Détermination des substrats lacustres par hydroacoustique : application au suivit de qualité morphologique

La diversité des écosystèmes lacustres s’explique en partie par la variété hydromorphologique des lacs ; la nature et la répartition du substrat qui tapisse leur fond sont des composantes de ce paramètre. C’est pour cela que la Directive Cadre sur l’Eau impose une description de la nature des sédiments des plans d’eau. Plus généralement, la répartition des substrats peut être considérée comme un facteur de structuration des espèces biologiques qu’abrite un lac. Des outils traditionnels comme l’utilisation d’une benne à sédiment ou d’une caméra subaquatique permettent de déterminer la nature des substrats de manière très ponctuelle mais ces techniques atteignent leurs limites lorsque tout un plan d’eau doit être caractérisé. Depuis les années 1980 des méthodes de caractérisation des sédiments utilisant des outils acoustiques qui permettent d’obtenir des informations en continu le long de parcours réalisés par un navire ont été développées et commercialisées. Pour l’application aux écosystèmes lacustres, de part leur mise en oeuvre, les systèmes utilisant les échosondeurs mono‐faisceau paraissent les plus appropriés. Ces appareils permettent de réaliser des cartes de la répartition des substrats à l’échelle du lac. Les informations pertinentes qui permettent de décrire un état biologique potentiel ou de définir un état initial peuvent être ainsi intégrer dans la mise en place des réseaux de suivi de la qualité des milieux. / The lake ecosystem diversity is explained, in part, by the hydromorphological diversity of lakes; nature and repartition of the substrata give information about this parameter. That is the reason why the European Water Framework imposes a substrata nature description of lakes. The substrata repartition could be considered as a factor of the biological structuring presents in lakes. Traditional tools like grab samplers or video cameras enable to determine the substrata nature but they are not appropriate for getting a high resolution description for an entire lake. From the beginning of the 80’s, acoustic devices specialized in seabed classification have been developed and commercialized. For lakebed surveys, systems using single beam sounders seem to be more appropriate; they enable to obtain maps of the lakebed at the whole lake scale. Information which describes a potential biological state can be used for the establishment of a quality monitoring

Knowledge Acquisition for the Creation of Assistance Tools to the Management of Air Traffic Control

International audienceThis paper presents an approach which has for objective to model new tools allowing to help the controllers to assume the incessant increase of the air traffic (actual version of the platform AMANDA V2), as well as help them in the negotiation phase and cooperation with their counterparts of adjacent sector (objectives of the new version of AMANDA). Help them in furnishing some tools able to quickly share information, and to maintain good common situation awareness. An approach is proposed, it is divided in three main phases. A first phase which consists of understand and to model the decision-making process of controllers. The second phase introduces a multiple criteria decision-making methodology. This Methodology has for objective to understand in more details the activities of controllers and the cases of cooperation with adjacent sectors. Finally, the last phase is the operational level of the approach, and consists of an application of repertory grid methodology in order to guide the interviews with the different participants of the study. This will allow realizing the knowledge acquisition, keeping in mind objective to develop new tools. To conclude this paper, the last part presents an example of application of this approach and the first results

AMANDA V3: Toward a Common Workspace between Air Traffic Controllers

International audienceThis paper presents the different tools developed in the LAMIH, in optics to assist air traffic controllers in their tasks, to decrease their workloads, and to enable them to support the ceaseless increase of the traffic. Common philosophy to all these tools is to preserve the controllers in the loop: we do not try to develop tools entirely automatic. The platform AMANDA V2 made it possible to set up and to evaluate a common workspace, which allows the two controllers of a sector to cooperate and to share the same representation of their traffic and conflicts. This space maintains common situation awareness. This tool was very appreciated by professional controllers and we now wish to extend this principle to the cooperation between two planning controllers of two adjacent sectors. It is what we present in this paper which begins with a presentation of the ATC then a point on the platforms of the laboratory and particularly AMANDA V2, to conclude with the objectives of AMANDA V3

Role of TLR1, TLR2 and TLR6 in the modulation of intestinal inflammation and Candida albicans elimination

Toll-like receptors (TLRs) are the major pattern recognition receptors that mediate sensing of a wide range of microorganisms. TLR2 forms heterodimers with either TLR1 or TLR6, broadening its ligand diversity against pathogens. TLR1, TLR2 and TLR6 have been implicated in the recognition of Candida albicans, an opportunistic fungal pathogen that colonizes the gastrointestinal tract. In this study, we explored whether the deficiency in TLR1, TLR2 or TLR6 impacts C. albicans colonization and inflammation-associated colonic injury in the dextran sulfate sodium (DSS)-induced colitis in mice. DSS treatment and C. albicans challenge induced greater weight loss, worse clinical signs of inflammation, higher histopathologic scores, and increased mortality rates in TLR1(-/-) and TLR2(-/-) mice when compared to TLR6(-/-) and wild-type mice. The number of C. albicans colonies in the stomach, colon and feces was decreased in TLR6(-/-) mice as compared to TLR2(-/-), TLR1(-/-) and wild-type mice. Interestingly, the population of E. coli in colonic luminal contents, intestinal permeability to FITC-dextran and cytokine expression were significantly increased in TLR1(-/-) and TLR2(-/-) mice, while they were decreased in TLR6(-/-) mice. In contrast to TLR6, both TLR1 and TLR2 deficiencies increased intestinal inflammation, and the overgrowth of C. albicans and E. coli populations in the colitis model, suggesting the involvement of TLR1 and TLR2 in epithelial homeostasis, and a role of TLR6 in increasing intestinal inflammation in response to pathogen-sensing

Vers une modélisation des systèmes coopératifs

International audienceDans le cadre de la coopération homme-homme, les agents humains construisent et maintiennent une représentation partagée de la situation par l'intermédiaire d'échanges verbaux. Cette représentation a été appelée Référentiel Commun, mais n'est que virtuelle. Dans le cadre de la coopération homme-machine, il est nécessaire de rendre opérationnel ce Référentiel Commun. C'est ce que nous présentons dans ce papier, où une expérimentation a permis de définir de manière exacte son contenu dans le cadre du contrôle de trafic aérien, ce qui a permis de bâtir un modèle de système coopératif que nous présentons ici

Landscape epidemiology of Batrachochytrium salamandrivorans : reconciling data limitations and conservation urgency

Starting in 2010, rapid-fire salamander (Salamandra salamandra) population declines in northwestern Europe heralded the emergence of Batrachochytrium salamandrivorans (Bsal), a salamander-pathogenic chytrid fungus. Bsal poses an imminent threat to global salamander diversity owing to its wide host range, high pathogenicity, and long-term persistence in ecosystems. While there is a pressing need to develop further research and conservation actions, data limitations inherent to recent pathogen emergence obscure necessary insights into Bsal disease ecology. Here, we use a hierarchical modeling framework to describe Bsal landscape epidemiology of outbreak sites in light of these methodological challenges. Using model selection and machine learning, we find that Bsal presence is associated with humid and relatively cool, stable climates. Outbreaks are generally located in areas characterized by low landscape heterogeneity and low steepness of slope. We further find an association between Bsal presence and high trail density, suggesting that human-mediated spread may increase risk for spillover between populations. We then use distribution modeling to show that favorable conditions occur in lowlands influenced by the North Sea, where increased survey effort is needed to determine how Bsal impacts local newt populations, but also in hill- and mountain ranges in northeastern France and the lower half of Germany. Finally, connectivity analyses suggest that these hill- and mountain ranges may act as stepping stones for further spread southward. Our results provide initial insight into regional environmental conditions underlying Bsal epizootics, present updated invasibility predictions for northwestern Europe, and lead us to discuss a wide variety of potential survey and research actions needed to advance future conservation and mitigation efforts

Deciphering the genome structure and paleohistory of _Theobroma cacao_

We sequenced and assembled the genome of _Theobroma cacao_, an economically important tropical fruit tree crop that is the source of chocolate. The assembly corresponds to 76% of the estimated genome size and contains almost all previously described genes, with 82% of them anchored on the 10 _T. cacao_ chromosomes. Analysis of this sequence information highlighted specific expansion of some gene families during evolution, for example flavonoid-related genes. It also provides a major source of candidate genes for _T. cacao_ disease resistance and quality improvement. Based on the inferred paleohistory of the T. cacao genome, we propose an evolutionary scenario whereby the ten _T. cacao_ chromosomes were shaped from an ancestor through eleven chromosome fusions. The _T. cacao_ genome can be considered as a simple living relic of higher plant evolution

Synaptic Maturation at Cortical Projections to the Lateral Amygdala in a Mouse Model of Rett Syndrome

Rett syndrome (RTT) is a neuro-developmental disorder caused by loss of function of Mecp2 - methyl-CpG-binding protein 2 - an epigenetic factor controlling DNA transcription. In mice, removal of Mecp2 in the forebrain recapitulates most of behavioral deficits found in global Mecp2 deficient mice, including amygdala-related hyper-anxiety and lack of social interaction, pointing a role of Mecp2 in emotional learning. Yet very little is known about the establishment and maintenance of synaptic function in the adult amygdala and the role of Mecp2 in these processes. Here, we performed a longitudinal examination of synaptic properties at excitatory projections to principal cells of the lateral nucleus of the amygdala (LA) in Mecp2 mutant mice and their wild-type littermates. We first show that during animal life, Cortico-LA projections switch from a tonic to a phasic mode, whereas Thalamo-LA synapses are phasic at all ages. In parallel, we observed a specific elimination of Cortico-LA synapses and a decrease in their ability of generating presynaptic long term potentiation. In absence of Mecp2, both synaptic maturation and synaptic elimination were exaggerated albeit still specific to cortical projections. Surprisingly, associative LTP was unaffected at Mecp2 deficient synapses suggesting that synaptic maintenance rather than activity-dependent synaptic learning may be causal in RTT physiopathology. Finally, because the timing of synaptic evolution was preserved, we propose that some of the developmental effects of Mecp2 may be exerted within an endogenous program and restricted to synapses which maturate during animal life

Analysis of the P. lividus sea urchin genome highlights contrasting trends of genomic and regulatory evolution in deuterostomes

Sea urchins are emblematic models in developmental biology and display several characteristics that set them apart from other deuterostomes. To uncover the genomic cues that may underlie these specificities, we generated a chromosome-scale genome assembly for the sea urchin Paracentrotus lividus and an extensive gene expression and epigenetic profiles of its embryonic development. We found that, unlike vertebrates, sea urchins retained ancestral chromosomal linkages but underwent very fast intrachromosomal gene order mixing. We identified a burst of gene duplication in the echinoid lineage and showed that some of these expanded genes have been recruited in novel structures (water vascular system, Aristotle's lantern, and skeletogenic micromere lineage). Finally, we identified gene-regulatory modules conserved between sea urchins and chordates. Our results suggest that gene-regulatory networks controlling development can be conserved despite extensive gene order rearrangement