An Integrated, Virtualized Joint Edge and Fog Computing System with Multi-RAT Convergence

Notably, developing an innovative architectural network paradigm is essential to address the technical challenging of 5G applications' requirements in a unified platform. Forthcoming applications will provide a wide range ofnetworking, computing and storage capabilities closer to the endusers.In this context, the 5G-PPP Phase two project named "5GCORAL:A 5G Convergent Virtualized Radio Access Network Living at the Edge" aims at identifying and experimentally validating which are the key technology innovations allowing for the development of a convergent 5G multi-RAT access based on a virtualized Edge and Fog architecture being scalable, flexible and interoperable with other domains including transport, core network and distant Clouds. In 5G-CORAL, an architecture is proposed based on ETSI MEC and ETSI NFV frameworks in a unified platform. Then, a set of exemplary use cases benefiting from Edge and Fog networks in near proximity of the end-user are proposed for demonstration on top of connected car, shopping mall and high-speed train platforms.This work has been partially funded by the H2020 collaborative Europe/Taiwan research project 5G-CORAL (grant num. 761586

Evaluation of Internal Reference Genes for Quantitative Expression Analysis by Real-Time PCR in Ovine Whole Blood

The use of reference genes is commonly accepted as the most reliable approach to normalize qRT-PCR and to reduce possible errors in the quantification of gene expression. The most suitable reference genes in sheep have been identified for a restricted range of tissues, but no specific data on whole blood are available. The aim of this study was to identify a set of reference genes for normalizing qRT-PCR from ovine whole blood. We designed 11 PCR assays for commonly employed reference genes belonging to various functional classes and then determined their expression stability in whole blood samples from control and disease-stressed sheep. SDHA and YWHAZ were considered the most suitable internal controls as they were stably expressed regardless of disease status according to both geNorm and NormFinder software; furthermore, geNorm indicated SDHA/HPRT, YWHAZ/GAPDH and SDHA/YWHAZ as the best reference gene combinations in control, disease-stressed and combined sheep groups, respectively. Our study provides a validated panel of optimal control genes which may be useful for the identification of genes differentially expressed by qRT-PCR in a readily accessible tissue, with potential for discovering new physiological and disease markers and as a tool to improve production traits (e.g., by identifying expression Quantitative Trait Loci). An additional outcome of the study is a set of intron-spanning primer sequences suitable for gene expression experiments employing SYBR Green chemistry on other ovine tissues and cells

Complicaciones periodontales en las leucemias en edad pediátrica

El objetivo de este trabajo es analizar las complicaciones odonto-periodontales que se manifiestan en los pacientes pediátricos afectados por leucemias. En él se valora positivamente la intervención del odontólogo durante las fases iniciales de las leucemias, por las manifestaciones periodontales y neurológicas que se asocian a ellas, así como por las complicaciones eriodontales y alteraciones odonto-esqueléticas originadas por las terapias antiblásticas. Asimismo se hace una valoración de los tratamientos odontológicos para estos pacientes y a su educación en el ámbito higiénico-preventivo oral que beneficia el pronóstico de estos pacientes. Por último los autores enseñan los percentiles patológicos registrados en el período de observación (18 meses)