29 research outputs found
A weak scientific basis for gaming disorder: let us err on the side of caution
We greatly appreciate the care and thought that is evident in the 10 commentaries that discuss our debate paper, the
majority of which argued in favor of a formalized ICD-11 gaming disorder. We agree that there are some people
whose play of video games is related to life problems. We believe that understanding this population and the nature
and severity of the problems they experience should be a focus area for future research. However, moving from
research construct to formal disorder requires a much stronger evidence base than we currently have. The burden of
evidence and the clinical utility should be extremely high, because there is a genuine risk of abuse of diagnoses. We
provide suggestions about the level of evidence that might be required: transparent and preregistered studies, a better
demarcation of the subject area that includes a rationale for focusing on gaming particularly versus a more general
behavioral addictions concept, the exploration of non-addiction approaches, and the unbiased exploration of clinical
approaches that treat potentially underlying issues, such as depressive mood or social anxiety first. We acknowledge
there could be benefits to formalizing gaming disorder, many of which were highlighted by colleagues in their
commentaries, but we think they do not yet outweigh the wider societal and public health risks involved. Given the
gravity of diagnostic classification and its wider societal impact, we urge our colleagues at the WHO to err on the side
of caution for now and postpone the formalization
Control of the LHC 400 MHz RF System (ACS)
The LHC ACS RF system is composed of 16 superconducting cavities, eight per ring. Each ring has two cryomodules, each containing four cavities. Each cavity is powered by a 300 kW klystron. The klystrons are grouped in fours, the klystrons in each group sharing a common 58 kV power converter and HV equipment bunker. The ACS RF control system is based on modern industrial programmable controllers (PLCs). A new fast interlock and alarm system with inbuilt diagnostics has been developed. Extensive use of the FIPIO Fieldbus drastically decreases the cabling complexity and brings improved signal quality, increased reliability and easier maintenance. Features of the implementation, such as system layout, communication and the high-level software interface are described. Operational facilities such as the automatic switch on procedure are described, as well as the necessary specialist tools and interfaces. A complete RF chain, including high voltage, cryomodule and klystron is presently being assembled in order to check, as far as possible, all aspects of RF system operation before LHC installation. The experience gained so far in this test chain with the new control system is presented
Excretion of cytoplasmic proteins (ECP) in Staphylococcus aureus
Excretion of cytoplasmic proteins (ECP) is a common physiological feature in bacteria and eukaryotes. However, how these proteins without a typical signal peptide are excreted in bacteria is poorly understood. We studied the excretion pattern of cytoplasmic proteins using two glycolytic model enzymes, aldolase and enolase, and show that their excretion takes place mainly during the exponential growth phase in Staphylococcus aureus very similar to that of Sbi, an IgG-binding protein, which is secreted via the Sec-pathway. The amount of excreted enolase is substantial and is comparable with that of Sbi. For localization of the exit site, we fused aldolase and enolase with the peptidoglycan-binding motif, LysM, to trap the enzymes at the cell wall. With both immune fluorescence labeling and immunogold localization on electron microscopic thin sections aldolase and enolase were found apart from the cytoplasmic area particularly in the cross wall and at the septal cleft of dividing cells, whereas the non-excreted Ndh2, a soluble NADH:quinone oxidoreductase, is only seen attached to the inner side of the cytoplasmic membrane. The selectivity, the timing and the localization suggest that ECP is not a result of unspecific cell lysis but is mediated by an as yet unknown mechanism