Features of financing of small business and microenterprises

In this article, we examined the specifics of financing small businesses and microenterprises

SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic leukemia-derived cell lines

<p>Abstract</p> <p>Background</p> <p><it>SET-NUP214 </it>fusion resulting from a recurrent cryptic deletion, del(9)(q34.11q34.13) has recently been described in T-cell acute lymphoblastic leukemia (T-ALL) and in one case of acute myeloid leukemia (AML). The fusion protein appears to promote elevated expression of <it>HOXA </it>cluster genes in T-ALL and may contribute to the pathogenesis of the disease. We screened a panel of ALL and AML cell lines for <it>SET-NUP214 </it>expression to find model systems that might help to elucidate the cellular function of this fusion gene.</p> <p>Results</p> <p>Of 141 human leukemia/lymphoma cell lines tested, only the T-ALL cell line LOUCY and the AML cell line MEGAL expressed the <it>SET(TAF-</it>Iβ)-<it>NUP214 </it>fusion gene transcript. RT-PCR analysis specifically recognizing the alternative first exons of the two <it>TAF-</it>I isoforms revealed that the cell lines also expressed <it>TAF-</it>Iα-<it>NUP214 </it>mRNA. Results of fluorescence in situ hybridization (FISH) and array-based copy number analysis were both consistent with del(9)(q34.11q34.13) as described. Quantitative genomic PCR also confirmed loss of genomic material between <it>SET </it>and <it>NUP214 </it>in both cell lines. Genomic sequencing localized the breakpoints of the <it>SET </it>gene to regions downstream of the stop codon and to <it>NUP214 </it>intron 17/18 in both LOUCY and MEGAL cells. Both cell lines expressed the 140 kDa SET-NUP214 fusion protein.</p> <p>Conclusion</p> <p>Cell lines LOUCY and MEGAL express the recently described <it>SET-NUP214 </it>fusion gene. Of special note is that the formation of the <it>SET </it>exon 7/<it>NUP214 </it>exon 18 gene transcript requires alternative splicing as the <it>SET </it>breakpoint is located downstream of the stop codon in exon 8. The cell lines are promising model systems for <it>SET-NUP214 </it>studies and should facilitate investigating cellular functions of the the SET-NUP214 protein.</p

Low-Energy \Lambda-\p Scattering Parameters from the pp→pK+Λpp \to pK^+\Lambda Reaction

Constraints on the spin-averaged $\Lambda p$ scattering length and effective range have been obtained from measurements of the $pp\to pK^+\Lambda$ reaction close to the production threshold by comparing model phase-space Dalitz plot occupations with experimental ones. The data fix well the position of the virtual bound state in the $\Lambda p$ system. Combining this with information from elastic $\Lambda p$ scattering measurements at slightly higher energies, together with the fact that the hyperdeuteron is not bound, leads to a new determination of the low energy $\Lambda p$ scattering parameters.Comment: 18 pages, 7 figure

Heavy Meson Production at COSY - 11

The COSY-11 collaboration has measured the total cross section for the pp --> pp eta-prime and pp --> pp eta reactions in the excess energy range from Q = 1.5 MeV to Q = 23.6 MeV and from Q = 0.5 MeV to Q = 5.4 MeV, respectively. Measurements have been performed with the total luminosity of 73 nb^(-1) for the pp --> pp eta reaction and 1360 nb^(-1) for the pp --> pp eta-prime one. Recent results are presented and discussed.Comment: Invited talk at 4th International Conference on Physics at Storage Rings (STORI 99), Bloomington, Indiana, USA, September 12-16, 199

Monitoring of the accelerator beam distributions for internal target facilities

We describe a direct method for monitoring the geometrical dimensions of a synchrotron beam at the target position for internal target installations. The method allows for the observation of the proton beam size as well as the position of the beam relative to the target. As a first demonstration of the technique, we present results obtained by means of the COSY-11 detection system installed at the cooler synchrotron COSY. The influence of the stochastic cooling on the COSY proton beam dimensions is also investigated.Comment: 7 pages, 8 figures, Submitted to Nucl. Inst. & Meth.

Near-Threshold eta Meson Production in Proton-Proton Collisions

The production of eta mesons has been measured in the proton-proton interaction close to the reaction threshold using the COSY-11 internal facility at the cooler synchrotron COSY. Total cross sections were determined for eight different excess energies in the range from 0.5 MeV to 5.4 MeV. The energy dependence of the total cross section is well described by the available phase-space volume weighted by FSI factors for the proton-proton and proton-eta pairs.Comment: 9 pages, 1 table, 5 figure

Total and Differential Cross Sections for the pp-->pp eta-prime Reaction Near Threshold

The eta-prime meson production in the reaction pp-->pp eta-prime has been studied at excess energies of Q = 26.5, 32.5 and 46.6 MeV using the internal beam facility COSY-11 at the cooler synchrotron COSY. The total cross sections as well as one angular distribution for the highest Q-value are presented. The excitation function of the near threshold data can be described by a pure s-wave phase space distribution with the inclusion of the proton-proton final state interaction and Coulomb effects. The obtained angular distribution of the eta-prime mesons is also consistent with pure s-wave production.Comment: 6 pages, 5 figures, submitted to Eur. Phys. J.

Experimental results on strangeness production in proton-proton collisions at COSY

The production of K+ and K- mesons in elementary proton-proton collision has been investigated at the Cooler Synchrotron COSY in Juelich. A high quality proton beam with low emittance and small momentum spread permitted to study the creation of these mesons very close to the kinematical threshold. The energy dependence of the total cross section is investigated using internal beam facilities providing a high accuracy particle momentum determination as well as an external non-magnetic detection setup with a large geometrical acceptance. The determination of the four-momentum vectors for all ejectiles of each registered event gives the complete kinematical information allowing to study the interaction of the outgoing particles. Results on the performed studies of the pp --> pp K+ K-, pp --> p Lambda K+ and pp --> p Sigma0 K+ reactions will be presented and their relevance to the interpretation of heavy ion collisions will be discussed.Comment: 8 pages, 6 figures, plenary talk at 6th International Conference On Strange Quarks in Matter: '2001 - A Flavorspace Odyssey' (SQM2001), Frankfurt, Germany, September 25-29, 2001, to be published in J. Phys. G: Nucl. Part. Phy

MicroRNA-101 expression is associated with JAK2V617F activity and regulates JAK2/STAT5 signaling.

Philadelphia negative myeloproliferative neopl 28 asms (MPNs) are clonal haematological diseases characterized by excessive production of mature blood cells. Exome sequencing of patient samples have showed a relatively low degree genomic complexity for these diseases1. The majority of MPN patients carry somatic mutations in the JAK2 gene, with the JAK2V617F missense mutation being the most common in poly33 cythemia vera (PV, 95%) and essential thrombocythemia (ET, 60%) 2.FP was supported by Fondazione Umberto Veronesi, and Institute Pasteur - Fondazione Cenci Bolognetti

DNA methylation regulates expression of VEGF-R2 (KDR) and VEGF-R3 (FLT4)

Abstract Background Vascular Endothelial Growth Factors (VEGFs) and their receptors (VEGF-Rs) are important regulators for angiogenesis and lymphangiogenesis. VEGFs and VEGF-Rs are not only expressed on endothelial cells but also on various subtypes of solid tumors and leukemias contributing to the growth of the malignant cells. This study was performed to examine whether VEGF-R2 (KDR) and VEGF-R3 (FLT4) are regulated by DNA methylation. Methods Real-time (RT) PCR analysis was performed to quantify KDR and FLT4 expression in some ninety leukemia/lymphoma cell lines, human umbilical vein endothelial cells (HUVECs) and dermal microvascular endothelial cells (HDMECs). Western blot analyses and flow cytometric analyses confirmed results at the protein level. After bisulfite conversion of DNA we determined the methylation status of KDR and FLT4 by DNA sequencing and by methylation specific PCR (MSP). Western blot analyses were performed to examine the effect of VEGF-C on p42/44 MAPK activation. Results Expression of KDR and FLT4 was observed in cell lines from various leukemic entities, but not in lymphoma cell lines: 16% (10/62) of the leukemia cell lines expressed KDR, 42% (27/65) were FLT4 positive. None of thirty cell lines representing six lymphoma subtypes showed more than marginal expression of KDR or FLT4. Western blot analyses confirmed KDR and FLT4 protein expression in HDMECs, HUVECs and in cell lines with high VEGF-R mRNA levels. Mature VEGF-C induced p42/44 MAPK activation in the KDR- /FLT4+ cell line OCI-AML1 verifying the model character of this cell line for VEGF-C signal transduction studies. Bisulfite sequencing and MSP revealed that GpG islands in the promoter regions of KDR and FLT4 were unmethylated in HUVECs, HDMECs and KDR + and FLT4 + cell lines, whereas methylated cell lines did not express these genes. In hypermethylated cell lines, KDR and FLT4 were re-inducible by treatment with the DNA demethylating agent 5-Aza-2'deoxycytidine, confirming epigenetic regulation of both genes. Conclusions Our data show that VEGF-Rs KDR and FLT4 are silenced by DNA methylation. However, if the promoters are unmethylated, other factors (e.g. transactivation factors) determine the extent of KDR and FLT4 expression