Functional properties of neurons derived from in vitro reprogrammed postnatal astroglia

With the exception of astroglia-like cells in the neurogenic niches of the telencephalic subependymal or hippocampal subgranular zone, astroglia in all other regions of the adult mouse brain do not normally generate neurons. Previous studies have shown, however, that early postnatal cortical astroglia in culture can be reprogrammed to adopt a neuronal fate after forced expression of Pax6, a transcription factor (TF) required for proper neuronal specification during embryonic corticogenesis. Here we show that also the proneural genes neurogenin-2 and Mash1 (mammalian achaete schute homolog 1) possess the ability to reprogram astroglial cells from early postnatal cerebral cortex. By means of time-lapse imaging of green fluorescent astroglia, we provide direct evidence that it is indeed cells with astroglial characteristics that give rise to neurons. Using patch-clamp recordings in culture, we show that astroglia-derived neurons acquire active conductances and are capable of firing action potentials, thus displaying hallmarks of true neurons. However, independent of the TF used for reprogramming, astroglia-derived neurons appear to mature more slowly compared with embryonic-born neurons and fail to generate a functional presynaptic output within the culturing period. However, when cocultured with embryonic cortical neurons, astroglia-derived neurons receive synaptic input, demonstrating that they are competent of establishing a functional postsynaptic compartment. Our data demonstrate that single TFs are capable of inducing a remarkable functional reprogramming of astroglia toward a truly neuronal identity

Tension dynamics and viscoelasticity of extensible wormlike chains

The dynamic response of prestressed semiflexible biopolymers is characterized by the propagation and relaxation of tension, which arises due to the near inextensibility of a stiff backbone. It is coupled to the dynamics of contour length stored in thermal undulations, but also to the local relaxation of elongational strain. We present a systematic theory of tension dynamics for stiff yet extensible wormlike chains. Our results show that even moderate prestress gives rise to distinct Rouse-like extensibility signatures in the high-frequency viscoelastic response.Comment: 4 pages, 1 figure; corrected typo

Analysis of Simulated and Experimental Noise Sources of Boeing 777 Main Gear Model via CLEAN in 3D

Previously obtained experimental and simulated phased microphone array data for the NASA 26%-scale model of a six-wheel main landing gear, with and without a toboggan-shaped noise reduction fairing, were analyzed using the CLEAN technique in three dimensions. The model is a high-fidelity representation of the Boeing 777-200 aircraft main landing gear. The reprocessing of the acoustic data was undertaken to address the deficiencies encountered in the source localization (beamform) maps and the integrated farfield spectra at mid to high frequencies in our previous study, where conventional array processing techniques were applied to the same data sets. Application of the CLEAN approach resulted in higher quality beamform maps with many of the previously observed side lobes either eliminated or suppressed significantly. As a result, integration of the experimentally and computationally based maps showed substantial improvement in the quality of the high-frequency spectral content relative to the corresponding spectra generated via conventional array processing, thus extending the good agreement achieved between the measured and predicted farfield spectra from 8 kHz to 14 kHz in model-scale frequencies. In addition, an examination of the simulated surface pressure fluctuations, off-surface flow field, and three-dimensionally constructed noise sources was conducted to determine the cause of the subtle discrepancies between measured and predicted sound pressure levels that occur in the 1,800 Hz to 4,000 Hz model-scale frequency range

Argon metastable dynamics in a filamentary jet micro-discharge at atmospheric pressure

Space and time resolved concentrations of Ar ($^{3}P_2$) metastable atoms at the exit of an atmospheric pressure radio-frequency micro-plasma jet were measured using tunable diode laser absorption spectroscopy. The discharge features a coaxial geometry with a hollow capillary as an inner electrode and a ceramic tube with metal ring as outer electrode. Absorption profiles of metastable atoms as well as optical emission measurements reveal the dynamics and the filamentary structure of the discharge. The average spatial distribution of Ar metastables is characterized with and without a target in front of the jet, showing that the target potential and therewith the electric field distribution substantially changes the filaments' expansion. Together with the detailed analysis of the ignition phase and the discharge's behavior under pulsed operation, the results give an insight into the excitation and de-excitation mechanisms

Investigation of site-specific wind field parameters and their effect on loads of offshore wind turbines

The main contributing factors to unsteady loading of Offshore Wind Turbines (OWT) are wind shear, turbulence, and waves. In the present paper, the turbulence intensity and the wind shear exponent are investigated. Using data from the FINO 1 research platform, these parameters are analyzed and compared with the proposed wind field parameters in the IEC standard 61400-3. Based on this analysis, aeroelastic simulations are performed to determine the effect of wind field parameters on the fatigue and the extreme loads on the rotor blades. For the investigations, the aeroelastic model of a 5 MW OWT is used with a focus on design load cases in an operating state (power production). The fatigue loads are examinedby means of the damage-equivalent load-range approach. In order to determine the extreme loads with a recurrence period of 50 years, a peak over threshold extrapolation method and a novel method based on average conditional exceedance ratesare used. The results show that the requirements of the IEC standard are very conservative for the design of the rotor blades. Therefore, there could be a large optimization potential for the reduction of weight and cost of the rotor blades.Ministry for Science and Culture in Lower SaxonyFederal Maritime and Hydrographic Agency (BSH)Federal Ministry for the Environment, Nature Conservation and Nuclear Safety (BMU

How to Get Better: Taking Notes Mediates the Effect of a Video Tutorial on Number Series

In recent studies, test-score increases have been shown in rule-based intelligence test tasks, such as number series, after watching a corresponding video tutorial. An open question remains regarding the mechanisms involved. Specifically, taking notes to describe the relations between numbers might be linked to test scores, and is hypothesized to mediate the effect of a number series video tutorial on number series test scores. Therefore, an experimental group (EG) watching a number series video tutorial (n = 58) was compared with a control group (CG) watching an irrelevant tutorial (n = 52) before working on number series items. Results revealed higher number series scores in the EG than the CG (d = .48), more items with provided notes in the EG than in the CG (d = .41), and substantial correlations between the number of items with notes and the number series sum scores in both groups (EG: r = .66; CG: r = .75). The effect of the video tutorial on the number series sum score was mediated by the number of items with notes (indirect effect = 3.41, SE = 1.74). Theoretical and practical implications as well as future research directions are discussed

Two-electron wavefunctions are matrix product states with bond dimension Three

We prove the statement in the title, for a suitable (wavefunction-dependent) choice of the underlying orbitals, and show that Three is optimal. Thus for two-electron systems, the QC-DMRG method with bond dimension Three combined with fermionic mode optimization exactly recovers the FCI energy

Pion scattering in Wilson ChPT

We compute the scattering amplitude for pion scattering in Wilson chiral perturbation theory for two degenerate quark flavors. We consider two different regimes where the quark mass m is of order (i) a\Lambda_QCD^2 and (ii) a^2\Lambda_QCD^3. Analytic expressions for the scattering lengths in all three isospin channels are given. As a result of the O(a^2) terms the I=0 and I=2 scattering lengths do not vanish in the chiral limit. Moreover, additional chiral logarithms proportional to a^2\ln M_{\pi}^2 are present in the one-loop results for regime (ii). These contributions significantly modify the familiar results from continuum chiral perturbation theory.Comment: 20 pages, 4 figures. V3: Comments on finite size effects and the axial vector current added, one more reference. To be published in PR