The need to implement nonindustry COVID-19 clinical trials in non-high-income countries

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The effect of some processing conditions on the characteristics of biodegradable microspheres obtained by an emulsion solvent evaporation process

Unloaded microspheres were prepared from polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-valerate (PHB-HV) polymers using an oil-in-water emulsion solvent evaporation method. The study was conducted to evaluate how the polymer and some process parameters affect properties of the final microspheres such as particle size, superficial area, zeta potential, surface morphology and microsphere degradation. The variables included surfactant concentration in the emulsion water phase and solvent composition. From the results, it was found that the parameters affecting microsphere size the most were surfactant concentration in the emulsion water phase and solvent composition. Properties such as zeta potential, surface area and surface morphology remained pratically unchanged over the range of the processing conditions studied here.011

Environmental benchmarks based on ecotoxicological assessment with planktonic species might not adequately protect benthic assemblages in lotic systems

Freshwater ecosystems face widespread diffuse and point-source contamination. Species Sensitivity Distributions (SSDs) have been used as a tool to determine chemical concentration benchmarks that represent protective levels for most species in the environment. Here we used a SSD approach to assess on the adequacy of standard planktonic organisms to reflect the response of benthic communities, critically supporting the structure and function of lotic ecosystems. For the purpose, SSDs reflecting non-lethal responses of standard planktonic and selected benthic organisms were built based on EC50 values (collected in the literature or estimated following testing herein) regarding three model contaminants: potassium dichromate (PD), 3,5-dichlorophenol (DCP) and lead chloride (LC). The derived HC5 estimates were discriminatory between chemicals and the uncertainty associated with the estimate was remarkably low. The HC5 estimates with corresponding uncertainty were generally within the same order of magnitude for the three chemicals tested, with better discrimination between chemicals regarding their hazardous potential being achieved for benthic organisms: DCP was clearly less hazardous than PD, but LC tends to be as hazardous as PD and DCP (assuming the confidence interval ranges). Moreover, benthic communities were more sensitive to both DCP and PD, in this later case the HC5 being lower by more than one order of magnitude than that found for planktonic communities; for LC, confidence intervals overlapped, preventing a feasible assumption regarding differential sensitivity of the compared communities. Microphytobenthos was highlighted as the most sensitive group to the three tested chemicals in SSDs covering the benthic compartment, while SSDs with planktonic organisms did not consistently show trends in sensitivity ordering. Overall, our results suggest that protective benchmarks retrieved from SSDs built with the responses of standard planktonic organisms (which are the most commonly used for regulation purposes) do not adequately protect benthic communities.publishe

Impact of a winter upwelling event on the distribution and transport of sardine (Sardina pilchardus) eggs and larvae off western Iberia: a retention mechanism.

A hydrographic and ichthyoplanktonic survey was conducted on the western Iberia Peninsula shelf-slope, with the objective of assessing the influence of wind-driven flow on the transport and survival of sardine early life history stages. Measurements were taken during a 2-week period of an 'upwelling-favourable' wind event. An offshore displacement of the surface low salinity Western Iberia Buoyant Plume (WIBP) was observed well off the shelf break. Below the plume, the current was southward over the mid- and inner-shelf. Offshore, the flow was poleward in the northern part of the survey area and equatorward in the south. A convergence zone was detected along the outer shelf and upper slope where the buoyant plume was apparently trapped and deepened. In general, eggs and larvae were distributed according to the spreading of the low salinity surface waters, and also within the convergent band. Older larvae were retained within the latter feature. The role of wind forcing on the plume and its interaction with the slope flow were analysed. A mechanism of larvae retention was formulated based on simplified models of the observed shelf-slope circulation