Profiling analysis of long non-coding RNAs in early postnatal mouse hearts

Mammalian cardiomyocytes undergo a critical hyperplastic-to-hypertrophic growth transition at early postnatal age, which is important in establishing normal physiological function of postnatal hearts. In the current study, we intended to explore the role of long non-coding (lnc) RNAs in this transitional stage. We analyzed lncRNA expression profiles in mouse hearts at postnatal day (P) 1, P7 and P28 via microarray. We identified 1,146 differentially expressed lncRNAs with more than 2.0-fold change when compared the expression profiles of P1 to P7, P1 to P28, and P7 to P28. The neighboring genes of these differentially expressed lncRNAs were mainly involved in DNA replication-associated biological processes. We were particularly interested in one novel cardiac-enriched lncRNA, ENSMUST00000117266, whose expression was dramatically down-regulated from P1 to P28 and was also sensitive to hypoxia, paraquat, and myocardial infarction. Knockdown ENSMUST00000117266 led to a significant increase of neonatal mouse cardiomyocytes in G0/G1 phase and reduction in G2/M phase, suggesting that ENSMUST00000117266 is involved in regulating cardiomyocyte proliferative activity and is likely associated with hyperplastic-to-hypertrophic growth transition. In conclusion, our data have identified a large group of lncRNAs presented in the early postnatal mouse heart. Some of these lncRNAs may have important functions in cardiac hyperplastic-to-hypertrophic growth transition

Spatiotemporal dynamic of subtropical forest carbon storage and its resistance and resilience to drought in China

Subtropical forests are rich in vegetation and have high photosynthetic capacity. China is an important area for the distribution of subtropical forests, evergreen broadleaf forests (EBFs) and evergreen needleleaf forests (ENFs) are two typical vegetation types in subtropical China. Forest carbon storage is an important indicator for measuring the basic characteristics of forest ecosystems and is of great significance for maintaining the global carbon balance. Drought can affect forest activity and may even lead to forest death and the stability characteristics of different forest ecosystems varied after drought events. Therefore, this study used meteorological data to simulate the standardized precipitation evapotranspiration index (SPEI) and the Biome-BGC model to simulate two types of forest carbon storage to quantify the resistance and resilience of EBF and ENF to drought in the subtropical region of China. The results show that: 1) from 1952 to 2019, the interannual drought in subtropical China showed an increasing trend, with five extreme droughts recorded, of which 2011 was the most severe one; 2) the simulated average carbon storage of the EBF and ENF during 1985-2019 were 130.58 t·hm-2 and 78.49 t·hm-2, respectively. The regions with higher carbon storage of EBF were mainly concentrated in central and southeastern subtropics, where those of ENF mainly distributed in the western subtropic; 3) The median of resistance of EBF was three times higher than that of ENF, indicating the EBF have stronger resistance to extreme drought than ENF. Moreover, the resilience of two typical forest to 2011 extreme drought and the continuous drought events during 2009 - 2011 were similar. The results provided a scientific basis for the response of subtropical forests to drought, and indicating that improve stand quality or expand the plantation of EBF may enhance the resistance to drought in subtropical China, which provided certain reference for forest protection and management under the increasing frequency of drought events in the future

Thermal stability of biochar and its effects on cadmium sorption capacity

In this study, the thermal stability of a wood shaving biochar (WS, 650 °C), a chicken litter biochar (CL, 550 °C) and an activated carbon (AC, 1100 °C) were evaluated by combustion at 375 °C for 24 h to remove the labile non-carbonized organic matter. Results showed that WS and CL biochars were not thermally stable and can lose most of the organic C during combustion. The combusted WS and CL biochars retained considerable amounts of negative charge and displayed higher sorption for Cd (from 5.46 to 68.9 mg/g for WS and from 48.5 to 60.9 mg/g for CL). The AC retained 76.5% of its original C and became more negatively chargely after combustion, but its sorption for Cd slightly decreased (from 18.5 to 14.9 mg/g). This study indicated that after potential burning in wildfires (200 - 500 °C), biochars could have higher sorption capacity for metals by remaining minerals

Pyrogenic carbon in Australian soils

Pyrogenic carbon (PyC), the combustion residues of fossil fuel and biomass, is a versatile soil fraction active in biogeochemical processes. In this study, the chemo-thermal oxidation method (CTO-375) was applied to investigate the content and distribution of PyC in 30 Australian agricultural, pastoral, bushland and parkland soil with various soil types. Soils were sampled incrementally to 50 cm in 6 locations and at another 7 locations at 0–10 cm. Results showed that PyC in Australian soils typically ranged from 0.27–5.62 mg/g, with three Dermosol soils ranging within 2.58–5.62 mg/g. Soil PyC contributed 2.0–11% (N = 29) to the total organic carbon (TOC), with one Ferrosol as high as 26%. PyC was concentrated either in the top (0–10 cm) or bottom (30–50 cm) soil layers, with the highest PyC:TOC ratio in the bottom (30–50 cm) soil horizon in all soils. Principal component analysis - multiple linear regression (PCA-MLR) suggested the silt-associated organic C factor accounted for 38.5% of the variation in PyC. Our findings suggest that PyC is an important fraction of the TOC (2.0–11%, N = 18) and chemically recalcitrant organic C (ROC) obtained by chemical C fractionation method accounts for a significant proportion of soil TOC (47.3–84.9%, N = 18). This is the first study comparing these two methods, and it indicates both CTO-375 and C speciation methods can determine a fraction of recalcitrant organic C. However, estimated chemically recalcitrant organic carbon pool (ROC) was approximately an order of magnitude greater than that of thermally stable organic carbon (PyC)

Effects of acidic and neutral biochars on properties and cadmium retention of soils

In this study, an acidic biochar and a neutral biochar were applied at 5 wt% into two soils for an 11-month incubation experiment. One Ferrosol soil (Ba) was slightly acidic with low organic matter and the other Dermosol soil (Mt) was slightly alkaline with high organic matter. The acidic (pH = 3.25) wood shaving (WS) biochar had no marked impact on nutrient levels, cation exchange capacity (CEC), pH and acid neutralisation capacity (ANC) of either soil. By contrast, the neutral (pH = 7.00) chicken litter (CL) biochar significantly increased major soluble nutrients, pH, ANC of soil Ba. In terms of C storage, 87.9% and 69.5% WS biochar-C can be sequestrated as TOC by soil Ba and Mt, respectively, whereas only 24.0% of CL biochar-C stored in soil Ba and negligible amount in Mt as TOC. Biochars did not have significant effects on soil sorption capacity and sorption reversibility except that CL biochar increased sorption of soil Ba by around 25.4% and decreased desorption by around 50.0%. Overall, the studied acidic C rich WS biochar held little agricultural or remedial values but was favourable for C sequestration. The neutral mineral rich CL biochar may provide short-term agricultural benefit and certain sorption capacities of lower sorption capacity soils, but may be unlikely to result in heightened C sequestration in soils. This is the first study comprehensively examining functions of acidic and neutral biochars for their benefits as a soil amendment and suggests the importance of pre-testing biochars for target purposes prior to their large scale production

Land Use Function Transition and Associated Ecosystem Service Value Effects Based on Production–Living–Ecological Space: A Case Study in the Three Gorges Reservoir Area

The transition of land use function and its effects on ecosystem services is a key issue in eco-environmental protection and is the basis of territorial space governance and optimization. Previous studies have typically selected land use types to evaluate ecosystem service value (ESV) and have overlooked comprehensive characteristics of ecosystem services and the mutual feedback relationship between human social systems and the ecosystem. Taking the Three Gorges Reservoir Area, Hubei section (TGRA-HS) as a case study, we used a transition matrix, the revised ESV method, and an ecological contribution rate model to explore land use function transition (LUFT) and its effects on the change in ESV based on the production–living–ecological space (PLES) classification system. The results show that: (1) The transition of land use function based on PLES was the mapping of the evolution of the human–nature relationship in the spatial pattern, which reflected the evolution of the spatial pattern caused by human interference with the continuous development of society; (2) The evolution of PLES showed the characteristics of a reduction in production space (P-space), and an expansion in living space (L-space) and ecological space (E-space). The distribution pattern of PLES from 1990 to 2020 was basically the same, and the characteristics of structural transform reflected the characteristics of project construction in different phases; (3) The E-space contributed the most to the total ESV, and it has risen by CNY 13.06 × 108. The transition of land use function caused by human construction projects impacts the spatiotemporal change in the regional ESV; (4) The change in ESV induced by LUFT revealed the whole dynamic process of the positive and negative effects of human construction projects on ecosystem services, and the two effects offset each other to keep the ESV relatively stable. The transition of E-space to P-space had the greatest impact on the reduction in ESV, whose contribution rate was 82.76%. The dynamic changes in land use function and ESV corresponding to the different stages of the Three Gorges Project’s (TGP) construction reveals the important driving effect of human activities on ecosystem services. It reminds us that humans should not forget to protect the eco-environment when obtaining services from the ecosystem

Land Use Function Transition and Associated Ecosystem Service Value Effects Based on Production–Living–Ecological Space: A Case Study in the Three Gorges Reservoir Area

The transition of land use function and its effects on ecosystem services is a key issue in eco-environmental protection and is the basis of territorial space governance and optimization. Previous studies have typically selected land use types to evaluate ecosystem service value (ESV) and have overlooked comprehensive characteristics of ecosystem services and the mutual feedback relationship between human social systems and the ecosystem. Taking the Three Gorges Reservoir Area, Hubei section (TGRA-HS) as a case study, we used a transition matrix, the revised ESV method, and an ecological contribution rate model to explore land use function transition (LUFT) and its effects on the change in ESV based on the production–living–ecological space (PLES) classification system. The results show that: (1) The transition of land use function based on PLES was the mapping of the evolution of the human–nature relationship in the spatial pattern, which reflected the evolution of the spatial pattern caused by human interference with the continuous development of society; (2) The evolution of PLES showed the characteristics of a reduction in production space (P-space), and an expansion in living space (L-space) and ecological space (E-space). The distribution pattern of PLES from 1990 to 2020 was basically the same, and the characteristics of structural transform reflected the characteristics of project construction in different phases; (3) The E-space contributed the most to the total ESV, and it has risen by CNY 13.06 × 108. The transition of land use function caused by human construction projects impacts the spatiotemporal change in the regional ESV; (4) The change in ESV induced by LUFT revealed the whole dynamic process of the positive and negative effects of human construction projects on ecosystem services, and the two effects offset each other to keep the ESV relatively stable. The transition of E-space to P-space had the greatest impact on the reduction in ESV, whose contribution rate was 82.76%. The dynamic changes in land use function and ESV corresponding to the different stages of the Three Gorges Project’s (TGP) construction reveals the important driving effect of human activities on ecosystem services. It reminds us that humans should not forget to protect the eco-environment when obtaining services from the ecosystem

GSDMD-mediated pyroptosis promotes cardiac remodeling in pressure overload

Background Gasdermin D (GSDMD) forms membrane pores to execute pyroptosis. But the mechanism of how cardiomyocyte pyroptosis induces cardiac remodeling in pressure overload remains unclear. We investigated the role of GSDMD-mediated pyroptosis in the pathogenesis of cardiac remodeling in pressure overload. Methods Wild-type (WT) and cardiomyocyte-specific GSDMD-deficient (GSDMD-CKO) mice were subjected to transverse aortic constriction (TAC) to induce pressure overload. Four weeks after surgery, left ventricular structure and function were evaluated by echocardiographic, invasive hemodynamic and histological analysis. Pertinent signaling pathways related to pyroptosis, hypertrophy and fibrosis were investigated by histochemistry, RT-PCR and western blotting. The serum levels of GSDMD and IL-18 collected from healthy volunteers or hypertensive patients were measured by ELISA. Results We found TAC induced cardiomyocyte pyroptosis and release of pro-inflammatory cytokines IL-18. The serum GSDMD level was significantly higher in hypertensive patients than in healthy volunteers, and induced more dramatic release of mature IL-18. GSDMD deletion remarkably mitigated TAC-induced cardiomyocyte pyroptosis. Furthermore, GSDMD deficiency in cardiomyocytes significantly reduced myocardial hypertrophy and fibrosis. The deterioration of cardiac remodeling by GSDMD-mediated pyroptosis was associated with activating JNK and p38 signaling pathways, but not ERK or Akt signaling pathway. Conclusion In conclusion, our results demonstrate that GSDMD serves as a key executioner of pyroptosis in cardiac remodeling induced by pressure overload. GSDMD-mediated pyroptosis activates JNK and p38 signaling pathways, and this may provide a new therapeutic target for cardiac remodeling induced by pressure overload

Influence of Brönsted Acid Sites on Activated Carbon-Based Catalyst for Acetylene Dimerization

Activated carbon (AC) has been widely used as a support material with both tunable acidity and abundant functional groups for solid acid catalysts in various chemical processes such as acetylene dimerization. A facile, mild acid modification method that directly activates AC to generate rich defects and oxygen functional group surface structures with Brönsted acid sites and an enhanced conductivity is presented here. Impressively, the catalyst with optimized Brönsted acid sites and an enhanced dispersion of active components exhibited a superior acetylene dimerization catalytic activity. Moreover, theoretical calculations indicated that an increase in hydrogen concentration could inhibit the formation of coke. This research offered a feasible potential way to devise and construct a carbon-based solid acid catalyst with an excellent catalytic performance

M6A-mediated upregulation of HOXC10 promotes human hepatocellular carcinoma development through PTEN/AKT/mTOR signaling pathway

Abstract Human Hox genes (Homeobox) play a crucial role in embryonic development and cancer. The HOXC10 gene, a member of the HOX family, has been reported abnormally expressed in several cancers. However, the association between HOXC10 and hepatocellular carcinoma (HCC) remains to be elucidated. In the present study, tissue microarray cohort data showed that high levels of HOXC10 expression predicted a poor survival in HCC patients. Meanwhile, HOXC10 was significantly upregulated in the Huh7 cell line compared with the well differentiated cell line HepG2 and human normal liver cells. Functionally, silencing HOXC10 in Huh7 cells inhibited cell proliferation, increased apoptosis, and inhibited invasion and migration of HCC cells. HOXC10 overexpression in HepG2 cells increased cell proliferation, decreased apoptosis, and increased invasion and migration of HCC cells. In the HepG2 xenograft models, HOXC10 increased the tumor volume and weight compared with control. Mechanistically, the m6A modification of HOXC10 by METTL3 enhanced its expression by enhancing its mRNA stability. Both the in vitro and in vivo results showed that overexpressed HOXC10 activated the PTEN/AKT/mTOR pathway. In summary, the findings highlight the importance of HOXC10 in the regulation of HCC progression. HOXC10 is potentially a future therapeutic target for HCC treatment