A novel hepatitis C virus (HCV) subtype from Somalia and its classification into HCV clade 3.

Hepatitis C virus (HCV) sequences from throughout the world have been grouped into six clades, based on recently proposed criteria. Here, the partial sequences and clade assignment are reported for three HCV isolates from chronic hepatitis C patients from Somalia, for whom conventional assays failed to identify the genotype. Phylogenetic analysis of the sequences of the core, envelope 1 and part of the non- structural 5b regions suggests that all three isolates belong to a distinct HCV genetic group, tentatively classified as subtype 3h. This novel HCV subtype shows the highest sequence similarity with HCV isolates from Indonesia. Despite the fact that these patients were infected with HCV clade 3, none of them responded to standard interferon treatment

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Numerical Simulation To Evaluate The Effect From Pre-polymerization On The Behavior Of Tubular Reactors [simulação Numérica Aplicada Para Avaliar O Efeito Da Pré-polimerização No Comportamento De Reatores Tubulares]

The present study uses a phenomenological model to simulate a continuous, two-stage polymerization process. This system is composed by a continuous stirred tank reactor (CSTR) for monomer pre-polymerization (first stage), connected to a tubular reactor (second stage) to carry out the reaction up to high conversion values. A comprehensive non-iso-thermal 2-D model (axial and radial variations) was used to predict the tubular reactor behavior. A polymer characterization model was also developed to provide estimates of the polymer average molecular weight and polydispersity. According to the results, polymerization reactions carried out in a continuous two-stage system provide a polymer with less heterogeneous properties than the one obtained in a single tubular reactor. Besides, it is possible to produce a more homogeneous polymer increasing the viscosity of the mixture fed in the tubular reactor.173250257McGreavy, C., (1994) Polymer Reactor Engineering, , 1st ed, VCH Publishers, New YorkCabral, P. A.Melo, P. A.Biscaia Jr., E. C.Lima, E. L. & Pinto, J. C. - Polym. Eng. Sci., 43, 6, p.1163 (2003);Tien, N.-K.Flaschel, E. & Renken, A. - Polymer Reaction Engineering - Influence of Reaction Engineering on Polymer Properties, Hanser Publishers (1983);Chen, C.C., (1994) Polym. Plast. Technol. Eng, 33 (1), p. 55Lynn, S., Huff, J.E., (1971) AIChe J, 17 (2), p. 475Husain, A., Hamielec, A.E., (1976) AIChe Symp. Series, 72 (160), p. 112Nogueira, A.L., Lona, L.M.F., Machado, R.A.F., (2004) J. Appl. Pol. Sci, 91, p. 871Soroush, M., Kravaris, C., (1993) AIChe J, 39, p. 1920Marten, F.L., Hamielec, A.E., (1982) J. Appl. Pol. Sci, 27, p. 489Stevens, C.J., Mathematical Modeling of Bulk and Solution Free Radical Polymerization in Tubular Reactors (1988), Ph.D. Thesis, University of Winscosin, Madison, EUAHui, A., Hamielec, A.E., (1968) J. Pol. Sci, 25, p. 167Hamer, J.W., Ray, W.H., (1986) Chem. Eng. Sci, 41 (12), p. 3083Holman, J.P., (1963) Heat Transfer, , Mc Graw Hill, New YorkChen, C.C., Nauman, E.B., (1989) Chem. Eng. Sci, 44 (1), p. 179Cutter, L. A. & Drexler, T. D. - Comp. Appl. Pol. Sci., p.13 (1982);Hui, A., Hamielec, A.E., (1972) J. Appl. Pol. Sci, 16, p. 749Husain, A., Hamielec, A.E., (1978) J. Appl. Pol. Sci, 22, p. 1207Kricheldorf, H.R., (1992) Handbook of Polymer Synthesis, , Marcel Dekker, New YorkMcLaughlin, H. S.Mallikarjun, R. & Nauman, E. B. - AIChe J., 32, 3, p.419 (1986);Wyman, C.E., Carter, L.F., (1976) AIChe Symp. Ser, 72 (160), p. 1Powell, F.E., Brooks, B.W., (1995) Chem. Eng. Sci, 50 (5), p. 837Soliman, M. A.Aljarboa, T. & Alahmad, M. - Polym. Eng. Sci., 34, p.1464 (1994);Tossun, G., (1992) AIChe J, 38, p. 42

Oral Exposure to Silver/Gold Nanoparticles: Status of RatLipid Profile, Serum Metabolites and Tissue Morphology

Study investigated the effect of oral administration of gold-silver nanoparticleson rat biochemical parameters and tissue morphology. Wistar rats weighingapproximately 180±7 g were randomly assigned into four groups. Animals in thecontrol group received distilled water once daily for 30 days while, those in thetreatment groups were administered 10, 50 and 100 mg kgG1 b.wt. gold-silvernanoparticles. The rats were sacrificed under slight anesthesia, 24 h after the lasttreatments. Blood and vital organs including the heart, kidney and liver werecollected and prepared for biochemical and histopathological determinations.Exposure to Ag/Au nanoparticles altered the rat serum lipid profile; lowering theHDL-C while raising the atherogenic index. Exposure of Ag/Au nanoparticles inrats caused significant alteration to the levels of serum albumin, total protein,bilirubin, urea and creatinine. The activities of alanine transaminase, aspartatetransaminase and alkaline phosphatase in rat serum and tissues were alsosignificantly altered by Ag/Au nanoparticles exposure. The histopathologicalexamination revealed inflammation and cellular degeneration caused by exposureto the Ag/Au nanoparticles. We show evidence that Ag/Au nanoparticles elevatedatherogenic index, as well as caused biochemical and morphological alterations,reminiscent of cellular injur

Associations between COVID-19 testing status, non-communicable diseases and HIV status among residents of sub-Saharan Africa during the first wave of the pandemic

Background This study determined if non-communicable disease status, HIV status, COVID-19 status and co-habiting were associated with COVID-19 test status in sub-Saharan Africa. Methods Data of 5945 respondents age 18-years-old and above from 31 countries in sub-Saharan Africa collected through an online survey conducted between June and December 2020, were extracted. The dependent variable was COVID-19 status (testing positive for COVID-19 and having symptoms of COVID-19 but not getting tested). The independent variables were non-communicable disease status (hypertension, diabetes, cancer, heart conditions, respiratory conditions, depression), HIV positive status, COVID-19 status (knowing a close friend who tested positive for COVID-19 and someone who died from COVID-19) and co-habiting (yes/no). Two binary logistic regression models developed to determine associations between the dependent and independent variables were adjusted for age, sex, employment, sub region and educational status. Results Having a close friend who tested positive for COVID-19 (AOR:6.747), knowing someone who died from COVID-19 infection (AOR:1.732), and living with other people (AOR:1.512) were significantly associated with higher odds of testing positive for COVID-19 infection, while living with HIV was associated with significantly lower odds of testing positive for COVID-19 infection (AOR:0.284). Also, respondents with respiratory conditions (AOR:2.487), self-reported depression (AOR:1.901), those who had a close friend who tested positive for COVID-19 infection (AOR:2.562) and who knew someone who died from COVID-19 infection (AOR:1.811) had significantly higher odds of having symptoms of COVID-19 infection but not getting tested. Conclusion Non-communicable diseases seem not to increase the risk for COVID-19 positive test while cohabiting seems to reduce this risk. The likelihood that those who know someone who tested positive to or who died from COVID-19 not getting tested when symptomatic suggests there is poor contact tracing in the region. People with respiratory conditions and depression need support to get tested for COVID-19

Factors associated with COVID-19 pandemic induced post-traumatic stress symptoms among adults living with and without HIV in Nigeria: a cross-sectional study

Background Nigeria is a country with high risk for traumatic incidences, now aggravated by the COVID-19 pandemic. This study aimed to identify differences in COVID-19 related post-traumatic stress symptoms (PTSS) among people living and not living with HIV; to assess whether PTSS were associated with COVID-19 pandemic-related anger, loneliness, social isolation, and social support; and to determine the association between PTSS and use of COVID-19 prevention strategies. Methods The data of the 3761 respondents for this analysis was extracted from a cross-sectional online survey that collected information about mental health and wellness from a convenience sample of adults, 18 years and above, in Nigeria from July to December 2020. Information was collected on the study's dependent variable (PTSS), independent variables (self-reported COVID-19, HIV status, use of COVID-19 prevention strategies, perception of social isolation, access to emotional support, feelings of anger and loneliness), and potential confounder (age, sex at birth, employment status). A binary logistic regression model tested the associations between independent and dependent variables. Results Nearly half (47.5%) of the respondents had PTSS. People who had symptoms but were not tested (AOR = 2.20), felt socially isolated (AOR = 1.16), angry (AOR = 2.64), or lonely (AOR = 2.19) had significantly greater odds of reporting PTSS (p < 0.001). People living with HIV (AOR = 0.39), those who wore masks (AOR = 0.62) and those who had emotional support (AOR = 0.63), had lower odds of reporting PTSS (p < .05). Conclusion The present study identified some multifaceted relationships between post-traumatic stress, HIV status, facemask use, anger, loneliness, social isolation, and access to emotional support during this protracted COVID-19 pandemic. These findings have implications for the future health of those affected, particularly for individuals living in Nigeria. Public health education should be incorporated in programs targeting prevention and prompt diagnosis and treatment for post-traumatic stress disorder at the community level

Factors Associated with Financial Security, Food Security and Quality of Daily Lives of Residents in Nigeria during the First Wave of the COVID-19 Pandemic

An online survey was conducted to identify factors associated with financial insecurity, food insecurity and poor quality of daily lives of adults in Nigeria during the first wave of the COVID-19 pandemic. The associations between the outcome (experience of financial loss, changes in food intake and impact of the pandemic on daily lives) and the explanatory (age, sex, education level, anxiety, depression, HIV status) variables were determined using logistic regression analysis. Of the 4439 respondents, 2487 (56.0%) were financially insecure, 907 (20.4%) decreased food intake and 4029 (90.8%) had their daily life negatively impacted. Males (AOR:0.84), people who felt depressed (AOR:0.62) and people living with HIV -PLHIV- (AOR:0.70) had significantly lower odds of financial insecurity. Older respondents (AOR:1.01) had significantly higher odds of financial insecurity. Those depressed (AOR:0.62) and PLHIV (AOR:0.55) had significantly lower odds of reporting decreased food intake. Respondents who felt anxious (AOR:0.07), depressed (AOR: 0.48) and who were PLHIV (AOR:0.68) had significantly lower odds of reporting a negative impact of the pandemic on their daily lives. We concluded the study findings may reflect a complex relationship between financial insecurity, food insecurity, poor quality of life, mental health, and socioeconomic status of adults living in Nigeria during the COVID-19 pandemic

Differences in COVID-19 Preventive Behavior and Food Insecurity by HIV Status in Nigeria

The aim of the study was to assess if there were significant differences in the adoption of COVID-19 risk preventive behaviors and experience of food insecurity by people living with and without HIV in Nigeria. This was a cross-sectional study that recruited a convenience sample of 4471 (20.5% HIV positive) adults in Nigeria. Binary logistic regression analysis was conducted to test the associations between the explanatory variable (HIV positive and non-positive status) and the outcome variables-COVID-19 related behavior changes (physical distancing, isolation/quarantine, working remotely) and food insecurity (hungry but did not eat, cut the size of meals/skip meals) controlling for age, sex at birth, COVID-19 status, and medical status of respondents. Significantly fewer people living with HIV (PLWH) reported a positive COVID-19 test result; and had lower odds of practicing COVID-19 risk preventive behaviors. In comparison with those living without HIV, PLWH had higher odds of cutting meal sizes as a food security measure (AOR: 3.18; 95% CI 2.60-3.88) and lower odds of being hungry and not eating (AOR: 0.24; 95% CI 0.20-0.30). In conclusion, associations between HIV status, COVID-19 preventive behaviors and food security are highly complex and warrant further in-depth to unravel the incongruities identified