Vergleich der Aktivierung von Signalkaskaden und Genen durch Oncostatin M in humanen Glioblastomzellen

Oncostatin M (OSM) und Leukemia Inhibitory Factor (LIF) sind zwei Zytokine, die Effekte auf Zellen durch Bindung an den Typ I OSM-Rezeptor auslösen können. OSM kann zusätzlich an den Typ II OSM-Rezeptor binden. Eine nur durch OSM induzierte, höchstwahrscheinlich über den Typ II OSM-Rezeptor übertragene, signifikante Wachstumsinhibition auf bestimmte Tumorzellinien ist bekannt. Der Vergleich aktivierter Signalmoleküle nach Stimulation mit OSM und LIF sollte diejenigen identifizieren, die an der Übertragung dieses Effektes beteiligt sein könnten. Die meisten Proteine der Jak/Stat- und der MAPK-Signalkaskaden wurden nach Stimulation mit OSM und LIF phosphoryliert. Stat5b wurde nur nach Behandlung der Zellen mit OSM aktiviert. Insgesamt zeigte sich eine wesentlich stärkere Tyrosinphosphorylierung der Proteine nach Stimulation mit OSM. Die Ergebnisse zeigen, dass die Aktivierung des Jak-Stat- und des MAPK-Signalweges nicht ausreichend ist, um eine Wachstumsinhibition zu induzieren

Preschool psychopathology reported by parents in 23 societies: testing the seven-syndrome model of the child behavior checklist for ages 1.5-5

Objective: To test the fit of a seven-syndrome model to ratings of preschoolers' problems by parents in very diverse societies. Method: Parents of 19,106 children 18 to 71 months of age from 23 societies in Asia, Australasia, Europe, the Middle East, and South America completed the Child Behavior Checklist for Ages 1.5-5 (CBCL/1.5-5). Confirmatory factor analyses were used to test the seven-syndrome model separately for each society. Results: The primary model fit index, the root mean square error of approximation (RMSEA), indicated acceptable to good fit for each society. Although a six-syndrome model combining the Emotionally Reactive and Anxious/Depressed syndromes also fit the data for nine societies, it fit less well than the seven-syndrome model for seven of the nine societies. Other fit indices yielded less consistent results than the RMSEA. Conclusions: The seven-syndrome model provides one way to capture patterns of children's problems that are manifested in ratings by parents from many societies. Clinicians working with preschoolers from these societies can thus assess and describe parents' ratings of behavioral, emotional, and social problems in terms of the seven syndromes. The results illustrate possibilities for culture general taxonomic constructs of preschool psychopathology. Problems not captured by the CBCL/1.5-5 may form additional syndromes, and other syndrome models may also fit the data.Dr. Ivanovo receives research and salary support from the Research Center for Children, Youth, and Families, which publishes the Child Behavior Checklist [CBCL]. Dr. Achenbach is President of the Research Center for Children, Youth, and Families, and receives remuneration. Dr. Rescorla receives remuneration from the Research Center for Children, Youth, and Families. Dr. Harder previously held a University of Vermont Postdoctoral Fellowship funding by the Research Center for Children, Youth, and Families. Drs. Bjarnadottir, Gudmundsson, Leung, Verhulst, and Mr. Gudmundsson, receive research support from the sole of the CBCL. Dr. Bilenberg has received honoraria from Eli Lilly and Co., Novartis, Neuroscience, and Janseen Cilag. He has received research support from the Danish Research Foundation, the Lundbeck Foundation, Hermansens Mindelegat, and Mods Clausen Fond. Dr. Rapes has received research support from the Sheikh Hamdan Bin Rashid Al Maktoum Award for Medical Sciences. Dr. Jusiene has received research support from the Lithuanian Science and Studies Foundation. Drs. Ang, Capron, Dias, Dobrean, Doepfner, Duyme, Erol, Esmaeili, Ezpeleta, Frigerio, Goncalves, Jung, Kim, Liu, Oh, Plueck, Pomo limo, Shahini, Silva, Simsek, Souronder, Valverde, Van Leeuwen, and Zubrick, Ms. De Paw, Ms. Kristensen, Mr. Lecannelier, Ms. Montirosso, Ms. Jetishi, Ms. Woo, and Ms. Wu report no biomedical financial interests or potential conflicts of interest.info:eu-repo/semantics/publishedVersio