Methodological evolutions of Raman spectroscopy in art and archaeology

During the last decades, Raman spectroscopy has grown from research laboratories to a well-established approach that is increasingly often used in archaeometry and conservation science

Nutritional Surveillance in Tuscany: eating habits at breakfast, mid-morning and afternoon snacks among 8-9 y-old children

Introduction.The prevalence of overweight and obesity in children is rapidly increasing in many countries. For that it has been interesting to investigate the eating habits of 8-9 y-old Tuscany children by paying attention to their meals frequency per day and their food choices in total and in relation to children?s Body Mass Index (BMI) classes. In addition we considered some environment factors that could affect the children eating behaviours, such as mother?s BMI and their education level. Methods. A statistical sample of 3,076 (1,583 males, 1,493 females), 8-9 year-old school-children was collected; weight and height were measured using standardized personnel and instruments. BMI classes were calculated using Cole et al.?s cutoff for children and adolescents. In order to evaluate the consumption frequency of individual meals and various foods, a Food Frequency Questionnaire (FFQ) was used, which was completed by the children themselves at school. A self-administered questionnaire revealed the weight and height of parents and their educational levels. Three educational levels were established: high, medium and low. Results. The results showed that 92.3% of children ate breakfast from 4-7 times a week, the vast majority at home, while only 3% declared consuming breakfast never or almost never. The most preferred breakfast consisted of milk and biscuits for all children?s BMI classes. 95.9% of children reported having mid-morning snack at school; fruit juice and tea are the most frequently consumed liquid foods, and pizza, salami sandwiches and pre-packaged snacks are the most frequently consumed solid foods in all BMI classes. 93.6% ate afternoon snack for the most part at home, even if 12% of children reported consuming it elsewhere; fruit juice and tea with pizza, sandwiches and pre-packaged snacks are still the most highly consumed foods by all children?s BMI classes. The consumption frequency of breakfast (P inf. 0.001), mid-morning (P inf. 0.05) and afternoon snack (P inf. 0.05) of 8-9 y-old Tuscany children decrease with increase the children?s BMI classes. The same tendency may be noted for the consumption frequency of breakfast in relation to mother?s BMI (P inf. 0.05) and their education level (P inf. 0.05). This data strengthens the thesis that some home environments can affect the children?s eating behaviours. Conclusion. No substantial differences in food choices at the meals analyzed were determined among normal weight, over weight and obese children. Children of normal weight had a greater tendency to consume meals more regularly. Mother?s BMI and their education level can have influence on children?eating behaviours

Risk of second primary malignancy in breast cancer survivors: A nested population-based case-control study

Purpose: Evolving therapies have improved the prognoses of patients with breast cancer; and currently, the number of long-term survivors is continuously increasing. However, these patients are at increased risk of developing a second cancer. Thus, late side effects are becoming an important issue. In this study, we aimed to investigate whether patient and tumor characteristics, and treatment type correlate with secondary tumor risk. Methods: This case-control study included 305 patients with a diagnosed second malignancy after almost 6 months after the diagnosis of primary breast cancer and 1,525 controls (ratio 1:5 of cases to controls) from a population-based cohort of 6,325 women. The control patients were randomly selected from the cohort and matched to the cases according to age at diagnosis, calendar period of diagnosis, disease stage, and time of follow-up. Results: BRCA1 or BRCA2 mutation, human epidermal growth factor receptor 2 (HER2)+ status, chemotherapy, and radiotherapy were related to increased risk of developing a second cancer, whereas hormonotherapy showed a protective effect. Chemotherapy, radiotherapy, and estrogenic receptor level <10% increased the risk of controlateral breast cancer. HER2+ status increased the risk of digestive system and thyroid tumors, while BRCA1 or BRCA2 mutation increased the risk of cancer in the genital system. Conclusion: Breast cancer survivors are exposed to an excess of risk of developing a second primary cancer. The development of excess of malignancies may be related either to patient and tumor characteristics, such as BRCA1 or BRCA2 mutation and HER2+ status, or to treatments factors

Aproveitamento pelo milho do nitrogênio amoniacal de dejetos líquidos de suínos em plantio direto e preparo reduzido do solo

The objective of this work was to evaluate corn (Zea mays) utilization of ammoniacal N from pig slurry (PS). A field experiment was carried out in a typic Hapludalf. The treatments consisted of the application or not of PS on oat straw, in no-tillage and reduced tillage planting systems. In order to quantify the destination of applied ammoniacal N from the PS, the ammoniacal fraction was enriched with (15NH4)2SO4. The utilization of ammoniacal N from pig slurry by corn crop was of 15.3% and did not differ between no-tillage and reduced tillage soil systems. At corn physiological maturity, the amount of 15N recovered from the soil, at 120-cm depth, and from the plant (grain+shoot+roots) was 49.6% of the 15N applied with pig slurry. The organic N fractions from pig slurry and from soil organic matter were the main sources of N for corn.O objetivo deste trabalho foi avaliar o aproveitamento do N amoniacal de dejetos líquidos de suínos (DLS) pela cultura do milho (Zea mays). O experimento foi conduzido em Argissolo Vermelho distrófico arênico. Os tratamentos avaliados consistiram da aplicação ou não de DLS sobre resíduos culturais de aveia, em plantio direto e preparo reduzido do solo. Para quantificar o destino do N amoniacal aplicado, a fração amoniacal dos DLS foi enriquecida com (15NH4)2SO4. O aproveitamento do N amoniacal dos DLS pelo milho foi de apenas 15,3% e não diferiu com o uso do DLS em plantio direto ou em preparo reduzido do solo. Na maturação fisiológica do milho, a quantidade de 15N dos DLS recuperada no solo, até a profundidade de 120 cm, e na planta (parte aérea+grãos+raízes) correspondeu a 49,6% do 15N aplicado. A fração de N orgânico dos DLS e a matéria orgânica do solo foram as principais fontes de N para a cultura do milho

Yerba Mate soluble como inhibidor de la corrosión del Zinc en medio ácido

Con el fin de encontrar inhibidores de corrosión que sean ambientalmente seguros y fácilmente disponibles, ha habido una tendencia creciente en el uso de productos naturales como hojas o extractos de plantas como inhibidores de corrosión de metales en procesos de decapado. El presente trabajo tiene como objetivo evaluar la eficiencia de la Yerba Mate soluble como inhibidor de la corrosión de zinc en una solución de HCl 0,1M. Se realizaron ensayos de pérdida de peso, polarización potenciodinámica y espectroscopía de impedancia electroquímica. Las experiencias se llevaron a cabo en ausencia y presencia del inhibidor (0,064, 0,124 y 0,248 g de yerba mate/L de solución), a diferentes temperaturas: 293K, 308K, 315K y 323K. Los resultados obtenidos en los ensayos de impedancia, se ajustaron según un circuito equivalente R(RQ). La eficiencia del inhibidor aumenta con el aumento de concentración del inhibidor. La Yerba Mate soluble actúa como un inhibidor mixto y la adsorción del mismo sobre la superficie del zinc se encuentra en concordancia con la isoterma de adsorción de Frumkin.For find corrosion inhibitors that are environmentally safe and readily available the use of natural products, such as leaves or plant extracts as metal corrosion inhibitors has been growing. The objetive of present work is to evaluate the efficiency of soluble Yerba Mate as a zinc corrosion inhibitor in a 0.1M HCl solution. Weight loss, potentiodynamic polarization and electrochemical impedance spectroscopy tests were carried out. The experiments were carried out with and without of the inhibitor, at concentrations of inhibitor of 0.064, 0.124 and 0.248 g of yerba mate/L of solution, at different temperatures: 293 K, 308 K, 315 K and 323 K. The results obtained in the impedance tests were adjusted according to an equivalent circuit R(RQ). The inhibitor efficiency increases with increasing inhibitor concentration. Soluble Yerba Mate acts as a mixed inhibitor and its adsorption on the zinc surface is in agreement with the Frumkin adsorption isotherm.Fil: Silva, Paula Belen. Universidad Nacional de Misiones; ArgentinaFil: Pozzi, Gonzalo. Universidad Nacional de Misiones; ArgentinaFil: Ares, Alicia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; ArgentinaFil: Mendez, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentin

The impact of the competence quorum sensing system on Streptococcus pneumoniae biofilms varies depending on the experimental model

Background: Different models for biofilm in Streptococcus pneumoniae have been described in literature. To permit comparison of experimental data, we characterised the impact of the pneumococcal quorum-sensing competence system on biofilm formation in three models. For this scope, we used two microtiter and one continuous culture biofilm system. Results: In both microtiter models the competence system influences stability and structure of biofilm in the late attachment phase and synthetic competence stimulating peptide (CSP) restored wild type phenotypes in the comC mutants unable to produce the peptide. Early attachment of single cells to well bottoms was found for both systems to be competence independent, while later phases, including microcolony formation correlated to an intact competence system. The continuous culture biofilm model was not affected by mutations in the competence locus, but deletion of capsule had a significant impact in this model. Conclusions: Since biofilm remains a largely uncharacterised multi-parameter phenotype it appears to be advisable to exploit more than one model in order to draw conclusion of possible relevance of specific genotypes on pneumococcal physiology.Claudia Trappetti, Luciana Gualdi, Lorenzo Di Meola, Prashant Jain, Cindy C Korir, Paul Edmonds, Francesco Iannelli, Susanna Ricci, Gianni Pozzi and Marco R Oggion

Egg-Independent Influenza Vaccines and Vaccine Candidates

Vaccination remains the principal way to control seasonal infections and is the most effective method of reducing influenza-associated morbidity and mortality. Since the 1940s, the main method of producing influenza vaccines has been an egg-based production process. However, in the event of a pandemic, this method has a significant limitation, as the time lag from strain isolation to final dose formulation and validation is six months. Indeed, production in eggs is a relatively slow process and production yields are both unpredictable and highly variable from strain to strain. In particular, if the next influenza pandemic were to arise from an avian influenza virus, and thus reduce the egg-laying hen population, there would be a shortage of embryonated eggs available for vaccine manufacturing. Although the production of egg-derived vaccines will continue, new technological developments have generated a cell-culture-based influenza vaccine and other more recent platforms, such as synthetic influenza vaccines

Medicine use and recurrent complaints among 15-years-old adolescents in Tuscany

Background.  The  use  of  medicines  to  treat  common  complaints,  such  as  headache, stomachache, difficulty in getting to sleep and nervousness, is widespread among ado-lescents,  and  is  increasing.  The  aim  of  this  survey  was  to  estimate  the  prevalence  of recurrent specific complaints among 15-year-old adolescents in Tuscany over three years(2006, 2010 and 2014) and that of medicine use to treat these complaints among boysand girls.Methods.The present study  is based on data from the Tuscan HBSC studies at threetime points (2006, 2010 and 2014), which involved 2830 adolescents, aged 15 years old(1395 boys and 1435 girls).Results.Overall, the data confirm that adolescents who suffer from recurrent complaintsare more likely to use medicines. The prevalence rates of all complaints were higher ingirls than in boys in the three years considered. Over the whole period, headache, stom-achache and difficulty in getting to sleep increased in girls, while boys displayed signifi-cant decrease in nervousness and stomachache.Conclusion.Our findings underline the fact that the use of medicines in adolescence isa public health concern and constitutes an emerging issue that needs greater attentionand investigation on the part of scientific researchBackground. The use of medicines to treat common complaints, such as headache, stomachache, difficulty in getting to sleep and nervousness, is widespread among adolescents, and is increasing. The aim of this survey was to estimate the prevalence of recurrent specific complaints among 15-year-old adolescents in Tuscany over three years (2006, 2010 and 2014) and that of medicine use to treat these complaints Conclusion. Our findings underline the fact that the use of medicines in adolescence is a public health concern and constitutes an emerging issue that needs greater attention and investigation on the part of scientific research

Ectonucleotidase activity and immunosuppression in astrocyte-CD4 T cell bidirectional signaling

Astrocytes play a crucial role in neuroinflammation as part of the glia limitans, which regulates infiltration of the brain parenchyma by leukocytes. The signaling pathways and molecular events, which result from the interaction of activated T cells with astrocytes are poorly defined. Here we show that astrocytes promote the expression and enzymatic activity of CD39 and CD73 ectonucleotidases in recently activated CD4 cells by a contact dependent mechanism that is independent of T cell receptor interaction with class II major histocompatibility complex (MHC). Transforming growth factor-β (TGF-β) is robustly upregulated and sufficient to promote ectonucleotidases expression. T cell adhesion to astrocyte results in differentiation to an immunosuppressive phenotype defined by expression of the transcription factor Rorγt, which characterizes the CD4 T helper 17 subset. CD39 activity in T cells in turn inhibits spontaneous calcium oscillations in astrocytes that correlated with enhanced and reduced transcription of CCL2 chemokine and Sonic hedgehog (Shh), respectively. We hypothesize this TCR-independent interaction promote an immunosuppressive program in T cells to control possible brain injury by deregulated T cell activation during neuroinflammation. On the other hand, the increased secretion of CCL2 with concomitant reduction of Shh might promote leukocytes extravasation into the brain parenchyma