas component (H_2 + He > 3.7x10^9 Msun) and a lower limit of ~ 64%
for the gas mass fraction; this is above average compared to local disk
systems, but similar to that of z~1.5 BzK galaxies (~57%). However, the star
formation efficiency (SFE = L_IR/L'_CO) for HIZOA J0836-43 of 140 Lsun (K km
s^{-1} pc^2)^{-1} is similar to that of local spirals and other disk galaxies
at high redshift, in strong contrast to the increased SFE seen in merging and
strongly interacting systems. HIZOA J0836-43 is actively forming stars and
building a massive stellar disk. Its evolutionary phase of star formation
(M_stellar, SFR, gas fraction) compared to more distant systems suggests that
it would be considered typical at redshift z~1. This galaxy provides a rare
opportunity in the nearby universe for studying (at z~0.036) how disks were
building and galaxies evolving at z~1, when similarly large gas fractions were
likely more common.Comment: Accepted for publication in The Astrophysical Journal. 16 pages, 8
figure
Civilian casualties and public support for military action: Experimental evidence
In contrast to the expansive literature on military casualties and support for war, we know very little about public reactions to foreign civilian casualties. This article, based on representative sample surveys in the US and Britain, reports four survey experiments weaving information about civilian casualties into vignettes about Western military action. These produce consistent evidence of civilian casualty aversion: where death tolls were higher, support for force was invariably and significantly lower. Casualty effects were moderate in size but robust across our two cases and across different scenarios. They were also strikingly resistant to moderation by other factors manipulated in the experiments, such as the framing of casualties or their religious affiliation. The importance of numbers over even strongly humanizing frames points towards a utilitarian rather than a social-psychological model of casualty aversion. Either way, civilian casualties deserve a more prominent place in the literature on public support for war
Speciesâspecific transpiration responses to intermediate disturbance in a northern hardwood forest
Intermediate disturbances shape forest structure and composition, which may in turn alter carbon, nitrogen, and water cycling. We used a largeâscale experiment in a forest in northern lower Michigan where we prescribed an intermediate disturbance by stem girdling all canopyâdominant early successional trees to simulate an accelerated ageârelated senescence associated with natural succession. Using 3 years of eddy covariance and sap flux measurements in the disturbed area and an adjacent control plot, we analyzed disturbanceâinduced changes to plot level and speciesâspecific transpiration and stomatal conductance. We found transpiration to be ~15% lower in disturbed plots than in unmanipulated control plots. However, speciesâspecific responses to changes in microclimate varied. While red oak and white pine showed increases in stomatal conductance during postdisturbance (62.5 and 132.2%, respectively), red maple reduced stomatal conductance by 36.8%. We used the hysteresis between sap flux and vapor pressure deficit to quantify diurnal hydraulic stress incurred by each species in both plots. Red oak, a ring porous anisohydric species, demonstrated the largest mean relative hysteresis, while red maple, bigtooth aspen, and paper birch, all diffuse porous species, had the lowest relative hysteresis. We employed the PenmanâMonteith model for LE to demonstrate that these speciesâspecific responses to disturbance are not well captured using current modeling strategies and that accounting for changes to leaf area index and plot microclimate are insufficient to fully describe the effects of disturbance on transpiration.Key PointsPlot level scaling of evaporation from sap flux evaluated with eddy fluxDisturbance changes intradaily transpiration dynamicsHydraulic strategy causes speciesâspecific transpiration differencesPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/110637/1/jgrg20315.pd
Characterizing the diurnal patterns of errors in the prediction of evapotranspiration by several landâsurface models: An NACP analysis
Landâsurface models use different formulations of stomatal conductance and plant hydraulics, and it is unclear which type of model best matches the observed surfaceâatmosphere water flux. We use the North American Carbon Program data set of latent heat flux (LE) measurements from 25 sites and predictions from 9 models to evaluate models' ability to resolve subdaily dynamics of transpiration. Despite overall good forecast at the seasonal scale, the models have difficulty resolving the dynamics of intradaily hysteresis. The majority of models tend to underestimate LE in the prenoon hours and overestimate in the evening. We hypothesize that this is a result of unresolved afternoon stomatal closure due to hydrodynamic stresses. Although no model or stomata parameterization was consistently best or worst in terms of ability to predict LE, errors in modelâsimulated LE were consistently largest and most variable when soil moisture was moderate and vapor pressure deficit was moderate to limiting. Nearly all models demonstrate a tendency to underestimate the degree of maximum hysteresis which, across all sites studied, is most pronounced during moistureâlimited conditions. These diurnal error patterns are consistent with models' diminished ability to accurately simulate the natural hysteresis of transpiration. We propose that the lack of representation of plant hydrodynamics is, in part, responsible for these error patterns. Key Points Landâsurface models produce subdaily patterns of latent heat flux error Error patterns are characterized by the stomatal conductance formulation used Current models lack a mechanism to simulate hysteretic transpirationPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/108341/1/jgrg20246.pd
IQ-Station: A Low Cost Portable Immersive Environment
The emergence of inexpensive 3D TVâs, affordable input and rendering hardware and open-source software has created a yeasty atmosphere for the development of low-cost immersive environments (IE). A low cost IE system, or IQ-station, fashioned from commercial off the shelf technology (COTS), coupled with a targeted immersive application can be a viable laboratory instrument for enhancing scientific workflow for exploration and analysis. The use of an IQ-station in a laboratory setting also has the potential of quickening the adoption of a more sophisticated immersive environment as a critical enabler in modern scientific and engineering workflows. Prior work in immersive environments generally required either a head mounted display (HMD) system or a large projector-based implementation both of which have limitations in terms of cost, usability, or space requirements. The solution presented here provides an alternative platform providing a reasonable immersive experience that addresses those limitations. Our work brings together the needed hardware and software to create a fully integrated immersive display and interface system that can be readily deployed in laboratories and common workspaces. By doing so, it is now feasible for immersive technologies to be included in researchersâ day-to-day workflows. The IQ-Station sets the stage for much wider adoption of immersive environments outside the small communities of virtual reality centers
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