Exploring Sustainable Practices for Cold Storage for At-Risk Collections

This project will help plan for specialized storage for the fragile visual materials. These include photographs, negatives, moving images, and magnetic media that form a significant part of the rare and original archival collections housed in the Special Collections and Archives unit of the library. Funding from the National Endowment for the Humanities Sustaining Cultural Heritage Collections grant opportunity will bring in a team of specialized experts who will work collaboratively to plan for a storage environment that will significantly deter the degradation of these irreplaceable items

Identifying which septic patients have increased mortality risk using severity scores:a cohort study

Background: Early aggressive therapy can reduce the mortality associated with severe sepsis but this relies on prompt recognition, which is hindered by variation among published severity criteria. Our aim was to test the performance of different severity scores in predicting mortality among a cohort of hospital inpatients with sepsis. Methods: We anonymously linked routine outcome data to a cohort of prospectively identified adult hospital inpatients with sepsis, and used logistic regression to identify associations between mortality and demographic variables, clinical factors including blood culture results, and six sets of severity criteria. We calculated performance characteristics, including area under receiver operating characteristic curves (AUROC), of each set of severity criteria in predicting mortality. Results: Overall mortality was 19.4% (124/640) at 30 days after sepsis onset. In adjusted analysis, older age (odds ratio 5.79 (95% CI 2.87-11.70) for ≥80y versus <60y), having been admitted as an emergency (OR 3.91 (1.31-11.70) versus electively), and longer inpatient stay prior to sepsis onset (OR 2.90 (1.41-5.94) for >21d versus <4d), were associated with increased 30 day mortality. Being in a surgical or orthopaedic, versus medical, ward was associated with lower mortality (OR 0.47 (0.27-0.81) and 0.26 (0.11-0.63), respectively). Blood culture results (positive vs. negative) were not significantly association with mortality. All severity scores predicted mortality but performance varied. The CURB65 community-acquired pneumonia severity score had the best performance characteristics (sensitivity 81%, specificity 52%, positive predictive value 29%, negative predictive value 92%, for 30 day mortality), including having the largest AUROC curve (0.72, 95% CI 0.67-0.77). Conclusions: The CURB65 pneumonia severity score outperformed five other severity scores in predicting risk of death among a cohort of hospital inpatients with sepsis. The utility of the CURB65 score for risk-stratifying patients with sepsis in clinical practice will depend on replicating these findings in a validation cohort including patients with sepsis on admission to hospital

Movement bahaviour of three South African inshore sparid species in rocky intertidal and shallow subtidal habitats

This study investigated the movement behaviour of three inshore South African sparids – blacktail (Diplodus sargus capensis), zebra (Diplodus cervinus hottentotus) and white musselcracker (Sparodon durbanensis), popular inshore fishery species caught in appreciable numbers along much of the South African coast. The first study component examined movements of juveniles in a rocky intertidal nursery area at Schoenmakerskop near Port Elizabeth. Juveniles in a single gully were tagged with Visible Implant Elastomer (VIE) and resighted at the study site on snorkelling gear over a seven-month period. Tagged zebra and musselcracker displayed limited movement between potential low tide refuges, being observed repeatedly in the same gully over the full duration of the study. However, blacktail displayed greater movements and were seen infrequently in the later period of the study, probably having undergone an ontogenetic habitat shift to subtidal areas. There was evidence that blacktail maintain use of their intertidal nursery over high tide, during which the other two species moved into shallower areas adjacent to their low tide refuge. The results of three national tagging programs were analysed to determine the movement patterns of adolescent and adult fishes. The coast-wide ORI-WWF National Voluntary Tagging Program and two dedicated research programs in marine protected areas (MPAs) at De Hoop MPA and Tsitsikamma National Park (TNP) recorded few large-scale movements of tagged adult blacktail, zebra and juvenile musselcracker. High spatial-resolution data from the TNP suggested movements were usually on scales far smaller than one km. Together with long periods at liberty for many recaptured individuals, this suggests these fishes are longterm residents of small home range areas. However, tagged musselcracker over 600 mm forklength (adults) were observed to make large-scale movements, including some in excess of 800 km from Eastern Cape to KwaZulu-Natal waters. Predominantly eastward movements of adults recaptured during the spawning season indicate seasonal spawning migrations that occur in different regions of the coast. These enable the use of prevailing oceanographic currents to disperse eggs and larvae to suitable rocky nursery habitat. The third component of this study made use of high-resolution data on the temporal and spatial distribution of catches by scientific angling in the TNP to examine the daily activity patterns of the study species in relation to diel and tidal cycles, and habitat use. Blacktail capture probability was correlated with the diurnal light cycle, with peaks close to twilight suggesting elevated crepuscular foraging activity. Catches of blacktail, zebra and small musselcracker were correlated with the tidal cycle, foraging peaking over high tide periods. All three species used shallow inshore habitats extensively for foraging, blacktail showing a preference for sandy areas, while zebra and small musselcracker preferred shallow reef. Capture probability of larger musselcracker, however, was unrelated to habitat, possibly evidence of increasing area and habitat use with an ontogenetic change in diet. The lifetime movement patterns of these three species are discussed in relation to conservation measures and their management in South African fisheries. Restricted movement throughout post-settlement life for blacktail and zebra, and during the juvenile phase for musselcracker, makes local populations vulnerable to overexploitation. At present, MPAs probably play an important role in protecting local blacktail and zebra populations from overexploitation, and limited post-settlement movements mean the degree of larval dispersal between protected and adjacent areas will likely determine the effectiveness of MPAs in enhancing fisheries for these species. By contrast, MPAs likely provide recruits to fisheries for musselcracker during ontogenetic movements and dispersal from MPAs during spawning migrations. MPAs only offer partial protection to adult musselcracker populations in the spawning season, but this could nevertheless be significant under high levels of exploitation

Discovery of an X-ray pulsar in the low-mass X-ray binary 2A 1822-371

We report the discovery of 0.59 s X-ray pulsations from the low-mass X-ray binary, 5.57 hr dipping and eclipsing ADC source 2A 1822-371. Pulse arrival time analysis indicates a circular orbit with e < 0.03 (95% confidence) and an asini for the neutron star of 1.006(5) lightseconds, implying a mass function of (2.03+-0.03) x 10^-2 M_sun. The barycentric pulse period was 0.59325(2) s in 1996.270 and 0.59308615(5) s in 1998.205, indicating an average spin up with P_dot/P = (-1.52+-0.02) x 10^-4 yr^-1. For a magnetic field strength of ~1--5 x 10^12 G as derived from the X-ray spectrum the implied intrinsic X-ray luminosity is ~2-4 x 10^37 erg s^-1. The pulse amplitude is low, but increases steeply as a function of energy from a sinusoidal amplitude of 0.25% in 2-5.4 keV to ~3% above 20 keV. We discuss the constraints on the masses of the companion star and the fact that several aspects of the energy spectrum are in qualitative accordance with that of a strongly magnetised neutron star.Comment: 6 pages long, including 4 figures, uses emulateapj5, accepted for ApJL, Replaced figure

Repetitive Stimulation of the Pituitary with Growth-Hormone-Releasing Hormone Alters the Proportion of 22 and 20 Kilodalton Human-Growth Hormone Released

Background/Aims. 20 Kilodalton-hGH (20 K-hGH) is the second most abundant pituitary GH variant after 22 K-hGH. In the steady state the proportion of 20 : 22 K-hGH appears constant; does this proportion change with repetitive somatotroph stimulation? Methods. Forty adult males were randomised to receive a GHRH(1–29)NH2 bolus (0.5 μg/kg (n = 20) or 1.0 μg/kg (n = 20)), preceded or followed by a saline bolus, 1 week apart. Four to six weeks later, 10 subjects received 0.5 μg/kg GHRH(1–29)NH2 at 0, 60, 120, and 180 minutes. Clearance rate of 22 and 20 K-hGH was measured in 10 subjects. Results. Total amount/proportion of 22 K-hGH/20 K-hGH secreted was similar for both GHRH(1–29)NH2 doses. Repetitive stimulation reduced the amount of 22 K-hGH released whereas the amount of 20 K-hGH did not change significantly leading to an increase in the proportion of 20 K-hGH (P = .05). Half-life of 20 and 22 K-hGH were not significantly different (P = .55). Conclusions. Repetitive stimulation of the somatotroph may alter the proportion of GH variant released

Creating a Sport and Exercise Medicine Masters syllabus for doctors: a Delphi study

Objective Sport and Exercise Medicine (SEM) Masters curricula vary. This Delphi study is aimed to create a consensus curriculum for doctors undertaking SEM Masters courses. Methods A modified Delphi survey was used. An expert panel was established of individuals deemed to have adequate knowledge of the field. The research group developed the initial draft of the curriculum by collating and reviewing previously published UK-based postgraduate SEM-related curricula. There were two phases. In phase 1 the expert group either accepted, rejected or modified each learning objective (LO). During phase 2 the expert group were asked to accept or reject each LO that did not get accepted outright previously. The research group analysed the levels of agreements and the comments given by the expert panel after each phase. Results The expert panel consisted of 45 individuals, with 35 completing phase 2 (78% retention rate). Of the 136 LOs initially collated: 71 (52%) were accepted outright, 60 (44%) were altered in some way and reincluded in phase 2, and 5 (4%) were removed after phase 1. The research group added 2 (1%) new LOs on reflection over comments made by the expert panel. The final curriculum contained 133 LOs, divided into 11 subthemes. Conclusions The findings will better inform educators when developing SEM Masters curricula and inform students what they should look for when considering an SEM Masters. This consensus curriculum is an important step in standardising postgraduate SEM education

Mouse parotid salivary gland organoids for the in vitro study of stem cell radiation response

Objective: Hyposalivation-related xerostomia is an irreversible, untreatable, and frequent condition after radiotherapy for head and neck cancer. Stem cell therapy is an attractive option of treatment, but demands knowledge of stem cell functioning. Therefore, we aimed to develop a murine parotid gland organoid model to explore radiation response of stem cells in vitro. Materials and Methods: Single cells derived from murine parotid gland organoids were passaged in Matrigel with defined medium to assess self-renewal and differentiation potential. Single cells were irradiated and plated in a 3D clonogenic stem cell survival assay to assess submandibular and parotid gland radiation response. Results: Single cells derived from parotid gland organoids were able to extensively self-renew and differentiate into all major tissue cell types, indicating the presence of potential stem cells. FACS selection for known salivary gland stem cell markers CD24/CD29 did not further enrich for stem cells. The parotid gland organoid-derived stem cells displayed radiation dose–response curves similar to the submandibular gland. Conclusions: Murine parotid gland organoids harbor stem cells with long-term expansion and differentiation potential. This model is useful for mechanistic studies of stem cell radiation response and suggests similar radiosensitivity for the parotid and submandibular gland organoids

Using remote sensing to forecast forage quality for cattle in the dry savannas of northeast Australia

In the dry savannas of northeast Australia, forage quality is just as important for cattle production as forage quantity. The seasonal trend of forage quality is broadly predictable by land managers, but it is more difficult to predict the point when quality—which depends on local climate, management, and pasture condition—falls below the requirement for animal maintenance. In this study we use statistical modelling to forecast how forage quality might change at the crucial time of year, i.e., as the summer wet season transitions to the dry winter. We do this with the aid of historical information associated with a long-term cattle-grazing trial in the dry savannas. We combined multiple years of two measures of forage quality (dietary crude protein and in vivo dry-matter digestibility; respectively DCP and DMD) and ground cover information (specifically the ratio of ‘green grass’ cover to ‘dead (i.e., non-photosynthetic) grass’ cover, derived from an archive of Landsat satellite imagery) into a linear mixed model that explicitly considered the correlations with time and between variables. DCP and DMD were estimated by near-infrared spectroscopy of fresh faecal samples; values did not have to be temporally coincident with the satellite imagery. With the end of May considered a nominal decision-point, we forecast monthly averages of forage quality for June to August, over a 12-year period at the study site. Over all months and all years, the median absolute error of the forecasts was DCP = 0.86%, and DMD = 0.95%. The remote sensing information served as a correlated, oft-sampled covariate that helped to guide the forecasts of forage quality. We propose summarising the forecasts (and their uncertainty) as a near-real-time graphical tool for decision-support. Such a product could potentially benefit cattle-grazing enterprises in the northeast of Australia, enabling more timely management of herds through the dry season

Characterising the Gravitational Instability in Cooling Accretion Discs

We perform numerical analyses of the structure induced by gravitational instabilities in cooling gaseous accretion discs. For low enough cooling rates a quasi-steady configuration is reached, with the instability saturating at a finite amplitude in a marginally stable disc. We find that the saturation amplitude scales with the inverse square root of the cooling parameter beta = t_cool / t_dyn, which indicates that the heating rate induced by the instability is proportional to the energy density of the induced density waves. We find that at saturation the energy dissipated per dynamical time by weak shocks due is of the order of 20 per cent of the wave energy. From Fourier analysis of the disc structure we find that while the azimuthal wavenumber is roughly constant with radius, the mean radial wavenumber increases with radius, with the dominant mode corresponding to the locally most unstable wavelength. We demonstrate that the density waves excited in relatively low mass discs are always close to co-rotation, deviating from it by approximately 10 per cent. This can be understood in terms of the flow Doppler-shifted phase Mach number -- the pattern speed self-adjusts so that the flow into spiral arms is always sonic. This has profound effects on the degree to which transport through self-gravity can be modelled as a viscous process. Our results thus provide (a) a detailed description of how the self-regulation mechanism is established for low cooling rates, (b) a clarification of the conditions required for describing the transport induced by self-gravity through an effective viscosity, (c) an estimate of the maximum amplitude of the density perturbation before fragmentation occurs, and (d) a simple recipe to estimate the density perturbation in different thermal regimes.Comment: 16 pages, 22 figures. Accepted for publication in MNRAS 11 November 200