EBSD analysis of the structural state of the Cu-Zn-Mn-Al-Fe-Ni alloy after hot deformation

The structural state of the Cu-Zn-Mn-Al-Fe-Ni alloy after high-temperature forming is studied. It is reported that in the section of a ring blank there are two structural zones with different amounts of strain. It has been found by EBSD analysis that in the maximum strain zone the alloy suffers dynamic recrystallization under forming, whereas in the minimum strain zone there is only dynamic polygonization, coarse grain boundaries become strengthened, this being ac-companied by an increase in the values of hardness to 300 HV 0.2; this causes their embrittlement and brittle grain-boundary cracking of the blank at room temperature under the effect of residual stresses. © 2017 Author(s).The study was partially supported by the UB RAS basic research program, project No. 15-10-1-22. The research was done with the use of the equipment of the Plastometriya collective use center affiliated to IES UB RAS

The main psychological determinants of the participants’ readiness of professional skill competitions to perform successfully

The article presents the results of psychological research and the primary analysis of the results obtained, which allow us to formulate the main psychological determinants of the readiness of the contestants for competitions in professional skills and possible success. The study was conducted by means of questionnaire methods, primary statistical processing (percentage ratio), comparative analysis of the data obtained. The object of the study was the participants of professional skill competitions: winners, prize-winners and contestants who performed unsuccessfully. It was revealed that the winners and prize-winners of professional skill competitions, as well as participants who performed unsuccessfully, actively use psychological and professional techniques, techniques, creativity, and so on in preparation for competitions. The personal and professional qualities of the contestants contributing to their success and possible reasons for unsuccessful performances are noted. The main psychological determinants of the contestants’ readiness for competitions in professional skills and possible success are identified and formulated – personality strength; confidence; a high level of need for achievements and victories; an adequate level of self-esteem; a high level of reflection; competent knowledge of psychological technologies of self-action; a high level of personal and professional qualities development; rational independence in preparation

CTCF mediates chromatin looping via N-terminal domain-dependent cohesin retention

The DNA-binding protein CCCTC-binding factor (CTCF) and the cohesin complex function together to shape chromatin architecture in mammalian cells, but the molecular details of this process remain unclear. Here, we demonstrate that a 79-aa region within the CTCF N terminus is essential for cohesin positioning at CTCF binding sites and chromatin loop formation. However, the N terminus of CTCF fused to artificial zinc fingers was not sufficient to redirect cohesin to non-CTCF binding sites, indicating a lack of an autonomously functioning domain in CTCF responsible for cohesin positioning. BORIS (CTCFL), a germline-specific paralog of CTCF, was unable to anchor cohesin to CTCF DNA binding sites. Furthermore, CTCF-BORIS chimeric constructs provided evidence that, besides the N terminus of CTCF, the first two CTCF zinc fingers, and likely the 3D geometry of CTCF-DNA complexes, are also involved in cohesin retention. Based on this knowledge, we were able to convert BORIS into CTCF with respect to cohesin positioning, thus providing additional molecular details of the ability of CTCF to retain cohesin. Taken together, our data provide insight into the process by which DNA-bound CTCF constrains cohesin movement to shape spatiotemporal genome organization

The use of intermediate inserts for CO 2 laser welding of steel AISI 321 and a Grade 2 titanium alloy

The paper studies the structure, chemical and phase compositions, hardness and strength of welded joints obtained in AISI 321 steel and Grade 2 titanium alloy sheets by CO 2 continuous laser with the use of intermediate Cu, Ni and Ag-Cu-Zn alloy inserts. It is demonstrated that the maximum strength of welded joints is achieved by the welding conditions enabling one to form multiphase structures with intermetallics in the material of a weld, rather than only those based on solid solutions. © 2018 Author(s)

The Porous Structure of Copper-cadmium Oxide System Prepared by AC Electrochemical Synthesis

The porous structure of nanoparticles of copper-cadmium oxide system prepared by AC electrochemical synthesis in sodium chloride with a concentration of 3, 15, 25 wt. % has been studied. The obtained data indicate that at a higher current density and sodium chloride concentration of 3% wt. in the electrochemical oxidation of cadmium and copper products are formed with the structure of mesopores and channels. In this case, the specific surface area has the greatest value (19.4 m{2}/g) and a maximum pore volume (0.0778 cm{3}/g). Thus, the obtained data allow predicting the operating parameters of the electrolysis to obtain a copper - cadmium oxide system of a predetermined porous structure

Clinical manifestations and outcomes of tuberculosis in children in the Irkutsk region

100 children were observed. Tuberculosis of inrathoracic lymph nodes was found in 79 (79,0 %) patients, complicated tuberculosis - in 29 (36,7 %) of 79 patients. The phase of reverse development of tuberculosis was detected in 47,0 % of children with TB. Due to the slow dynamics of the disease, the duration of chemotherapy in 75,0 % of children was 240 days. In the mode of treatment, drug resistance of a patient in lesion of tuberculosis infection is taken into account. As a result of treatment, resorption of infiltration in the lungs was observed in 15,0 %, fibrosis - in 26,0 %, formation of small calcifications - in 39,0 %, formation of multiple large calcifications - in 20,0 % of children. The cause of the formation of calcifications is the late detection of tuberculosis. In children with the formation of calcifications throughout the course of treatment the number of leukocytes decreased, the downward trend in the absolute number of lymphocytes to the 4th month of treatment was traced, the content of leukocytes increased to the end of primary treatment. In children without formation of calcifications the reduction of leukocytes and lymphocytes was not identified during the treatment. The decrease in the content of leukocytes and lymphocytes may explain long-term persistence of endogenous intoxication

Medical and social characteristics of children with tuberculosis in conditions of social insufficiency in the Irkutsk region

Background. In cases of tuberculosis in children, contacts with patients with drug-resistant forms are often detected. Treatment and prevention of the disease is based on these data and adherence to treatment. Aims. To analyze the social status of the family of children with tuberculosis, clinical forms and drug resistance of MBT in patients who are sources of infection in the outbreak for adequate planning of preventive treatment of contact and treatment of children with tuberculosis. Materials and methods. The study involved 150 children with tuberculosis treated in a hospital in 2009-2012 and 142 children - in 2015-2017. We studied social factors and drug resistance of Mycobacterium tuberculosis in adult patients - sources to child transmission. Drug resistance of MBT cultures was determined by Löwenstein - Jensen medium and automated system BACTEC MGIT 960. Results. 50.0 % of children with tuberculosis are patients of preschool age, 33.0 % of children aged under 3 years. From 2009-2012 to 2015-2017 the proportion of socially disadvantaged families fell from 68.0 % to 45.1 %. At the same time the proportion of children with tuberculosis, contracted from a known contact decreased from 70.0 % to 57.0 %. Often children get infected from mother or from several close relatives (mother, father, grandfather, grandmother), TB patients source of infection of children are often diagnosed with infiltrative (21.9-38.3 %) and fibro-cavernous tuberculosis (17.0-21.0 %). To 2015-2017 multi-drug resistance in the nidi was recorded at 52.2 %. The development of tuberculosis in children was facilitated by the defects of preventative measures: absence of BCG vaccination (from 9.0 to 14.0 % of children), the absence of preventive treatment (55.3-67.5 %)

Feminitiviti: Nominative and Derivational Aspects

В статье рассматривается роль словообразования в изучении языковой картины мира применительно к сетевым неофеминитивам.The scientific novelty of the study is seen in the proof of the position on the role of word formation in the study of the linguistic picture of the world in relation to the group of neofeminitives

Regulation of the apoptotic genes in breast cancer cells by the transcription factor CTCF

CTCF is a highly conserved and ubiquitous transcription factor with versatile functions. We previously demonstrated that elevated protein levels of CTCF in breast cancer cells were associated with the specific anti-apoptotic function of CTCF. We used proteomics and microarray approaches to identify regulatory targets of CTCF specific for breast cancer cells. Among the CTCF identified targets were proteins involved in the control of apoptosis. A proapoptotic protein, Bax, negatively regulated by CTCF, was chosen for further investigation. Repression of the human Bax gene at the transcriptional level by CTCF in breast cancer cells was confirmed by real-time PCR. Two CTCF binding sites within the Bax promoter were identified by electrophoretic mobility shift assay and footprinting. In reporter assays, the Bax-luciferase reporter construct, containing CTCF-binding sites, was negatively regulated by CTCF. In vivo, CTCF occupied its binding sites in breast cancer cells and tissues, as confirmed by chromatin immunoprecipitation assay. Our findings suggest a possible mechanism of the specific CTCF anti-apoptotic function in breast cancer cells whereby CTCF is bound to the Bax promoter, resulting in repression of Bax and inhibition of apoptosis; depletion of CTCF leads to activation of Bax and apoptotic death. CTCF binding sites in the Bax promoter are unmethylated in all cells and tissues inspected. Therefore, specific CTCF interaction with the Bax promoter in breast cancer cells, and the functional outcome, may depend on a combination of epigenetic factors characteristic for these cells. Interestingly, CTCF appears to be a negative regulator of other proapoptotic genes (for example, Fas, Apaf-1, TP531NP1). Conversely, stimulating effects of CTCF on the anti-apoptotic genes (Bcl-2, Bag-3) have been observed. Taken together, these findings suggest that specific mechanisms have evolved in breast cancer cells to protect them from apoptosis; regulation of apoptotic genes by CTCF appears to be one of the resistance strategies