Smelling the goodness: sniffing as a behavioral measure of learned odor hedonics

Pairing an odor and taste can change ratings of the odor’s perceptual and hedonic characteristics. Behavioral indices of such changes are lacking and here we measured sniffing to assess learned changes in odor liking due to pairing with sweet and bitter tastes. Participants were divided on their liking for sweetness, as well as dietary disinhibition (TFEQ-D scale), both of which influence hedonic odor-taste learning. In sweet likers, both sniff duration and peak amplitude increased for the sweet-paired odor. Sniff magnitude decreased for sweet- and quinine-paired odors in sweet-dislikers, and sweet likers smelling the quinine-paired odor. In sweet-likers, liking for the sweet-paired odor increased with both TFEQ-D score and hunger, and sniff magnitude with TFEQ-D only. There were no predictors of changes in response to the quinine-paired odor. Brief co-experience of odors with sweet tastes can lead therefore to measurable changes in sniffing, providing a novel behavioral index of odor liking

The adenomatous polyposis coli protein unambiguously localizes to microtubule plus ends and is involved in establishing parallel arrays of microtubule bundles in highly polarized epithelial cells

Loss of full-length adenomatous polyposis coli (APC) protein correlates with the development of colon cancers in familial and sporadic cases. In addition to its role in regulating β-catenin levels in the Wnt signaling pathway, the APC protein is implicated in regulating cytoskeletal organization. APC stabilizes microtubules in vivo and in vitro, and this may play a role in cell migration (Näthke, I.S., C.L. Adams, P. Polakis, J.H. Sellin, and W.J. Nelson. 1996. J. Cell Biol. 134:165–179; Mimori-Kiyosue, Y., N. Shiina, and S. Tsukita. 2000. J. Cell Biol. 148:505–517; Zumbrunn, J., K. Inoshita, A.A. Hyman, and I.S. Näthke. 2001. Curr. Biol. 11:44–49) and in the attachment of microtubules to kinetochores during mitosis (Fodde, R., J. Kuipers, C. Rosenberg, R. Smits, M. Kielman, C. Gaspar, J.H. van Es, C. Breukel, J. Wiegant, R.H. Giles, and H. Clevers. 2001. Nat. Cell Biol. 3:433–438; Kaplan, K.B., A. Burds, J.R. Swedlow, S.S. Bekir, P.K. Sorger, and I.S. Näthke. 2001. Nat. Cell Biol. 3:429–432). The localization of endogenous APC protein is complex: actin- and microtubule-dependent pools of APC have been identified in cultured cells (Näthke et al., 1996; Mimori-Kiyosue et al., 2000; Reinacher-Schick, A., and B.M. Gumbiner. 2001. J. Cell Biol. 152:491–502; Rosin-Arbesfeld, R., G. Ihrke, and M. Bienz. 2001. EMBO J. 20:5929–5939). However, the localization of APC in tissues has not been identified at high resolution. Here, we show that in fully polarized epithelial cells from the inner ear, endogenous APC protein associates with the plus ends of microtubules located at the basal plasma membrane. Consistent with a role for APC in supporting the cytoskeletal organization of epithelial cells in vivo, the number of microtubules is significantly reduced in apico-basal arrays of microtubule bundles isolated from mice heterozygous for APC

TLR ligand-induced podosome disassembly in dendritic cells is ADAM17 dependent

Toll-like receptor (TLR) signaling induces a rapid reorganization of the actin cytoskeleton in cultured mouse dendritic cells (DC), leading to enhanced antigen endocytosis and a concomitant loss of filamentous actin–rich podosomes. We show that as podosomes are lost, TLR signaling induces prominent focal contacts and a transient reduction in DC migratory capacity in vitro. We further show that podosomes in mouse DC are foci of pronounced gelatinase activity, dependent on the enzyme membrane type I matrix metalloprotease (MT1-MMP), and that DC transiently lose the ability to degrade the extracellular matrix after TLR signaling. Surprisingly, MMP inhibitors block TLR signaling–induced podosome disassembly, although stimulated endocytosis is unaffected, which demonstrates that the two phenomena are not obligatorily coupled. Podosome disassembly caused by TLR signaling occurs normally in DC lacking MT1-MMP, and instead requires the tumor necrosis factor α–converting enzyme ADAM17 (a disintegrin and metalloprotease 17), which demonstrates a novel role for this “sheddase” in regulating an actin-based structure

A mechanistic inter-species comparison of flicker sensitivity

AbstractThe general validity of both the Rovamo [Vision Res. 39 (1999) 533] and Barten (Contrast sensitivity of the human eye, SPIE Optical Engineering Press, 1999), modulation transfer function models for describing flicker sensitivity in vertebrates was examined using published data for goldfish, chickens, tree shrews, ground squirrels, cats, pigeons and humans. Both models adequately described the flicker response in each species at frequencies greater than approximately 1 Hz. At lower frequencies, response predictions differed between the two models and this was due, in part, to dissimilar definitions of the role played by lateral inhibition in the retina. Modelled flicker sensitivity for a matched retinal illuminance condition enabled a direct inter-species comparison of signal processing response times at the photoreceptor level. The modelled results also quantified differences between species in post-retinal signal processing capability. Finally, the relationship between flicker frequency response curves and the perception of temporal signals in real visual scenes was examined for each species. It is proposed that the area under the flicker sensitivity function may offer a single “figure of merit” for specifying overall sensitivity to time signals in a species’ environment

Geology and Ground-Water Resources of Wichita and Greeley Counties, Kansas

This report describes the geography, geology, and ground-water resources of Wichita and Greeley counties in western Kansas. The area consists of a flat to gently rolling plain, which slopes eastward [at] about 15 feet per mile. A short reach of Ladder Creek (Beaver) is the only perennially flowing stream in the two counties. Ephemeral streams, which flow only during and after heavy rains, are White Woman and Sand Creeks and the western reach of Ladder Creek. The climate is semiarid, the normal annual precipitation being about 17 inches in Wichita County and 16 inches in Greeley County. Agriculture is the principal occupation in the area, and wheat is the most important crop. A considerable area is irrigated; sugar beets and sorghums are the principal irrigated crops. The outcropping rocks range in age from late Cretaceous to Recent; the Smoky Hill chalk member of the Niobrara formation, which is exposed along White Woman Creek in western Greeley County, is the oldest. The Niobrara is almost everywhere overlain by the Ogallala formation of Pliocene age. Generally the Ogallala is overlain by windblown silt of the Pleistocene Sanborn formation, but in places it is exposed along streams. The most recent deposits are dune sand and the alluvium along the streams. The Dakota formation, which is an important aquifer in parts of Kansas, is 300 to 450 feet beneath the Niobrara formation. The ground water that is available to wells in Wichita and Greeley counties is derived entirely from precipitation in the area or in areas immediately west and north. Ground water moves in a generally easterly direction with a gradient that varies inversely with the permeability of the water-bearing beds. The ground-water reservoir is recharged principally by precipitation within the area or within adjacent areas, Ground-water discharge takes place principally by pumping from wells, subsurface outflow, and evaporation and transpiration. Most of the domestic, stock, public, and irrigation supplies are obtained from wells. It is estimated that probably more than 2 billion gallons of water is pumped annually from wells in the area. Since 1947, ground-water recharge has been about equal to ground-water discharge. The use of ground water for irrigation has increased greatly since 1946 and indications are that many more wells may be drilled and pumped without dangerously lowering the water table. Approximately 11,000 to 12,000 acres were irrigated in 1951. A map showing the thickness of water-bearing materials indicates that although much of the area has enough water-bearing material to support irrigation wells, parts of Wichita and Greeley counties have little or none. The Ogallala is the principal water-bearing formation in the area. Small amounts of water may also be obtained locally from alluvial deposits and from cracks in the Niobrara formation. Two deep test wells to the Dakota formation have been drilled but, because of the poor quality of the water, have never been used. The ground water in Wichita and Greeley counties, though hard, is suitable for most purposes. Water from the Ogallala is generally high in fluoride and in some cases may be injurious to the teeth of children. Water from the Dakota, though soft, is unfit for irrigation because of a high content of sodium. The field data upon which most of this report is based are given in tables; they include records of 417 wells, chemical analyses of 31 samples of water, and logs of 57 test holes and wells

Recruitment to publicly funded trials - are surgical trials really different?

Good recruitment is integral to the conduct of a high-quality randomised controlled trial. It has been suggested that recruitment is particularly difficult for evaluations of surgical interventions, a field in which there is a dearth of evidence from randomised comparisons. While there is anecdotal speculation to support the inference that recruitment to surgical trials is more challenging than for medical trials we are unaware of any formal assessment of this. In this paper, we compare recruitment to surgical and medical trials using a cohort of publicly funded trials. Data: Overall recruitment to trials was assessed using of a cohort of publicly funded trials (n = 114). Comparisons were made by using the Recruitment Index, a simple measure of recruitment activity for multicentre randomised controlled trials. Recruitment at the centre level was also investigated through three example surgical trials. Results: The Recruitment Index was found to be higher, though not statistically significantly, in the surgical group (n = 18, median = 38.0 IQR (10.7, 77.4)) versus (n = 81, median = 34.8 IQR (11.7, 98.0)) days per recruit for the medical group (median difference 1.7 (− 19.2, 25.1); p = 0.828). For the trials where the comparison was between a surgical and a medical intervention, the Recruitment Index was substantially higher (n = 6, 68.3 (23.5, 294.8)) versus (n = 93, 34.6 (11.7, 90.0); median difference 25.9 (− 35.5, 221.8); p = 0.291) for the other trials. Conclusions: There was no clear evidence that surgical trials differ from medical trials in terms of recruitment activity. There was, however, support for the inference that medical versus surgical trials are more difficult to recruit to. Formal exploration of the recruitment data through a modelling approach may go some way to tease out where important differences exist.The first author was supported by a Medical Research Council UK Fellowship.Peer reviewedAuthor versio

An internal model architecture for novelty detection: Implications for cerebellar and collicular roles in sensory processing

The cerebellum is thought to implement internal models for sensory prediction, but details of the underlying circuitry are currently obscure. We therefore investigated a specific example of internal-model based sensory prediction, namely detection of whisker contacts during whisking. Inputs from the vibrissae in rats can be affected by signals generated by whisker movement, a phenomenon also observable in whisking robots. Robot novelty-detection can be improved by adaptive noise-cancellation, in which an adaptive filter learns a forward model of the whisker plant that allows the sensory effects of whisking to be predicted and thus subtracted from the noisy sensory input. However, the forward model only uses information from an efference copy of the whisking commands. Here we show that the addition of sensory information from the whiskers allows the adaptive filter to learn a more complex internal model that performs more robustly than the forward model, particularly when the whisking-induced interference has a periodic structure. We then propose a neural equivalent of the circuitry required for adaptive novelty-detection in the robot, in which the role of the adaptive filter is carried out by the cerebellum, with the comparison of its output (an estimate of the self-induced interference) and the original vibrissal signal occurring in the superior colliculus, a structure noted for its central role in novelty detection. This proposal makes a specific prediction concerning the whisker-related functions of a region in cerebellar cortical zone A2 that in rats receives climbing fibre input from the superior colliculus (via the inferior olive). This region has not been observed in non-whisking animals such as cats and primates, and its functional role in vibrissal processing has hitherto remained mysterious. Further investigation of this system may throw light on how cerebellar-based internal models could be used in broader sensory, motor and cognitive contexts. © 2012 Anderson et al