Optimization and economic evaluation of pressurized liquid extraction of phenolic compounds from jabuticaba skins

AbstractThe optimization of the extraction of anthocyanins and other phenolic compounds from jabuticaba skins, a promising Brazilian source of these compounds, was studied using pressurized liquid extraction (PLE). An optimization study was performed using ethanol as a solvent and with extraction pressure (5–10MPa), temperature (313–393K) and static extraction time (3–15min) as independent variables. The optimum PLE conditions for all response variables were estimated; however, PLE conditions resulting in the highest recovery of anthocyanins (5MPa, 553K and 9min of static extraction time) were chosen for comparison with a conventional low-pressure solvent extraction (LPSE). The attributes compared were yield, content of anthocyanins and phenolic compounds and economic feasibility. Similar extraction yields were obtained by LPSE and PLE under optimized conditions; however 2.15 and 1.66-fold more anthocyanins and total phenolic compounds, respectively, were extracted using PLE, while the cost of manufacturing (COM) obtained for the PLE extract was 40-fold lower

Obtaining phenolic compounds from jatoba (hymenaea courbaril L.) bark by supercritical fluid extraction

The extraction of polyphenol compounds from jatoba (Hymenaea courbaril L. var stilbocarpa) bark using supercritical fluid extraction (SFE) with CO 2 and cosolvents has been investigated. Among the solvent systems studied, SFE using CO2 and water (9:1, v/v), at 323 K and 35 MPa, presented the best results, with extract yield of 24%, and with high antioxidant activity (IC50 of 0.2 mg/cm3). This solvent system was used to determine global yield isotherms, which were built at 323 and 333 K, and 15, 25, and 35 MPa, using a second lot of jatoba. The highest yield was 11.5% at 15 MPa and 323 K, with maximum total phenolic compounds (TPC) of 335.00 mg TAE/g extract (d.b.) and total tannins content of 1.8 g/100 g raw material. A kinetic experiment was performed using optimized conditions, yielding 18% extract, and the kinetic parameters were used to scale-up the process from laboratory to pilot scale. Chemical analyses showed high content of phenolic compounds in the extracts of jatoba bark mostly due to the presence of procyanidins. © 2014 Elsevier B.V.The extraction of polyphenol compounds from jatoba (Hymenaea courbaril L. var stilbocarpa) bark using supercritical fluid extraction (SFE) with CO2 and cosolvents has been investigated. Among the solvent systems studied, SFE using CO2 and water (9:1, v/v)896877FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO2008/10986-2301301/2010-7De Albuquerque, U.P., De Medeiros, P.M., De Almeida, A.L.S., Monteiro, J.M., De Freitas Lins Neto, E.M., De Melo, J.G., Dos Santos, J.P., Medicinal plants of the caatinga (semi-arid) vegetation of NE Brazil: A quantitative approach (2007) Journal of Ethnopharmacology, 114 (3), pp. 325-354. , DOI 10.1016/j.jep.2007.08.017, PII S0378874107004229Calixto, J.B., Twenty-five years of research on medicinal plants in Latin America: A personal view (2005) Journal of Ethnopharmacology, 100 (1-2), pp. 131-134. , DOI 10.1016/j.jep.2005.06.004, PII S0378874105003612, Perspectives of EthnopharmacologyFernandes, T.T., Santos, A.T.F., Pimenta, F.C., Antimicrobial activity of Plathymenia reticulata, Hymenaea courbaril and Guazuma ulmifolia plants (2005) Revista de Patologia Tropical, 34, pp. 113-122Lorenzi, H., Matos, F.J.A., Medicinal plants in Brazil (2002) Native and Exotics, , Plantarum Institute Nova Odessa, SP, BrazilNogueira, R.T., Shepherd, G.J., Laverde, Jr.A., Marsaioli, A.J., Imamura, P.M., Clerodane-type diterpenes from the seed pods of Hymenaea courbaril var. Stilbocarpa (2001) Phytochemistry, 58 (8), pp. 1153-1157. , DOI 10.1016/S0031-9422(01)00303-X, PII S003194220100303XAguiar, J.C., Santiago, G.M., Lavor, P.L., Veras, H.N., Ferreira, Y.S., Lima, M.A., Arriaga, A.M., Braz-Filho, R., Chemical constituents and larvicidal activity of Hymenaea courbaril fruit peel (2010) Natural Product Communications, 5, pp. 1977-1980Abdel-Kader, M., Berger, J.M., Slebodnick, C., Hoch, J., Malone, S., Wisse, J.H., Werkhoven, M.C.M., Kingston, D.G.I., Isolation and absolute configuration of ent-halimane diterpenoids from Hymenaea courbaril from the Suriname rain forest (2002) Journal of Natural Products, 65 (1), pp. 11-15. , DOI 10.1021/np0103261Orsi, P.R., Bonamin, F., Severi, J.A., Santos, R.C., Vilegas, W., Hiruma-Lima, C.A., Stasi, L.C.D., Hymenaea stigonocarpa Mart. Ex Hayne: A Brazilian medicinal plant with gastric and duodenal anti-ulcer and antidiarrheal effects in experimental rodent models (2012) J. Ethnopharmacology, 143, pp. 81-90Braga, F.C., Wagner, H., Lombardi, J.A., De Oliveira, A.B., Screening Brazilian plant species for in vitro inhibition of 5-lipoxygenase (2000) Phytomedicine, 6, pp. 447-452Pettit, G.R., Meng, Y., Stevenson, C.A., Doubek, D.L., Knight, J.C., Cichacz, Z., Pettit, R.K., Schmidt, J.M., Isolation and structure of palstatin from the amazon tree Hymeneae palustris (2003) Journal of Natural Products, 66 (2), pp. 259-262. , DOI 10.1021/np020231eDoménech-Carbó, M.T., De La Cruz-Cañizares, J., Osete-Cortina, L., Doménech-Carbó, A., David, H., Ageing behaviour and analytical characterization of the Jatobá resin collected from Hymenaea stigonocarpa Mart (2009) International J. Mass Spectrometry, 284, pp. 81-92Sasaki, K., Matsukura, Y., Shijima, K., Miyake, M., Fujiwara, D., Konishi, Y., High-performance liquid chromatographic purification of oligomeric procyanidins, trimers up to nonamers, derived from the bark of Jatoba (Hymenaea courbaril) (2009) Bioscience, Biotechnology, and Biochemistry, 73, pp. 1274-1279Scalbert, A., Antimicrobial properties of tannins (1991) Phytochemistry, 30, pp. 3875-3883Monteiro, J.M., De Albuquerque, U.P., Araújo, E.L., De Amorim, E.L.C., (2005) Tannins from Chemistry to Ecology, 28, p. 4De Bruyne, T., Pieters, L., Deelstra, H., Vlietinck, A., Condensed vegetable tannins: Biodiversity in structure and biological activities (1999) Biochemical Systematics and Ecology, 27 (4), pp. 445-459. , DOI 10.1016/S0305-1978(98)00101-X, PII S030519789800101XChung, K.-T., Wong, T.Y., Wei, C.-I., Huang, Y.-W., Lin, Y., Tannins and human health: A review (1998) Critical Reviews in Food Science and Nutrition, 38 (6), pp. 421-464Shad, M.A., Nawaz, H., Rehman, T., Ahmad, H.B., Hussain, M., Optimization of extraction efficiency of tannins from Cichorium intybus L.: Application of response surface methodology (2012) J. Medicinal Plants Research, 6, pp. 4467-4474Pereira, C., Meireles, M.A., Supercritical fluid extraction of bioactive compounds: Fundamentals, applications and economic perspectives (2010) Food Bioprocess Technology, 3, pp. 340-372Serra, A.T., Seabra, I.J., Braga, M.E.M., Bronze, M.R., De Sousa, H.C., Duarte, C.M.M., Processing cherries (Prunus avium) using supercritical fluid technology. Part 1: Recovery of extract fractions rich in bioactive compounds (2010) J. Supercritical Fluids, 55, pp. 184-191Markom, M., Hasan, M., Daud, W.R.W., Singh, H., Jahim, J.M., Extraction of hydrolysable tannins from Phyllanthus niruri Linn.: Effects of solvents and extraction methods (2007) Separation and Purification Technology, 52 (3), pp. 487-496. , DOI 10.1016/j.seppur.2006.06.003, PII S1383586606001961Katayama, S., Zhao, L., Yonezawa, S., Iwai, Y., Modification of the surface of cotton with supercritical carbon dioxide and water to support nanoparticles (2012) J. Supercritical Fluids, 61, pp. 199-205Leitão, N.C.M.C.S., Prado, G.H.C., Veggi, P.C., Meireles, M.A.A., Pereira, C.G., Anacardium occidentale L. Leaves extraction via SFE: Global yields, extraction kinetics, mathematical modeling and economic evaluation (2013) J. Supercritical Fluids, 78, pp. 114-123Jacobs, M.B., (1973) The Chemical Analysis of Food and Products, , Eds. Publishing Co New York, NY(1998) Method of Determining and Expressing Fineness of Feed Materials by Sieving, 319 S. , ASAE-American Society of Agricultural Engineers American Society of Agricultural Engineers StandardBrunner, G., Gás extraction (1994) An Introduction to Fundamentals of Supercritical Fluids and the Application to Separation Process, , Springer Hamburgo, AlemanhaMeireles, M.A.A., Compounds from Latin American plants (2008) Supercritical Fluid Extraction of Nutraceuticals and Bioactive Compounds, pp. 243-274. , J. Martinez, CRC Press-Taylor & Francis Group Boca Raton, FLRodrigues, V.M., Sousa, E.M.B.D., Monteiro, A.R., Chiavone-Filho, O., Marques, M.O.M., Meireles, M.A.A., Determination of the solubility of extracts from vegetable raw material in pressurized CO2: A pseudo-ternary mixture formed by cellulosic structure + solute + solvent (2002) J. Supercritical Fluids, 22, pp. 21-36Prado, J.M., Prado, G.H.C., Meireles, M.A.A., Scale-up study of supercritical fluid extraction process for clove and sugarcane residue (2011) J. Supercritical Fluids, 56, pp. 231-237Kordali, S., Kotan, R., Mavi, A., Cakir, A., Ala, A., Yildirim, A., Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. Dracunculus, Artemisia santonicum, and Artemisia spicigera essential oils (2005) Journal of Agricultural and Food Chemistry, 53 (24), pp. 9452-9458. , DOI 10.1021/jf0516538Campos, P., Yariwake, J.H., Lancas, F.M., Effect of X- and gamma-rays on phenolic compounds from Maytenus aquifolium Martius (2005) Journal of Radioanalytical and Nuclear Chemistry, 264 (3), pp. 707-709. , DOI 10.1007/s10967-005-0775-9Martins, A.G., (1998) Influência de Fatores Tecnológicos Na Avaliação Analítica e Farmacológica de Extratos Secos de Maytenus Ilicifolia Martius Ex Reiss, p. 141. , School of Pharmacy and Chemistry, Federal University of Rio Grande do Sul Porto Alegre(2002) Atheneu Brazilian Pharmacopeia, , fourth ed. Atheneu São PauloBott, R.F., (2008) Influence of the Production Process, Storage Conditions and Physico-Chemical Stability on Dry Standardized Extracts of Medicinal Plants, p. 182. , School of Pharmaceutical Sciences of Ribeirão Preto, University of Sao Paulo Ribeirão PretoWagner, H., Bladt, S., (2001), Springer Berlin, AlemanhaIheozor-Ejiofor, P., Dey, E.S., Extraction of rosavin from Rhodiola rosea root using supercritical carbon dioxide with water (2009) J. Supercritical Fluids, 50, pp. 29-32Li, Q., Zhang, Z., Zhong, C., Liu, Y., Zhou, Q., Solubility of solid solutes in supercritical carbon dioxide with and without cosolvents (2003) Fluid Phase Equilibria, 207 (1-2), pp. 183-192. , DOI 10.1016/S0378-3812(03)00022-0Martinez-Correa, H.A., Cabral, F.A., Magalhães, P.M., Queiroga, C.L., Godoy, A.T., Sánchez-Camargo, A.P., Paviani, L.C., Extracts from the leaves of Baccharis dracunculifolia obtained by a combination of extraction processes with supercritical CO2, ethanol and water (2012) J. Supercritical Fluids, 63, pp. 31-39Santos, S.A.O., Villaverde, J.J., Silva, C.M., Neto, C.P., Silvestre, A.J.D., Supercritical fluid extraction of phenolic compounds from Eucalyptus globulus Labill bark (2012) J. Supercritical Fluids, 71, pp. 71-79Shan, B., Cai, Y.Z., Sun, M., Corke, H., Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents (2005) Journal of Agricultural and Food Chemistry, 53 (20), pp. 7749-7759. , DOI 10.1021/jf051513yVeggi, P.C., Santos, D.T., Fabiano-Tixier, A.-S., Bourvellec, C.L., Meireles, M.A.A., Chemat, F., Ultrasound-assisted extraction of polyphenols from Jatoba (Hymenaea courbaril L.var stilbocarpa) bark (2013) Food and Public Health, 3, p. 10Suzuki, R., Matsushita, Y., Imai, T., Sakurai, M., Henriques De Jesus, J., Ozaki, S., Finger, Z., Fukushima, K., Characterization and antioxidant activity of Amazonian woods (2008) J. Wood Science, 54, pp. 174-178Bezerra, G.P., Góis, R.W.D.S., Brito, T.S.D., Lima, F.J.B.D., Bandeira, M.A.M., Romero, N.R., Magalhães, P.J.C., Santiago, G.M.P., Phytochemical study guided by the myorelaxant activity of the crude extract, fractions and constituent from stem barkof Hymenaea courbaril L (2013) J. Ethnopharmacology, 149, p. 8Quispe-Condori, S., Sanchez, D., Foglio, M.A., Rosa, P.T.V., Zetzl, C., Brunner, G., Meireles, M.A.A., Global yield isotherms and kinetic of artemisinin extraction from Artemisia annua L leaves using supercritical carbon dioxide (2005) Journal of Supercritical Fluids, 36 (1), pp. 40-48. , DOI 10.1016/j.supflu.2005.03.003, PII S089684460500063XSilva, D.C.M.N., Bresciani, L.F.V., Dalagnol, R.L., Danielski, L., Yunes, R.A., Ferreira, S.R.S., Supercritical fluid extraction of carqueja (Baccharis trimera) oil: Process parameters and composition profiles (2009) Food and Bioproducts Processing, 87, pp. 317-326Murga, R., Ruiz, R., Beltran, S., Cabezas, J.L., Extraction of natural complex phenols and tannins from grape seeds by using supercritical mixtures of carbon dioxide and alcohol (2000) J. Agricultural and Food Chemistry, 48, pp. 3408-3412Yilmaz, E.E., Özvural, E.B., Vural, H., Extraction and identification of proanthocyanidins from grape seed (Vitis Vinifera) using supercritical carbon dioxide (2011) J. Supercritical Fluids, 55, pp. 924-928Palici, I., Tita, B., Ursica, L., Tita, D., Method for quantitative determination of polyphenolic compounds and tannins from vegetal products (2005) Seria F Chemia, 8, pp. 21-32Chavan, U.D., Shahidi, F., Naczk, M., Extraction of condensed tannins from beach pea (Lathyrus maritimus L.) as affected by different solvents (2001) Food Chemistry, 75, pp. 509-512Choi, Y.H., Kim, J.Y., Joung, S.N., Yoo, K.P., Chang, Y.S., Modifier effects on supercritical CO2 extraction efficiency of cephalotaxine from Cephalotaxus wilsoniana leaves (2000) Archives of Pharmacal Research, 23, pp. 163-166Çam, M., Hişil, Y., Pressurised water extraction of polyphenols from pomegranate peels (2010) Food Chemistry, 123, pp. 878-885Santos-Buelga, C., Scalbert, A., Proanthocyanidins and tannin-like compounds - Nature, occurrence, dietary intake and effects on nutrition and health (2000) Journal of the Science of Food and Agriculture, 80 (7), pp. 1094-1117. , DOI 10.1002/(SICI)1097-0010(20000515)80:73.0. CO;2-1Saad, H., Charrier-El Bouhtoury, F., Pizzi, A., Rode, K., Charrier, B., Ayed, N., Characterization of pomegranate peels tannin extractives (2012) Industrial Crops and Products, 40, pp. 239-246Rondeau, P., Gambier, F., Jolibert, F., Brosse, N., Compositions and chemical variability of grape pomaces from French vineyard (2013) Industrial Crops and Products, 43, pp. 251-254Pansera, M.R., Iob, G.A., Atti-Santos, A.C., Rossato, M., Atti-Serafini, L., Cassel, E., Extraction of tannin by Acacia mearnsii with supercritical fluids (2004) Brazilian Archives of Biology and Technology, 47 (6), pp. 995-998Prado, J.M., Dalmolin, I., Carareto, N.D.D., Basso, R.C., Meirelles, A.J.A., Vladimir Oliveira, J., Batista, E.A.C., Meireles, M.A.A., Supercritical fluid extraction of grape seed: Process scale-up, extract chemical composition and economic evaluation (2012) J. Food Engineering, 109, pp. 249-257Guilhon De Castro, H., De Oliveira, L.O., Barbosa, L.C.D.A., Ferreira, F.A., Henriques Da Silva, D.J., Mosquim, P.R., Nascimento, E.A., Content and composition of the essential oil of five accesses of mentrasto (2004) Quimica Nova, 27 (1), pp. 55-57Flores, P., Hellín, P., Fenoll, J., Determination of organic acids in fruits and vegetables by liquid chromatography with tandem-mass spectrometry (2012) Food Chemistry, 132, pp. 1049-1054Li, H., Song, F., Zheng, Z., Liu, Z., Liu, S., Characterization of saccharides and phenolic acids in the Chinese herb Tanshen by ESI-FT-ICR-MS and HPLC (2008) J. Mass Spectrometry, 43, pp. 1545-1552Paudel, L., Wyzgoski, F.J., Scheerens, J.C., Chanon, A.M., Reese, R.N., Smiljanic, D., Wesdemiotis, C., Rinaldi, P.L., Nonanthocyanin secondary metabolites of black raspberry (Rubus occidentalis L.) fruits: Identification by HPLC-DAD, NMR, HPLC-ESI-MS, and ESI-MS/MS analyses (2013) J. Agricultural and Food Chemistry, 61, pp. 12032-12043Fischer, U.A., Carle, R., Kammerer, D.R., Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD-ESI/MSn (2011) Food Chemistry, 127, pp. 807-821Ortega, N., Romero, M.-P., Macià, A., Reguant, J., Anglès, N., Morelló, J.-R., Motilva, M.-J., Comparative study of UPLC-MS/MS and HPLC-MS/MS to determine procyanidins and alkaloids in cocoa samples (2010) J. Food Composition and Analysis, 23, pp. 298-305Callemien, D., Guyot, S., Collin, S., Use of tiolysis hyphenated to RP-HPLC-ESI(-)-MS/MS for the analysis of flavonoids in fresh lager beers (2008) Food Chemistry, 110, pp. 1012-1018Gu, L., House, S.E., Wu, X., Ou, B., Prior, R.L., Procyanidin and catechin contents and antioxidant capacity of cocoa and chocolate products (2006) Journal of Agricultural and Food Chemistry, 54 (11), pp. 4057-4061. , DOI 10.1021/jf060360

Silk Fibroin Hydrogels Incorporated with the Antioxidant Extract of Stryphnodendron adstringens Bark

Barbatimão (Stryphnodendron adstringens) is a Brazilian medicinal plant known for its pharmacological properties, including healing activity related to its phenolic composition, which is chiefly given by tannins. In order to preserve its stability and bioactivity, barbatimão extracts can be incorporated into (bio-)polymeric matrixes, of which silk fibroin stands out due to its versatility and tunable properties. This work aimed to obtain barbatimão bark extract rich in phenolic compounds and evaluate its incorporation in fibroin hydrogels. From the extraction process, it was observed that the PG (propylene glycol) extract presented a higher global yield (X0) and phenolic compounds (TPC) than the ET (ethanol) extract. Furthermore, the antioxidant activity (ORAC and FRAP) was similar between both extracts. Regarding the hydrogels, morphological, chemical, thermal, and mechanical characterizations were performed to understand the influence of the barbatimão extract and the solvent on the fibroin hydrogel properties. As a result, the hydrogels containing the barbatimão PG extract (BT/PG hydrogels) showed the better physical–chemical and structural performance. Therefore, these hydrogels should be further investigated regarding their potential in medical and pharmaceutical applications, especially in wound healing

Guidelines on reporting treatment conditions for emerging technologies in food processing

In the last decades, different non-thermal and thermal technologies have been developed for food processing. However, in many cases, it is not clear which experimental parameters must be reported to guarantee the experiments’ reproducibility and provide the food industry a straightforward way to scale-up these technologies. Since reproducibility is one of the most important science features, the current work aims to improve the reproducibility of studies on emerging technologies for food processing by providing guidelines on reporting treatment conditions of thermal and non-thermal technologies. Infrared heating, microwave heating, ohmic heating and radiofrequency heating are addressed as advanced thermal technologies and isostatic high pressure, ultra-high-pressure homogenization sterilization, high-pressure homogenization, microfluidization, irradiation, plasma technologies, power ultrasound, pressure change technology, pulsed electric fields, pulsed light and supercritical CO2 are approached as non-thermal technologies. Finally, growing points and perspectives are highlighted