76 research outputs found
Star formation in Perseus
We present a complete survey of current star formation in the Perseus
molecular cloud, made at 850 and 450 micron with SCUBA at the JCMT. Covering 3
deg^2, this submillimetre continuum survey for protostellar activity is second
in size only to that of rho Ophiuchus (Johnstone et al. 2004). Complete above
0.4 msun (5 sigma detection in a 14'' beam), we detect a total of 91 protostars
and prestellar cores. Of these, 80% lie in clusters, representative of star
formation across the Galaxy. Two of the groups of cores are associated with the
young stellar clusters IC348 and NGC1333, and are consistent with a steady or
reduced star formation rate in the last 0.5 Myr, but not an increasing one. In
Perseus, 40--60% of cores are in small clusters (< 50 msun) and isolated
objects, much more than the 10% suggested from infrared studies. Complementing
the dust continuum, we present a C^18O map of the whole cloud at 1' resolution.
The gas and dust show filamentary structure of the dense gas on large and small
scales, with the high column density filaments breaking up into clusters of
cores. The filament mass per unit length is 5--11 msun per 0.1 pc. Given these
filament masses, there is no requirement for substantial large scale flows
along or onto the filaments in order to gather sufficient material for star
formation. We find that the probability of finding a submillimetre core is a
strongly increasing function of column density, as measured by C^18O integrated
intensity, prob(core) proportional to I^3.0. This power law relation holds down
to low column density, suggesting that there is no A_v threshold for star
formation in Perseus, unless all the low-A_v submm cores can be demonstrated to
be older protostars which have begun to lose their natal molecular cloud.Comment: 15 pages, 5 figures, bibtex file scubasf_astroph.bbl, included tex
files SSA-clusters-sorted-tidy.te
The actin-bundling protein Fascin is overexpressed in inflammatory bowel disease and may be important in tissue repair
<b>Background</b>
Fascin is associated with increased cell motility in colorectal tumours but is absent from the normal colonic epithelium. We examined the expression of fascin in inflammatory bowel disease (IBD) and its location at regions undergoing restitution and regeneration. Tissue repair is essential for disease remission and we sought to determine the effects of therapeutic modalities on fascin expression and function using an in vitro model.<p></p>
<b>Methods</b>
Immunohistochemistry was performed on colonic tissue from IBD patients to determine changes in fascin expression and distribution. A human colorectal epithelial cell line was treated with 5-aminosalicylate (a common treatment for IBD), or sodium butyrate to determine the effect on fascin expression and cell motility.<p></p>
<b>Results</b>
Fascin overexpression was observed in both ulcerative colitis and Crohn's colitis and expression correlated with disease severity. Immunoreactivity was more intense and widespread in Crohn's compared to ulcerative colitis. Interestingly, highly expressing foci were consistently observed at the edges of ulcers where flattened, motile epithelial cells are actively involved in restitution, and also in areas of mucosal regeneration.
5-aminosalicylate reduced fascin expression in colorectal epithelial cells and inhibited their motility. Conversely, sodium butyrate increased fascin expression and stimulated cell motility in the same cells.<p></p>
<b>Conclusions</b>
Our data shows that fascin is overexpressed in inflammatory bowel disease and its location is indicative of a role in tissue repair. Our in vitro studies show that different therapeutic modalities may have converse effects on fascin expression and may have significant consequences for disease remission and the clinical management of IBD
The actin-bundling protein fascin is overexpressed in colorectal adenomas and promotes motility in adenoma cells in vitro
Background:
Fascin is overexpressed in many cancers, including colorectal, but its role in the malignant transformation of benign colorectal adenomas is unclear.
Methods:
Immunohistochemical analysis of fascin expression was carried out in resected human colorectal adenoma specimens. The effects of forced overexpression of fascin on adenoma cell motility were also analysed.
Results:
We show fascin overexpression in adenomas increasing with tumour size, histological type, and degree of dysplasia and increased cell motility in adenoma cell lines following fascin transfection.
Conclusion:
These data suggest an important role for fascin in the malignant progression of colorectal tumours
The very bright SCUBA galaxy count: looking for SCUBA galaxies with the Mexican Hat Wavelet
We present the results of a search for bright high-redshift galaxies in two
large SCUBA scan-maps of Galactic regions. A Mexican Hat Wavelet technique was
used to locate point sources in these maps, which suffer high foreground
contamination as well as typical scan-map noise signatures. A catalogue of
point source objects was selected and observed again in the submillimetre
continuum, and in HCO+ (3->2) at zero redshift to rule out Galactic sources. No
extragalactic sources were found. Simulations show that the survey was
sensitive to sources with fluxes > 50 mJy, depending on the local background.
These simulations result in upper limits on the 850-micron counts of SCUBA
galaxies of 53 per square degree at 50 mJy and 2.9 per square degree at 100
mJy.Comment: Accepted by MNRA
Factors influencing patient satisfaction with dental appearance and treatments they desire to improve aesthetics
Background: We assessed factors influencing patientsâ satisfaction with their dental appearance and the
treatments they desired to improve dental aesthetics.
Methods: A cross-sectional study was performed out among 235 adult patients who visited the Hospital Universiti
Sains Malaysia dental clinic. A structured, interviewer-guided questionnaire was used to identify patient satisfaction
with their general dental appearance, cosmetic elements and desired treatments.
Results: The 235 patients consisted of 70 males (29.8%) and 165 females (70.2%), of mean age 31.5 years (SD 13.0).
Of these patients, 124 (52.8%) were not satisfied with their general dental appearance. In addition, 132 patients
(56.2%) were not happy with the color of their teeth, 76 (32.3%), regarded their teeth were poorly aligned, 62
(26.4%), as crowded and 56 (23.4%) protruded. Dissatisfaction with tooth color was significantly higher in female
than in male patients (odds ratio [OR] of 1.99 (95% confidence interval [CI] 1.13-3.50). Tooth whitening was the
treatment most desired by patients (48.1%). Results of multiple logistic regression analysis showed that patient
dissatisfaction with general dental appearance was significantly associated with female gender (OR = 2.18; 95% CI:
1.18-4.03), unhappiness with tooth color (OR = 3.05; 95% CI: 1.74-5.34) and the opinion that their teeth protruded
(OR = 2.91, 95% CI: 1.44-5.91)
Abrogation of the radiation-induced G2 checkpoint by the staurosporine derivative UCN-01 is associated with radiosensitisation in a subset of colorectal tumour cell lines
Ionising radiation is commonly used in the treatment of colorectal cancer. Tumour cells with mutant p53 undergo cell cycle arrest at G2/M after ionising radiation and evidence suggests that abrogation of this G2 arrest can lead to a premature, aberrant mitosis, thus enhancing ionising radiation-induced cell killing. The G2 checkpoint inhibitor UCN-01 was thus investigated to determine whether it would abrogate the G2 checkpoint induced by 5âGy ionising radiation in a range of colorectal tumour cell lines. Data presented show that, at doses that are alone non-toxic to the cells, UCN-01 inhibits the ionising radiation-induced G2 checkpoint in five colorectal tumour cell lines with mutant p53. The ability of UCN-01 to sensitise cells to ionising radiation-induced growth inhibition and apoptosis was also investigated and UCN-01 was found to radiosensitise two out of five cell lines. These results were confirmed by long-term colony forming efficiency studies. These results demonstrate that abrogation of the ionising radiation-induced G2 checkpoint is not necessarily associated with sensitisation to ionising radiation, however, some colorectal tumour cell lines can be radiosensitised by UCN-01. Although the mechanism of radiosensitisation is not clear, this may still be an important treatment strategy
IGFBP-rP1, a potential molecule associated with colon cancer differentiation
<p>Abstract</p> <p>Background</p> <p>In our previous studies, we have demonstrated that insulin-like growth factor binding protein-related protein1 (IGFBP-rP1) played its potential tumor suppressor role in colon cancer cells through apoptosis and senescence induction. In this study, we will further uncover the role of IGFBP-rP1 in colon cancer differentiation and a possible mechanism by revealing responsible genes.</p> <p>Results</p> <p>In normal colon epithelium, immunohistochemistry staining detected a gradient IGFBP-rP1 expression along the axis of the crypt. IGFBP-rP1 strongly expressed in the differentiated cells at the surface of the colon epithelium, while weakly expressed at the crypt base. In colon cancer tissues, the expression of IGFBP-rP1 correlated positively with the differentiation status. IGFBP-rP1 strongly expressed in low grade colorectal carcinoma and weakly expressed in high grade colorectal carcinoma. In vitro, transfection of PcDNA3.1(IGFBP-rP1) into RKO, SW620 and CW2 cells induced a more pronounced anterior-posterior polarity morphology, accompanied by upregulation with alkaline phosphatase (AKP) activity. Upregulation of carcino-embryonic antigen (CEA) was also observed in SW620 and CW2 transfectants. The addition of IGFBP-rP1 protein into the medium could mimic most but not all effects of IGFBP-rP1 cDNA transfection. Seventy-eight reproducibly differentially expressed genes were detected in PcDNA3.1(IGFBP-rP1)-RKO transfectants, using Affymetrix 133 plus 2.0 expression chip platform. Directed Acyclic Graph (DAG) of the enriched GO categories demonstrated that differential expression of the enzyme regulator activity genes together with cytoskeleton and actin binding genes were significant. IGFBP-rP1 could upreguate Transgelin (TAGLN), downregulate SRY (sex determining region Y)-box 9(campomelic dysplasia, autosomal sex-reversal) (SOX9), insulin receptor substrate 1(IRS1), cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), amphiregulin(schwannoma-derived growth factor) (AREG) and immediate early response 5-like(IER5L) in RKO, SW620 and CW2 colon cancer cells, verified by Real time Reverse Transcription Polymerase Chain Reaction (rtRT-PCR). During sodium butyrate-induced Caco2 cell differentiation, IGFBP-rP1 was upregulated and the expression showed significant correlation with the AKP activity. The downregulation of IRS1 and SOX9 were also induced by sodium butyrate.</p> <p>Conclusion</p> <p>IGFBP-rP1 was a potential key molecule associated with colon cancer differentiation. Downregulation of IRS1 and SOX9 may the possible key downstream genes involved in the process.</p
Factors Affecting Temperature Rise on the External Root Surface During Ultrasonic Retrieval of Intracanal Separated Files
A Microcomputed Tomography Scanning Study of Root Canal Space: Changes after the Ultrasonic Removal of Fractured Files
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