Peptide hydrogels — a tissue engineering strategy for the prevention of oesophageal strictures

Endoscopic treatment of Barrett’s oesophagus often leads to further damage of healthy tissue causing fibrotic tissue formation termed as strictures. This study shows that synthetic, self-assembling peptide hydrogels (PeptiGelDesign) support the activity and function of primary oesophageal cells, leading to epithelialisation and stratification during in vitro 3D co-culture. Following buffering in culture media, oesophageal stromal fibroblasts (rOSFs) were incorporated into a library of peptide hydrogels, whereas oesophageal epithelial cells (mOECs) were seeded on the surface. Optimal hydrogels (PGD-AlphaProC and PGD-CGD2) supported mOEC viability (>95 %), typical cell morphology (cobblestone-like), a migration rate of 17.4 μm/hr and a migration distance of 364 μm, at 48 hours. Positive expression of typical epithelial markers (ZO-1 and cytokeratins) was witnessed detected using immunocytochemistry at day 3 in culture. Furthermore, optimal hydrogels were identified which supported rOSF viability (> 95%) with homogenous distribution when incorporated into the hydrogels and also promoted the secretion of collagen type I detected using ELISA, at day 7. 3D co-culture model using optimal hydrogels for both cell types supported a stratified epithelial layer (expressing involucrin and AE1/AE3 markers). Findings from this study could lead to the use of peptide hydrogels as a minimally invasive endoscopic therapy to manage oesophageal strictures

Towards screening Barrett’s Oesophagus: current guidelines, imaging modalities and future developments

Barrett’s oesophagus is the only known precursor to oesophageal adenocarcinoma (OAC). Although guidelines on the screening and surveillance exist in Barrett’s oesophagus, the current strategies are inadequate. Oesophagogastroduodenoscopy (OGD) is the gold standard method in screening for Barrett’s oesophagus. This invasive method is expensive with associated risks negating its use as a current screening tool for Barrett’s oesophagus. This review explores current definitions, epidemiology, biomarkers, surveillance, and screening in Barrett’s oesophagus. Imaging modalities applicable to this condition are discussed, in addition to future developments. There is an urgent need for an alternative non-invasive method of screening and/or surveillance which could be highly beneficial towards reducing waiting times, alleviating patient fears and reducing future costs in current healthcare services. Vibrational spectroscopy has been shown to be promising in categorising Barrett’s oesophagus through to high-grade dysplasia (HGD) and OAC. These techniques need further validation through multicentre trials

Toward a better understanding of the cultural identity negotiation: The experiences of minority youth in multicultural societies

How do immigrant and minority youth navigate between and within their heritage culture and the majority culture of the wider society? Acculturation theory and research point to a widespread preference for integration (adopting and maintaining bicultural affiliations) and a strong link between integration and psychological wellbeing. Despite compelling empirical evidence about the psychological and social benefits of integration, there is limited research about how immigrant and minority youth go about achieving it. This thesis examines the mechanisms underpinning cultural identity integration, how immigrant and minority youth select desirable aspects of both cultures and blend them together in a novel way (hybridising) or shift between and across cultural identities depending on situational factors (alternating). The research is based on the core model developed by Ward and associates, which demonstrates that a motivation to integrate activates both hybrid and alternating identity styles but that these styles lead to different cultural identity outcomes (consolidation versus conflict) and have divergent effects on wellbeing (Ward, Ng Tseung-Wong, Szabo, Qumseya & Bhowon, 2018). The thesis has three main objectives: 1) to test the core mediational model of cultural identity negotiation with other minority groups and in other cultural contexts; 2) to extend the model by exploring how socio-political factors and family dynamics affect cultural identity styles and their outcomes; and 3) to explore gaps and omissions in the model to guide future research. To these ends, mixed methods are used across three studies with Arab youth in New Zealand and Arab-Palestinian youth in the state of Israel. The first study tested the mediational model of cultural identity negotiation quantitatively with Arab minority youth in New Zealand and expanded Ward et al.’s (2018) model by testing contextual variables as antecedents of the hybrid and alternating cultural identity styles and their outcomes. Subsequently, the second study uses the same methodology to investigate the experiences of young Palestinian citizens of Israel. The core mediational model was replicated in both contexts. Findings from both studies revealed that family context exerted similar influences on cultural identity styles while socio-political factors affected cultural identity styles and wellbeing in different ways. In addition, the alternating identity style appeared to be more responsive to contextual factors (family dynamics and socio-political context) than the hybrid identity style. The third study aimed to gain a broader and deeper understanding of the findings from the previous two studies, using qualitative methods to draw on the cross-cultural insights derived from a comparison of the New Zealand and Israel contexts. The qualitative study brought the individual agency into the spotlight as participants discussed selecting different strategies across work, home and university settings. When young people described their lived experiences, the alternating identity style emerged as a beneficial strategy enabling them to bridge cultural contexts without negative outcomes. Youth often had access to more than one cultural identity style and proactively interchanged them resiliently and flexibly to navigate a wide range of social environments. The combination of studies in this thesis provided additional insights into acculturation literature, integration and cultural identity styles. The findings of the research programme have contributed to gaining novel perspectives in understanding youth experiences during acculturation. More specifically, findings of this thesis led to greater understanding of what contextual factors influence the bicultural interplay of ethnic and civic participation and identity among multicultural youth. The mixed method design also has significant contributions that enabled a contextually situated understanding of the experiences of Arab youth within their cultural and socio-ecological environments and their experiences as minorities in two very different country contexts

TOWARD A BETTER UNDERSTANDING OF THE CULTURAL IDENTITY NEGOTIATION: THE EXPERIENCES OF MINORITY YOUTH IN MULTICULTURAL SOCIETIES

How do immigrant and minority youth navigate between and within their heritage culture and the majority culture of the wider society? Acculturation theory and research point to a widespread preference for integration (adopting and maintaining bicultural affiliations) and a strong link between integration and psychological wellbeing. Despite compelling empirical evidence about the psychological and social benefits of integration, there is limited research about how immigrant and minority youth go about achieving it. This thesis examines the mechanisms underpinning cultural identity integration, how immigrant and minority youth select desirable aspects of both cultures and blend them together in a novel way (hybridising) or shift between and across cultural identities depending on situational factors (alternating). The research is based on the core model developed by Ward and associates, which demonstrates that a motivation to integrate activates both hybrid and alternating identity styles but that these styles lead to different cultural identity outcomes (consolidation versus conflict) and have divergent effects on wellbeing (Ward, Ng Tseung-Wong, Szabo, Qumseya & Bhowon, 2018). The thesis has three main objectives: 1) to test the core mediational model of cultural identity negotiation with other minority groups and in other cultural contexts; 2) to extend the model by exploring how socio-political factors and family dynamics affect cultural identity styles and their outcomes; and 3) to explore gaps and omissions in the model to guide future research. To these ends, mixed methods are used across three studies with Arab youth in New Zealand and Arab-Palestinian youth in the state of Israel. The first study tested the mediational model of cultural identity negotiation quantitatively with Arab minority youth in New Zealand and expanded Ward et al.’s (2018) model by testing contextual variables as antecedents of the hybrid and alternating cultural identity styles and their outcomes. Subsequently, the second study uses the same methodology to investigate the experiences of young Palestinian citizens of Israel. The core mediational model was replicated in both contexts. Findings from both studies revealed that family context exerted similar influences on cultural identity styles while socio-political factors affected cultural identity styles and wellbeing in different ways. In addition, the alternating identity style appeared to be more responsive to contextual factors (family dynamics and socio-political context) than the hybrid identity style. The third study aimed to gain a broader and deeper understanding of the findings from the previous two studies, using qualitative methods to draw on the cross-cultural insights derived from a comparison of the New Zealand and Israel contexts. The qualitative study brought the individual agency into the spotlight as participants discussed selecting different strategies across work, home and university settings. When young people described their lived experiences, the alternating identity style emerged as a beneficial strategy enabling them to bridge cultural contexts without negative outcomes. Youth often had access to more than one cultural identity style and proactively interchanged them resiliently and flexibly to navigate a wide range of social environments. The combination of studies in this thesis provided additional insights into acculturation literature, integration and cultural identity styles. The findings of the research programme have contributed to gaining novel perspectives in understanding youth experiences during acculturation. More specifically, findings of this thesis led to greater understanding of what contextual factors influence the bicultural interplay of ethnic and civic participation and identity among multicultural youth. The mixed method design also has significant contributions that enabled a contextually situated understanding of the experiences of Arab youth within their cultural and socio-ecological environments and their experiences as minorities in two very different country contexts.</p

TOWARD A BETTER UNDERSTANDING OF THE CULTURAL IDENTITY NEGOTIATION: THE EXPERIENCES OF MINORITY YOUTH IN MULTICULTURAL SOCIETIES

How do immigrant and minority youth navigate between and within their heritage culture and the majority culture of the wider society? Acculturation theory and research point to a widespread preference for integration (adopting and maintaining bicultural affiliations) and a strong link between integration and psychological wellbeing. Despite compelling empirical evidence about the psychological and social benefits of integration, there is limited research about how immigrant and minority youth go about achieving it. This thesis examines the mechanisms underpinning cultural identity integration, how immigrant and minority youth select desirable aspects of both cultures and blend them together in a novel way (hybridising) or shift between and across cultural identities depending on situational factors (alternating). The research is based on the core model developed by Ward and associates, which demonstrates that a motivation to integrate activates both hybrid and alternating identity styles but that these styles lead to different cultural identity outcomes (consolidation versus conflict) and have divergent effects on wellbeing (Ward, Ng Tseung-Wong, Szabo, Qumseya & Bhowon, 2018). The thesis has three main objectives: 1) to test the core mediational model of cultural identity negotiation with other minority groups and in other cultural contexts; 2) to extend the model by exploring how socio-political factors and family dynamics affect cultural identity styles and their outcomes; and 3) to explore gaps and omissions in the model to guide future research. To these ends, mixed methods are used across three studies with Arab youth in New Zealand and Arab-Palestinian youth in the state of Israel. The first study tested the mediational model of cultural identity negotiation quantitatively with Arab minority youth in New Zealand and expanded Ward et al.’s (2018) model by testing contextual variables as antecedents of the hybrid and alternating cultural identity styles and their outcomes. Subsequently, the second study uses the same methodology to investigate the experiences of young Palestinian citizens of Israel. The core mediational model was replicated in both contexts. Findings from both studies revealed that family context exerted similar influences on cultural identity styles while socio-political factors affected cultural identity styles and wellbeing in different ways. In addition, the alternating identity style appeared to be more responsive to contextual factors (family dynamics and socio-political context) than the hybrid identity style. The third study aimed to gain a broader and deeper understanding of the findings from the previous two studies, using qualitative methods to draw on the cross-cultural insights derived from a comparison of the New Zealand and Israel contexts. The qualitative study brought the individual agency into the spotlight as participants discussed selecting different strategies across work, home and university settings. When young people described their lived experiences, the alternating identity style emerged as a beneficial strategy enabling them to bridge cultural contexts without negative outcomes. Youth often had access to more than one cultural identity style and proactively interchanged them resiliently and flexibly to navigate a wide range of social environments. The combination of studies in this thesis provided additional insights into acculturation literature, integration and cultural identity styles. The findings of the research programme have contributed to gaining novel perspectives in understanding youth experiences during acculturation. More specifically, findings of this thesis led to greater understanding of what contextual factors influence the bicultural interplay of ethnic and civic participation and identity among multicultural youth. The mixed method design also has significant contributions that enabled a contextually situated understanding of the experiences of Arab youth within their cultural and socio-ecological environments and their experiences as minorities in two very different country contexts