Dark homogeneous streak dermoscopic pattern correlating with specific KIT mutations in melanoma

Mutations driving melanoma growth have diagnostic, prognostic, and therapeutic implications. Traditional classification systems do not correlate optimally with underlying melanoma growth-promoting mutations. Our objective was to determine whether unique dermoscopic growth patterns directly correlate with driving mutations. OBSERVATIONS: We evaluated common driving mutations in 4 different dermoscopic patterns (rhomboidal, negative pigmented network, polygonal, and dark homogeneous streaks) of primary cutaneous melanomas; 3 melanomas per pattern were tested. Three of the 4 patterns lacked common mutations in BRAF, NRAS, KIT, GNAQ, and HRAS. One pattern, the dark homogeneous streaks pattern, had unique KIT mutations in the second catalytic domain of KIT in exon 17 for all 3 samples tested. Two tumors with the dark homogeneous streaks pattern turned out to be different primary melanomas from the same patient and had different sequence mutations but had an impact on the same KIT domain. CONCLUSIONS AND RELEVANCE: While future study is required, these results have multiple implications. (1) The underlying melanoma-driving mutations may give rise to specific dermoscopic growth patterns, (2) BRAF/NRAS mutations in early melanomas may not be as common as previously thought, and (3) patients may be predisposed to developing specific driving mutations giving rise to melanomas or nevi of similar growth patterns

Grafted tomato conducted to more than one branch: ecophisiological variables and growth potential

El injerto en cultivo de tomate, vigoriza a las plantas, pudiendo conducirlas a más de una rama. Con el objetivo de evaluar variables ecofisiológicas en tomate injertado,en un invernadero en La Plata (Buenos Aires), se condujo un ensayo de Ichiban F1 injertado sobre Maxifort F1 (Seminis®)(T1), conducido a una (1R),(T2) dos (2R) y (T3)4 (4R) ramas y (T) Ichiban sin injertar a una rama.Facultad de Ciencias Agrarias y Forestale

Grafted tomato conducted to more than one branch: ecophisiological variables and growth potential

El injerto en cultivo de tomate, vigoriza a las plantas, pudiendo conducirlas a más de una rama. Con el objetivo de evaluar variables ecofisiológicas en tomate injertado,en un invernadero en La Plata (Buenos Aires), se condujo un ensayo de Ichiban F1 injertado sobre Maxifort F1 (Seminis®)(T1), conducido a una (1R),(T2) dos (2R) y (T3)4 (4R) ramas y (T) Ichiban sin injertar a una rama.Facultad de Ciencias Agrarias y Forestale

Grafted tomato conducted to more than one branch: ecophisiological variables and growth potential

El injerto en cultivo de tomate, vigoriza a las plantas, pudiendo conducirlas a más de una rama. Con el objetivo de evaluar variables ecofisiológicas en tomate injertado,en un invernadero en La Plata (Buenos Aires), se condujo un ensayo de Ichiban F1 injertado sobre Maxifort F1 (Seminis®)(T1), conducido a una (1R),(T2) dos (2R) y (T3)4 (4R) ramas y (T) Ichiban sin injertar a una rama.Facultad de Ciencias Agrarias y Forestale

Ecofisiología de los cultivos protegidos: trabajos de experimentación hacia una horticultura sustentable : Proyecto 11 A/269

En el marco del Proyecto de Investigación se realizaron ensayos con el objetivo de responder diversas inquietudes del sector productivo del Cordón Hortícola Platense (CHP). A continuación se enumeran los trabajos realizados y presentados en Jornadas y Congresos de la especialidad, como así también resultados de diversas Tesis de Grado realizadas por alumnos de la FCAyF, UNLP.Facultad de Ciencias Agrarias y Forestale

Ecofisiología de los cultivos protegidos: trabajos de experimentación hacia una horticultura sustentable : Proyecto 11 A/269

En el marco del Proyecto de Investigación se realizaron ensayos con el objetivo de responder diversas inquietudes del sector productivo del Cordón Hortícola Platense (CHP). A continuación se enumeran los trabajos realizados y presentados en Jornadas y Congresos de la especialidad, como así también resultados de diversas Tesis de Grado realizadas por alumnos de la FCAyF, UNLP.Facultad de Ciencias Agrarias y Forestale

Corales de aguas frías en los cañones de Cap de Creus y Lacaze- Duthiers (noroeste del Mediterráneo): distribución batimétrica, estructura de tallas y morfología

XVII Simposio Ibérico de Estudios de Biología Marina (SIEBM), 11-14 September 2012, Donostia-San SebastiánPeer reviewe

Validity and Reliability of Dermoscopic Criteria Used to Differentiate Nevi From Melanoma: A Web-Based International Dermoscopy Society Study.

IMPORTANCE: The comparative diagnostic performance of dermoscopic algorithms and their individual criteria are not well studied. OBJECTIVES: To analyze the discriminatory power and reliability of dermoscopic criteria used in melanoma detection and compare the diagnostic accuracy of existing algorithms. DESIGN, SETTING, AND PARTICIPANTS: This was a retrospective, observational study of 477 lesions (119 melanomas [24.9%] and 358 nevi [75.1%]), which were divided into 12 image sets that consisted of 39 or 40 images per set. A link on the International Dermoscopy Society website from January 1, 2011, through December 31, 2011, directed participants to the study website. Data analysis was performed from June 1, 2013, through May 31, 2015. Participants included physicians, residents, and medical students, and there were no specialty-type or experience-level restrictions. Participants were randomly assigned to evaluate 1 of the 12 image sets. MAIN OUTCOMES AND MEASURES: Associations with melanoma and intraclass correlation coefficients (ICCs) were evaluated for the presence of dermoscopic criteria. Diagnostic accuracy measures were estimated for the following algorithms: the ABCD rule, the Menzies method, the 7-point checklist, the 3-point checklist, chaos and clues, and CASH (color, architecture, symmetry, and homogeneity). RESULTS: A total of 240 participants registered, and 103 (42.9%) evaluated all images. The 110 participants (45.8%) who evaluated fewer than 20 lesions were excluded, resulting in data from 130 participants (54.2%), 121 (93.1%) of whom were regular dermoscopy users. Criteria associated with melanoma included marked architectural disorder (odds ratio [OR], 6.6; 95% CI, 5.6-7.8), pattern asymmetry (OR, 4.9; 95% CI, 4.1-5.8), nonorganized pattern (OR, 3.3; 95% CI, 2.9-3.7), border score of 6 (OR, 3.3; 95% CI, 2.5-4.3), and contour asymmetry (OR, 3.2; 95% CI, 2.7-3.7) (P < .001 for all). Most dermoscopic criteria had poor to fair interobserver agreement. Criteria that reached moderate levels of agreement included comma vessels (ICC, 0.44; 95% CI, 0.40-0.49), absence of vessels (ICC, 0.46; 95% CI, 0.42-0.51), dark brown color (ICC, 0.40; 95% CI, 0.35-0.44), and architectural disorder (ICC, 0.43; 95% CI, 0.39-0.48). The Menzies method had the highest sensitivity for melanoma diagnosis (95.1%) but the lowest specificity (24.8%) compared with any other method (P < .001). The ABCD rule had the highest specificity (59.4%). All methods had similar areas under the receiver operating characteristic curves. CONCLUSIONS AND RELEVANCE: Important dermoscopic criteria for melanoma recognition were revalidated by participants with varied experience. Six algorithms tested had similar but modest levels of diagnostic accuracy, and the interobserver agreement of most individual criteria was poor

Validity and reliability of dermoscopic criteria used to differentiate nevi from melanoma aweb-based international dermoscopy society study

IMPORTANCE The comparative diagnostic performance of dermoscopic algorithms and their individual criteria are not well studied. OBJECTIVES To analyze the discriminatory power and reliability of dermoscopic criteria used in melanoma detection and compare the diagnostic accuracy of existing algorithms. DESIGN, SETTING, AND PARTICIPANTS Thiswas a retrospective, observational study of 477 lesions (119 melanomas [24.9%] and 358 nevi [75.1%]), which were divided into 12 image sets that consisted of 39 or 40 images per set. A link on the International Dermoscopy Society website from January 1, 2011, through December 31, 2011, directed participants to the study website. Data analysis was performed from June 1, 2013, through May 31, 2015. Participants included physicians, residents, and medical students, and there were no specialty-Type or experience-level restrictions. Participants were randomly assigned to evaluate 1 of the 12 image sets. MAIN OUTCOMES AND MEASURES Associations with melanoma and intraclass correlation coefficients (ICCs) were evaluated for the presence of dermoscopic criteria. Diagnostic accuracy measures were estimated for the following algorithms: The ABCD rule, the Menzies method, the 7-point checklist, the 3-point checklist, chaos and clues, and CASH (color, architecture, symmetry, and homogeneity). RESULTS A total of 240 participants registered, and 103 (42.9%) evaluated all images. The 110 participants (45.8%) who evaluated fewer than 20 lesions were excluded, resulting in data from 130 participants (54.2%), 121 (93.1%) of whom were regular dermoscopy users. Criteria associated with melanoma included marked architectural disorder (odds ratio [OR], 6.6; 95%CI, 5.6-7.8), pattern asymmetry (OR, 4.9; 95%CI, 4.1-5.8), nonorganized pattern (OR, 3.3; 95%CI, 2.9-3.7), border score of 6 (OR, 3.3; 95%CI, 2.5-4.3), and contour asymmetry (OR, 3.2; 95%CI, 2.7-3.7) (P &lt; .001 for all). Most dermoscopic criteria had poor to fair interobserver agreement. Criteria that reached moderate levels of agreement included comma vessels (ICC, 0.44; 95%CI, 0.40-0.49), absence of vessels (ICC, 0.46; 95%CI, 0.42-0.51), dark brown color (ICC, 0.40; 95%CI, 0.35-0.44), and architectural disorder (ICC, 0.43; 95%CI, 0.39-0.48). The Menziesmethod had the highest sensitivity for melanoma diagnosis (95.1%) but the lowest specificity (24.8%) compared with any other method (P &lt; .001). The ABCD rule had the highest specificity (59.4%). All methods had similar areas under the receiver operating characteristic curves. CONCLUSIONS AND RELEVANCE Important dermoscopic criteria for melanoma recognition were revalidated by participants with varied experience. Six algorithms tested had similar but modest levels of diagnostic accuracy, and the interobserver agreement of most individual criteria was poor