2,3,4,6-Tetra-O-acetyl-1-O-(4-methoxy­cinnamo­yl)-β-d-glucopyran­ose

Mol­ecules of the title compound, C24H28O12, are linked by inter­molecular C—H⋯O hydrogen bonds. Bond lengths and angles are normal

Benzyl 3-(10-oxo-9,10-dihydrophenanthren-9-ylidene)dithiocarbazate

In the title compound, C22H16N2OS2, the phenanthrene ring is nearly perpendicular to the phenyl ring, making a dihedral angle of 87.2 (2)°. Intra­molecular N—H⋯O inter­actions are present. In the crystal structure, the mol­ecules are linked through inter­molecular C—H⋯O inter­actions. The crystal structure is also stabilized by C—H⋯π inter­actions and weak π–π contacts [centroid-centroid distance = 3.36 (6) Å]

Cationic Porphyrins with Large Side Arm Substituents as Resonance Light Scattering Ratiometric Probes for Specific Recognition of Nucleic Acid G-quadruplexes

Specific G-quadruplex-probing is crucial for both biological sciences and biosensing applications. Most reported probes are focused on fluorescent or colorimetric recognition of G-quadruplexes. Herein, for the first time, we reported a new specific G-quadruplex-probing technique—resonance light scattering (RLS)-based ratiometric recognition. To achieve the RLS probing of G-quadruplexes in the important physiological pH range of 7.4-6.0, four water soluble cationic porphyrin derivatives, including an unreported octa-cationic porphyrin, with large side arm substituents were synthesized and developed as RLS probes. These RLS probes were demonstrated to work well for ratiometric recognition of G-quadruplexes with high specificity against single- and double-stranded DNAs, including long double-stranded ones. The working mechanism was speculated to be based on the RLS signal changes caused by porphyrin protonation that was promoted by the end-stacking of porphyrins on Gquadruplexes. This work adds an important member in G-quadruplex probe family, thus providing a useful tool for studies on G-quadruplex-related events concerning G-quadruplex formation, destruction and changes in size, shape and aggregation. As a proofof-concept example of applications, the RLS probes were demonstrated to work well for label-free and sequence-specific sensing of microRNA. This work also provides a simple and useful way for the preparation of cationic porphyrins with high charges

2-[Bis(3,5-dimethyl­phen­yl)­phosphor­yl]­propan-2-ol hemihydrate

In the organic mol­ecule of the title compound, C19H25O2P·0.5H2O, the benzene rings are oriented at a dihedral angle of 54.04 (3)°. Intra­molecular C—H⋯O hydrogen bonds result in the formation of two five-membered planar rings, which are oriented with respect to the adjacent benzene rings at dihedral angles of 2.66 (3) and 2.79 (3)°. In the crystal structure, inter­molecular O—H⋯O hydrogen bonds link the mol­ecules. The water oxygen atom lies on a twofold rotation axis

Excessive and asymmetrical removal of heterozygous sites by maxSH biases downstream population genetic inference: Implications for hybridization between two primroses

Techniques of reduced-representation sequencing (RRS) have revolutionized ecological and evolutionary genomics studies. Precise establishment of orthologs is a critical challenge for RRS, especially when a reference genome is absent. The proportion of shared heterozygous sites across samples is an alternative criterion for filtering paralogs. In the prevailing pipeline for variant calling of RRS data – PYRAD/IPYRAD, maxSH is an often overlooked parameter with implications to detecting and filtering paralogs according to shared heterozygosity. Using empirical genotyping by sequencing data of two primroses (Primula alpicola Stapf and Primula florindae Ward) and their putative hybrids, and extra data sets of Californian golden cup oaks, we explore the impact of maxSH on filtering paralogs and further downstream analyses. Our study sheds light on the simultaneous validity and risk of filtering paralogs using maxSH, and its significant effects on downstream analyses of outlier detection, population assignment, and demographic modeling, emphasizing the importance of attention to detail during bioinformatic processes. The mutual confirmation between results of population assignment and demographic modeling in this study suggested maxSH = 0.10 has a potentially excessive and asymmetrical effect on the removal of truly shared heterozygous sites as paralogs. These results indicate that hybridization origin hypotheses of putative hybrids represented by results with maxSH = 0.25 and 0.50 are more credible. In conclusion, we revealed the critical hazard of paralogs filtration according to sharing heterozygosity at first, so that we propose to use specific protocols, rather than maxSH, to filter potential paralogs for closely related lineages.info:eu-repo/semantics/acceptedVersio

(S)-(–)-Methyl 2-(p-tolyl­sulfon­yloxy)­propanoate

In the title compound, C11H14O5S, there is an intra­molecular C—H⋯O hydrogen bond, for which the C—C—S—O torsion angle involving the acceptor and donor atoms is 2.4 (4)°. The dihedral angle between the benzene ring and the methoxy­carbonyl plane is 52.7 (4)°. In the crystal structure, mol­ecules are linked via inter­molecular C—H⋯O hydrogen bonds, forming a mol­ecular chain along the b axis

3,7-Dihydr­oxy-3,7-diphenyl-2H,6H-pyrrolo[3,4-f]isoindole-1,5(3H,7H)-dione methanol disolvate

The asymmetric unit of the title compound, C22H16N2O4·2CH4O, contains one half-mol­ecule and a methanol solvent mol­ecule. The aromatic ring is oriented at a dihedral angle of 82.91 (3)° with respect to the planar indole ring systems. In the crystal structure, inter­molecular O—H⋯O and N—H⋯O hydrogen bonds link the mol­ecules into chains along the b axis