218 research outputs found
Molecular cytogenetic aberrations in patients with multiple myeloma studied by interphase fluorescence in situ hybridization
Background: Multiple myeloma (MM) is an incurable hematological disorder characterized by the accumulation of malignant plasma cells within the bone marrow (BM). The clinical heterogeneity of MM is dictated by the cytogenetic aberrations present in the clonal plasma cells (PCs). Cytogenetic studies in MM are hampered by the hypoproliferative nature of plasma cells in MM. Therefore, fluorescence in situ hybridization (FISH) analysis combined with magnetic-activated cell sorting (MACS) is an attractive alternative for evaluation of numerical and structural chromosomal changes in MM. Methods: Interphase FISH studies with three different specific probes for the regions containing 13q14.3 (D13S319), 14q32 (IGHC/IGHV) and 1q12(CEP1 ) were performed in 48 MM patients. Interphase FISH studies with LSI IGH/CCND1, LSI IGH/FGFR3, and LSI IGH/MAF probes were used to detect t(11;14)(q13;q32), t(4;14)(p16;q32), and t(14;16)(q32;q23) in patients with 14q32 rearrangement. Results: Molecular cytogenetic aberrations were found in 40 (83.3%) of the 48 MM patients. 13 patients (27.1%) simultaneously had 13q deletion/monosomy 13 [del(13q14)], illegitimate IGH rearrangement and chromosome 1 abnormality. Del(13q14) was detected in 21 cases (43.7%), and illegitimate IGH rearrangements in 29 (60.4%) including 6 with t(11;14) and 5 with t(4;14). None of 9 patients with illegitimate IGH rearrangements and without t(11;14) or t(4;14) we detected had t(14;16) (q32;q23). 24 of the 48 MM patients (50%) had chromosome 1 abnormalities. Among 21 patients with del(13q14), 15 patients had Amp1q12;16 had IgH rearrangements. Whereas, among 27 cases without del(13q14), 8 had Amp1q12; 13 had IgH rearrangements. There was a strong association between del(13q14) and Amp1q12(c2 = 8.26, Ρ < 0.01), and between del(13q14) and IgH rearrangement(c2 = 3.88, p < 0.05). Conclusion: 13q deletion/monosomy 13, IGH rearrangement and chromosome 1 abnormality are frequent in MM. They are not randomly distributed, but strongly interconnected. Interphase FISH technique combined with MACS using CD138-specific antibody is a highly sensitive technique at detecting molecular cytogenetic aberrations in MM.ΠΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠ΅: ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½Π°Ρ ΠΌΠΈΠ΅Π»ΠΎΠΌΠ° (MM) β Π½Π΅ΠΈΠ·Π»Π΅ΡΠΈΠΌΠΎΠ΅ Π³Π΅ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠ΅, Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΈΡΡΡΡΠ΅Π΅ΡΡ
Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ΠΌ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΠΊΠΎΡΡΠ½ΠΎΠΌ ΠΌΠΎΠ·Π³Π΅ (ΠM). ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠ°Ρ Π³Π΅ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΡ MM ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΡΡΡ
ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ Π°Π±Π΅ΡΡΠ°ΡΠΈΡΠΌΠΈ, ΠΏΡΠΈΡΡΡΡΡΠ²ΡΡΡΠΈΠΌΠΈ Π² ΠΊΠ»ΠΎΠ½Π΅ ΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠ»Π΅ΡΠΎΠΊ (ΠΠ). Π¦ΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ
MM ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½Ρ Π³ΠΈΠΏΠΎΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΠΌΠΈ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΡΠΌΠΈ ΠΠ. Π ΡΠ²ΡΠ·ΠΈ Ρ ΡΡΠΈΠΌ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½Π°Ρ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ in situ (FISH)
Π² ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ ΡΠΎΡΡΠΈΡΠΎΠ²ΠΊΠΎΠΉ ΠΊΠ»Π΅ΡΠΎΠΊ, Π°ΠΊΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΌΠ°Π³Π½ΠΈΡΠ½ΡΠΌΠΈ ΠΏΠΎΠ»ΡΠΌΠΈ (MACS) ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΡΡΡ Π΄ΠΎΡΡΠΎΠΉΠ½ΠΎΠΉ Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²ΠΎΠΉ
ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠΎΡΠ΅ΡΠ½ΡΡ
ΠΈ ΡΡΡΡΠΊΡΡΡΠ½ΡΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ ΠΏΡΠΈ MM. ΠΠ΅ΡΠΎΠ΄Ρ: ΠΈΠ½ΡΠ΅ΡΡΠ°Π·Π½ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ
FISH Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΡΠ΅Ρ
ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π·ΠΎΠ½Π΄ΠΎΠ² Π΄Π»Ρ ΡΡΠ°ΡΡΠΊΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
13q14.3 (D13S319), 14q32
(IGHC/IGHV) ΠΈ 1q12(CEP1), ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Ρ 48 Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ MM. ΠΠ½ΡΠ΅ΡΡΠ°Π·Π½ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ FISH Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ
Π·ΠΎΠ½Π΄ΠΎΠ² LSI IGH/CCND1, LSI IGH/FGFR3 ΠΈ LSI IGH/MAF ΠΏΡΠΈΠΌΠ΅Π½ΡΠ»ΠΈ Π΄Π»Ρ Π΄Π΅ΡΠ΅ΠΊΡΠΈΠΈ t(11;14)(q13;q32), t(4;14)(p16;q32), ΠΈ
t(14;16)(q32;q23) Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠΎΠΉ 14q32. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π°Π±Π΅ΡΡΠ°ΡΠΈΠΈ Π²ΡΡΠ²Π»ΡΠ»ΠΈ Ρ
40 (83,3%) ΠΈΠ· 48 Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ MM. Π£ 13 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² (27,1%) ΠΎΠ΄Π½ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ 13q Π΄Π΅Π»Π΅ΡΠΈΡ/ΠΌΠΎΠ½ΠΎΡΠΎΠΌΠΈΡ 13 [del(13q14)],
Π°Π½ΠΎΠΌΠ°Π»ΡΠ½Π°Ρ ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠ° IGH ΠΈ Π°Π½ΠΎΠΌΠ°Π»ΠΈΡ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 1. Del(13q14) Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π² 21 ΡΠ»ΡΡΠ°Π΅ (43,7%), Π° Π°Π½ΠΎΠΌΠ°Π»ΡΠ½ΡΠ΅
ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠΈ IGH β Π² 29 (60,4%), Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Ρ 6 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ t(11;14) ΠΈ 5 Ρ t(4;14). ΠΠΈ Ρ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΈΠ· 9 Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ Π°Π½ΠΎΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ
ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠ°ΠΌΠΈ IGH ΠΈ Π±Π΅Π· t(11;14) ΠΈΠ»ΠΈ t(4;14) Π½Π΅ Π²ΡΡΠ²Π»ΡΠ»ΠΈ ΡΡΠ°Π½ΡΠ»ΠΎΠΊΠ°ΡΠΈΡ t(14;16) (q32;q23). Π£ 24 ΠΈΠ· 48 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ MM
(50%) ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π°Π½ΠΎΠΌΠ°Π»ΠΈΠΈ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 1. Π Π³ΡΡΠΏΠΏΠ΅ ΠΈΠ· 21 Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ del(13q14) Π² 15 ΡΠ»ΡΡΠ°ΡΡ
ΠΈΠΌΠ΅Π»ΠΈΡΡ ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠΈ IgH
Amp1q12;16. Π ΡΠΎ ΠΆΠ΅ Π²ΡΠ΅ΠΌΡ ΠΈΠ· 27 ΡΠ»ΡΡΠ°Π΅Π² Π±Π΅Π· del(13q14) Ρ 8 ΡΠΎΠ΄Π΅ΡΠΆΠ°Π»ΠΈΡΡ Amp1q12; Π² 13 ΡΠ»ΡΡΠ°ΡΡ
ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠΈ
IgH. ΠΡΡΠ²Π»Π΅Π½Π° Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Ρ ΠΌΠ΅ΠΆΠ΄Ρ del(13q14) ΠΈ Amp1q12(Ο2
= 8,26, p < 0,01) ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ del(13q14) ΠΈ ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠ°ΠΌΠΈ IgH
(Ο2 = 3,88, p < 0,05). ΠΡΠ²ΠΎΠ΄Ρ: 13q Π΄Π΅Π»Π΅ΡΠΈΡ/ΠΌΠΎΠ½ΠΎΡΠΎΠΌΠΈΡ 13, ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΡ IGH ΠΈ Π°Π½ΠΎΠΌΠ°Π»ΠΈΡ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 1 ΡΠ°ΡΡΠΎ ΠΎΡΠΌΠ΅ΡΠ°ΡΡ
ΠΏΡΠΈ MM, ΠΏΡΠΈΡΠ΅ΠΌ ΠΈΡ
ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π½Π΅ ΡΠ»ΡΡΠ°ΠΉΠ½ΠΎ ΠΈ ΡΠ΅ΡΠ½ΠΎ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Π°Π½ΠΎ. ΠΠ½ΡΠ΅ΡΡΠ°Π·Π½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· FISH Π² ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ
MACS Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ CD138-ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π» ΡΠ²Π»ΡΠ΅ΡΡΡ Π²ΡΡΠΎΠΊΠΎΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π΄Π΅ΡΠ΅ΠΊΡΠΈΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π°Π±Π΅ΡΡΠ°ΡΠΈΠΉ ΠΏΡΠΈ MM
Learning Shape Segmentation Using Constrained Spectral Clustering and Probabilistic Label Transfer
International audienceWe propose a spectral learning approach to shape segmentation. The method is composed of a constrained spectral clustering algorithm that is used to supervise the segmentation of a shape from a training data set, followed by a probabilistic label transfer algorithm that is used to match two shapes and to transfer cluster labels from a training-shape to a test-shape. The novelty resides both in the use of the Laplacian embedding to propagate must-link and cannot-link constraints, and in the segmentation algorithm which is based on a learn, align, transfer, and classify paradigm. We compare the results obtained with our method with other constrained spectral clustering methods and we assess its performance based on ground-truth data
Measurements of the observed cross sections for exclusive light hadrons containing at , 3.650 and 3.6648 GeV
By analyzing the data sets of 17.3, 6.5 and 1.0 pb taken,
respectively, at , 3.650 and 3.6648 GeV with the BES-II
detector at the BEPC collider, we measure the observed cross sections for
, , ,
and at the three energy
points. Based on these cross sections we set the upper limits on the observed
cross sections and the branching fractions for decay into these
final states at 90% C.L..Comment: 7 pages, 2 figure
Partial wave analysis of J/\psi \to \gamma \phi \phi
Using events collected in the BESII detector, the
radiative decay is
studied. The invariant mass distribution exhibits a near-threshold
enhancement that peaks around 2.24 GeV/.
A partial wave analysis shows that the structure is dominated by a
state () with a mass of
GeV/ and a width of GeV/. The
product branching fraction is: .Comment: 11 pages, 4 figures. corrected proof for journa
Direct Measurements of Absolute Branching Fractions for D0 and D+ Inclusive Semimuonic Decays
By analyzing about 33 data sample collected at and around 3.773
GeV with the BES-II detector at the BEPC collider, we directly measure the
branching fractions for the neutral and charged inclusive semimuonic decays
to be and , and determine the ratio of the two branching
fractions to be
Measurements of the observed cross sections for exclusive light hadron production in e^+e^- annihilation at \sqrt{s}= 3.773 and 3.650 GeV
By analyzing the data sets of 17.3 pb taken at GeV
and 6.5 pb taken at GeV with the BESII detector at the
BEPC collider, we have measured the observed cross sections for 12 exclusive
light hadron final states produced in annihilation at the two energy
points. We have also set the upper limits on the observed cross sections and
the branching fractions for decay to these final states at 90%
C.L.Comment: 8 pages, 5 figur
Search for the Rare Decays J/Psi --> Ds- e+ nu_e, J/Psi --> D- e+ nu_e, and J/Psi --> D0bar e+ e-
We report on a search for the decays J/Psi --> Ds- e+ nu_e + c.c., J/Psi -->
D- e+ nu_e + c.c., and J/Psi --> D0bar e+ e- + c.c. in a sample of 5.8 * 10^7
J/Psi events collected with the BESII detector at the BEPC. No excess of signal
above background is observed, and 90% confidence level upper limits on the
branching fractions are set: B(J/Psi --> Ds- e+ nu_e + c.c.)<4.8*10^-5, B(J/Psi
--> D- e+ nu_e + c.c.) D0bar e+ e- + c.c.)<1.1*10^-5Comment: 10 pages, 4 figure
Measurements of psi(2S) decays to octet baryon-antibaryon pairs
With a sample of 14 million psi(2S) events collected by the BESII detector at
the Beijing Electron Positron Collider (BEPC), the decay channels psi(2S)->p
p-bar, Lambda Lambda-bar, Sigma0 Sigma0-bar, Xi Xi-bar are measured, and their
branching ratios are determined to be (3.36+-0.09+-0.24)*10E-4,
(3.39+-0.20+-0.32)*10E-4, (2.35+-0.36+-0.32)*10E-4, (3.03+-0.40+-0.32)*10E-4,
respectively. In the decay psi(2S)->p p-bar, the angular distribution parameter
alpha is determined to be 0.82+-0.17+-0.04.Comment: 8 pages, 8 figure
A study of charged kappa in
Based on events collected by BESII, the decay
is studied. In the invariant mass
spectrum recoiling against the charged , the charged
particle is found as a low mass enhancement. If a Breit-Wigner function of
constant width is used to parameterize the kappa, its pole locates at MeV/. Also in this channel,
the decay is observed for the first time.
Its branching ratio is .Comment: 14 pages, 4 figure
Direct Measurements of the Branching Fractions for and and Determinations of the Form Factors and
The absolute branching fractions for the decays and
are determined using singly
tagged sample from the data collected around 3.773 GeV with the
BES-II detector at the BEPC. In the system recoiling against the singly tagged
meson, events for and events for decays are observed. Those yield
the absolute branching fractions to be and . The
vector form factors are determined to be
and . The ratio of the two form
factors is measured to be .Comment: 6 pages, 5 figure
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