prototypical implementations

In this technical report, we present prototypical implementations of innovative tools and methods developed according to the working plan outlined in Technical Report TR-B-09-05 [23]. We present an ontology modularization and integration framework and the SVoNt server, the server-side end of an SVN- based versioning system for ontologies in the Corporate Ontology Engineering pillar. For the Corporate Semantic Collaboration pillar, we present the prototypical implementation of a light-weight ontology editor for non-experts and an ontology based expert finder system. For the Corporate Semantic Search pillar, we present a prototype for algorithmic extraction of relations in folksonomies, a tool for trend detection using a semantic analyzer, a tool for automatic classification of web documents using Hidden Markov models, a personalized semantic recommender for multimedia content, and a semantic search assistant developed in co-operation with the Museumsportal Berlin. The prototypes complete the next milestone on the path to an integral Cor- porate Semantic Web architecture based on the three pillars Corporate Ontol- ogy Engineering, Corporate Semantic Collaboration, and Corporate Semantic Search, as envisioned in [23]

Extending the Automation Pyramid for Industrial Demand Response

Industrial demand response uses a multitude of energy flexibility measures. Their planning and control requires various production IT systems. A widely accepted approach to classify these inhouse IT systems are the levels of the automation pyramid in companies. This paper broadens the scope of this concept to overcome the limitation to companies’ (virtual) borders by including required IT systems that refine and monetarize a company’s energy flexibility, e.g. energy markets, aggregators, etc. Therefore, a holistic approach for the classification of functionalities for industrial demand response across companies and energy markets is developed and applied exemplarily

Garden and landscape-scale correlates of moths of differing conservation status: significant effects of urbanization and habitat diversity

Moths are abundant and ubiquitous in vegetated terrestrial environments and are pollinators, important herbivores of wild plants, and food for birds, bats and rodents. In recent years, many once abundant and widespread species have shown sharp declines that have been cited by some as indicative of a widespread insect biodiversity crisis. Likely causes of these declines include agricultural intensification, light pollution, climate change, and urbanization; however, the real underlying cause(s) is still open to conjecture. We used data collected from the citizen science Garden Moth Scheme (GMS) to explore the spatial association between the abundance of 195 widespread British species of moth, and garden habitat and landscape features, to see if spatial habitat and landscape associations varied for species of differing conservation status. We found that associations with habitat and landscape composition were species-specific, but that there were consistent trends in species richness and total moth abundance. Gardens with more diverse and extensive microhabitats were associated with higher species richness and moth abundance; gardens near to the coast were associated with higher richness and moth abundance; and gardens in more urbanized locations were associated with lower species richness and moth abundance. The same trends were also found for species classified as increasing, declining and vulnerable under IUCN (World Conservation Union) criteria

Design of a cyber-physical energy laboratory

Complex power supply structures are increasingly the target of cyber attacks and potentially very attractive for attackers. The constantly increasing networking in the course of decentralization makes attack vectors even larger. In order to discover potential security gaps in supply structures and to estimate the potential impact, a comprehensive test environment is required. A holistic structure of network, control and field level is presented, which has sufficient depth of detail to scientifically investigate various scenarios and to investigate the behavior of real field and control components

Local anomaly detection analysis in distribution grid based on IEC 61850-9-2 LE SV voltage signals

This paper introduces an approach of local voltage anomaly detection in electric power distribution grid using IEC 61850-9-2 LE Sampled Values (SV) as transmission protocol [1]. The approach was implemented and tested in a real-time simulation unit, where a defined distribution grid provides simulated voltage data. An analysis of transmitted samples on a receiver and computation device according to power quality measurements and their limits due to dynamic behaviour is done. The goal of our proposed anomaly detection is in contrast to previous approaches to detect disturbances caused by cyberattacks on ICS components in distribution grids based on an open communication interface. This paper defines the first part of detection at a local node in a defined grid configuration

A cross-domain physical testbed environment for cybersecurity performance evaluations

PCAPs of the paper "CrossTest: A cross-domain physical testbed environment for cybersecurity performance evaluations"

Visualization and Analysis of Hepatitis C Virus Structural Proteins at Lipid Droplets by Super-Resolution Microscopy

<div><p>Cytosolic lipid droplets are central organelles in the Hepatitis C Virus (HCV) life cycle. The viral capsid protein core localizes to lipid droplets and initiates the production of viral particles at lipid droplet–associated ER membranes. Core is thought to encapsidate newly synthesized viral RNA and, through interaction with the two envelope proteins E1 and E2, bud into the ER lumen. Here, we visualized the spatial distribution of HCV structural proteins core and E2 in vicinity of small lipid droplets by three-color 3D super-resolution microscopy. We observed and analyzed small areas of colocalization between the two structural proteins in HCV-infected cells with a diameter of approximately 100 nm that might represent putative viral assembly sites.</p></div

3D reconstitution of core, Flag-E2, and lipid droplets in JFH1^Flag-E2-infected cells.

<p>Representative 3D reconstructions of core and Flag-E2 spatial localization and colocalization around lipid droplets. Shown are three examples of JFH1^Flag-E2-infected cells, first the image stacks used for the 3D reconstruction and the 3D reconstruction of all three channels, then core or Flag-E2 and the lipid droplet volume and finally volume of colocalization between core and Flag-E2 and the lipid droplet volume. Scale bars on the image stacks and ruler hatch marks in the 3D images are 100 nm.</p

Visualization of lipid droplets in dSTORM.

<p>(<b>A</b>) Huh7 Lunet cells were incubated with LipidTox Red neutral lipid stain and analyzed by dSTORM. Time-dependent switch between “ON” and “OFF” states of LipidTox Red, shown is the signal of a dSTORM time series. (<b>B</b>) Huh7 Lunet cells were incubated with LipidTox Red neutral lipid stain and analyzed by widefield microscopy and dSTORM. Single channels are shown in black and white; the merged image is pseudocolored with widefield in white and dSTORM in blue (scale bar 5 µm, in magnified images 0.5 µm). (<b>C</b>) dSTORM of Huh7 Lunet cells after immunostaining with antibodies directed against endogenous ADRP/perilipin 2 and staining of lipid droplets with LipidTox red. Single channels are shown in black and white; the merged image is pseudocolored with ADRP in red and LipidTox in blue (scale bar 5 µm, in magnified images 0.5 µm).</p