Czech Version of the Spiritual Well-Being Scale: Evaluation and Psychometric Properties

Spirituality and spiritual well-being are connected with many areas of human life. Thus, especially in secular countries, there is a need for reliable validated instruments for measuring spirituality. The Spiritual Well-Being Scale is among the world’s most often used tools; therefore, the aim of this study was its psychometrical evaluation in the secular environment of the Czech Republic on a nationally representative sample (n = 1797, mean age: 45.9 ± 17.67; 48.6% men). A non-parametric comparison of different sociodemographic groups showed a higher disposition for experiencing spirituality among women, older people, and divorced persons. Based on confirmatory factor analysis, negatively worded items were excluded using a polychoric correlation matrix. The new version of the scale consisting of 11 items had good internal consistency (Cronbach’s α = 0.85; McDonald’s ωt = 0.91). The two-factor model of this shortened version, with factors corresponding to the Religious and the Existential subscales of the Spiritual Well-Being Scale, shows a satisfactory fit with the data, where the loadings of all items ranged from medium to high. Thus, this study offered a new version of the tool, convenient for measuring spiritual well-being in secular conditions

The Spiritual Well-Being Scale:Psychometric Evaluation of the Shortened Version in Czech Adolescents

The aim of this study was to psychometrically evaluate the shortened version of the Spiritual Well-Being Scale (SWBS) in Czech adolescents. A nationally representative sample of 4217 adolescents participated in the 2014 Health Behaviour in School-aged Children survey. The internal consistency of the SWBS was assessed using Cronbach's alpha (alpha) and Mean Inter-Item Correlation (MIIC) values. The factor structure was evaluated using principal component analyses. After adjustment, our new seven-item version of the scale supports a two-factorial model of the SWBS with satisfactory internal consistency (alpha = 0.814, MIIC = 0.379). This version of the SWBS is suitable for measuring spiritual well-being in a secularising environment

Virulence Characteristics and Genetic Affinities of Multiple Drug Resistant Uropathogenic Escherichia coli from a Semi Urban Locality in India

Extraintestinal pathogenic Escherichia coli (ExPEC) are of significant health concern. The emergence of drug resistant E. coli with high virulence potential is alarming. Lack of sufficient data on transmission dynamics, virulence spectrum and antimicrobial resistance of certain pathogens such as the uropathogenic E. coli (UPEC) from countries with high infection burden, such as India, hinders the infection control and management efforts. In this study, we extensively genotyped and phenotyped a collection of 150 UPEC obtained from patients belonging to a semi-urban, industrialized setting near Pune, India. The isolates representing different clinical categories were analyzed in comparison with 50 commensal E. coli isolates from India as well as 50 ExPEC strains from Germany. Virulent strains were identified based on hemolysis, haemagglutination, cell surface hydrophobicity, serum bactericidal activity as well as with the help of O serotyping. We generated antimicrobial resistance profiles for all the clinical isolates and carried out phylogenetic analysis based on repetitive extragenic palindromic (rep)-PCR. E. coli from urinary tract infection cases expressed higher percentages of type I (45%) and P fimbriae (40%) when compared to fecal isolates (25% and 8% respectively). Hemolytic group comprised of 60% of UPEC and only 2% of E. coli from feces. Additionally, we found that serum resistance and cell surface hydrophobicity were not significantly (p = 0.16/p = 0.51) associated with UPEC from clinical cases. Moreover, clinical isolates exhibited highest resistance against amoxicillin (67.3%) and least against nitrofurantoin (57.3%). We also observed that 31.3% of UPEC were extended-spectrum beta-lactamase (ESBL) producers belonging to serotype O25, of which four were also positive for O25b subgroup that is linked to B2-O25b-ST131-CTX-M-15 virulent/multiresistant type. Furthermore, isolates from India and Germany (as well as global sources) were found to be genetically distinct with no evidence to espouse expansion of E. coli from India to the west or vice-versa