How Emotional Attachment Effects Intention: The Case of Continuous Knowledge Sharing Intention in Virtual Community

Most existing researches explore continuous knowledge - sharing from the perspective of the rational cognition. This paper argues that the continuous intention and behaviors should be influenced by long-term emotional factors, and it can be explored from the perspective of emotional attachment to the influential factors of continuous knowledge sharing willingness of virtual communities. Based on the Expectation Confirmation Theory, this paper introduced the attachment and constructed a model of virtual community users\u27 continuous knowledge sharing intention. Structural equation modeling method is used to analyze the data and verify the model. The results show that attachment not only has a direct positive effect on the long-term knowledge sharing intention of virtual community users, but also mediated the relationship between satisfaction, perceived usefulness and the intention of continuous knowledge sharing

A Meta-Analysis

Background p16INK4a is a tumor suppressor protein which is induced in cells upon the interaction of high-risk HPV E7 with the retinoblastoma protein by a positive feedback loop, but cannot exert its suppressing effect. Previous reports suggested that p16INK4a immunostaining allows precise identification of even small CIN or cervical cancer lesions in biopsies. The prognostic value of overexpressed p16INK4a in cervical cancer has been evaluated for several years while the results remain controversial. We performed a systematic review and meta-analysis of studies assessing the clinical and prognostic significance of overexpression of p16INK4a in cervical cancer. Methods Identification and review of publications assessing clinical or prognostic significance of p16INK4a overexpression in cervical cancer until March 1, 2014. A meta-analysis was performed to clarify the association between p16INK4a overexpression and clinical outcomes. Results A total of 15 publications met the criteria and comprised 1633 cases. Analysis of these data showed that p16INK4a overexpression was not significantly associated with tumor TNM staging (I+II vs. III+IV) (OR = 0.75, 95% confidence interval [CI]: 0.35–1.63, P = 0.47), the tumor grade (G1+ G2 vs. G3) (OR = 0.78, 95% CI: 0.39–1.57, P = 0.49), the tumor size (<4 vs. ≥4 cm) (OR = 1.10, 95% CI: 0.45–2.69, P = 0.83), or vascular invasion (OR = 1.20, 95% CI: 0.69–2.08, P = 0.52). However, in the identified studies, overexpression of p16INK4a was highly correlated with no lymph node metastasis (OR = 0.51, 95% CI: 0.28–0.95, P = 0.04), increased overall survival (relative risk [RR]: 0.42, 95% CI: 0.24–0.72, P = 0.002) and increased disease free survival (RR: 0.60, 95% CI: 0.44–0.82, P = 0.001)

Preventive effects of atorvastatin on atherosclerosis in experimental rabbits are associated with reduction in cholesterol level and VCAM-1 expression

In this study, blood samples of rabbits were collected to measure plasma total cholesterol, total triglyceride, and LDL-cholesterol. After 16 weeks of high cholesterol diet with or without atorvastatin treatment, the rabbits were sacrificed and morphological changes in tissues were examined with hematoxylin and eosin (HE) staining, and the expression of vascular-cell adhesion molecule 1 (VCAM-1) was determined by immunostaining and reverse transcription-polymerase chain reaction (RT-PCR). We demonstrate that atorvastatin significantly reduced plasma levels of total cholesterol (41.7 %) and LDLcholesterol (34.6 %). Neither the hyper cholesterol diet nor atorvastatin treatment had any significant impact on body weight and plasma triglycerides (TG). Atorvastatin significantly restored the intima with widening of 40.9 % and even down-regulated the ratio of intima/media by 55.5 %. The inhibitory effects of atorvastatin on the expression of VCAM-1 showed a decrease of up to 46.9 % (P < 0.01). The diseased rabbits showed a 63.2 % increase in VCAM-1 mRNA expression (P < 0.01), which was reversed by nearly 60 % by treatment with atorvastatinColegio de Farmacéuticos de la Provincia de Buenos Aire

Coping strategies following the diagnosis of a fetal anomaly: A scoping review

IntroductionMany women experience severe emotional distress (such as grief, depression, and anxiety) following a diagnosis of fetal anomaly. The ability to cope with stressful events and regulate emotions across diverse situations may play a primary role in psychological wellbeing. This study aims to present coping strategies after disclosing a fetal anomaly to pregnant women.MethodsThis is a scoping review based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) extension for scoping reviews (PRISMA-ScR). Electronic databases, including Web of Science (WOS, BCI, KJD, MEDLINE, RSCI, SCIELO), CINAHL, and EBSCO PsycARTICLES, were used to search for primary studies from the inception of each database to 2021. The keywords were determined by existing literature and included: “fetal anomaly,” “fetal abnormality,” “fetal anomaly,” “fetal abnormality” AND “cope,” “coping,” “deal,” “manage,” “adapt*,” “emotion* regulate*,” with the use of Boolean operators AND/OR. A total of 16 articles were reviewed, followed by advancing scoping review methodology of Arksey and O'Malley's framework.ResultsIn this review, we identified 52 coping strategies using five questionnaires in seven quantitative studies and one mixed-method study. The relationship between coping strategies and mental distress was explored. However, the results were inconsistent and incomparable. We synthesized four coping categories from qualitative studies and presented them in an intersection.ConclusionThis scoping review identified the coping strategies of women with a diagnosis of a fetal anomaly during pregnancy. The relationship between coping strategies and mental distress was uncertain and needs more exploration. We considered an appropriate measurement should be necessary for the research of coping in women diagnosed with fetal anomaly pregnancy

An observational study on the effect of seasonal variation on peritoneal dialysis patients

Background: Seasonal variation has an impact on plants, wild animals, and also human beings. Data have shown seasonal variation has a significant impact on patients’ fluid status, biochemistry results, and outcomes in hemodialysis populations. The relevant data on peritoneal dialysis is scant.Methods: This was a cross sectional study. All patients followed up in our center had a peritoneal equilibration test and PD adequacy test every 6 months. All the peritoneal equilibration test and PD adequacy test data were collected during December 2019 to November 2020. The monthly delivery information of the whole center was collected from 2015 to 2019.Results: There were 366 patients and 604 sets of peritoneal equilibration test and PD adequacy test results in the study. Plasma albumin and phosphate levels were higher in summer. The monthly average outdoor temperature was positively correlated with plasma albumin. There was no seasonal difference in peritoneal dialysis ultrafiltration or urine volume. The percentage of low glucose concentration (1.5%) usage was higher in summer and lower in winter.Conclusion: Plasma albumin and phosphate levels were higher in summer in PD patients. Weaker glucose peritoneal dialysis dialysate was more widely used in summer. Understanding the seasonal variation of peritoneal dialysis is helpful in individualized treatment

Immune and oxidative stress disorder in ovulation-dysfunction women revealed by single-cell transcriptome

IntroductionOvulation dysfunction is now a widespread cause of infertility around the world. Although the impact of immune cells in human reproduction has been widely investigated, systematic understanding of the changes of the immune atlas under female ovulation remain less understood.MethodsHere, we generated single cell transcriptomic profiles of 80,689 PBMCs in three representative statuses of ovulation dysfunction, i.e., polycystic ovary syndrome (PCOS), primary ovarian insufficiency (POI) and menopause (MENO), and identified totally 7 major cell types and 25 subsets of cells.ResultsOur study revealed distinct cluster distributions of immune cells among individuals of ovulation disorders and health. In patients with ovulation dysfunction, we observed a significant reduction in populations of naïve CD8 T cells and effector memory CD4 T cells, whereas circulating NK cells and regulatory NK cells increased.DiscussionOur results highlight the significant contribution of cDC-mediated signaling pathways to the overall inflammatory response within ovulation disorders. Furthermore, our data demonstrated a significant upregulation of oxidative stress in patients with ovulation disorder. Overall, our study gave a deeper insight into the mechanism of PCOS, POI, and menopause, which may contribute to the better diagnosis and treatments of these ovulatory disorder

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

City Centrality, Migrants and Green Inovation Efficiency: Evidence from 106 Cities in the Yangtze River Economic Belt of China

Based on the panel data of 106 cities in the Yangtze River Economic Belt of China from 2007 to 2016, this paper explores the impact of city centrality on the green innovation efficiency and proves the mediation effect of migrants by using spatial econometric model. The results show that there are more and more innovation contacts between cities, and the innovation network is becoming more and more dense. The core cities of the downstream innovation network are mainly Yangzhou, Zhenjiang, Wuxi, Changzhou, Suzhou and Hangzhou; the core cities in the midstream are mainly Wuhan, Changsha and Yichun; the core cities in the upstream are Chengdu and Bazhong. There is an inverted U-shaped relationship between city centrality and green innovation efficiency. In addition, the influence curve of city centrality on the green innovation efficiency of surrounding cities is also inverted U-shaped. Cities with high city centrality attract a large number of migrants that come from cities with lower centrality to improve the green innovation efficiency, but the green innovation efficiency of cities with low city centrality will decline due to lack of talents

Effects of microwave time on quality of grass carp fillets processed through microwave combined with hot-air drying

In this study, the quality changes of grass carp fillets dried by microwave-hot-air combined drying under different microwave time were investigated. The salted fillets were dried at 385 W microwave with different time (0, 2, 4, 6, 8, and 10 min), followed by 65℃ hot air to the end. The quality of fillets was evaluated by drying time, color, hardness, rehydration ratio, and taste characteristics (ATP-related compounds, free amino acids, E-tongue taste profile, EUC, and TAV). Results showed that grass carp fillets dried by microwave-hot-air combined drying had better qualities compared with single hot-air drying. Besides, microwave time had obvious effects on the quality changes of grass carp fillets, and 6 min was considered as the optimal drying time

Application of Uncertain AHP Method in Analyzing Travel Time Belief Reliability in Transportation Network

Because predictions of transportation system reliability can provide useful information for intelligent transportation systems (ITS), evaluation of them might be viewed as a beneficial activity for reducing traffic congestion. This evaluation procedure could include some alternatives and criteria in a discrete decision space. To handle this evaluation process in an uncertain environment, a novel uncertain multi-criteria decision-making (MCDM) method is put forward in this paper. Considering the validity of uncertainty theory as a measure of epistemic uncertainty, we first introduce it into analytic hierarchy process (AHP) and provide the whole calculation procedure of the approach. The proposed approach is employed to evaluate regional travel time belief reliability in a case study. Additionally, a comparison is performed between the results of uncertain AHP and other MCDM methods to examine the efficiency of this method. These analyses show that uncertainty theory is particularly suited to be employed combination with the AHP method