309 research outputs found
Small herbivores and abiotic heterogeneity promote trait variation of a saltmarsh plant in local communities
Intraspecific trait variation (ITV) enables plants to respond to global changes. However, causes for ITV, especially from biotic components such as herbivory, are not well understood. We explored whether small vertebrate herbivores (hares and geese) impact ITV of a dominant clonal plant (Elytrigia atherica) in local communities. Moreover, we looked at the relative importance of their direct (e.g., selective grazing) and indirect effects (altering genotypic richness/diversity and abiotic environment) on ITV. We used exclosures at two successional stages in a Dutch saltmarsh, where grazing pressure at the early successional stage was ca. 1.5 times higher than that of the intermediate successional stage. We measured key functional traits of E. atherica including height, aboveground biomass, flowering (flower or not), specific leaf area, and leaf dry matter content in local communities (1 m × 1 m plots) inside and outside the exclosures. We determined genotypic richness and diversity of each plant using molecular markers. We further measured abiotic variations in topography and clay thickness (a proxy for soil total nitrogen). Structural equation models revealed that small herbivores significantly promoted ITV in height and flowering at the early successional stage, while they marginally promoted ITV in height at the intermediate successional stage. Moreover, the direct effects of herbivores played a major role in promoting ITV. Small herbivores decreased genotypic diversity at the intermediate successional stage, but genotypic richness and diversity did not impact ITV. Small herbivores did not alter topographic variation and variation in clay thickness, but these variations increased ITV in all traits at the early successional stage. Small herbivores may not only impact trait means in plants as studies have shown but also their ITV
Corrosion inhibition of 7150 Al alloy in the presence of some amino acids – molecular dynamics simulation, quantum chemical, and electrochemical studies
Trajectory Data Collection with Local Differential Privacy
Trajectory data collection is a common task with many applications in our
daily lives. Analyzing trajectory data enables service providers to enhance
their services, which ultimately benefits users. However, directly collecting
trajectory data may give rise to privacy-related issues that cannot be ignored.
Local differential privacy (LDP), as the de facto privacy protection standard
in a decentralized setting, enables users to perturb their trajectories locally
and provides a provable privacy guarantee. Existing approaches to private
trajectory data collection in a local setting typically use relaxed versions of
LDP, which cannot provide a strict privacy guarantee, or require some external
knowledge that is impractical to obtain and update in a timely manner. To
tackle these problems, we propose a novel trajectory perturbation mechanism
that relies solely on an underlying location set and satisfies pure
-LDP to provide a stringent privacy guarantee. In the proposed
mechanism, each point's adjacent direction information in the trajectory is
used in its perturbation process. Such information serves as an effective clue
to connect neighboring points and can be used to restrict the possible region
of a perturbed point in order to enhance utility. To the best of our knowledge,
our study is the first to use direction information for trajectory perturbation
under LDP. Furthermore, based on this mechanism, we present an anchor-based
method that adaptively restricts the region of each perturbed trajectory,
thereby significantly boosting performance without violating the privacy
constraint. Extensive experiments on both real-world and synthetic datasets
demonstrate the effectiveness of the proposed mechanisms.Comment: Accepted by VLDB 202
Immune checkpoints expression patterns in early-stage triple-negative breast cancer predict prognosis and remodel the tumor immune microenvironment
BackgroundCurrently, targeting immune checkpoint molecules holds great promise for triple-negative breast cancer (TNBC). However, the expression landscape of immune checkpoint genes (ICGs) in TNBC remains largely unknown.MethodHerein, we systematically investigated the ICGs expression patterns in 422 TNBC samples. We evaluated the ICGs molecular typing based on the ICGs expression profile and explored the associations between ICGs molecular subtypes and tumor immune characteristics, clinical significance, and response to immune checkpoint inhibitors (ICIs).ResultsTwo ICGs clusters and two ICGs-related gene clusters were determined, which were involved in different survival outcomes, biological roles and infiltration levels of immune cells. We established a quantification system ICGs riskscore (named IRS) to assess the ICGs expression patterns for individuals. TNBC patients with lower IRS were characterized by increased immune cell infiltration, favorable clinical outcomes and high sensitivity to ICIs therapy. We also developed a nomogram model combining clinicopathological variables to predict overall survival in TNBC. Genomic feature analysis revealed that high IRS group presented an increased tumor mutation burden compared with the low IRS group.ConclusionCollectively, dissecting the ICGs expression patterns not only provides a new insight into TNBC subtypes but also deepens the understanding of ICGs in the tumor immune microenvironment
Non-Markovian epidemic spreading on temporal networks
Many empirical studies have revealed that the occurrences of contacts
associated with human activities are non-Markovian temporal processes with a
heavy tailed inter-event time distribution. Besides, there has been increasing
empirical evidence that the infection and recovery rates are time-dependent.
However, we lack a comprehensive framework to analyze and understand
non-Markovian contact and spreading processes on temporal networks. In this
paper, we propose a general formalism to study non-Markovian dynamics on
non-Markovian temporal networks. We find that, under certain conditions,
non-Markovian dynamics on temporal networks are equivalent to Markovian
dynamics on static networks. Interestingly, this result is independent of the
underlying network topology
Population dynamics of bearded croaker Johnius dussumieri (Cuvier, 1830) from Pakistani waters
In this study the length frequency data of 2510 bearded croaker Johnius dussumieri (Cuvier, 1830), collected from the coast of Pakistan during 2015, were analysed. Total length of the specimens (male and female combined) varied from 4 to 25 cm with dominant individuals ranged between 12 and 15 cm whereas the body weight varied between 3 and 155 g. The length frequency data were analysed for the estimation of population dynamics and the power coefficient b of length weight relationship was estimated as 2.83. Other measurements were as follows: asymptotic length, L∞ = 26.25 cm; growth coefficient, K = 1.00 year–1; total mortality, Z = 2.43 year–1; and natural mortality, M = 1.82 year–1. The fishing mortality (F) and exploitation ratio (E) were 0.61 year–1 and 0.251 respectively. The Biological Reference Points (BRPs) with Gulland method for this fishery (Fopt) was estimated 1.82 year–1 which is higher than current fish mortality. Therefore the present study shows that the J. dussumieri fishery is safe in Pakistan
Long-term cross-scale comparison of grazing and mowing on plant diversity and community composition in a salt-marsh system
Land abandonment has been increasing in recent decades in Europe, usually accompanied by biodiversity decline. Whether livestock grazing and mowing can safeguard biodiversity across spatial scales in the long term is unclear. Using a 48-year experiment in a salt marsh, we compared land abandonment (without grazing and mowing) and seven management regimes including cattle grazing, early season mowing, late season mowing, both early and late season mowing, and grazing plus each of the mowing regimes on plant diversity at the local and larger scales (i.e. aggregated local communities). Also, we compared their effects on community composition (both in identities and abundances) in time and space. Under land abandonment, plant diversity declined in the local communities and this decline became more apparent at the larger scale, particularly for graminoids and halophytes. All management regimes, except for late season mowing, maintained plant diversity at these scales. Local plant communities under all treatments underwent different successional trajectories, in the end, diverged from their initial state except for that under grazing (a cyclic succession). Year-to-year changes in local community composition remained at a similar level over time under land abandonment and grazing plus early season mowing while it changed under other treatments. Vegetation homogenized at the larger scale over time under land abandonment while vegetation remained heterogeneous under all management regimes. Synthesis. Our experiment suggests that late season mowing may not be sustainable to conserve plant diversity in salt marshes. Other management regimes can maintain plant diversity across spatial scales and vegetation heterogeneity at the larger scale in the long term, but local community composition may change over time.Fil: Chen, Qingqing. University of Groningen; Países BajosFil: Bakker, Jan P.. University of Groningen; Países BajosFil: Alberti, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Bakker, Elisabeth S.. Netherlands Institute of Ecology; Países Bajos. University of Agriculture Wageningen; Países BajosFil: Smit, Christian. University of Groningen; Países BajosFil: Olff, Han. University of Groningen; Países Bajo
Legionella pneumophila strain 130b evades macrophage cell death independent of the effector SidF in the absence of flagellin
International audienceThe human pathogen Legionella pneumophila must evade host cell death signaling to enable replication in lung macrophages and to cause disease. After bacterial growth, however, L. pneumophila is thought to induce apoptosis during egress from macrophages. The bacterial effector protein, SidF, has been shown to control host cell survival and death by inhibiting pro-apoptotic BNIP3 and BCL-RAMBO signaling. Using live-cell imaging to follow the L. pneumophila-macrophage interaction, we now demonstrate that L. pneumophila evades host cell apoptosis independent of SidF. In the absence of SidF, L. pneumophila was able to replicate, cause loss of mitochondria membrane potential, kill macrophages, and establish infections in lungs of mice. Consistent with this, deletion of BNIP3 and BCL-RAMBO did not affect intracellular L. pneumophila replication, macrophage death rates, and in vivo bacterial virulence. Abrogating mitochondrial cell death by genetic deletion of the effectors of intrinsic apoptosis, BAX, and BAK, or the regulator of mitochondrial permeability transition pore formation, cyclophilin-D, did not affect bacterial growth or the initial killing of macrophages. Loss of BAX and BAK only marginally limited the ability of L. pneumophila to efficiently kill all macrophages over extended periods. L. pneumophila induced killing of macrophages was delayed in the absence of capsase-11 mediated pyroptosis. Together, our data demonstrate that L. pneumophila evades host cell death responses independently of SidF during replication and can induce pyroptosis to kill macrophages in a timely manner
Effect of different doses of dexmedetomidine on lung function and tissue cell apoptosis in a rat model of hyperoxic acute lung injury
Purpose: To study the effect of different doses of dexmedetomidine on lung function and lung tissue cell apoptosis in a rat model of hyperoxic acute lung injury.
Methods: Five groups of healthy male Sprague-Dawley rats were used: normal rats, untreated hyperoxic rats, and hyperoxic rats given 3 different doses of dexmedetomidine, with 20 rats in each group. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were determined usingenzyme-linked immunosorbent assay (ELISA). Parietal paraffin cuts were taken from the right upper lobe for measurement of apoptosis using in situ terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and the apoptosis index was calculated.
Results: At 24 and 48 h, the levels of IL-6 and TNF-α in the hyperoxia model group were significantly higher than those in the normal control group, and their levels in the middle- and high-dose groups were markedly lowered, relative to untreated hyperoxia rats (p < 0.05). Apoptosis index in the hyperoxia model rats significantly increased, relative to normal rats (p < 0.05). The apoptosis index in the mediumand high-dose groups decreased significantly (p < 0.05).
Conclusion: Dexmedetomidine inhibits inflammatory responses caused by high concentration of oxygen inhalation, minimizes lung injury, improves lung function and inhibits lung apoptosis.
Keywords: Dexmedetomidine, Hyperoxia, Acute lung injury, Lung function, Apoptosi
Small herbivores slow down species loss up to 22 years but only at early successional stage
The long-term influence of persistent small herbivores on successional plant community configuration is rarely studied. We used a herbivore exclusion experiment along the successional gradient in a salt-marsh system, to investigate the effects of hares and geese, and hares alone, on plant diversity at five successional stages (the earliest, two early, the intermediate and the late successional stages) in the short and long term, i.e. 7 and 22 years, respectively. Plant diversity declined over time at all successional stages except for the earliest one. Small herbivores slowed down species decline, but only at one early successional stage. Small herbivores slowed down species decline via decreasing dominance of preferred grass Festuca rubra in the short term, and less preferred Elytrigia atherica in the long term. The effects of hares and geese were more pronounced than hares alone, indicating an important additive role of geese, especially in the long term. Synthesis. Small herbivores can have a strong and long-lasting impact on plant diversity, but it highly depends on the abundance of small herbivores, which in turn depends on the quality and abundance of forage plants. A diverse herbivore community may have more positive effects on regulating plant communities
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